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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF146
Library VH
(Link to library)
Clone ID VHF146
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12300-1
Original site URL
Representative seq. ID VHF146P
(Link to Original site)
Representative DNA sequence 
>VHF146 (VHF146Q) /CSM/VH/VHF1-B/VHF146Q.Seq.d/
AAAAATTAAAATTTGAATTAATTATCTTGATCAATCAAAAATCAGATTCAATTTATAATG
AACAAAAAGAATATCAAAAACAAGAACGTCTTTTAAGAGAAGATGATAGAAAATATATAG
AAACTTTAAAGATTTCAATTTTCTCATCATTAAAAGAGGCAAAGATTGAAAGAGATCAAT
TATCCAATCAAATCAATTCATTATTACAAGATAATAAAACCATTCAAATCAATGCTGAAC
AAGCTGAACAATTGGCAAGCACAAAGAATGATTAACTATTGAAAGCAAATGATGAATTGT
TGGCACAATTGGAACAATCTAGAAAACAACGTTTATTTGAAATTCAAGAATCAGAGAATA
GATTCAAACAAGATAGAGAACAATTAATCAATGATCATAAACAACAAGAGAAATTAGAAC
AACAAAAGAGAGATCTCGAAGAGACTAGAATCATTGAACAATTGAAACAAGAGTCGGAAC
AACGTAAAAAACAATATGAAGATCATTTGAAAGAAAGAGAATCAGAGAGAGAGAAAGAGG
TTCAACAATTATTATTAAAACAAAAAGAAGCTGAACAATTAATTGAACAACAXXXXXXXX
XXAAAGATGATGAAGTATTTGCTCAAGCAAGAAAAGGTGNTAAATCTTCTTTCAATAAAG
AAAAAGATGATCATAGTAATAATAACNACNCCTCAACACNATCATCATCATCATCATCGT
CATCATCAGCTGATAGATCAAAATCNCCATTAACAGTTGTTGTTAATGATGATAAATNTG
AATNTGAAACTGAATCAACNCCATCGATTCCATCAAAAGCAAATTTAAAACCAGTTTCAA
AACGNTCAGGNTACNCCAACAAGTGATATAGATAGTACAAATAGTGAATCGGAATCAGTG
GTTAAATCAACCACCAAAAAACCAAGTGCTGGTATAAACGCTGGTGGCAATGATAAACCA
GTATCAA
sequence update 2002.10.25
Translated Amino Acid sequence 
klkfeliilinqksdsiyneqkeyqkqerllreddrkyietlkisifsslkeakierdql
snqinsllqdnktiqinaeqaeqlastknd*LLKANDELLAQLEQSRKQRLFEIQESENR
FKQDREQLINDHKQQEKLEQQKRDLEETRIIEQLKQESEQRKKQYEDHLKERESEREKEV
QQLLLKQKEAEQLIEQ---

---KDDEVFAQARKGXKSSFNKEKDDHSNNNXXSTXSSSSSSSSSADRSKSPLTVVVNDD
KXEXETESTPSIPSKANLKPVSKRSGYXNK*yr*yk**igisg*inhqktkcwykrwwq*
*tsi
Translated Amino Acid sequence (All Frames) 
Frame A:
kn*nln*ls*siknqiqfimnkkniknknvf*ekmieni*kl*rfqfshh*krqrlkein
ypiksihyykiikpfksmlnklnnwqaqrminy*kqmmncwhnwnnlennvylkfknqri
dsnkienn*smiinnkrn*nnkreiskrleslnn*nksrnnvknnmkii*kkenqrerkr
fnnyy*nkkklnn*lnn---

---KDDEVFAQARKGXKSSFNKEKDDHSNNNXXSTXSSSSSSSSSADRSKSPLTVVVNDD
KXEXETESTPSIPSKANLKPVSKRSGYXNK*yr*yk**igisg*inhqktkcwykrwwq*
*tsi

Frame B:
kiki*inyldqskirfnl**tkrisktrtsfkrr**kiyrnfkdfnfliikrgkd*krsi
iqsnqfiitr**nhsnqc*ts*tigkhke*ltiesk**ivgtigti*kttfi*nsrire*
iqtr*rtinq*s*ttreirttkersrrd*nh*tietrvgtt*kti*rsferkrirererg
stiiiktkrs*tin*tt---

---kmmkyllkqekvxnllsikkkmiiviittpqhxhhhhhrhhqlidqnxh*qlllmmi
nxnxklnqxhrfhqkqi*nqfqnxqxtptsdidstnsesesvvksttkkpsaginaggnd
kpvs

