NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF185
Library VH
(Link to library)
Clone ID VHF185
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16415-1
Original site URL
Representative seq. ID VHF185P
(Link to Original site)
Representative DNA sequence 
>VHF185 (VHF185Q) /CSM/VH/VHF1-D/VHF185Q.Seq.d/
ATATTTTTAAAAAATGGCAACAATTGAAGATACTTNAAAGATAATAACAATCCAATTTTA
GAACGTAAAAAAGCTCCAAATAGATTATTTGTAGAAGAAGCAATTAATGATGATAATTCA
GTAGTTACATTAAATCCAGAGACAATGGATCAATTACAATTCTTTAGAGGTGACACACTT
TTAATTAAAGGTAAAAAGAGAAGAGATACCGTTTGTATTGTATTGTCAGATCCAACCATC
GATCCATCAAAGATCCGTATGAATAAAGTTGTAAGAAATAATCTTCGTGTTAGATTAGGT
GACATGATTTCAGTTCACCAATGTTCAGATGTTAAATATGGCAAACGTATTCATGTGTTA
CCAATTGATGATACCATAGAAGGTCTCAGTGGCAATCTCTTTGATCTTTACCTCAAACCA
TACTTTTTAGAAGCCTACCGTCCAGTCCGTAAGGGTGACTTATTTTTAGTACGTGGTGGT
ATGAGAGCCGTAGAATTTAAAGTTGTCGAAACCGACCCAGGTGAATATTGTATCGTTGCA
CCAGAGACATTTATACATTGTGAAGGTGAGGCAGTCAAGAGAGAXXXXXXXXXXTATNAT
CGATCCAGCTATCNTCCGTCCAGGTNGTTTAGATCAACTCATTTACATTCCATTACCAGA
TTTACCATTTNGTGTTGCCATTTTCAAGGNTTGTTTCAATAAATCNCCAGTNGCCAAGGA
TGTAGATTTAGAATTTTTGGGTCAAAAGANTCAAGGTTTTTTTGGTGCAGATTTAACTGA
AATTTGTCAACGTGCCTGTAAATTAGCTANTNGTGAATCCATTGAAAAGGATATTGAATC
CNCCAAAGCCCGTCAAGAATTTGGTGATACCAANATGGAANANGAATNTGTCGATCCAGT
ACCAGAAATTAATNGTGATCNCTTCCAANAAGCCATGANATNTGNTNGTCGTTNTGTATC
CGATAATGATANTAGAAAATATGAAAGCTTTGCTCAAACTTTAGTTCAATCAAGAGGTCT
TGGTAANAATTTCAAATTCCCAGATCAAGAATCTTCAGGTC
sequence update 2002.10.25
Translated Amino Acid sequence 
iflkngnn*RYXKDNNNPILERKKAPNRLFVEEAINDDNSVVTLNPETMDQLQFFRGDTL
LIKGKKRRDTVCIVLSDPTIDPSKIRMNKVVRNNLRVRLGDMISVHQCSDVKYGKRIHVL
PIDDTIEGLSGNLFDLYLKPYFLEAYRPVRKGDLFLVRGGMRAVEFKVVETDPGEYCIVA
PETFIHCEGEAVKR---

---XIDPAIXRPGXLDQLIYIPLPDLPFXVAIFKXCFNKSPVAKDVDLEFLGQKXQGFFG
ADLTEICQRACKLAXXESIEKDIESXKARQEFGDTXMEXEXVDPVPEINXDXFQXAMXXX
XRXVSDNDXRKYESFAQTLVQSRGLGXNFKFPDQESSG
Translated Amino Acid sequence (All Frames) 
Frame A:
iflkngnn*RYXKDNNNPILERKKAPNRLFVEEAINDDNSVVTLNPETMDQLQFFRGDTL
LIKGKKRRDTVCIVLSDPTIDPSKIRMNKVVRNNLRVRLGDMISVHQCSDVKYGKRIHVL
PIDDTIEGLSGNLFDLYLKPYFLEAYRPVRKGDLFLVRGGMRAVEFKVVETDPGEYCIVA
PETFIHCEGEAVKR---

---yxrssyxpsrxfrsthlhsitrftixcchfqglfq*ixsxqgcrfrifgskxsrffw
crfn*nlstcl*isxx*ih*kgy*ixqspsriw*yqxgxxxcrsstrn*x*slpxshxxx
xsxcir**x*ki*klcsnfssikrsw*xfqiprsrifr

Frame B:
yf*kmatiedtxkiitiqf*nvkklqidyl*kkqlmmiiq*lh*iqrqwinynslevthf
*lkvkreeipfvlycqiqpsihqrsv*ikl*eiifvld*vt*fqftnvqmlnmanvfmcy
qlmip*kvsvaisliftsnhtf*kptvqsvrvtyf*yvvv*ep*nlklskptqvnivslh
qrhlyivkvrqsre---

---XIDPAIXRPGXLDQLIYIPLPDLPFXVAIFKXCFNKSPVAKDVDLEFLGQKXQGFFG
ADLTEICQRACKLAXXESIEKDIESXKARQEFGDTXMEXEXVDPVPEINXDXFQXAMXXX
XRXVSDNDXRKYESFAQTLVQSRGLGXNFKFPDQESSG

