>VHF138 (VHF138Q) /CSM/VH/VHF1-B/VHF138Q.Seq.d/
AATTTTCATCCAAGTCGTATAGTTTCATAATACATATAATATGGATTTTCTTAAATCGCT
ACCACCTTTACAAACAACAGATAGTAATTCGACAACAGCAGCAGTAAATACAACAACAAC
AACAGCGACAAATACAGCAACAACAACAGCGACAAATACAGCAACAACAACAACAACAAC
AACAACAACAAATTTAAATAATTTAATAAATGAAAGTAAAAAAAAGATTCATAGATTAAC
TCAAGAAGTTGTAAATAAAATATCAGCAGGTGAAGTTATACAAAGACCAAGTAATGCATT
AAAAGAATTATTAGAGAATTGTTTAGATGCAAAATCAACAACTATTACAGTTACAGTTAA
AGATGGTGGTATGAAATTCTTACAAATTCAAGACAATGGTTCTGGTATTAGATTGGAGGA
TATGGGCATTGTTTGTGAACGTTTCACCACAAGTAAATTAACTAAATTTGAAGATCTAAG
AAGTATTCAATCATTTGGATTTCGTGGTGAAGCATTATCAAGTATAAGTCACGTTTCTCA
CTTGAAAATTCTAACTAAAACCGCTGATTCACCATGTGCCTATCGTGCATGTTATCTTAA
TGGTAAXXXXXXXXXX

ifiqvv*FHNTYNMDFLKSLPPLQTTDSNSTTAAVNTTTTTATNTATTTATNTATTTTTT
TTTNLNNLINESKKKIHRLTQEVVNKISAGEVIQRPSNALKELLENCLDAKSTTITVTVK
DGGMKFLQIQDNGSGIRLEDMGIVCERFTTSKLTKFEDLRSIQSFGFRGEALSSISHVSH
LKILTKTADSPCAYRACYLNG---

Frame A:
nfhpsrivs*yi*ygfs*iattftnnr**fdnssskynnnnsdkysnnnsdkysnnnnnn
nnnkfk*fnk*k*kkds*insrsck*nisr*sytktk*cikriirelfrckinnyysys*
rwwyeiltnsrqwfwy*iggyghcl*tfhhk*in*i*rskkysiiwisw*siikyksrfs
lensn*nr*ftmclscmls*w---


Frame B:
ifiqvv*FHNTYNMDFLKSLPPLQTTDSNSTTAAVNTTTTTATNTATTTATNTATTTTTT
TTTNLNNLINESKKKIHRLTQEVVNKISAGEVIQRPSNALKELLENCLDAKSTTITVTVK
DGGMKFLQIQDNGSGIRLEDMGIVCERFTTSKLTKFEDLRSIQSFGFRGEALSSISHVSH
LKILTKTADSPCAYRACYLNG---


Frame C:
fssksysfiihiiwiflnryhlykqqivirqqqq*iqqqqqrqiqqqqqrqiqqqqqqqq
qqqi*ii**mkvkkrfid*lkkl*ikyqqvklykdqvmh*kny*riv*mqnqqllqlqlk
mvv*nsykfktmvlvldwriwalfvnvspqvn*lnlki*evfnhldfvvkhyqv*vtflt
*kf*lkplihhvpivhvilmv---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHO552 (VHO552Q) /CSM/VH/VHO5-C/VHO552Q.Seq.d/                        775   0.0
VHM109 (VHM109Q) /CSM/VH/VHM1-A/VHM109Q.Seq.d/                        775   0.0
VHH396 (VHH396Q) /CSM/VH/VHH3-D/VHH396Q.Seq.d/                        775   0.0
VHF138 (VHF138Q) /CSM/VH/VHF1-B/VHF138Q.Seq.d/                        775   0.0
AHM822 (AHM822Q) /CSM/AH/AHM8-A/AHM822Q.Seq.d/                        775   0.0
AHQ659 (AHQ659Q) /CSM/AH/AHQ6-C/AHQ659Q.Seq.d/                        769   0.0
AHE577 (AHE577Q) /CSM/AH/AHE5-D/AHE577Q.Seq.d/                        472   e-132
SHL335 (SHL335Q) /CSM/SH/SHL3-B/SHL335Q.Seq.d/                         40   0.039
SHJ804 (SHJ804Q) /CSM/SH/SHJ8-A/SHJ804Q.Seq.d/                         40   0.039
SHH476 (SHH476Q) /CSM/SH/SHH4-D/SHH476Q.Seq.d/                         40   0.039

