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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF122
Library VH
(Link to library)
Clone ID VHF122
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHF122P
(Link to Original site)
Representative DNA sequence 
>VHF122 (VHF122Q) /CSM/VH/VHF1-A/VHF122Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTXXXXXXXXXXCCAGAAGGTAACGGTGCCAATATCNTC
GTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGANTCTTTCGTTGGTGCT
TTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTC
AATCTTTATGATGTCACCNTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATC
CCAACTGCTCGTCGTGTAATCATATGCTGATGAATTAACTGCCTCCCCAAATNTTTTAGA
ACCAATTTACTTAGTAGAAATTACTGCCCCAGAAAACGCTANTGGTGGTATTTAATCTGT
ANTCAATCGTCGTAGAGGTATTGTTANTGGTGAAGAACGTCGTATCGGTTCCCCATTANT
ATCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGATGATTTACGTTC
CCACANTGCCGGTCAAGNTTTCCCACAATGTGTNTTCGATCANTGGGNTTCAANTGGTGT
TGTCAACAAAGATAAGAAAGCCNNTGAANTCGCTTTAGCCNCCAGAAAGAGAAAAGGTTT
AGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGC
ACTTTGAGTTTTCAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFR---

---PEGNGANIXVNVTKGVQYLNEIKXSFVGAFQWATKEGVVCDENMRGIRFNLYDVTXH
TDAIHRGGGQIIPTARRVIIC**inclpkxfrtnllsrnycprkrxwwylicxqss*ryc
xw*rtsyrfpixicqssftsirilrfhr*ftfpxcrsxfptmcxrsxgfxwccqqr*esx
*xrfsxqkekrfsprnprs*qiprktlnnlsrtlsfq
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklisls---

---PEGNGANIXVNVTKGVQYLNEIKXSFVGAFQWATKEGVVCDENMRGIRFNLYDVTXH
TDAIHRGGGQIIPTARRVIIC**inclpkxfrtnllsrnycprkrxwwylicxqss*ryc
xw*rtsyrfpixicqssftsirilrfhr*ftfpxcrsxfptmcxrsxgfxwccqqr*esx
*xrfsxqkekrfsprnprs*qiprktlnnlsrtlsfq

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp---

---qkvtvpisssmspkvfni*mklkxlslvlsngppkkvlfvmkt*evsvsifmmspst
lmpstevvvkssqllvv*syadeltaspnxlepiylveitapenaxggi*svxnrrrgiv
xgeerrigsplxsvkahlpvlesfgftddlrshxagqxfpqcvfdxwxsxgvvnkdkkax
exalaxrkrkglapeipdldkfhekl*ti*val*vfk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFR---

