>VHF132 (VHF132Q) /CSM/VH/VHF1-B/VHF132Q.Seq.d/
AATTATTTAATTTTCATTTTTTTTTAATTTATTAAAAATAAATATAGATAAATTTTTTTA
AAGAATTAGTCATTTTATGCCAATTGGAAATAACAAAGATAACGATGGAACTACCCTGAA
TATCTCAGGGTTAACCATCTCTGATAAGAGTTCGTCAACTTCATCAAACAACAACACAAC
AACAACAACAACACAAACTAGTGAACCATATGTCCCACCATCTCGTAGAAACAAGATGAA
TAATAGTTCAGGTTCATCAAGAGACGATTATCCATCACCATCTGACCGTGATAATCGTGA
TTCACCATTCAATCGTGACCGTCGTGACGATGGTTACAGAGGTGGTAACAATTTTAGGGA
AAATAACTCTAATAACAACAACAACAGTAGAGATAACTACAAAAGTCCATCATTCTCAAG
AAACAATAATAGTAATGGTGGTGGTAGTAGTAGTGGTAGCGGTGGTTGGGATAATGGTCC
AAGAGAACAAGGCCCACCAAGAGGTAATAGTTACTCTTATTCACCAAATAATTCATTCTC
TCGTGGTGGTAATCAAGGTGGTTATGGTAATAGAGGCGGAAGTGGCAGTAGTTTTGGTAA
AXXXXXXXXXXCTTGTGATANTCTTACNTACTTTTTAAATCAAAGAAATTTCCCAACTAC
NTGTATTCATGGTGATTTAACTCAACCAGAAAGAGAAAATGCACTTCAATCTTTCCGNTC
TTTTGCAACTCCATTCTTAGTAGCAACTGATATTGCATCAAGAGGTCTCCACATCGGTAA
TGTTAATTTGGTTATCAATTTCGACTTGCCTACCGACATACATATCTACGTTCATCGTAT
TGGTAGAACCGGAAGAGCAGGAAAAAAAGGTTTAGCAATTTCNTTCTTCAATGAAAAGAA
TAAACCAGTTGGTGCTGAACTTTTGAAATTAATGAAAGCTTCAAATCAAGATATTCCAGA
TTGGTTTGAAAAGATGGTACACAATCTCCGTATGTATAAAGGTCCATCAAACTCAAAATC
AAATAGTCCATTCAACAAATCTTACAATAGTCATCACAATAGAGATGATAACCGTGGTGG
TTATGGTGGTGGTGGTGGTGGTAATGGAGGTTACAGTAACAACNGTAATGATAGAAGAGA
AGATAAATCAGATTACTCAATGCATCACCCATACTTTAGCAACAATGGTGGTTCATATAA
TTAAACATTCACNTCATGTCATTCGTTTAATAAAAAATAATAAATCCATTTTAATTCA

ii*fsfffnllkinidkff*RISHFMPIGNNKDNDGTTLNISGLTISDKSSSTSSNNNTT
TTTTQTSEPYVPPSRRNKMNNSSGSSRDDYPSPSDRDNRDSPFNRDRRDDGYRGGNNFRE
NNSNNNNNSRDNYKSPSFSRNNNSNGGGSSSGSGGWDNGPREQGPPRGNSYSYSPNNSFS
RGGNQGGYGNRGGSGSSFGK---

---CDXLTYFLNQRNFPTTCIHGDLTQPERENALQSFRSFATPFLVATDIASRGLHIGNV
NLVINFDLPTDIHIYVHRIGRTGRAGKKGLAISFFNEKNKPVGAELLKLMKASNQDIPDW
FEKMVHNLRMYKGPSNSKSNSPFNKSYNSHHNRDDNRGGYGGGGGGNGGYSNNXNDRRED
KSDYSMHHPYFSNNGGSYN*tftschsfnkk**ihfns

Frame A:
nylififf*fiknkyr*iflkn*sfyanwk*qr*rwnypeylrvnhl**efvnfikqqhn
nnnntn**ticptis*kqde**frfikrrlsiti*p**s*ftiqs*ps*rwlqrw*qf*g
k*l**qqqq*r*lqksiilkkq***www***w*rwlg*wskrtrptkr**lllftk*fil
sww*srwlw**rrkwq*fw*---