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

DC222808|DC222808.1 Plasmodium berghei strain ANKA cDNA clone:MZ006449, erythrocytic schizont 5'-EST.    80   7e-15  2
DB734512|DB734512.1 Apis mellifera head cDNA, RIKEN full-length enriched library, clone:BH10023B20, 5' end partial sequence.    46   5e-14  4
DC233859|DC233859.1 Plasmodium berghei strain ANKA cDNA clone:SG028885, salivary grand sporozoite 5'-EST.    80   9e-11  1
BG604094|BG604094.1 EST503184 Plasmodium yoelii sporozoite cDNA Plasmodium yoelii cDNA clone PYCDX86, mRNA sequence.    76   1e-09  1
DT616036| DNA mismatc, mRNA sequence.    54   6e-09  3
DT832480|DT832480.1 LB00416.CR_K13 GC_BGC-04 Bos taurus cDNA clone IMAGE:8033511 5', mRNA sequence.    42   2e-06  3
DV868803|DV868803.1 LB0251.CR_H07 GC_BGC-25 Bos taurus cDNA clone IMAGE:8278329 5', mRNA sequence.    42   2e-06  3
EH179261|EH179261.1 LB01749.CR_L09 GC_BGC-17 Bos taurus cDNA clone IMAGE:8568539 5', mRNA sequence.    42   2e-06  3
DY495485|DY495485.1 sh2P0016C01_F.ab1 adult sheep fracture callus 7d Ovis aries cDNA, mRNA sequence.    42   2e-06  3
EE817744|EE817744.1 010914ONDC179088HT ONDC Ovis aries cDNA, mRNA sequence.    42   2e-06  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54KD8) RecName: Full=DNA mismatch repair protein Mlh1; AltName...   284   1e-75
CR760439_1(CR760439|pid:none) Xenopus tropicalis finished cDNA, ...   189   4e-47
AK312609_1(AK312609|pid:none) Homo sapiens cDNA, FLJ92988, highl...   185   8e-46
(P40692) RecName: Full=DNA mismatch repair protein Mlh1; AltName...   185   8e-46
U17857_1(U17857|pid:none) Human mismatch repair (hMLH1) gene, ex...   185   8e-46
BC021815_1(BC021815|pid:none) Mus musculus mutL homolog 1 (E. co...   185   1e-45
AK038265_1(AK038265|pid:none) Mus musculus 16 days neonate thymu...   185   1e-45
U60872_1(U60872|pid:none) Mus musculus MLH1 gene, exon 5 and par...   185   1e-45
(Q9JK91) RecName: Full=DNA mismatch repair protein Mlh1; AltName...   185   1e-45
AK168849_1(AK168849|pid:none) Mus musculus 17 days embryo heart ...   185   1e-45

 psg: 0.80  gvh: 0.45  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.12  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHF138 is nuc

>VHF138F.Seq
AATTTTCATCCAAGTCGTATAGTTTCATAATACATATAATATGGATTTTCTTAAATCGCT
ACCACCTTTACAAACAACAGATAGTAATTCGACAACAGCAGCAGTAAATACAACAACAAC
AACAGCGACAAATACAGCAACAACAACAGCGACAAATACAGCAACAACAACAACAACAAC
AACAACAACAAATTTAAATAATTTAATAAATGAAAGTAAAAAAAAGATTCATAGATTAAC
TCAAGAAGTTGTAAATAAAATATCAGCAGGTGAAGTTATACAAAGACCAAGTAATGCATT
AAAAGAATTATTAGAGAATTGTTTAGATGCAAAATCAACAACTATTACAGTTACAGTTAA
AGATGGTGGTATGAAATTCTTACAAATTCAAGACAATGGTTCTGGTATTAGATTGGAGGA
TATGGGCATTGTTTGTGAACGTTTCACCACAAGTAAATTAACTAAATTTGAAGATCTAAG
AAGTATTCAATCATTTGGATTTCGTGGTGAAGCATTATCAAGTATAAGTCACGTTTCTCA
CTTGAAAATTCTAACTAAAACCGCTGATTCACCATGTGCCTATCGTGCATGTTATCTTAA
TGGTAANNNNNNNNNN