>VHF123 (VHF123Q) /CSM/VH/VHF1-A/VHF123Q.Seq.d/
AATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTC
AAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGC
AACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAG
AAGAAGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAA
TTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAA
TTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAGTTTTAAAAG
AAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAAT
CATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTAT
CAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGA
TCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAAXXXXXXXXXXCAATTGG
GTGTCTCNGAGACTGGTTTACCTTTTTTAGATCNTTTTAGACTTTTAATCACTCATATTG
GTAATGCTATGGGTTATATTAGATTGGTCAGATCCGGTGGTTTACACTATTGTAGTAATG
CTATCAAATTCGTACCAGACTTGAAAAAGATTCCAAAATTCCAAGATTTAACTTCAAAAG
ATGCCTTCTCACTTGAAACNATTCAAGCTAGTACCAATTTGGATTCAATCATTCATAATC
TTTCAAATAATTTATCAGAGGGTACTGAATACTTTAAGATGTTGGTAAACGTTTTCGCTA
CAGAGTTTCGAAATATCGCTAATCAACATTTAAAGAATTTCTATATCATCGTACNTGCNT
TGGCNTCAAATTACATCGATCATATGATCAATAGTAAAGATAAACTCTTTAAGAAATCAA
AAGCNATCGGTGCTGAAGCTTTATTATTCACTGATGATGGCTTTGCAATTGGTTTGGCTT
ATATCTTAAAATTATTAGATCAAAATAAAGATTTCGATTNTCTTCATTGGTTCGATCGTA
NTCAANTGAAATGTGAAACTGATCAAAAGAGAATGTTGGCTGAAGCNAATGCNAAAGGTA
TCAAAGAGGATCAAAATCATTTAGCNATTAAAAAGATTCAAAATTTCCNACNAGAATATG
AATTATTTAAATATTCTTTC

*ktnnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIE
EALDETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRVLKE
TAESKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFASI
YQSQKNIHTQFFK---

---QLGVSETGLPFLDXFRLLITHIGNAMGYIRLVRSGGLHYCSNAIKFVPDLKKIPKFQ
DLTSKDAFSLETIQASTNLDSIIHNLSNNLSEGTEYFKMLVNVFATEFRNIANQHLKNFY
IIVXALASNYIDHMINSKDKLFKKSKAIGAEALLFTDDGFAIGLAYILKLLDQNKDFDXL
HWFDRXQXKCETDQKRMLAEANAKGIKEDQNHLAIKKIQNFXXEYELFKYSF

Frame A:
nkkrttqfrkwphpirlimvkmknihlmhlkmvhlnwlesnn*lnlinlqlimhnkfgr*
kkh*mkh*mkygi*i*iqfqfiinlmnn*i*lnwlkliikfsik*y*yslqfviklef*k
kqqkvnfihh*qylerlqvnhqkvmyklklvnyyhl*liyqhl*idvih*leillvnlhr
sinhkriyihnslr---

---QLGVSETGLPFLDXFRLLITHIGNAMGYIRLVRSGGLHYCSNAIKFVPDLKKIPKFQ
DLTSKDAFSLETIQASTNLDSIIHNLSNNLSEGTEYFKMLVNVFATEFRNIANQHLKNFY
IIVXALASNYIDHMINSKDKLFKKSKAIGAEALLFTDDGFAIGLAYILKLLDQNKDFDXL
HWFDRXQXKCETDQKRMLAEANAKGIKEDQNHLAIKKIQNFXXEYELFKYSF

Frame B:
ikneqpnlenghilfg**wsk*riyi*ci*rwfi*igwratin*t*sics*lcttnledr
rsir*nik*smgykyrssfnl**ts*tirfn*ig*n*s*nfq*snisilfnl*sn*sfkr
nsrk*ilftinsiwrdyr*iirr*ctn*sw*iitiyd*fisick*mlfin*kyy*sicid
lsitkeytytil*e---

---nwvsxrlvylf*ixldf*slilvmlwvildwsdpvvytivvmlsnsyqt*krfqnsk
i*lqkmpshlkxfklvpiwiqsfiifqiiyqrvlntlrcw*tfslqsfeislini*risi
ssyxxwxqitsii*sivkinslrnqkxsvlklyyslmmalqlvwlis*ny*ikikisixf
igsivxx*nvklikrecwlkxmxkvskrikii*xlkrfkisxxnmnylnil

Frame C:
*ktnnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIE
EALDETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRVLKE
TAESKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFASI
YQSQKNIHTQFFK---

---igclxdwftffrsf*tfnhsyw*cygly*igqirwftll**cyqirtrlekdskipr
fnfkrcllt*nxss*yqfgfnhs*sfk*firgy*il*dvgkrfryrvskyr*stfkefly
hrtcxgxklhrsydq**r*tl*eiksxrc*sfiih**wlcnwfglylkiirsk*rfrxss
lvrsxsxem*n*skenvg*sxcxryqrgsksfsx*kdskfptri*ii*iff

