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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE891
Library VH
(Link to library)
Clone ID VHE891
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHE891P
(Link to Original site)
Representative DNA sequence 
>VHE891 (VHE891Q) /CSM/VH/VHE8-D/VHE891Q.Seq.d/
AATATCATTATTGTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCAT
CAATTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTG
TTGAAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTG
ATACAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATA
CAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTG
ATATGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAG
TTGAATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCT
TAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTA
CCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTT
CAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTT
TAGXXXXXXXXXXAGGATGAATATGAAGATAAGATGAAAGCTTTAGAAGCAGTTGTCNAT
CCNATCATGAGTAAANTCTATCAAGAAGGAGGTATGCCNCAAGGTGGTGGTATGCCAGGT
GGTATGTCAAATGATTCACCAAAGAGTTCAAACAGACAAAGTAGACGAATTAGATTGACG
GAATTTTTCAGAGGAAAACATCTCTATCNAATAGATTTCTTAAAAAATAATAAGTTAGTA
NAANCAANAAAGACTTCTCAT
sequence update 2002.10.25
Translated Amino Acid sequence 
yhyc*nksky*iiky*KMSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTD
TERLIGDAAKNQVAMNPTNTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQV
EFKGETKVFSPEEISSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTIS
KLNVQRIINEPTAAAIAYGL---

---DEYEDKMKALEAVVXPIMSKXYQEGGMPQGGGMPGGMSNDSPKSSNRQSRRIRLTEF
FRGKHLYXIDFLKNNKLVXXXKTSH
Translated Amino Acid sequence (All Frames) 
Frame A:
niiivkirvnik*lnikkchqlvli*vqhihvlvfgkmivlkllqmikvieplhlmlhll
iqnv*lvmlqriklq*iqpiqylmlnv*lvvnlvtkkfkvi*nighsklfqrmvishifk
lnskvklkfshqkkslqwys*k*kkplklilvkpliml*lpfqlismivnvkqpkmlvqf
qn*mfnvllmnqllllllmv*---

---rmnmkir*kl*kqlsixs*vxsikkevcxkvvvcqvvcqmihqrvqtdkvdeld*rn
fseenisix*is*kiis*xxqxrll

Frame B:
islllk*e*ilnn*ilknvinwy*frynifmcwclak*sc*nyck*sr**nhsilcciy*
yrtfnw*cckesscnesnqysi*c*tfnws*i**qrssk*yetlaiqsyskgw**atyss
*iqr*n*sfltrrnlfngtlknernr*slsw*nh**ccnyrsslfq**stssnqrcwynf
kikcstyy**tncccyclwf---

---g*i*r*desfrsscxsxhe*xlsrrryaxrwwyarwyvk*ftkefkqtk*tn*idgi
fqrktslsnrflkk**vsxxxkdfs

Frame C:
yhyc*nksky*iiky*KMSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTD
TERLIGDAAKNQVAMNPTNTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQV
EFKGETKVFSPEEISSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTIS
KLNVQRIINEPTAAAIAYGL---