Frame C:
ifkkwqqlkilxr**qsnfrt*kssk*iicrrsn****fssyiksrdngsitil*r*htf
n*r*kekryrlycivrsnhrsikdpye*sckk*ssc*ir*hdfsspmfrc*iwqtyscvt
n**yhrrsqwqsl*slpqtilfrslpssp*g*lifstwwyesrri*scrnrpr*ilyrct
rdiytl*r*gsqer---

---xsiqlssvqvv*insftfhyqiyhxvlpfsrxvsinxqxprm*i*nfwvkrxkvflv
qi*lkfvnvpvn*lxvnplkrilnppkpvknlvipxwxxnxsiqyqklxvixsxkp*xxx
vvxypimixenmkallkl*fnqevlvxisnsqiknlqv
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF185 (VHF185Q) /CSM/VH/VHF1-D/VHF185Q.Seq.d/                       1848   0.0
VHJ543 (VHJ543Q) /CSM/VH/VHJ5-B/VHJ543Q.Seq.d/                       1152   0.0
VHH667 (VHH667Q) /CSM/VH/VHH6-C/VHH667Q.Seq.d/                       1152   0.0
VHC216 (VHC216Q) /CSM/VH/VHC2-A/VHC216Q.Seq.d/                       1152   0.0
SHD424 (SHD424Q) /CSM/SH/SHD4-A/SHD424Q.Seq.d/                       1152   0.0
CHR514 (CHR514Q) /CSM/CH/CHR5-A/CHR514Q.Seq.d/                       1152   0.0
CHK496 (CHK496Q) /CSM/CH/CHK4-D/CHK496Q.Seq.d/                       1152   0.0
CHK459 (CHK459Q) /CSM/CH/CHK4-C/CHK459Q.Seq.d/                       1152   0.0
AHO409 (AHO409Q) /CSM/AH/AHO4-A/AHO409Q.Seq.d/                       1152   0.0
AHO293 (AHO293Q) /CSM/AH/AHO2-D/AHO293Q.Seq.d/                       1152   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U83085|U83085.2 Dictyostelium discoideum cell division cycle protein 48 (cdcD) mRNA, complete cds.  1086   0.0    2
CN763635|CN763635.1 ID0AAA7CE05RM1 ApMS Acyrthosiphon pisum cDNA clone ID0AAA7CE05 5', mRNA sequence.    52   3e-20  4
CN586788|CN586788.1 USDA-FP_129860 Acyrthosiphon pisum, Pea Aphid Acyrthosiphon pisum cDNA clone WHAP089_H03, mRNA sequence.    52   3e-20  4
EC821281|EC821281.1 SME00000710 esmbsro2 Sawyeria marylandensis cDNA, mRNA sequence.    66   3e-19  3
EC822226|EC822226.1 SME00000007 esmbsro2 Sawyeria marylandensis cDNA, mRNA sequence.    66   3e-19  3
EC823170|EC823170.1 SME00001357 esmbsro2 Sawyeria marylandensis cDNA, mRNA sequence.    66   4e-19  3
EC824811|EC824811.1 SME00007123 esmbsro2 Sawyeria marylandensis cDNA, mRNA sequence.    66   4e-19  3
EC825123|EC825123.1 SME00001874 esmbsro2 Sawyeria marylandensis cDNA, mRNA sequence.    66   5e-19  3
EC826595|EC826595.1 SME00008362 esmbsro2 Sawyeria marylandensis cDNA, mRNA sequence.    66   6e-19  3
EE111940|EE111940.1 G142P60198RC7.T0 Acorn worm juvenile pCMVSport6 library Saccoglossus kowalevskii cDNA 5' end, mRNA sequence.    58   3e-16  5
dna update 2007.10.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U83085_1(U83085|pid:none) Dictyostelium discoideum cell division...   359   8e-98
DQ515925_1(DQ515925|pid:none) Nicotiana tabacum putative spindle...   300   4e-80
AL162751_7(AL162751|pid:none) Arabidopsis thaliana DNA chromosom...   299   1e-79
FB781796_1(FB781796|pid:none) Sequence 1069 from Patent WO200803...   297   5e-79
EU606206_1(EU606206|pid:none) Dimocarpus longan cell division cy...   296   6e-79
GM966461_1(GM966461|pid:none) Sequence 1793 from Patent WO200814...   295   1e-78
FB781494_1(FB781494|pid:none) Sequence 767 from Patent WO2008034...   295   1e-78
EU961444_1(EU961444|pid:none) Zea mays clone 235510 unknown mRNA.     295   2e-78
FB905989_1(FB905989|pid:none) Sequence 125262 from Patent WO2008...   295   2e-78
FB781564_1(FB781564|pid:none) Sequence 837 from Patent WO2008034...   295   2e-78
protein update 2009. 7. 2
PSORT 

