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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF145
Library VH
(Link to library)
Clone ID VHF145
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15430-1
Original site URL
Representative seq. ID VHF145E
(Link to Original site)
Representative DNA sequence 
>VHF145 (VHF145Q) /CSM/VH/VHF1-B/VHF145Q.Seq.d/
GAGTGTGTCCATCGAGAGAACTTACGTATAGAAGAAAATAAAAGAAATGCCATCCCAAAG
CAATAATGTTATTACATCTTCTACAGCCTCATCATCAATGAGTTCGTCATCCAATAGTTC
TGATGCATCATCAACATCATCGTCAAACACCAACAATGCACATTCCTCCAACAACCAATT
TGTGAGTGGCCAATTAAATTTAGTTGGAAATACTCACACAAAAATTAATGATAATTATGA
AATTATTTCAAAAATAGGTGAAGGTATTTCAGGATCTGTATTTAAAGCAATTAAAAAAGG
TACAGAAGAAATGGTTGCACTTAAAAATTTTAAAGGTTGGACTGAAGGTGATAGAGCCTC
AAAGGAAGAATGTTCATTATTACAACAATTAAGACATATTCCATATATTACACCAGTCAT
CGATATCTATACAAATTTTGAAACCTCAGAGTATATCATTGTATTTCCATACTTTGAACA
TGATCTCTCTGGTCTTTTAAGTGAACATAGACTATCAATTCCACAAGTTAAATGTTATTT
CAAACAATTATTAGAGGGTATAAATGAAATTCATAATGCTGGTGTTATGCACAGAGATAT
TAAAGCTGCAAATTTACTTGTAAATAATAAAGGATCATTATTTATTGGTGATTTAGGTAC
AGCAACATCATATACAAAAAGATCAGTATTTTCAAGTAAAGTTGTAACATTATGGTACAG
AGCACCAGAATTATTATTGGGATCAACTCAATATGGACCAGAAATTGATATGTGGTCAAT
TGGTTGTGTTTTAATTGAATTAGTTACTTCACGTAACTTTTTACCAGGTAGTTCTGAACA
ACAACAGCTCGAGGCAATTTGTAAATTATGTGGTACACCAACTGATGAAATTTGGCCAAA
CGTTTCTCAATTACAAAACTTTAATCTAATTTCACATTTACCAGTTTATCCAAGTAGATT
AAGAACTGTTTTTAAAAACTTTTCAAATGATTTTATAGAATTATTAGAAGGTTTATTAAC
ATTAAATCCAAAGAAAAGANTAA
sequence update 2002.10.27
Translated Amino Acid sequence 
svsiertyv*KKIKEMPSQSNNVITSSTASSSMSSSSNSSDASSTSSSNTNNAHSSNNQF
VSGQLNLVGNTHTKINDNYEIISKIGEGISGSVFKAIKKGTEEMVALKNFKGWTEGDRAS
KEECSLLQQLRHIPYITPVIDIYTNFETSEYIIVFPYFEHDLSGLLSEHRLSIPQVKCYF
KQLLEGINEIHNAGVMHRDIKAANLLVNNKGSLFIGDLGTATSYTKRSVFSSKVVTLWYR
APELLLGSTQYGPEIDMWSIGCVLIELVTSRNFLPGSSEQQQLEAICKLCGTPTDEIWPN
VSQLQNFNLISHLPVYPSRLRTVFKNFSNDFIELLEGLLTLNPKKRX
Translated Amino Acid sequence (All Frames) 
Frame A:
ecvhrenlrieenkrnaipkq*cyyifysliinefviq*f*ciiniivkhqqctflqqpi
cewpikfswkyshkn***l*nyfknr*ryfrici*sn*kryrrngct*kf*rld*r**sl
kgrmfiittiktysiyytshrylykf*nlrvyhcisil*t*slwsfk*t*tinsts*mlf
qtiirgyk*ns*cwcyaqry*sckftck**riiiyw*frysniiykkisifk*scnimvq
striiiginsiwtrn*yvvnwlcfn*isyft*lftr*f*tttargnl*imwytn**nlak
rfsitkl*snftftslsk*ikncf*klfk*fyriirrfinikskekx


Frame B:
svsiertyv*KKIKEMPSQSNNVITSSTASSSMSSSSNSSDASSTSSSNTNNAHSSNNQF
VSGQLNLVGNTHTKINDNYEIISKIGEGISGSVFKAIKKGTEEMVALKNFKGWTEGDRAS
KEECSLLQQLRHIPYITPVIDIYTNFETSEYIIVFPYFEHDLSGLLSEHRLSIPQVKCYF
KQLLEGINEIHNAGVMHRDIKAANLLVNNKGSLFIGDLGTATSYTKRSVFSSKVVTLWYR
APELLLGSTQYGPEIDMWSIGCVLIELVTSRNFLPGSSEQQQLEAICKLCGTPTDEIWPN
VSQLQNFNLISHLPVYPSRLRTVFKNFSNDFIELLEGLLTLNPKKRX