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF123 (VHF123Q) /CSM/VH/VHF1-A/VHF123Q.Seq.d/                       2389   0.0
VHF863 (VHF863Q) /CSM/VH/VHF8-C/VHF863Q.Seq.d/                       1261   0.0
VHF259 (VHF259Q) /CSM/VH/VHF2-C/VHF259Q.Seq.d/                       1257   0.0
VHF714 (VHF714Q) /CSM/VH/VHF7-A/VHF714Q.Seq.d/                       1247   0.0
VHC161 (VHC161Q) /CSM/VH/VHC1-C/VHC161Q.Seq.d/                       1243   0.0
VHF260 (VHF260Q) /CSM/VH/VHF2-C/VHF260Q.Seq.d/                       1235   0.0
VHK538 (VHK538Q) /CSM/VH/VHK5-B/VHK538Q.Seq.d/                       1233   0.0
VHB619 (VHB619Q) /CSM/VH/VHB6-A/VHB619Q.Seq.d/                       1233   0.0
VHB189 (VHB189Q) /CSM/VH/VHB1-D/VHB189Q.Seq.d/                       1233   0.0
SHL542 (SHL542Q) /CSM/SH/SHL5-B/SHL542Q.Seq.d/                       1233   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BU746157|BU746157.1 CH3#002_C07T7 Canine heart normalized cDNA Library in pBluescriptCanis familiaris cDNA clone CH3#002_C07 5', mRNA sequence.    38   8e-04  3
CP000263|CP000263.1 Buchnera aphidicola str. Cc (Cinara cedri), complete genome.    36   8e-04  14
AC168241|AC168241.2 Bos taurus clone CH240-197K22, *** SEQUENCING IN PROGRESS ***, 28 unordered pieces.    52   0.043  1
DN287505|DN287505.1 1241755 MARC 7BOV Bos taurus cDNA 3', mRNA sequence.    34   0.14   3
CR103667|CR103667.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP142o16.    50   0.17   1
CR044130|CR044130.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP186g09.    50   0.17   1
CR034434|CR034434.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP47e19.    50   0.17   1
CR023465|CR023465.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP421e10.    50   0.17   1
CR017539|CR017539.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP47e19.    50   0.17   1
BX990136|BX990136.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP414o21.    50   0.17   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54R74) RecName: Full=UPF0681 protein;                               434   e-120
BC040936_1(BC040936|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   220   7e-56
AB028956_1(AB028956|pid:none) Homo sapiens mRNA for KIAA1033 pro...   220   1e-55
BC143373_1(BC143373|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   220   1e-55
BC020314_1(BC020314|pid:none) Mus musculus RIKEN cDNA A230046K03...   218   4e-55
(Q3UMB9) RecName: Full=UPF0681 protein KIAA1033;                      218   4e-55
AK161247_1(AK161247|pid:none) Mus musculus adult male testis cDN...   218   4e-55
AK145000_1(AK145000|pid:none) Mus musculus mammary gland RCB-052...   218   4e-55
BC110850_1(BC110850|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   163   1e-38
AX882617_1(AX882617|pid:none) Sequence 17522 from Patent EP10746...   158   3e-37

 psg: 0.79  gvh: 0.25  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VHF123 is cyt

>VHF123F.Seq
AATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTC
AAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGC
AACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAG
AAGAAGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAA
TTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAA
TTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAGTTTTAAAAG
AAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAAT
CATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTAT
CAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGA
TCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAANNNNNNNNNN

>VHF123Z.Seq
NNNNNNNNNNCAATTGGGTGTCTCNGAGACTGGTTTACCTTTTTTAGATCNTTTTAGACT
TTTAATCACTCATATTGGTAATGCTATGGGTTATATTAGATTGGTCAGATCCGGTGGTTT
ACACTATTGTAGTAATGCTATCAAATTCGTACCAGACTTGAAAAAGATTCCAAAATTCCA
AGATTTAACTTCAAAAGATGCCTTCTCACTTGAAACNATTCAAGCTAGTACCAATTTGGA
TTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAATACTTTAAGATGTT
GGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACATTTAAAGAATTTCTA
TATCATCGTACNTGCNTTGGCNTCAAATTACATCGATCATATGATCAATAGTAAAGATAA
ACTCTTTAAGAAATCAAAAGCNATCGGTGCTGAAGCTTTATTATTCACTGATGATGGCTT
TGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAAGATTTCGATTNTCT
TCATTGGTTCGATCGTANTCAANTGAAATGTGAAACTGATCAAAAGAGAATGTTGGCTGA
AGCNAATGCNAAAGGTATCAAAGAGGATCAAAATCATTTAGCNATTAAAAAGATTCAAAA
TTTCCNACNAGAATATGAATTATTTAAATATTCTTTC

>VHF123P.Seq
AATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTC
AAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGC
AACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAG
AAGAAGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAA
TTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAA
TTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAGTTTTAAAAG
AAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAAT
CATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTAT
CAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGA
TCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAA----------CAATTGG
GTGTCTCNGAGACTGGTTTACCTTTTTTAGATCNTTTTAGACTTTTAATCACTCATATTG
GTAATGCTATGGGTTATATTAGATTGGTCAGATCCGGTGGTTTACACTATTGTAGTAATG
CTATCAAATTCGTACCAGACTTGAAAAAGATTCCAAAATTCCAAGATTTAACTTCAAAAG
ATGCCTTCTCACTTGAAACNATTCAAGCTAGTACCAATTTGGATTCAATCATTCATAATC
TTTCAAATAATTTATCAGAGGGTACTGAATACTTTAAGATGTTGGTAAACGTTTTCGCTA
CAGAGTTTCGAAATATCGCTAATCAACATTTAAAGAATTTCTATATCATCGTACNTGCNT
TGGCNTCAAATTACATCGATCATATGATCAATAGTAAAGATAAACTCTTTAAGAAATCAA
AAGCNATCGGTGCTGAAGCTTTATTATTCACTGATGATGGCTTTGCAATTGGTTTGGCTT
ATATCTTAAAATTATTAGATCAAAATAAAGATTTCGATTNTCTTCATTGGTTCGATCGTA
NTCAANTGAAATGTGAAACTGATCAAAAGAGAATGTTGGCTGAAGCNAATGCNAAAGGTA
TCAAAGAGGATCAAAATCATTTAGCNATTAAAAAGATTCAAAATTTCCNACNAGAATATG
AATTATTTAAATATTCTTTC