---rr*rcqyxrqchqrcsife*n*rxfrwcfpmghqrrccl**kherypfqsl*chxph
*chpqrwwsnhpncsscnhmlmn*lppqxf*nqft**kllpqktlxvvfnlxsivvevll
xvknvvsvphxylskliyqy*npsvspmiyvptxpvkxshnvxsixgxqxvlstkirkpx
xsl*pperekv*pqksqiltnstknskqfkshfefs
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF122 (VHF122Q) /CSM/VH/VHF1-A/VHF122Q.Seq.d/                       2204   0.0
VHF738 (VHF738Q) /CSM/VH/VHF7-B/VHF738Q.Seq.d/                       1142   0.0
VHF658 (VHF658Q) /CSM/VH/VHF6-C/VHF658Q.Seq.d/                       1130   0.0
VHF671 (VHF671Q) /CSM/VH/VHF6-C/VHF671Q.Seq.d/                       1128   0.0
VHF314 (VHF314Q) /CSM/VH/VHF3-A/VHF314Q.Seq.d/                       1118   0.0
VHF772 (VHF772Q) /CSM/VH/VHF7-C/VHF772Q.Seq.d/                       1112   0.0
VHF861 (VHF861Q) /CSM/VH/VHF8-C/VHF861Q.Seq.d/                       1110   0.0
VHF616 (VHF616Q) /CSM/VH/VHF6-A/VHF616Q.Seq.d/                       1110   0.0
VHF369 (VHF369Q) /CSM/VH/VHF3-C/VHF369Q.Seq.d/                       1106   0.0
VHF139 (VHF139Q) /CSM/VH/VHF1-B/VHF139Q.Seq.d/                       1104   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.  1074   0.0    4
AF107286|AF107286.1 Candida albicans elongation factor 2 (EFT2) gene, partial cds.    78   2e-59  8
Y09664|Y09664.1 C.albicans EFT2 gene.                              78   7e-58  8
AF107291|AF107291.1 Candida parapsilosis elongation factor 2 (EFT2) gene, partial cds.    72   8e-54  10
AF107289|AF107289.1 Candida tropicalis elongation factor 2 (EFT2) gene, partial cds.    64   6e-51  9
AF107290|AF107290.1 Candida lusitaniae elongation factor 2 (EFT2) gene, partial cds.    64   2e-37  7
AY144999|AY144999.1 Saccharomyces kluyveri clone Contig1706 EFT2 gene, complete cds.    78   4e-34  5
CX155337|CX155337.1 TfEST072F03 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    90   4e-33  4
AF534908|AF534908.2 Tetrahymena thermophila elongation factor 2 gene, complete cds.    84   4e-33  4
CX154324|CX154324.1 TfEST033C12 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    90   6e-33  4
dna update 2007.10.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   249   2e-64
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   245   2e-63
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   244   3e-63
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   243   8e-63
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   243   8e-63
AY618550_1(AY618550|pid:none) Trypanosoma cruzi elongation facto...   243   1e-62
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   243   1e-62
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   243   1e-62
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   242   2e-62
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   242   2e-62
protein update 2009. 7. 2
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHF122 is cyt
5' end seq. ID VHF122F
5' end seq. 
>VHF122F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTNNNNNNNNNN
Length of 5' end seq. 573
3' end seq. ID VHF122Z
3' end seq. 
>VHF122Z.Seq
NNNNNNNNNNCCAGAAGGTAACGGTGCCAATATCNTCGTCAATGTCACCAAAGGTGTTCA
ATATTTGAATGAAATTAAAGANTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGG
TGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCNTCCA
CACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTAATCAT
ATGCTGATGAATTAACTGCCTCCCCAAATNTTTTAGAACCAATTTACTTAGTAGAAATTA
CTGCCCCAGAAAACGCTANTGGTGGTATTTAATCTGTANTCAATCGTCGTAGAGGTATTG
TTANTGGTGAAGAACGTCGTATCGGTTCCCCATTANTATCTGTCAAAGCTCATTTACCAG
TATTAGAATCCTTCGGTTTCACCGATGATTTACGTTCCCACANTGCCGGTCAAGNTTTCC
CACAATGTGTNTTCGATCANTGGGNTTCAANTGGTGTTGTCAACAAAGATAAGAAAGCCN
NTGAANTCGCTTTAGCCNCCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTG
ACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCAAA
Length of 3' end seq. 653
Connected seq. ID VHF122P
Connected seq. 
>VHF122P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGT----------CCAGAAGGTAACGGTGCCAATATCNTC
GTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGANTCTTTCGTTGGTGCT
TTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTC
AATCTTTATGATGTCACCNTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATC
CCAACTGCTCGTCGTGTAATCATATGCTGATGAATTAACTGCCTCCCCAAATNTTTTAGA
ACCAATTTACTTAGTAGAAATTACTGCCCCAGAAAACGCTANTGGTGGTATTTAATCTGT
ANTCAATCGTCGTAGAGGTATTGTTANTGGTGAAGAACGTCGTATCGGTTCCCCATTANT
ATCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGATGATTTACGTTC
CCACANTGCCGGTCAAGNTTTCCCACAATGTGTNTTCGATCANTGGGNTTCAANTGGTGT
TGTCAACAAAGATAAGAAAGCCNNTGAANTCGCTTTAGCCNCCAGAAAGAGAAAAGGTTT
AGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGC
ACTTTGAGTTTTCAAA
Length of connected seq. 1206
Full length Seq ID -
Full length Seq. -
Length of full length seq. -