 psg: 0.45  gvh: 0.33  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.07  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 32.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHF185 is nuc
5' end seq. ID VHF185F
5' end seq. 
>VHF185F.Seq
ATATTTTTAAAAAATGGCAACAATTGAAGATACTTNAAAGATAATAACAATCCAATTTTA
GAACGTAAAAAAGCTCCAAATAGATTATTTGTAGAAGAAGCAATTAATGATGATAATTCA
GTAGTTACATTAAATCCAGAGACAATGGATCAATTACAATTCTTTAGAGGTGACACACTT
TTAATTAAAGGTAAAAAGAGAAGAGATACCGTTTGTATTGTATTGTCAGATCCAACCATC
GATCCATCAAAGATCCGTATGAATAAAGTTGTAAGAAATAATCTTCGTGTTAGATTAGGT
GACATGATTTCAGTTCACCAATGTTCAGATGTTAAATATGGCAAACGTATTCATGTGTTA
CCAATTGATGATACCATAGAAGGTCTCAGTGGCAATCTCTTTGATCTTTACCTCAAACCA
TACTTTTTAGAAGCCTACCGTCCAGTCCGTAAGGGTGACTTATTTTTAGTACGTGGTGGT
ATGAGAGCCGTAGAATTTAAAGTTGTCGAAACCGACCCAGGTGAATATTGTATCGTTGCA
CCAGAGACATTTATACATTGTGAAGGTGAGGCAGTCAAGAGAGANNNNNNNNNN
Length of 5' end seq. 594
3' end seq. ID VHF185Z
3' end seq. 
>VHF185Z.Seq
NNNNNNNNNNTATNATCGATCCAGCTATCNTCCGTCCAGGTNGTTTAGATCAACTCATTT
ACATTCCATTACCAGATTTACCATTTNGTGTTGCCATTTTCAAGGNTTGTTTCAATAAAT
CNCCAGTNGCCAAGGATGTAGATTTAGAATTTTTGGGTCAAAAGANTCAAGGTTTTTTTG
GTGCAGATTTAACTGAAATTTGTCAACGTGCCTGTAAATTAGCTANTNGTGAATCCATTG
AAAAGGATATTGAATCCNCCAAAGCCCGTCAAGAATTTGGTGATACCAANATGGAANANG
AATNTGTCGATCCAGTACCAGAAATTAATNGTGATCNCTTCCAANAAGCCATGANATNTG
NTNGTCGTTNTGTATCCGATAATGATANTAGAAAATATGAAAGCTTTGCTCAAACTTTAG
TTCAATCAAGAGGTCTTGGTAANAATTTCAAATTCCCAGATCAAGAATCTTCAGGTC
Length of 3' end seq. 477
Connected seq. ID VHF185P
Connected seq. 
>VHF185P.Seq
ATATTTTTAAAAAATGGCAACAATTGAAGATACTTNAAAGATAATAACAATCCAATTTTA
GAACGTAAAAAAGCTCCAAATAGATTATTTGTAGAAGAAGCAATTAATGATGATAATTCA
GTAGTTACATTAAATCCAGAGACAATGGATCAATTACAATTCTTTAGAGGTGACACACTT
TTAATTAAAGGTAAAAAGAGAAGAGATACCGTTTGTATTGTATTGTCAGATCCAACCATC
GATCCATCAAAGATCCGTATGAATAAAGTTGTAAGAAATAATCTTCGTGTTAGATTAGGT
GACATGATTTCAGTTCACCAATGTTCAGATGTTAAATATGGCAAACGTATTCATGTGTTA
CCAATTGATGATACCATAGAAGGTCTCAGTGGCAATCTCTTTGATCTTTACCTCAAACCA
TACTTTTTAGAAGCCTACCGTCCAGTCCGTAAGGGTGACTTATTTTTAGTACGTGGTGGT
ATGAGAGCCGTAGAATTTAAAGTTGTCGAAACCGACCCAGGTGAATATTGTATCGTTGCA
CCAGAGACATTTATACATTGTGAAGGTGAGGCAGTCAAGAGAGA----------TATNAT
CGATCCAGCTATCNTCCGTCCAGGTNGTTTAGATCAACTCATTTACATTCCATTACCAGA
TTTACCATTTNGTGTTGCCATTTTCAAGGNTTGTTTCAATAAATCNCCAGTNGCCAAGGA
TGTAGATTTAGAATTTTTGGGTCAAAAGANTCAAGGTTTTTTTGGTGCAGATTTAACTGA
AATTTGTCAACGTGCCTGTAAATTAGCTANTNGTGAATCCATTGAAAAGGATATTGAATC
CNCCAAAGCCCGTCAAGAATTTGGTGATACCAANATGGAANANGAATNTGTCGATCCAGT
ACCAGAAATTAATNGTGATCNCTTCCAANAAGCCATGANATNTGNTNGTCGTTNTGTATC
CGATAATGATANTAGAAAATATGAAAGCTTTGCTCAAACTTTAGTTCAATCAAGAGGTCT
TGGTAANAATTTCAAATTCCCAGATCAAGAATCTTCAGGTC
Length of connected seq. 1051
Full length Seq ID -
Full length Seq. -
Length of full length seq. -