---lvixlxtf*ikeisqlxvfmvi*lnqkekmhfnlsxllqlhs**qlilhqevstsvm
liwlsistclptyistfivlvepeeqekkv*qfxssmkrinqlvlnf*n**klqikifqi
glkrwytisvcikvhqtqnqivhstnltivitiemitvvvmvvvvvvmevtvttvmieek
inqitqcithtlatmvvhiikhsxhvirliknnksili

Frame B:
ii*fsfffnllkinidkff*RISHFMPIGNNKDNDGTTLNISGLTISDKSSSTSSNNNTT
TTTTQTSEPYVPPSRRNKMNNSSGSSRDDYPSPSDRDNRDSPFNRDRRDDGYRGGNNFRE
NNSNNNNNSRDNYKSPSFSRNNNSNGGGSSSGSGGWDNGPREQGPPRGNSYSYSPNNSFS
RGGNQGGYGNRGGSGSSFGK---

---l*xsyxlfkskkfpnyxysw*fnstrkrkctsifpxfcnsilssn*ycikrsphr*c
*fgyqfrlayrhtylrssyw*nrksrkkrfsnfxlq*ke*tswc*tfeinesfksrysrl
v*kdgtqspyv*rsiklkik*siqqilq*ssq*r**pwwlwwwwww*wrlq*qx***krr
*irllnaspil*qqwwfi*lnihxmsfv**kiinpf*f

Frame C:
lfnfhffliy*k*i*inffkelvilcqleitkitmelp*isqg*pslirvrqlhqtttqq
qqqhklvnhmshhlvetr*iivqvhqetiihhhltviivihhsivtvvtmvtevvtilgk
itlittttveittkvhhsqetiivmvvvvvvvavvgimvqenkahqevivtlihqiihsl
vvvikvvmvieaevavvlv---

---CDXLTYFLNQRNFPTTCIHGDLTQPERENALQSFRSFATPFLVATDIASRGLHIGNV
NLVINFDLPTDIHIYVHRIGRTGRAGKKGLAISFFNEKNKPVGAELLKLMKASNQDIPDW
FEKMVHNLRMYKGPSNSKSNSPFNKSYNSHHNRDDNRGGYGGGGGGNGGYSNNXNDRRED
KSDYSMHHPYFSNNGGSYN*tftschsfnkk**ihfns

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF132 (VHF132Q) /CSM/VH/VHF1-B/VHF132Q.Seq.d/                       1604   0.0
VHQ404 (VHQ404Q) /CSM/VH/VHQ4-A/VHQ404Q.Seq.d/                        837   0.0
VHP734 (VHP734Q) /CSM/VH/VHP7-B/VHP734Q.Seq.d/                        837   0.0
VHP681 (VHP681Q) /CSM/VH/VHP6-D/VHP681Q.Seq.d/                        837   0.0
VHP588 (VHP588Q) /CSM/VH/VHP5-D/VHP588Q.Seq.d/                        837   0.0
VHP118 (VHP118Q) /CSM/VH/VHP1-A/VHP118Q.Seq.d/                        837   0.0
VHO596 (VHO596Q) /CSM/VH/VHO5-D/VHO596Q.Seq.d/                        837   0.0
VHO585 (VHO585Q) /CSM/VH/VHO5-D/VHO585Q.Seq.d/                        837   0.0
VHN546 (VHN546Q) /CSM/VH/VHN5-B/VHN546Q.Seq.d/                        837   0.0
VHN331 (VHN331Q) /CSM/VH/VHN3-B/VHN331Q.Seq.d/                        837   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL731714|AL731714.9 Mouse DNA sequence from clone RP23-117H24 on chromosome 11 Contains  the gene for a novel protein (4933427E13Rik).    48   2e-08  3
EC761979|EC761979.1 PSE00006532 rw_mgpallid Polysphondylium pallidum cDNA, mRNA sequence.    52   2e-07  2
AC175904|AC175904.1 Strongylocentrotus purpuratus clone R3-3043G20, WORKING DRAFT SEQUENCE, 25 unordered pieces.    46   5e-04  2
AY160096|AY160096.1 Dictyostelium discoideum nucleotide exchange factor RasGEF G (gefG) gene, complete cds.    32   0.002  6
DX871512|DX871512.1 ZMMBLf0001F18.f ZMMBLf Zea mays genomic clone ZMMBLf0001F18 5', genomic survey sequence.    46   0.002  2
AC117147|AC117147.4 Rattus norvegicus clone CH230-398H18, WORKING DRAFT SEQUENCE, 1 ordered piece.    56   0.003  1
BX248512|BX248512.14 Zebrafish DNA sequence from clone CH211-284D12 in linkage group 2.    30   0.006  3
AL929352|AL929352.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 2/4.    38   0.007  2
AC206753|AC206753.1 Zea mays chromosome 2 clone ZMMBBb-566G2; ZMMBBb0566G02, *** SEQUENCING IN PROGRESS ***, 19 unordered pieces.    44   0.007  2
BQ576817|BQ576817.1 PfESToab10b04.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:YAJE_SCHPO Q09911 HYPOTHETICAL 59.0 KDA PROTEIN C30D11.14C IN CHROMOSOME I. [1] ;, mRNA sequence.    38   0.009  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54QS3) RecName: Full=Probable ATP-dependent RNA helicase ddx3;...   301   2e-80
(Q6Z4K6) RecName: Full=DEAD-box ATP-dependent RNA helicase 52B; ...   147   1e-33
BT085991_1(BT085991|pid:none) Zea mays full-length cDNA clone ZM...   143   1e-32
(Q75HJ0) RecName: Full=DEAD-box ATP-dependent RNA helicase 37;  ...   142   2e-32
FN357514_12(FN357514|pid:none) Schistosoma mansoni genome sequen...   142   2e-32
EU953399_1(EU953399|pid:none) Zea mays clone 1406878 unknown mRNA.    142   3e-32
AM048814_1(AM048814|pid:none) Platynereis dumerilii mRNA for PL1...   141   4e-32
(Q2R1M8) RecName: Full=DEAD-box ATP-dependent RNA helicase 52C; ...   140   1e-31
AM444101_1(AM444101|pid:none) Vitis vinifera contig VV78X103110....   140   1e-31
CP001333_316(CP001333|pid:none) Micromonas sp. RCC299 chromosome...   139   3e-31

 psg: 0.88  gvh: 0.39  alm: 0.46  top: 0.30  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.11  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 44.0 %: nuclear
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHF132 is cyt

>VHF132F.Seq
AATTATTTAATTTTCATTTTTTTTTAATTTATTAAAAATAAATATAGATAAATTTTTTTA
AAGAATTAGTCATTTTATGCCAATTGGAAATAACAAAGATAACGATGGAACTACCCTGAA
TATCTCAGGGTTAACCATCTCTGATAAGAGTTCGTCAACTTCATCAAACAACAACACAAC
AACAACAACAACACAAACTAGTGAACCATATGTCCCACCATCTCGTAGAAACAAGATGAA
TAATAGTTCAGGTTCATCAAGAGACGATTATCCATCACCATCTGACCGTGATAATCGTGA
TTCACCATTCAATCGTGACCGTCGTGACGATGGTTACAGAGGTGGTAACAATTTTAGGGA
AAATAACTCTAATAACAACAACAACAGTAGAGATAACTACAAAAGTCCATCATTCTCAAG
AAACAATAATAGTAATGGTGGTGGTAGTAGTAGTGGTAGCGGTGGTTGGGATAATGGTCC
AAGAGAACAAGGCCCACCAAGAGGTAATAGTTACTCTTATTCACCAAATAATTCATTCTC
TCGTGGTGGTAATCAAGGTGGTTATGGTAATAGAGGCGGAAGTGGCAGTAGTTTTGGTAA
ANNNNNNNNNN