Frame C:
vcpsreltyrrk*kkchpkaimllhllqphhq*vrhpivlmhhqhhrqtptmhippttnl
*van*i*leiltqklmiimklfqk*vkvfqdlylkqlkkvqkkwlhlkilkvglkviepq
rknvhyynn*difhilhqssisiqilkpqsislyfhtlnmislvf*vnidyqfhklnvis
nny*rv*mkfimlvlcteilklqiyl*iikdhyllvi*vqqhhiqkdqyfqvkl*hygte
hqnyywdqlnmdqklicgqlvvf*ln*llhvtfyqvvlnnnssrqfvnyvvhqlmkfgqt
flnyktli*fhiyqfiqvd*elflktfqmil*ny*kvy*h*iqrkx*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF145 (VHF145Q) /CSM/VH/VHF1-B/VHF145Q.Seq.d/                       2062   0.0
AFG131 (AFG131Q) /CSM/AF/AFG1-B/AFG131Q.Seq.d/                       2016   0.0
AFA604 (AFA604Q) /CSM/AF/AFA6-A/AFA604Q.Seq.d/                       1320   0.0
VFG669 (VFG669Q) /CSM/VF/VFG6-C/VFG669Q.Seq.d/                       1243   0.0
AHA866 (AHA866Q) /CSM/AH/AHA8-C/AHA866Q.Seq.d/                       1243   0.0
AHM293 (AHM293Q) /CSM/AH/AHM2-D/AHM293Q.Seq.d/                       1231   0.0
VHG492 (VHG492Q) /CSM/VH/VHG4-D/VHG492Q.Seq.d/                       1229   0.0
VHQ474 (VHQ474Q) /CSM/VH/VHQ4-D/VHQ474Q.Seq.d/                       1209   0.0
AFI694 (AFI694Q) /CSM/AF/AFI6-D/AFI694Q.Seq.d/                       1164   0.0
AHJ593 (AHJ593Q) /CSM/AH/AHJ5-D/AHJ593Q.Seq.d/                       1148   0.0
own update 2002.12.26
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    72   3e-12  2
EC818966|EC818966.1 SME00008568 esmbsro2 Sawyeria marylandensis cDNA, mRNA sequence.    52   2e-10  2
BM276045|BM276045.1 PfESToaa69e08.y1 Plasmodium falciparum 3D7 gametocyte cDNA library Plasmodium falciparum 3D7 cDNA 5' similar to TR:P90584 P90584 MO15-RELATED PROTEIN KINASE PFMRK ;, mRNA sequence.    76   2e-09  1
U73195|U73195.1 Plasmodium falciparum MO15-related protein kinase Pfmrk (Pfmrk) gene, complete cds.    76   2e-09  1
AF179420|AF179420.1 Plasmodium falciparum isolate FCC1/HN MO15-related protein kinase Pfmrk gene, complete cds.    76   2e-09  1
AE014831|AE014831.1 Plasmodium falciparum 3D7 chromosome 10 section 3 of 7 of the complete sequence.    76   2e-09  1
AJ010592|AJ010592.2 Guillardia theta DNA for complete sequence of nucleomorph chromosome 2.    48   2e-07  2
ES451909| PREDICTED: similar to PISSLRE  - Monodelphis domestica.  Score = 392 bits (1007), Expect = e-108, mRNA sequence.    66   2e-06  1
EC093665| cyclin-dependent kinase 10 isoform 1 - Mus musculus. Score = 381 bits (978), Expect = e-104, mRNA sequence.    66   2e-06  1
AW342354|AW342354.1 GthEST211 Guillardia theta Lambda Zap II cDNA Library Guillardia theta cDNA clone Est42_49_30.T, mRNA sequence.    42   1e-05  2
dna update 2007.10.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54Y06) RecName: Full=Probable cyclin-dependent serine/threonin...   552   e-156
CR954201_420(CR954201|pid:none) Ostreococcus tauri strain OTTH05...   209   1e-52
AY675095_1(AY675095|pid:none) Ostreococcus tauri cell cycle depe...   209   1e-52
CP000581_439(CP000581|pid:none) Ostreococcus lucimarinus CCE9901...   207   4e-52
AJ428950_1(AJ428950|pid:none) Physcomitrella patens mRNA for put...   200   6e-50
BT046183_1(BT046183|pid:none) Arabidopsis thaliana unknown prote...   199   2e-49
U83118_1(U83118|pid:none) Arabidopsis thaliana cdc2-like protein...   199   2e-49
AF120153_1(AF120153|pid:none) Arabidopsis thaliana cyclin-depend...   199   2e-49
AC010164_11(AC010164|pid:none) Arabidopsis thaliana chromosome I...   198   3e-49
AP008209_1466(AP008209|pid:none) Oryza sativa (japonica cultivar...   196   9e-49
protein update 2009. 7.11
PSORT 

 psg: 0.87  gvh: 0.52  alm: 0.42  top: 0.30  tms: 0.00  mit: 0.41  mip: 0.00
 nuc: 0.03  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 24.0 %: extracellular, including cell wall
	 20.0 %: cytoplasmic
	 20.0 %: mitochondrial
	 16.0 %: nuclear
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoskeletal

>> prediction for VHF145 is exc
5' end seq. ID VHF145F
5' end seq. 
>VHF145F.Seq
GAGTGTGTCCATCGAGAGAACTTACGTATAGAAGAAAATAAAAGAAATGCCATCCCAAAG
CAATAATGTTATTACATCTTCTACAGCCTCATCATCAATGAGTTCGTCATCCAATAGTTC
TGATGCATCATCAACATCATCGTCAAACACCAACAATGCACATTCCTCCAACAACCAATT
TGTGAGTGGCCAATTAAATTTAGTTGGAAATACTCACACAAAAATTAATGATAATTATGA
AATTATTTCAAAAATAGGTGAAGGTATTTCAGGATCTGTATTTAAAGCAATTAAAAAAGG
TACAGAAGAAATGGTTGCACTTAAAAATTTTAAAGGTTGGACTGAAGGTGATAGAGCCTC
AAAGGAAGAATGTTCATTATTACAACAATTAAGACATATTCCATATATTACACCAGTCAT
CGATATCTATACAAATTTTGAAACCTCAGAGTATATCATTGTATTTCCATACTTTGAACA
TGATCTCTCTGGTCTTTTAAGTGAACATAGACTATCAATTCCACAAGTTAAATGTTATTT
CAAACAATTATTAGAGGGTATAAATGAAATTCATAATGCTGGTGTTATGCACAGAGATAT
TAAAGCTGCAAATTTACTTGTAAATNNNNNNNNNN
Length of 5' end seq. 635
3' end seq. ID VHF145Z
3' end seq. 
>VHF145Z.Seq
NNNNNNNNNNTGTTATTTCAAACAATTATTAGAGGGTATAAATGAAATTCATAATGCTGG
TGTTATGCACAGAGATATTAAAGCTGCAAATTTACTTGTAAATAATAAAGGATCATTATT
TATTGGTGATTTAGGTACAGCAACATCATATACAAAAAGATCAGTATTTTCAAGTAAAGT
TGTAACATTATGGTACAGAGCACCAGAATTATTATTGGGATCAACTCAATATGGACCAGA
AATTGATATGTGGTCAATTGGTTGTGTTTTAATTGAATTAGTTACTTCACGTAACTTTTT
ACCAGGTAGTTCTGAACAACAACAGCTCGAGGCAATTTGTAAATTATGTGGTACACCAAC
TGATGAAATTTGGCCAAACGTTTCTCAATTACAAAACTTTAATCTAATTTCACATTTACC
AGTTTATCCAAGTAGATTAAGAACTGTTTTTAAAAACTTTTCAAATGATTTTATAGAATT
ATTAGAAGGTTTATTAACATTAAATCCAAAGAAAAGANTAA
Length of 3' end seq. 521
Connected seq. ID VHF145P
Connected seq. 