---DEYEDKMKALEAVVXPIMSKXYQEGGMPQGGGMPGGMSNDSPKSSNRQSRRIRLTEF
FRGKHLYXIDFLKNNKLVXXXKTSH
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ240 (VHQ240Q) /CSM/VH/VHQ2-B/VHQ240Q.Seq.d/                       1086   0.0
VHP786 (VHP786Q) /CSM/VH/VHP7-D/VHP786Q.Seq.d/                       1086   0.0
VHP719 (VHP719Q) /CSM/VH/VHP7-A/VHP719Q.Seq.d/                       1086   0.0
VHP716 (VHP716Q) /CSM/VH/VHP7-A/VHP716Q.Seq.d/                       1086   0.0
VHP437 (VHP437Q) /CSM/VH/VHP4-B/VHP437Q.Seq.d/                       1086   0.0
VHP420 (VHP420Q) /CSM/VH/VHP4-A/VHP420Q.Seq.d/                       1086   0.0
VHP232 (VHP232Q) /CSM/VH/VHP2-B/VHP232Q.Seq.d/                       1086   0.0
VHO755 (VHO755Q) /CSM/VH/VHO7-C/VHO755Q.Seq.d/                       1086   0.0
VHN865 (VHN865Q) /CSM/VH/VHN8-C/VHN865Q.Seq.d/                       1086   0.0
VHN681 (VHN681Q) /CSM/VH/VHN6-D/VHN681Q.Seq.d/                       1086   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).  1061   0.0    3
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.   904   0.0    3
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.   285   e-142  2
AF025951|AF025951.1 Dictyostelium discoideum heat-shock cognate protein 70 (hsc70) mRNA, complete cds.   285   e-142  2
EH431862|EH431862.1 NPE00000311 Neocallimastix patriciarum ZAP II cDNA library Neocallimastix patriciarum cDNA, mRNA sequence.   236   6e-99  5
AF221541|AF221541.1 Cryptosporidium serpentis heat shock protein 70 (HSP70) gene, partial cds.   145   3e-82  7
DY886418|DY886418.1 CeleSEQ2066 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ2066, mRNA sequence.   153   3e-79  7
DY886204|DY886204.1 CeleSEQ1763 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ1763, mRNA sequence.   153   3e-79  7
DY892477|DY892477.1 CeleSEQ10725 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ10725, mRNA sequence.   153   3e-79  7
DY890060|DY890060.1 CeleSEQ7095 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ7095, mRNA sequence.   153   3e-79  7
dna update 2007. 9. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   360   4e-98
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   358   2e-97
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   338   1e-91
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   338   1e-91
(Q54BE0) RecName: Full=Heat shock cognate 70 kDa protein 3;           320   5e-86
U71151_1(U71151|pid:none) Pneumocystis carinii heat shock protei...   315   1e-84
AY495382_1(AY495382|pid:none) Trypanosoma rangeli isolate Tre he...   315   1e-84
U80967_1(U80967|pid:none) Pneumocystis carinii f. sp. carinii he...   315   1e-84
(P11145) RecName: Full=Heat shock 70 kDa protein 4; AltName: Ful...   313   4e-84
FJ168549_2(FJ168549|pid:none) Bodo saltans clone fosmid 16K02, c...   313   6e-84
protein update 2009. 7. 2
PSORT 

 psg: 0.87  gvh: 0.35  alm: 0.46  top: 0.27  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHE891 is cyt
5' end seq. ID VHE891F
5' end seq. 
>VHE891F.Seq
AATATCATTATTGTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCAT
CAATTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTG
TTGAAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTG
ATACAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATA
CAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTG
ATATGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAG
TTGAATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCT
TAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTA
CCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTT
CAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTT
TAGNNNNNNNNNN
Length of 5' end seq. 613
3' end seq. ID VHE891Z
3' end seq. 
>VHE891Z.Seq
NNNNNNNNNNAGGATGAATATGAAGATAAGATGAAAGCTTTAGAAGCAGTTGTCNATCCN
ATCATGAGTAAANTCTATCAAGAAGGAGGTATGCCNCAAGGTGGTGGTATGCCAGGTGGT
ATGTCAAATGATTCACCAAAGAGTTCAAACAGACAAAGTAGACGAATTAGATTGACGGAA
TTTTTCAGAGGAAAACATCTCTATCNAATAGATTTCTTAAAAAATAATAAGTTAGTANAA
NCAANAAAGACTTCTCAT
Length of 3' end seq. 258
Connected seq. ID VHE891P
Connected seq. 
>VHE891P.Seq
AATATCATTATTGTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCAT
CAATTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTG
TTGAAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTG
ATACAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATA
CAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTG
ATATGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAG
TTGAATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCT
TAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTA
CCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTT
CAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTT
TAG----------AGGATGAATATGAAGATAAGATGAAAGCTTTAGAAGCAGTTGTCNAT
CCNATCATGAGTAAANTCTATCAAGAAGGAGGTATGCCNCAAGGTGGTGGTATGCCAGGT
GGTATGTCAAATGATTCACCAAAGAGTTCAAACAGACAAAGTAGACGAATTAGATTGACG
GAATTTTTCAGAGGAAAACATCTCTATCNAATAGATTTCTTAAAAAATAATAAGTTAGTA
NAANCAANAAAGACTTCTCAT
Length of connected seq. 851
Full length Seq ID -
Full length Seq. -
Length of full length seq. -