>VHF132Z.Seq
NNNNNNNNNNCTTGTGATANTCTTACNTACTTTTTAAATCAAAGAAATTTCCCAACTACN
TGTATTCATGGTGATTTAACTCAACCAGAAAGAGAAAATGCACTTCAATCTTTCCGNTCT
TTTGCAACTCCATTCTTAGTAGCAACTGATATTGCATCAAGAGGTCTCCACATCGGTAAT
GTTAATTTGGTTATCAATTTCGACTTGCCTACCGACATACATATCTACGTTCATCGTATT
GGTAGAACCGGAAGAGCAGGAAAAAAAGGTTTAGCAATTTCNTTCTTCAATGAAAAGAAT
AAACCAGTTGGTGCTGAACTTTTGAAATTAATGAAAGCTTCAAATCAAGATATTCCAGAT
TGGTTTGAAAAGATGGTACACAATCTCCGTATGTATAAAGGTCCATCAAACTCAAAATCA
AATAGTCCATTCAACAAATCTTACAATAGTCATCACAATAGAGATGATAACCGTGGTGGT
TATGGTGGTGGTGGTGGTGGTAATGGAGGTTACAGTAACAACNGTAATGATAGAAGAGAA
GATAAATCAGATTACTCAATGCATCACCCATACTTTAGCAACAATGGTGGTTCATATAAT
TAAACATTCACNTCATGTCATTCGTTTAATAAAAAATAATAAATCCATTTTAATTCA

>VHF132P.Seq
AATTATTTAATTTTCATTTTTTTTTAATTTATTAAAAATAAATATAGATAAATTTTTTTA
AAGAATTAGTCATTTTATGCCAATTGGAAATAACAAAGATAACGATGGAACTACCCTGAA
TATCTCAGGGTTAACCATCTCTGATAAGAGTTCGTCAACTTCATCAAACAACAACACAAC
AACAACAACAACACAAACTAGTGAACCATATGTCCCACCATCTCGTAGAAACAAGATGAA
TAATAGTTCAGGTTCATCAAGAGACGATTATCCATCACCATCTGACCGTGATAATCGTGA
TTCACCATTCAATCGTGACCGTCGTGACGATGGTTACAGAGGTGGTAACAATTTTAGGGA
AAATAACTCTAATAACAACAACAACAGTAGAGATAACTACAAAAGTCCATCATTCTCAAG
AAACAATAATAGTAATGGTGGTGGTAGTAGTAGTGGTAGCGGTGGTTGGGATAATGGTCC
AAGAGAACAAGGCCCACCAAGAGGTAATAGTTACTCTTATTCACCAAATAATTCATTCTC
TCGTGGTGGTAATCAAGGTGGTTATGGTAATAGAGGCGGAAGTGGCAGTAGTTTTGGTAA
A----------CTTGTGATANTCTTACNTACTTTTTAAATCAAAGAAATTTCCCAACTAC
NTGTATTCATGGTGATTTAACTCAACCAGAAAGAGAAAATGCACTTCAATCTTTCCGNTC
TTTTGCAACTCCATTCTTAGTAGCAACTGATATTGCATCAAGAGGTCTCCACATCGGTAA
TGTTAATTTGGTTATCAATTTCGACTTGCCTACCGACATACATATCTACGTTCATCGTAT
TGGTAGAACCGGAAGAGCAGGAAAAAAAGGTTTAGCAATTTCNTTCTTCAATGAAAAGAA
TAAACCAGTTGGTGCTGAACTTTTGAAATTAATGAAAGCTTCAAATCAAGATATTCCAGA
TTGGTTTGAAAAGATGGTACACAATCTCCGTATGTATAAAGGTCCATCAAACTCAAAATC
AAATAGTCCATTCAACAAATCTTACAATAGTCATCACAATAGAGATGATAACCGTGGTGG
TTATGGTGGTGGTGGTGGTGGTAATGGAGGTTACAGTAACAACNGTAATGATAGAAGAGA
AGATAAATCAGATTACTCAATGCATCACCCATACTTTAGCAACAATGGTGGTTCATATAA
TTAAACATTCACNTCATGTCATTCGTTTAATAAAAAATAATAAATCCATTTTAATTCA