Frame C:
klkfeliilinqksdsiyneqkeyqkqerllreddrkyietlkisifsslkeakierdql
snqinsllqdnktiqinaeqaeqlastknd*LLKANDELLAQLEQSRKQRLFEIQESENR
FKQDREQLINDHKQQEKLEQQKRDLEETRIIEQLKQESEQRKKQYEDHLKERESEREKEV
QQLLLKQKEAEQLIEQ---

---r**sicsskkrx*iffq*rkr*s****xxlntiiiiiiviis**ikixinscc****
x*x*n*inxidsikskfktsfktxrxxqqvi*ivqivnrnqwlnqppknqvlv*tlvami
nqyq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF146 (VHF146Q) /CSM/VH/VHF1-B/VHF146Q.Seq.d/                       1007   0.0
VHC876 (VHC876Q) /CSM/VH/VHC8-D/VHC876Q.Seq.d/                        991   0.0
VHM360 (VHM360Q) /CSM/VH/VHM3-C/VHM360Q.Seq.d/                        961   0.0
VHO368 (VHO368Q) /CSM/VH/VHO3-C/VHO368Q.Seq.d/                        844   0.0
VHD202 (VHD202Q) /CSM/VH/VHD2-A/VHD202Q.Seq.d/                        492   e-138
VHK525 (VHK525Q) /CSM/VH/VHK5-B/VHK525Q.Seq.d/                        416   e-115
VFO565 (VFO565Q) /CSM/VF/VFO5-C/VFO565Q.Seq.d/                        416   e-115
VFD839 (VFD839Q) /CSM/VF/VFD8-B/VFD839Q.Seq.d/                        416   e-115
VHF859 (VHF859Q) /CSM/VH/VHF8-C/VHF859Q.Seq.d/                        410   e-113
VFD824 (VFD824Q) /CSM/VF/VFD8-A/VFD824Q.Seq.d/                        398   e-110
own update 2002.12.26
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CP000123|CP000123.1 Mycoplasma capricolum subsp. capricolum ATCC 27343, complete genome.    40   0.008  14
AC003970|AC003970.1 Arabidopsis thaliana chromosome I BAC F14J9 genomic sequence contains phyA marker, complete sequence.    38   0.080  2
BD193425|BD193425.1 Enterococcus faecialis polynucleotides and polypeptides.    50   0.13   1
AR396074|AR396074.1 Sequence 2089 from patent US 6617156.          50   0.13   1
AE016830|AE016830.1 Enterococcus faecalis V583, complete genome.    50   0.13   1
AC183761|AC183761.3 Pan troglodytes BAC clone CH251-710C5 from chromosome 7, complete sequence.    50   0.13   1
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    38   0.18   11
BX293980|BX293980.2 Mycoplasma mycoides subsp. mycoides SC str. PG1, complete genome.    38   0.26   2
EJ562043|EJ562043.1 1092959666558 Global-Ocean-Sampling_GS-29-01-01-1P0-1P3KB marine metagenome genomic clone 1061001857664 5', genomic survey sequence.    34   0.37   2
EH012322|EH012322.1 USDA-FP_185234 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt045_F10 5', mRNA sequence.    48   0.51   1
dna update 2007.10.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY042084_3(AY042084|pid:none) Plasmodium falciparum erythrocyte ...    63   2e-08
BC151676_1(BC151676|pid:none) Bos taurus trichohyalin, mRNA (cDN...    62   3e-08
U93872_83(U93872|pid:none) Kaposi's sarcoma-associated herpesvir...    61   7e-08
AF360120_1(AF360120|pid:none) Human herpesvirus 8 ORF73 gene, co...    61   7e-08
AF148805_82(AF148805|pid:none) Human herpesvirus 8 type P isolat...    60   9e-08
(P37709) RecName: Full=Trichohyalin;  &CS673546_1(CS673546|pid:n...    60   9e-08
AF305694_1(AF305694|pid:none) Kaposi's sarcoma-associated herpes...    59   2e-07
(Q86KL1) RecName: Full=Probable E3 ubiquitin-protein ligase bre1...    59   3e-07
AF192756_1(AF192756|pid:none) Kaposi's sarcoma-associated herpes...    58   4e-07
BC091989_1(BC091989|pid:none) Danio rerio similar to GTPase, IMA...    58   6e-07
protein update 2009. 7. 2
PSORT 

 psg: 0.75  gvh: 0.29  alm: 0.62  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 16.0 %: cytoskeletal
	 12.0 %: mitochondrial

