>VHF176 (VHF176Q) /CSM/VH/VHF1-D/VHF176Q.Seq.d/
XXXXXXXXXXTTAGTCCAAATCAATCAGAGAGAGGTTTTTCATTGGCTTTGGAACATGGC
ATTGGTGGTGCACGTCTTTCTCACAATCATCGTAAACAATATAACTATGTATATCAATCA
CTCTCCTTGTGGCAACATATTCTTCATGATATGTTTAAACTTTGGTACTTTGCAGAAATT
GATCTTTTATGCGGACACAGATATCAATTATCAAACACTGGTCAAGGTNTAAATCGTATT
CAACAATGTCCAAACGTTGGTAAAATTATGCATAGTATTNTACGTCAAACTCAAAAAAAA
GTTGGTGATGATTGGGTTGGTTCTTCCGTTATTCATTTGGGTGATCATAACGTTCCAAAN
TCTTTGGTTTTCATCGATAAATACACTCAAATNTCTAGAATCTTAAGTCCAATCGTTTTA
ACCATTGAATATATTCCAAAGATCACCGATCCAAATTTAGTTGCTTACATCAAAAACACC
TTTGGTGGTCCACAAACTTTAACCAAAATCATCCTTTGTGATTTCTTTAAACACGCTTTC
GATGGTAGTGGTGCCGATAACTTTTTCGATGCTGGCTCTTGTATTGATGGTAGATTAACT
TCTGCTTGGCAATGGTCTGCTCAAATCGCAAAGAAACC

---SPNQSERGFSLALEHGIGGARLSHNHRKQYNYVYQSLSLWQHILHDMFKLWYFAEID
LLCGHRYQLSNTGQGXNRIQQCPNVGKIMHSIXRQTQKKVGDDWVGSSVIHLGDHNVPXS
LVFIDKYTQXSRILSPIVLTIEYIPKITDPNLVAYIKNTFGGPQTLTKIILCDFFKHAFD
GSGADNFFDAGSCIDGRLTSAWQWSAQIAKK

Frame A:
---*sksirerffigfgtwhwwctsfsqss*ti*lcisitllvatyss*yv*tlvlcrn*
sfmrtqisiikhwsrxksystmskrw*nya*yxtsnskksw**lgwffrysfg*s*rskx
fgfhr*ihsnx*nlksnrfnh*iyskdhrskfsclhqkhlwwstnfnqnhpl*fl*trfr
w*wcr*lfrcwlly*w*infclamvcsnrket


Frame B:
---SPNQSERGFSLALEHGIGGARLSHNHRKQYNYVYQSLSLWQHILHDMFKLWYFAEID
LLCGHRYQLSNTGQGXNRIQQCPNVGKIMHSIXRQTQKKVGDDWVGSSVIHLGDHNVPXS
LVFIDKYTQXSRILSPIVLTIEYIPKITDPNLVAYIKNTFGGPQTLTKIILCDFFKHAFD
GSGADNFFDAGSCIDGRLTSAWQWSAQIAKK


Frame C:
---vqinqrevfhwlwnmalvvhvfltiivnnitmyinhspcgniffmiclnfgtlqkli
fyadtdinyqtlvkv*ivfnnvqtlvklcivxyvklkkklvmiglvlplfiwviitfqxl
wfssintlkxles*vqsf*plnifqrspiqi*lltsktplvvhkl*pkssfvislntlsm
vvvpitfsmlalvlmvd*lllgngllksqrn

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF176 (VHF176Q) /CSM/VH/VHF1-D/VHF176Q.Seq.d/                       1174   0.0
CHQ406 (CHQ406Q) /CSM/CH/CHQ4-A/CHQ406Q.Seq.d/                       1168   0.0
VHP208 (VHP208Q) /CSM/VH/VHP2-A/VHP208Q.Seq.d/                       1166   0.0
VHO745 (VHO745Q) /CSM/VH/VHO7-B/VHO745Q.Seq.d/                       1166   0.0
VHK760 (VHK760Q) /CSM/VH/VHK7-C/VHK760Q.Seq.d/                       1166   0.0
VHI553 (VHI553Q) /CSM/VH/VHI5-C/VHI553Q.Seq.d/                       1166   0.0
VHI541 (VHI541Q) /CSM/VH/VHI5-B/VHI541Q.Seq.d/                       1166   0.0
VHE160 (VHE160Q) /CSM/VH/VHE1-C/VHE160Q.Seq.d/                       1166   0.0
VHD241 (VHD241Q) /CSM/VH/VHD2-B/VHD241Q.Seq.d/                       1166   0.0
VHC810 (VHC810Q) /CSM/VH/VHC8-A/VHC810Q.Seq.d/                       1166   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

EH431674|EH431674.1 NPE00000755 Neocallimastix patriciarum ZAP II cDNA library Neocallimastix patriciarum cDNA, mRNA sequence.    50   2e-12  4
EC805743|EC805743.1 SAE00000979 ir_esapbrog Sphaeroforma arctica cDNA, mRNA sequence.    66   1e-06  1
ES282011|ES282011.1 PP034C10.XT7 non-sporulating culture of P. brassicae Phytophthora brassicae cDNA, mRNA sequence.    58   3e-04  1
ES278287|ES278287.1 PP032A10.XT7 non-sporulating culture of P. brassicae Phytophthora brassicae cDNA, mRNA sequence.    58   3e-04  1
AC021798|AC021798.5 Homo sapiens clone RP11-25K3, WORKING DRAFT SEQUENCE, 17 unordered pieces.    48   0.33   1
AC099065|AC099065.2 Homo sapiens chromosome 1 clone RP11-105I12, complete sequence.    48   0.33   1
AC096542|AC096542.2 Homo sapiens chromosome 1 clone RP11-332L18, complete sequence.    48   0.33   1
AC130532|AC130532.3 Mus musculus BAC clone RP23-189G24 from 7, complete sequence.    42   0.60   2
AC157249|AC157249.2 Dasypus novemcinctus clone VMRC5-110M7, WORKING DRAFT SEQUENCE.    46   1.3    1
CR382125|CR382125.1 Kluyveromyces lactis strain NRRL Y-1140 chromosome E of strain NRRL  Y-1140 of Kluyveromyces lactis.    46   1.3    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP001575_110(CP001575|pid:none) Micromonas sp. RCC299 chromosome...   258   1e-67
CR382131_1131(CR382131|pid:none) Yarrowia lipolytica strain CLIB...   245   9e-64
CR940353_528(CR940353|pid:none) Theileria annulata strain Ankara...   239   5e-62
CP001142_478(CP001142|pid:none) Phaeodactylum tricornutum CCAP 1...   216   6e-55
AM910996_269(AM910996|pid:none) Plasmodium knowlesi strain H chr...   210   2e-53
AL844507_105(AL844507|pid:none) Plasmodium falciparum 3D7 chromo...   210   3e-53
CR380956_239(CR380956|pid:none) Candida glabrata strain CBS138 c...   201   1e-50
CR382125_995(CR382125|pid:none) Kluyveromyces lactis strain NRRL...   198   1e-49
AE016819_283(AE016819|pid:none) Ashbya gossypii (= Eremothecium ...   196   6e-49
CU928179_226(CU928179|pid:none) Zygosaccharomyces rouxii strain ...   196   6e-49

 psg: 0.77  gvh: 0.51  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VHF176 is cyt

>VHF176Z.Seq
NNNNNNNNNNTTAGTCCAAATCAATCAGAGAGAGGTTTTTCATTGGCTTTGGAACATGGC
ATTGGTGGTGCACGTCTTTCTCACAATCATCGTAAACAATATAACTATGTATATCAATCA
CTCTCCTTGTGGCAACATATTCTTCATGATATGTTTAAACTTTGGTACTTTGCAGAAATT
GATCTTTTATGCGGACACAGATATCAATTATCAAACACTGGTCAAGGTNTAAATCGTATT
CAACAATGTCCAAACGTTGGTAAAATTATGCATAGTATTNTACGTCAAACTCAAAAAAAA
GTTGGTGATGATTGGGTTGGTTCTTCCGTTATTCATTTGGGTGATCATAACGTTCCAAAN
TCTTTGGTTTTCATCGATAAATACACTCAAATNTCTAGAATCTTAAGTCCAATCGTTTTA
ACCATTGAATATATTCCAAAGATCACCGATCCAAATTTAGTTGCTTACATCAAAAACACC
TTTGGTGGTCCACAAACTTTAACCAAAATCATCCTTTGTGATTTCTTTAAACACGCTTTC
GATGGTAGTGGTGCCGATAACTTTTTCGATGCTGGCTCTTGTATTGATGGTAGATTAACT
TCTGCTTGGCAATGGTCTGCTCAAATCGCAAAGAAACC