>VHF109 (VHF109Q) /CSM/VH/VHF1-A/VHF109Q.Seq.d/
GAAACTCGAAGAAGAACTCAAACAAGTTCAAGAAGCTCTCGCTGCTGAAACTGCTGCTAA
ATTAGCTCAAGAAGCTGCCAACAAGAAATTACAAGGTGAATACACTGAATTAAACGAAAA
ATTCAACTCTGAAGTCACTGCTCGTTCAAATGTTGAAAAATCAAAGAAGACCCTCGAAAG
TCAATTGGTTGCCGTCAACAACGAATTAGATGAAGAGAAGAAGAATCGTGATGCCCTTGA
AAAGAAGAAGAAAGCTTTAGACGCTATGTTAGAGGAAATGAAAGATCAATTAGAATCCAC
TGGTGGTGAAAAGAAATCACTCTATGATCTCAAAGTTAAACAAGAATCAGATATGGAGGC
TTTACGTAATCAAATCTCTGAACTCCAATCAACTATTGCCAAATTAGAAAAGATTAAATC
CACTTTAGAAGGTGAAGTTGCTCGTTTACAAGGTGAATTAGAAGCTGAACAATTAGCCAA
ATCCAACGTTGAAAAACAAAAGAAGAAGGTTGAATTAGATTTGGAAGATAAATCTGCTCA
ATTAGCTGAAGXXXXXXXXXX

KLEEELKQVQEALAAETAAKLAQEAANKKLQGEYTELNEKFNSEVTARSNVEKSKKTLES
QLVAVNNELDEEKKNRDALEKKKKALDAMLEEMKDQLESTGGEKKSLYDLKVKQESDMEA
LRNQISELQSTIAKLEKIKSTLEGEVARLQGELEAEQLAKSNVEKQKKKVELDLEDKSAQ
LAE---

Frame A:
etrrrtqtssrssrc*ncc*issrscqqeitr*ih*ikrkiql*shcsfkc*kikedprk
sigcrqqrir*reees*cp*keeesfrryvrgnersirihww*keitl*sqs*trirygg
ft*snl*tpinycqirkd*ihfrr*scsftr*irs*tisqiqr*ktkeeg*irfgr*ics
is*---


Frame B:
KLEEELKQVQEALAAETAAKLAQEAANKKLQGEYTELNEKFNSEVTARSNVEKSKKTLES
QLVAVNNELDEEKKNRDALEKKKKALDAMLEEMKDQLESTGGEKKSLYDLKVKQESDMEA
LRNQISELQSTIAKLEKIKSTLEGEVARLQGELEAEQLAKSNVEKQKKKVELDLEDKSAQ
LAE---


Frame C:
nskknsnkfkklsllkllln*lkklptrnykvntln*tknstlksllvqmlknqrrpskv
nwlpsttn*mkrrrivmplkrrrkl*tlc*rk*kin*nplvvkrnhsmisklnknqiwrl
yvikslnsnqllpn*krlnpl*kvkllvykvn*klnn*pnptlknkrrrln*iwkinlln
*l---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF109 (VHF109Q) /CSM/VH/VHF1-A/VHF109Q.Seq.d/                       1092   0.0
SHG269 (SHG269Q) /CSM/SH/SHG2-C/SHG269Q.Seq.d/                        940   0.0
VHO137 (VHO137Q) /CSM/VH/VHO1-B/VHO137Q.Seq.d/                        850   0.0
VHM730 (VHM730Q) /CSM/VH/VHM7-B/VHM730Q.Seq.d/                        428   e-119
SHK426 (SHK426Q) /CSM/SH/SHK4-B/SHK426Q.Seq.d/                        389   e-107
SLC782 (SLC782Q) /CSM/SL/SLC7-D/SLC782Q.Seq.d/                        369   e-101
SLB184 (SLB184Q) /CSM/SL/SLB1-D/SLB184Q.Seq.d/                         38   0.14
AFM186 (AFM186Q) /CSM/AF/AFM1-D/AFM186Q.Seq.d/                         38   0.14
VSJ369 (VSJ369Q) /CSM/VS/VSJ3-C/VSJ369Q.Seq.d/                         36   0.55
VSI254 (VSI254Q) /CSM/VS/VSI2-C/VSI254Q.Seq.d/                         36   0.55

