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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF158
Library VH
(Link to library)
Clone ID VHF158
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHF158P
(Link to Original site)
Representative DNA sequence 
>VHF158 (VHF158Q) /CSM/VH/VHF1-C/VHF158Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAAXX
XXXXXXXXCGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGT
AACGGTGCCAATNTCNTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAA
GANTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAAC
ATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGA
GGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCC
TCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATT
GGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGT
ATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATCNTTCGGTTTC
ACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCAT
TGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACC
AGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTC
TAAACAATTTAAGACGCACTTNAGTTTTCAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVG---

---EWDKNDAMNIWSFGPEGNGANXXVNVTKGVQYLNEIKXSFVGAFQWATKEGVVCDEN
MRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPXILEPIYLVEITAPENAI
GGIXSVLNRRRGIVIGEERRIGSPLLSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDH
WASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*dalxfs
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllv---

---rmg*k*chehlvfrsrr*rcqxxrqchqrcsife*n*rxfrwcfpmghqrrccl**k
herypfqsl*chpph*chpqrwwsnhpncssctlcc*inclpxylrtnllsrnycprkry
wwylxctqss*rycyw*rtsyrfpiiicqssftsirixrfhr*ftfphcrssfptmclrs
lgfnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnlrrtxvf

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*---

---EWDKNDAMNIWSFGPEGNGANXXVNVTKGVQYLNEIKXSFVGAFQWATKEGVVCDEN
MRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPXILEPIYLVEITAPENAI
GGIXSVLNRRRGIVIGEERRIGSPLLSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDH
WASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*dalxfs

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVG---

---ngikmmp*tfglsvqkvtvpxsssmspkvfni*mklkxlslvlsngppkkvlfvmkt
*evsvsifmmspstlmpstevvvkssqllvvysmlln*lppqxs*nqst**kllpqktll
vvfxlysivvevlllvknvvsvphyylskliyqy*nxsvspliyvptlpvklshnvssii
glqlvlstkirkplksl*pperekv*pqksqiltnstknskqfkthxsfq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF158 (VHF158Q) /CSM/VH/VHF1-C/VHF158Q.Seq.d/                       2418   0.0
VHF681 (VHF681Q) /CSM/VH/VHF6-D/VHF681Q.Seq.d/                       1296   0.0
VHE779 (VHE779Q) /CSM/VH/VHE7-D/VHE779Q.Seq.d/                       1296   0.0
VHQ491 (VHQ491Q) /CSM/VH/VHQ4-D/VHQ491Q.Seq.d/                       1289   0.0
VHQ483 (VHQ483Q) /CSM/VH/VHQ4-D/VHQ483Q.Seq.d/                       1289   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                       1289   0.0
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                       1289   0.0
VHQ453 (VHQ453Q) /CSM/VH/VHQ4-C/VHQ453Q.Seq.d/                       1289   0.0
VHQ434 (VHQ434Q) /CSM/VH/VHQ4-B/VHQ434Q.Seq.d/                       1289   0.0
VHQ410 (VHQ410Q) /CSM/VH/VHQ4-A/VHQ410Q.Seq.d/                       1289   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.  1183   0.0    2
AF107291|AF107291.1 Candida parapsilosis elongation factor 2 (EFT2) gene, partial cds.    72   3e-68  13
AF107286|AF107286.1 Candida albicans elongation factor 2 (EFT2) gene, partial cds.    78   5e-66  9
Y09664|Y09664.1 C.albicans EFT2 gene.                              78   1e-64  9
AF107289|AF107289.1 Candida tropicalis elongation factor 2 (EFT2) gene, partial cds.    64   3e-63  10
AF534908|AF534908.2 Tetrahymena thermophila elongation factor 2 gene, complete cds.    84   2e-45  6
AF107290|AF107290.1 Candida lusitaniae elongation factor 2 (EFT2) gene, partial cds.    64   3e-45  7
AY144999|AY144999.1 Saccharomyces kluyveri clone Contig1706 EFT2 gene, complete cds.    78   5e-44  7
EH015431|EH015431.1 USDA-FP_188032 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt081_G08 5', mRNA sequence.    78   2e-42  4
EH012247|EH012247.1 USDA-FP_185167 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt044_H07 5', mRNA sequence.    78   3e-42  4
dna update 2007.10.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   273   8e-72
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   273   1e-71
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   270   6e-71
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   270   8e-71
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   270   8e-71
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   269   1e-70
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   269   2e-70
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   268   3e-70
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   268   3e-70
AK298498_1(AK298498|pid:none) Homo sapiens cDNA FLJ56548 complet...   268   3e-70
protein update 2009. 7. 2
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHF158 is cyt
5' end seq. ID VHF158F
5' end seq. 
>VHF158F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAANN
NNNNNNNN
Length of 5' end seq. 608
3' end seq. ID VHF158Z
3' end seq. 
>VHF158Z.Seq
NNNNNNNNNNCGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAG
GTAACGGTGCCAATNTCNTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTA
AAGANTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAA
ACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACA
GAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTG
CCTCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTA
TTGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTC
GTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATCNTTCGGTT
TCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATC
ATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCA
CCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAAC
TCTAAACAATTTAAGACGCACTTNAGTTTTCAA
Length of 3' end seq. 693
Connected seq. ID VHF158P
Connected seq. 
>VHF158P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAA--
--------CGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGT
AACGGTGCCAATNTCNTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAA
GANTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAAC
ATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGA
GGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCC
TCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATT
GGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGT
ATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATCNTTCGGTTTC
ACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCAT
TGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACC
AGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTC
TAAACAATTTAAGACGCACTTNAGTTTTCAA
Length of connected seq. 1281
Full length Seq ID -
Full length Seq. -
Length of full length seq. -