>VHF145P.Seq
GAGTGTGTCCATCGAGAGAACTTACGTATAGAAGAAAATAAAAGAAATGCCATCCCAAAG
CAATAATGTTATTACATCTTCTACAGCCTCATCATCAATGAGTTCGTCATCCAATAGTTC
TGATGCATCATCAACATCATCGTCAAACACCAACAATGCACATTCCTCCAACAACCAATT
TGTGAGTGGCCAATTAAATTTAGTTGGAAATACTCACACAAAAATTAATGATAATTATGA
AATTATTTCAAAAATAGGTGAAGGTATTTCAGGATCTGTATTTAAAGCAATTAAAAAAGG
TACAGAAGAAATGGTTGCACTTAAAAATTTTAAAGGTTGGACTGAAGGTGATAGAGCCTC
AAAGGAAGAATGTTCATTATTACAACAATTAAGACATATTCCATATATTACACCAGTCAT
CGATATCTATACAAATTTTGAAACCTCAGAGTATATCATTGTATTTCCATACTTTGAACA
TGATCTCTCTGGTCTTTTAAGTGAACATAGACTATCAATTCCACAAGTTAAATGTTATTT
CAAACAATTATTAGAGGGTATAAATGAAATTCATAATGCTGGTGTTATGCACAGAGATAT
TAAAGCTGCAAATTTACTTGTAAAT----------TGTTATTTCAAACAATTATTAGAGG
GTATAAATGAAATTCATAATGCTGGTGTTATGCACAGAGATATTAAAGCTGCAAATTTAC
TTGTAAATAATAAAGGATCATTATTTATTGGTGATTTAGGTACAGCAACATCATATACAA
AAAGATCAGTATTTTCAAGTAAAGTTGTAACATTATGGTACAGAGCACCAGAATTATTAT
TGGGATCAACTCAATATGGACCAGAAATTGATATGTGGTCAATTGGTTGTGTTTTAATTG
AATTAGTTACTTCACGTAACTTTTTACCAGGTAGTTCTGAACAACAACAGCTCGAGGCAA
TTTGTAAATTATGTGGTACACCAACTGATGAAATTTGGCCAAACGTTTCTCAATTACAAA
ACTTTAATCTAATTTCACATTTACCAGTTTATCCAAGTAGATTAAGAACTGTTTTTAAAA
ACTTTTCAAATGATTTTATAGAATTATTAGAAGGTTTATTAACATTAAATCCAAAGAAAA
GANTAA
Length of connected seq. 1136
Full length Seq ID VHF145E
Full length Seq. 
>VHF145E.Seq
GAGTGTGTCCATCGAGAGAACTTACGTATAGAAGAAAATAAAAGAAATGCCATCCCAAAG
CAATAATGTTATTACATCTTCTACAGCCTCATCATCAATGAGTTCGTCATCCAATAGTTC
TGATGCATCATCAACATCATCGTCAAACACCAACAATGCACATTCCTCCAACAACCAATT
TGTGAGTGGCCAATTAAATTTAGTTGGAAATACTCACACAAAAATTAATGATAATTATGA
AATTATTTCAAAAATAGGTGAAGGTATTTCAGGATCTGTATTTAAAGCAATTAAAAAAGG
TACAGAAGAAATGGTTGCACTTAAAAATTTTAAAGGTTGGACTGAAGGTGATAGAGCCTC
AAAGGAAGAATGTTCATTATTACAACAATTAAGACATATTCCATATATTACACCAGTCAT
CGATATCTATACAAATTTTGAAACCTCAGAGTATATCATTGTATTTCCATACTTTGAACA
TGATCTCTCTGGTCTTTTAAGTGAACATAGACTATCAATTCCACAAGTTAAATGTTATTT
CAAACAATTATTAGAGGGTATAAATGAAATTCATAATGCTGGTGTTATGCACAGAGATAT
TAAAGCTGCAAATTTACTTGTAAATAATAAAGGATCATTATTTATTGGTGATTTAGGTAC
AGCAACATCATATACAAAAAGATCAGTATTTTCAAGTAAAGTTGTAACATTATGGTACAG
AGCACCAGAATTATTATTGGGATCAACTCAATATGGACCAGAAATTGATATGTGGTCAAT
TGGTTGTGTTTTAATTGAATTAGTTACTTCACGTAACTTTTTACCAGGTAGTTCTGAACA
ACAACAGCTCGAGGCAATTTGTAAATTATGTGGTACACCAACTGATGAAATTTGGCCAAA
CGTTTCTCAATTACAAAACTTTAATCTAATTTCACATTTACCAGTTTATCCAAGTAGATT
AAGAACTGTTTTTAAAAACTTTTCAAATGATTTTATAGAATTATTAGAAGGTTTATTAAC
ATTAAATCCAAAGAAAAGANTAA
Length of full length seq. 1043