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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF121
Library VH
(Link to library)
Clone ID VHF121
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16349-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VHF121 (VHF121Q) /CSM/VH/VHF1-A/VHF121Q.Seq.d/
XXXXXXXXXXCACNTGTGATGTTTTGCCAGTTTACTCCTCCCTTTGTAGGGCTATGCNTG
ATATGTCACAATGTGCCAATTGGACCAAGATGTGTTCATCNTCATGGTCAACTCTACAAC
TCTCAAATCACATCAGACTATTGTGTCGCATCTGTTGCTGACGCCGTTCCAATCATGAGA
ATGTACTTTCACACTGGTATCTTGGACTATATCNTCTTTAAATCTTGGGTACCAAGAACT
GACCGTCAATTTGCTGGTTCATGGTTTGCCATCTTTTTCTTTGCTATCTTTTTTGAACTC
GAAAAAACCNTTAGAAGTATTTTAGAAA
sequence update 2002. 9.10
Translated Amino Acid sequence 
---XVMFCQFTPPFVGLCXICHNVPIGPRCVHXHGQLYNSQITSDYCVASVADAVPIMRM
YFHTGILDYIXFKSWVPRTDRQFAGSWFAIFFFAIFFELEKTXRSILE
Translated Amino Acid sequence (All Frames) 
Frame A:
---tcdvlpvysslcramxdmsqcanwtkmcssswstlqlsnhirllcricc*rrsnhen
vlshwylglyxl*ilgtkn*psicwfmvchlflcylf*trknx*kyfr


Frame B:
---XVMFCQFTPPFVGLCXICHNVPIGPRCVHXHGQLYNSQITSDYCVASVADAVPIMRM
YFHTGILDYIXFKSWVPRTDRQFAGSWFAIFFFAIFFELEKTXRSILE


Frame C:
---x*cfaslllpl*gya*yvtmcqldqdvfixmvnsttlkshqtivshllltpfqs*ec
tftlvswtisslnlgyqeltvnllvhglpsfsllsflnskkplevf*k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF121 (VHF121Q) /CSM/VH/VHF1-A/VHF121Q.Seq.d/                        601   e-171
VHQ111 (VHQ111Q) /CSM/VH/VHQ1-A/VHQ111Q.Seq.d/                        561   e-159
VHM772 (VHM772Q) /CSM/VH/VHM7-C/VHM772Q.Seq.d/                        561   e-159
VHJ528 (VHJ528Q) /CSM/VH/VHJ5-B/VHJ528Q.Seq.d/                        561   e-159
VFO827 (VFO827Q) /CSM/VF/VFO8-B/VFO827Q.Seq.d/                        561   e-159
VFO559 (VFO559Q) /CSM/VF/VFO5-C/VFO559Q.Seq.d/                        561   e-159
VFO333 (VFO333Q) /CSM/VF/VFO3-B/VFO333Q.Seq.d/                        561   e-159
VFN264 (VFN264Q) /CSM/VF/VFN2-C/VFN264Q.Seq.d/                        561   e-159
VFN143 (VFN143Q) /CSM/VF/VFN1-B/VFN143Q.Seq.d/                        561   e-159
VFM192 (VFM192Q) /CSM/VF/VFM1-D/VFM192Q.Seq.d/                        561   e-159
own update 2002.12.26
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AJ315489|AJ315489.1 Dictyostelium discoideum mRNA for p80 protein.   424   e-154  2
ES567380|ES567380.1 TPAF-aab89f08.g1 T.spiralis_EST Trichinella spiralis cDNA 5', mRNA sequence.    46   0.64   1
CA842637|CA842637.1 iq45c01.y1 HR85 islet Homo sapiens cDNA clone IMAGE: 5' similar to TR:Q9VWD5 Q9VWD5 CG14232 PROTEIN. ;, mRNA sequence.    46   0.64   1
ER708132|ER708132.1 SB_BBc07-L15.R SB_BBc Sorghum bicolor genomic 3', genomic survey sequence.    46   0.64   1
AC183870|AC183870.3 Felis catus clone RP86-310C16, WORKING DRAFT SEQUENCE, 12 ordered pieces.    42   1.6    2
AC183732|AC183732.2 Felis catus clone RP86-160K24, WORKING DRAFT SEQUENCE, 15 ordered pieces.    42   1.6    2
AG460073|AG460073.1 Mus musculus molossinus DNA, clone:MSMg01-347N04.TJ, genomic survey sequence.    44   2.5    1
BH157336|BH157336.1 ENTSV71TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, genomic survey sequence.    32   6.3    2
AZ544824|AZ544824.1 ENTFO60TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, genomic survey sequence.    32   6.3    2
AZ669973|AZ669973.1 ENTKI43TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, genomic survey sequence.    32   6.3    2
dna update 2007. 9. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q7YXD4) RecName: Full=Protein P80; AltName: Full=Endosomal memb...   127   9e-29
protein update 2009. 3. 1
PSORT 

 psg: 0.93  gvh: 0.51  alm: 0.31  top: 0.40  tms: 0.07  mit: 0.29  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 24.0 %: cytoplasmic
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: extracellular, including cell wall

>> prediction for VHF121 is end
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID VHF121Z
3' end seq. 
>VHF121Z.Seq
NNNNNNNNNNCACNTGTGATGTTTTGCCAGTTTACTCCTCCCTTTGTAGGGCTATGCNTG
ATATGTCACAATGTGCCAATTGGACCAAGATGTGTTCATCNTCATGGTCAACTCTACAAC
TCTCAAATCACATCAGACTATTGTGTCGCATCTGTTGCTGACGCCGTTCCAATCATGAGA
ATGTACTTTCACACTGGTATCTTGGACTATATCNTCTTTAAATCTTGGGTACCAAGAACT
GACCGTCAATTTGCTGGTTCATGGTTTGCCATCTTTTTCTTTGCTATCTTTTTTGAACTC
GAAAAAACCNTTAGAAGTATTTTAGAAA
Length of 3' end seq. 328
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -