NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE871
Library VH
(Link to library)
Clone ID VHE871
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11753-1|Contig-U13627-1
Original site URL
Representative seq. ID VHE871P
(Link to Original site)
Representative DNA sequence 
>VHE871 (VHE871Q) /CSM/VH/VHE8-C/VHE871Q.Seq.d/
AGAACTTTCAGATTTAACCATGTTAAAGAATGGTGAATTGTTAAAAGAATTCATTGAATT
CATTTTACCAGACTATTTACAATCACCAGAGGGTATCATTAAAATCGGTCAATACTTATT
ATCAATCAATCAATACAATCATGTTGTATTGGTTGCTGAACGTGGTAGAATCAGATTGGA
AAAATTAAAGAAAGAACAAGTTGAAAAGGAACAATTGCAACAAAAACTTGCATTTTTATC
CAAAGAAATTGAATCCATTGAAAAGGTATCTGGTTACTCCGATGAAAAGAGAGGCGATTT
AGTTCAATTGGAAAAGGAATTCTCTGTTTATAAAATGTATCCAACTTATTATTCAATGAA
CAAAAGAGAATTTAGTAAACATAATCTCGATATTGCAACCCTTAGAATTAAATCGATCTT
GGAGATGAAAGATGCTTCCAAAGGTGTATTGAATATTCAATCACATTTAGACGACACCTT
AAAGACTTTATTAGATCCAAATCATATCTACCAATTGGCTGTCCTTTTACAAAAAACTGA
AATTGATACTGCTTTAGTTXXXXXXXXXXCTCAATATTTAGAAGATCCNAAAACTTTTAC
CCNAATTTTAACTCTTCTTAGAGTTAGTAATGATTTTGAAAATGTTACAGAAGTTGGTAA
ATTAGCATTTGCAAAATTAAAATTATTTAGAGAACATCAAAATAAAGTTAAAGAATTGAA
TGAAAATAAGAAATTATTAGTAGAAGAACGTGACC
sequence update 2002.10.25
Translated Amino Acid sequence 
ELSDLTMLKNGELLKEFIEFILPDYLQSPEGIIKIGQYLLSINQYNHVVLVAERGRIRLE
KLKKEQVEKEQLQQKLAFLSKEIESIEKVSGYSDEKRGDLVQLEKEFSVYKMYPTYYSMN
KREFSKHNLDIATLRIKSILEMKDASKGVLNIQSHLDDTLKTLLDPNHIYQLAVLLQKTE
IDTALV---

---QYLEDPKTFTXILTLLRVSNDFENVTEVGKLAFAKLKLFREHQNKVKELNENKKLLV
EERD
Translated Amino Acid sequence (All Frames) 
Frame A:
rtfrfnhvkew*ivkrih*ihftrlftitrgyh*nrsiliinqsiqsccigc*tw*nqig
kikerts*kgtiatktcifiqrn*ih*kgiwllr*kerrfssigkgilcl*nvsnllfne
qkri**t*srycnp*n*idlgdercfqrcieysitfrrhlkdfirsksylpigcpftkn*
n*ycfs---

---lni*kixkllpxf*lflelvmilkmlqklvn*hlqn*nylenikiklkn*mkirny*
*knvt

Frame B:
ELSDLTMLKNGELLKEFIEFILPDYLQSPEGIIKIGQYLLSINQYNHVVLVAERGRIRLE
KLKKEQVEKEQLQQKLAFLSKEIESIEKVSGYSDEKRGDLVQLEKEFSVYKMYPTYYSMN
KREFSKHNLDIATLRIKSILEMKDASKGVLNIQSHLDDTLKTLLDPNHIYQLAVLLQKTE
IDTALV---

---sifrrsxnfypnfnss*s***f*kcyrsw*isickikii*rtsk*s*rie*k*eiis
rrt*

Frame C:
nfqi*pc*rmvnc*knslnsfyqtiynhqrvslksvntyyqsintimlywllnvvesdwk
n*rknklkrnncnknlhfypkklnplkrylvtpmkreai*fnwkrnslfikciqliiq*t
kenlvniisilqplelnrswr*kmlpkvy*ifnhi*ttp*rly*iqiistnwlsfykklk
lill*---

---QYLEDPKTFTXILTLLRVSNDFENVTEVGKLAFAKLKLFREHQNKVKELNENKKLLV
EERD
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE871 (VHE871Q) /CSM/VH/VHE8-C/VHE871Q.Seq.d/                       1425   0.0
VHC140 (VHC140Q) /CSM/VH/VHC1-B/VHC140Q.Seq.d/                        654   0.0
CHG787 (CHG787Q) /CSM/CH/CHG7-D/CHG787Q.Seq.d/                        533   e-150
VHK884 (VHK884Q) /CSM/VH/VHK8-D/VHK884Q.Seq.d/                        504   e-141
VHL193 (VHL193Q) /CSM/VH/VHL1-D/VHL193Q.Seq.d/                        367   e-100
VHF517 (VHF517Q) /CSM/VH/VHF5-A/VHF517Q.Seq.d/                        359   4e-98
VHJ311 (VHJ311Q) /CSM/VH/VHJ3-A/VHJ311Q.Seq.d/                        357   2e-97
VHG869 (VHG869Q) /CSM/VH/VHG8-C/VHG869Q.Seq.d/                        355   6e-97
VHD626 (VHD626Q) /CSM/VH/VHD6-B/VHD626Q.Seq.d/                        355   6e-97
VHK224 (VHK224Q) /CSM/VH/VHK2-A/VHK224Q.Seq.d/                         42   0.012
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AZ713170|AZ713170.1 RPCI-24-151L19.TJ RPCI-24 Mus musculus genomic cloneRPCI-24-151L19, genomic survey sequence.    44   0.001  2
ED811196|ED811196.1 ML__Ba0060L15f ML__Ba Mimulus lewisii genomic, genomic survey sequence.    50   0.099  1
CP000724|CP000724.1 Alkaliphilus metalliredigens QYMF, complete genome.    48   0.39   1
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    40   0.46   10
CT963071|CT963071.2 Pig DNA sequence *** SEQUENCING IN PROGRESS *** from clone  CH242-455A7.    44   0.64   3
AC159486|AC159486.2 Bos taurus clone CH240-68O22, *** SEQUENCING IN PROGRESS ***, 51 unordered pieces.    34   0.79   2
CR352210|CR352210.11 Zebrafish DNA sequence from clone DKEY-166N8 in linkage group 23.    44   1.00   5
DV085433|DV085433.1 327-384-16_K03_T7 Nematostella vectensis normalized cDNA library 327 Nematostella vectensis cDNA clone 327-384-16_K03_T7, mRNA sequence.    46   1.5    1
AC160694|AC160694.2 Bos taurus clone CH240-91O17, *** SEQUENCING IN PROGRESS ***, 58 unordered pieces.    46   1.5    1
AM678366|AM678366.1 Entamoeba terrapinae GSS, clone terra163a01.p1k.    46   1.5    1
dna update 2007. 9. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM450850_1(AM450850|pid:none) Vitis vinifera contig VV78X157592....    33   7.5
EU864034_12(EU864034|pid:none) Azospirillum brasilense histone a...    33   9.7
AF167159_1(AF167159|pid:none) Sinorhizobium meliloti Lon proteas...    33   9.7
(P77810) RecName: Full=ATP-dependent protease La;          EC=3....    33   9.7
protein update 2009. 3. 1
PSORT 

 psg: 0.75  gvh: 0.31  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.12  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VHE871 is nuc
5' end seq. ID VHE871F
5' end seq. 
>VHE871F.Seq
AGAACTTTCAGATTTAACCATGTTAAAGAATGGTGAATTGTTAAAAGAATTCATTGAATT
CATTTTACCAGACTATTTACAATCACCAGAGGGTATCATTAAAATCGGTCAATACTTATT
ATCAATCAATCAATACAATCATGTTGTATTGGTTGCTGAACGTGGTAGAATCAGATTGGA
AAAATTAAAGAAAGAACAAGTTGAAAAGGAACAATTGCAACAAAAACTTGCATTTTTATC
CAAAGAAATTGAATCCATTGAAAAGGTATCTGGTTACTCCGATGAAAAGAGAGGCGATTT
AGTTCAATTGGAAAAGGAATTCTCTGTTTATAAAATGTATCCAACTTATTATTCAATGAA
CAAAAGAGAATTTAGTAAACATAATCTCGATATTGCAACCCTTAGAATTAAATCGATCTT
GGAGATGAAAGATGCTTCCAAAGGTGTATTGAATATTCAATCACATTTAGACGACACCTT
AAAGACTTTATTAGATCCAAATCATATCTACCAATTGGCTGTCCTTTTACAAAAAACTGA
AATTGATACTGCTTTAGTTNNNNNNNNNN
Length of 5' end seq. 569
3' end seq. ID VHE871Z
3' end seq. 
>VHE871Z.Seq
NNNNNNNNNNCTCAATATTTAGAAGATCCNAAAACTTTTACCCNAATTTTAACTCTTCTT
AGAGTTAGTAATGATTTTGAAAATGTTACAGAAGTTGGTAAATTAGCATTTGCAAAATTA
AAATTATTTAGAGAACATCAAAATAAAGTTAAAGAATTGAATGAAAATAAGAAATTATTA
GTAGAAGAACGTGACC
Length of 3' end seq. 196
Connected seq. ID VHE871P
Connected seq. 
>VHE871P.Seq
AGAACTTTCAGATTTAACCATGTTAAAGAATGGTGAATTGTTAAAAGAATTCATTGAATT
CATTTTACCAGACTATTTACAATCACCAGAGGGTATCATTAAAATCGGTCAATACTTATT
ATCAATCAATCAATACAATCATGTTGTATTGGTTGCTGAACGTGGTAGAATCAGATTGGA
AAAATTAAAGAAAGAACAAGTTGAAAAGGAACAATTGCAACAAAAACTTGCATTTTTATC
CAAAGAAATTGAATCCATTGAAAAGGTATCTGGTTACTCCGATGAAAAGAGAGGCGATTT
AGTTCAATTGGAAAAGGAATTCTCTGTTTATAAAATGTATCCAACTTATTATTCAATGAA
CAAAAGAGAATTTAGTAAACATAATCTCGATATTGCAACCCTTAGAATTAAATCGATCTT
GGAGATGAAAGATGCTTCCAAAGGTGTATTGAATATTCAATCACATTTAGACGACACCTT
AAAGACTTTATTAGATCCAAATCATATCTACCAATTGGCTGTCCTTTTACAAAAAACTGA
AATTGATACTGCTTTAGTT----------CTCAATATTTAGAAGATCCNAAAACTTTTAC
CCNAATTTTAACTCTTCTTAGAGTTAGTAATGATTTTGAAAATGTTACAGAAGTTGGTAA
ATTAGCATTTGCAAAATTAAAATTATTTAGAGAACATCAAAATAAAGTTAAAGAATTGAA
TGAAAATAAGAAATTATTAGTAGAAGAACGTGACC
Length of connected seq. 745
Full length Seq ID -
Full length Seq. -
Length of full length seq. -