>VHF140 (VHF140Q) /CSM/VH/VHF1-B/VHF140Q.Seq.d/
XXXXXXXXXXCCGATAATCNTTGTAACATTGATGTTGTTTCTCCAAAATGGTATTCTTCA
CTTTATTGGTGTGATAATATTAATAAGATGGGAACTTGCAAAAAATGTTCAAACACTGGA
GTACTTCNTTGTTTATGGTTTGATCATCAAGTAGTTACTATCAAAGAAACNCTTCAACTC
AATCAATTACCAGGCACTGATAAAAAAACNATAGGTCAAAGAACAAAGAGTGAAATATTT
AATGAGAGGGAGTTGTTAGTTACAAAAATGGGAGAAAGAAAAATACAATTCCAAGAAGAA
TATGATACTATTAAGACTGCAATTGTTGACTTTTCAGTATTCTTGTGGCACCATTCATTA
ATTAAATTTAATCGTTACTATGAAGATTATATCAACATGCAAATAAAGCAATTGGAAAAT
TCTGAAAACTCGCAAGAATTAATTCAAAAACTCAATGAACACTTAAGTGAATATAAAGCA
AGTGTTGAAGAAATAAAACATAATGTCCAAAATAATAA

---DNXCNIDVVSPKWYSSLYWCDNINKMGTCKKCSNTGVLXCLWFDHQVVTIKETLQLN
QLPGTDKKTIGQRTKSEIFNERELLVTKMGERKIQFQEEYDTIKTAIVDFSVFLWHHSLI
KFNRYYEDYINMQIKQLENSENSQELIQKLNEHLSEYKASVEEIKHNVQNN

Frame A:
---r*sl*h*ccfskmvfftllv**y**dgnlqkmfkhwstslfmv*ssssyyqrnxstq
sitrh**knxrsknke*ni**egvvsykngrkkntiprri*yy*dcnc*lfsilvapfin
*i*sll*rlyqhankaigkf*klarinsktq*tlk*i*skc*rnkt*cpk**


Frame B:
---DNXCNIDVVSPKWYSSLYWCDNINKMGTCKKCSNTGVLXCLWFDHQVVTIKETLQLN
QLPGTDKKTIGQRTKSEIFNERELLVTKMGERKIQFQEEYDTIKTAIVDFSVFLWHHSLI
KFNRYYEDYINMQIKQLENSENSQELIQKLNEHLSEYKASVEEIKHNVQNN


Frame C:
---iixvtlmlflqngilhfigviilirwelaknvqtleyfxvygliik*llskkxfnsi
nyqalikkx*vkeqrvkylmrgsc*lqkwekekynskknmillrlqlltfqyscgtih*l
nlivtmkiistck*snwkilktrkn*fknsmnt*vnikqvlkk*nimskii