>> prediction for VHF146 is nuc
5' end seq. ID VHF146F
5' end seq. 
>VHF146F.Seq
AAAAATTAAAATTTGAATTAATTATCTTGATCAATCAAAAATCAGATTCAATTTATAATG
AACAAAAAGAATATCAAAAACAAGAACGTCTTTTAAGAGAAGATGATAGAAAATATATAG
AAACTTTAAAGATTTCAATTTTCTCATCATTAAAAGAGGCAAAGATTGAAAGAGATCAAT
TATCCAATCAAATCAATTCATTATTACAAGATAATAAAACCATTCAAATCAATGCTGAAC
AAGCTGAACAATTGGCAAGCACAAAGAATGATTAACTATTGAAAGCAAATGATGAATTGT
TGGCACAATTGGAACAATCTAGAAAACAACGTTTATTTGAAATTCAAGAATCAGAGAATA
GATTCAAACAAGATAGAGAACAATTAATCAATGATCATAAACAACAAGAGAAATTAGAAC
AACAAAAGAGAGATCTCGAAGAGACTAGAATCATTGAACAATTGAAACAAGAGTCGGAAC
AACGTAAAAAACAATATGAAGATCATTTGAAAGAAAGAGAATCAGAGAGAGAGAAAGAGG
TTCAACAATTATTATTAAAACAAAAAGAAGCTGAACAATTAATTGAACAACANNNNNNNN
NN
Length of 5' end seq. 602
3' end seq. ID VHF146Z
3' end seq. 
>VHF146Z.Seq
NNNNNNNNNNAAAGATGATGAAGTATTTGCTCAAGCAAGAAAAGGTGNTAAATCTTCTTT
CAATAAAGAAAAAGATGATCATAGTAATAATAACNACNCCTCAACACNATCATCATCATC
ATCATCGTCATCATCAGCTGATAGATCAAAATCNCCATTAACAGTTGTTGTTAATGATGA
TAAATNTGAATNTGAAACTGAATCAACNCCATCGATTCCATCAAAAGCAAATTTAAAACC
AGTTTCAAAACGNTCAGGNTACNCCAACAAGTGATATAGATAGTACAAATAGTGAATCGG
AATCAGTGGTTAAATCAACCACCAAAAAACCAAGTGCTGGTATAAACGCTGGTGGCAATG
ATAAACCAGTATCAA
Length of 3' end seq. 375
Connected seq. ID VHF146P
Connected seq. 
>VHF146P.Seq
AAAAATTAAAATTTGAATTAATTATCTTGATCAATCAAAAATCAGATTCAATTTATAATG
AACAAAAAGAATATCAAAAACAAGAACGTCTTTTAAGAGAAGATGATAGAAAATATATAG
AAACTTTAAAGATTTCAATTTTCTCATCATTAAAAGAGGCAAAGATTGAAAGAGATCAAT
TATCCAATCAAATCAATTCATTATTACAAGATAATAAAACCATTCAAATCAATGCTGAAC
AAGCTGAACAATTGGCAAGCACAAAGAATGATTAACTATTGAAAGCAAATGATGAATTGT
TGGCACAATTGGAACAATCTAGAAAACAACGTTTATTTGAAATTCAAGAATCAGAGAATA
GATTCAAACAAGATAGAGAACAATTAATCAATGATCATAAACAACAAGAGAAATTAGAAC
AACAAAAGAGAGATCTCGAAGAGACTAGAATCATTGAACAATTGAAACAAGAGTCGGAAC
AACGTAAAAAACAATATGAAGATCATTTGAAAGAAAGAGAATCAGAGAGAGAGAAAGAGG
TTCAACAATTATTATTAAAACAAAAAGAAGCTGAACAATTAATTGAACAACA--------
--AAAGATGATGAAGTATTTGCTCAAGCAAGAAAAGGTGNTAAATCTTCTTTCAATAAAG
AAAAAGATGATCATAGTAATAATAACNACNCCTCAACACNATCATCATCATCATCATCGT
CATCATCAGCTGATAGATCAAAATCNCCATTAACAGTTGTTGTTAATGATGATAAATNTG
AATNTGAAACTGAATCAACNCCATCGATTCCATCAAAAGCAAATTTAAAACCAGTTTCAA
AACGNTCAGGNTACNCCAACAAGTGATATAGATAGTACAAATAGTGAATCGGAATCAGTG
GTTAAATCAACCACCAAAAAACCAAGTGCTGGTATAAACGCTGGTGGCAATGATAAACCA
GTATCAA
Length of connected seq. 957
Full length Seq ID -
Full length Seq. -
Length of full length seq. -