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M14628|M14628.1 D.discoideum myosin heavy chain gene, complete cds.  1090   0.0    1
CD066134|CD066134.1 MA1-0048G-M047-F09-U.G MA1-0048 Schistosoma mansoni cDNA clone MA1-0048G-M047-F09.G, mRNA sequence.    44   2e-04  3
CD134704|CD134704.1 MG1-0034T-L311-G10-U.B MG1-0034 Schistosoma mansoni cDNA clone MG1-0034T-L311-G10.B, mRNA sequence.    44   0.004  2
CD191768|CD191768.1 MS1-0073T-L307-G04-U.B MS1-0073 Schistosoma mansoni cDNA clone MS1-0073T-L307-G04.B, mRNA sequence.    44   0.004  2
CD067583|CD067583.1 MA1-0050T-R032-C11-U.B MA1-0050 Schistosoma mansoni cDNA clone MA1-0050T-R032-C11.B, mRNA sequence.    44   0.004  2
CD144867|CD144867.1 MG1-0101U-V382-D01-U.B MG1-0101 Schistosoma mansoni cDNA clone MG1-0101U-V382-D01.B, mRNA sequence.    44   0.005  2
CD134775|CD134775.1 MG1-0034T-L320-F02-U.B MG1-0034 Schistosoma mansoni cDNA clone MG1-0034T-L320-F02.B, mRNA sequence.    44   0.005  2
CD064140|CD064140.1 MA1-0037P-V036-H01-U.B MA1-0037 Schistosoma mansoni cDNA clone MA1-0037P-V036-H01.B, mRNA sequence.    44   0.006  2
CD134649|CD134649.1 MG1-0034T-L311-B12-U.B MG1-0034 Schistosoma mansoni cDNA clone MG1-0034T-L311-B12.B, mRNA sequence.    44   0.006  2
CD184947|CD184947.1 MS1-0049G-V346-D09-U.B MS1-0049 Schistosoma mansoni cDNA clone MS1-0049G-V346-D09.B, mRNA sequence.    44   0.006  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P08799) RecName: Full=Myosin-2 heavy chain; AltName: Full=Myosi...   258   8e-68
A26655(A26655;A24728;S00250) myosin heavy chain [similarity] - s...   258   8e-68
AF335500_1(AF335500|pid:none) Physarum polycephalum major plasmo...    96   8e-19
BC026142_1(BC026142|pid:none) Mus musculus myosin, heavy polypep...    73   6e-12
D85924_1(D85924|pid:none) Mus musculus mRNA for myosin, complete...    73   6e-12
(O08638) RecName: Full=Myosin-11; AltName: Full=Myosin heavy cha...    73   6e-12
AB020673_1(AB020673|pid:none) Homo sapiens mRNA for KIAA0866 pro...    69   8e-11
D10667_1(D10667|pid:none) Homo sapiens mRNA for smooth muscle my...    69   8e-11
X69292_1(X69292|pid:none) H.sapiens mRNA for smooth muscle myosin.     69   8e-11
AY520816_1(AY520816|pid:none) Homo sapiens smooth muscle myosin ...    69   8e-11

 psg: 0.75  gvh: 0.35  alm: 0.57  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for VHF109 is nuc

>VHF109F.Seq
GAAACTCGAAGAAGAACTCAAACAAGTTCAAGAAGCTCTCGCTGCTGAAACTGCTGCTAA
ATTAGCTCAAGAAGCTGCCAACAAGAAATTACAAGGTGAATACACTGAATTAAACGAAAA
ATTCAACTCTGAAGTCACTGCTCGTTCAAATGTTGAAAAATCAAAGAAGACCCTCGAAAG
TCAATTGGTTGCCGTCAACAACGAATTAGATGAAGAGAAGAAGAATCGTGATGCCCTTGA
AAAGAAGAAGAAAGCTTTAGACGCTATGTTAGAGGAAATGAAAGATCAATTAGAATCCAC
TGGTGGTGAAAAGAAATCACTCTATGATCTCAAAGTTAAACAAGAATCAGATATGGAGGC
TTTACGTAATCAAATCTCTGAACTCCAATCAACTATTGCCAAATTAGAAAAGATTAAATC
CACTTTAGAAGGTGAAGTTGCTCGTTTACAAGGTGAATTAGAAGCTGAACAATTAGCCAA
ATCCAACGTTGAAAAACAAAAGAAGAAGGTTGAATTAGATTTGGAAGATAAATCTGCTCA
ATTAGCTGAAGNNNNNNNNNN