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF140 (VHF140Q) /CSM/VH/VHF1-B/VHF140Q.Seq.d/                        942   0.0
SFC514 (SFC514Q) /CSM/SF/SFC5-A/SFC514Q.Seq.d/                        934   0.0
AHC883 (AHC883Q) /CSM/AH/AHC8-D/AHC883Q.Seq.d/                        934   0.0
AFM784 (AFM784Q) /CSM/AF/AFM7-D/AFM784Q.Seq.d/                        934   0.0
AFJ260 (AFJ260Q) /CSM/AF/AFJ2-C/AFJ260Q.Seq.d/                        934   0.0
VHI440 (VHI440Q) /CSM/VH/VHI4-B/VHI440Q.Seq.d/                        920   0.0
CHM834 (CHM834Q) /CSM/CH/CHM8-B/CHM834Q.Seq.d/                        894   0.0
AHK709 (AHK709Q) /CSM/AH/AHK7-A/AHK709Q.Seq.d/                        825   0.0
AHJ342 (AHJ342Q) /CSM/AH/AHJ3-B/AHJ342Q.Seq.d/                        823   0.0
AHC420 (AHC420Q) /CSM/AH/AHC4-A/AHC420Q.Seq.d/                        823   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CU062636|CU062636.1 Pig DNA sequence *** SEQUENCING IN PROGRESS *** from clone  CH242-106P14.    52   0.017  1
EB976324|EB976324.1 15497600 ZF40 Danio rerio cDNA clone 4214392, mRNA sequence.    50   0.068  1
CT009687|CT009687.10 Zebrafish DNA sequence from clone DKEY-5I20 in linkage group 14.    50   0.068  1
AC196888|AC196888.2 Myotis lucifugus clone CH235-68J20, WORKING DRAFT SEQUENCE, 3 ordered pieces.    48   0.25   4
DU674504|DU674504.1 Bst007046 lambda03 Boechera stricta genomic clone SAD-EX-09-T3_J24, genomic survey sequence.    48   0.27   1
EW478106|EW478106.1 rova027_e10.y1 ova Sus scrofa cDNA 5', mRNA sequence.    46   1.1    1
BV254101|BV254101.1 S235P6186FE7.T0 ItalianGreyhound Canis familiaris STS genomic, sequence tagged site.    46   1.1    1
AC148146|AC148146.3 Canis familiaris clone RP81-205M18, WORKING DRAFT SEQUENCE, 12 ordered pieces.    46   1.1    1
AC165491|AC165491.2 Bos taurus clone CH240-172J24, *** SEQUENCING IN PROGRESS ***, 27 unordered pieces.    46   1.1    1
EK237592|EK237592.1 1095460208673 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061005672542 5', genomic survey sequence.    46   1.1    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB120174_1(AB120174|pid:none) Dictyostelium discoideum DD8-14 mR...    72   6e-12
AC114263_3(AC114263|pid:none) Dictyostelium discoideum chromosom...    60   4e-08
CP000575_1221(CP000575|pid:none) Staphylothermus marinus F1, com...    37   0.38
CP000728_3239(CP000728|pid:none) Clostridium botulinum F str. La...    37   0.38
AM910989_22(AM910989|pid:none) Plasmodium knowlesi strain H chro...    36   0.65
AM412317_3267(AM412317|pid:none) Clostridium botulinum A str. AT...    36   0.65
AY811138_1(AY811138|pid:none) Schistosoma japonicum SJCHGC00387 ...    35   1.1
AJ416696_1(AJ416696|pid:none) Plasmodium falciparum mRNA for put...    35   1.1
(Q0VC16) RecName: Full=Melanoma inhibitory activity protein 3; A...    35   1.5
CP001279_992(CP001279|pid:none) Nautilia profundicola AmH, compl...    35   1.5

 psg: 0.75  gvh: 0.57  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.14  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 20.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: Golgi

>> prediction for VHF140 is cyt

>VHF140Z.Seq
NNNNNNNNNNCCGATAATCNTTGTAACATTGATGTTGTTTCTCCAAAATGGTATTCTTCA
CTTTATTGGTGTGATAATATTAATAAGATGGGAACTTGCAAAAAATGTTCAAACACTGGA
GTACTTCNTTGTTTATGGTTTGATCATCAAGTAGTTACTATCAAAGAAACNCTTCAACTC
AATCAATTACCAGGCACTGATAAAAAAACNATAGGTCAAAGAACAAAGAGTGAAATATTT
AATGAGAGGGAGTTGTTAGTTACAAAAATGGGAGAAAGAAAAATACAATTCCAAGAAGAA
TATGATACTATTAAGACTGCAATTGTTGACTTTTCAGTATTCTTGTGGCACCATTCATTA
ATTAAATTTAATCGTTACTATGAAGATTATATCAACATGCAAATAAAGCAATTGGAAAAT
TCTGAAAACTCGCAAGAATTAATTCAAAAACTCAATGAACACTTAAGTGAATATAAAGCA
AGTGTTGAAGAAATAAAACATAATGTCCAAAATAATAA