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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE870
Library VH
(Link to library)
Clone ID VHE870
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16436-1
Original site URL
Representative seq. ID VHE870P
(Link to Original site)
Representative DNA sequence 
>VHE870 (VHE870Q) /CSM/VH/VHE8-C/VHE870Q.Seq.d/
ATAAATAAATTTATAATAATAGAAATTAAAATTTTTTATTTTCTATTTTCTTTTTATAAT
AAAAAAAATGAATGAAAATGATAATAATTTAGAGAATGAAACAGGATTTTCAGGTAGATT
AATTGTTGTTTCAAATAGATTACCAGTATCAATTAAAAAGGAATCAAATGGTAAATGGAG
TTGTAAGATGAGTAGTGGAGGTTTGGTAGCAGCATTATCAGGGTTAAAATCAAATTTTAT
ATGGGTAGGATGGATTGGAGCAGAGATTGAAGAAGATGATCGAAAAGAGATTAAAGAATT
ACTTTGGAAAGATTATAGTTGTATACCAGTATTCCTCTCGGAAAAGGTTGCCAATGAACA
TTACAATGGATTCTCAAATGGAGTACTTTGGCCATTATTTCATTATTTACCAGGAGATCT
CGATTATGATGATAGAATTTGGAATTCCTATGTGGAAGCAAATGAACAATTCTCAAGTGT
AGTTGCAGAGATATTGAAGCCAAATGATTTAGTATGGGTACACGATTATCATATGATGTT
ATTACCAGAGATATTAAAACAAAAGAAACCAGATGCAAGAATTGGGTTCTTTCTACACAT
TCCATTTCCATCATCAGAGATCTTTCGTGTAXXXXXXXXXXAGTGGTCGTGATAAGAAAA
NTCTTAGTGAATGGTTTTTGGGTATTCAGATTGGTTTATCAGCAGAATATGGTTGTTTCT
TTAAATTACCAGAATCAACTGAATGGGAACNACNAGTTCCATCAATGGATTTAAGTTGGA
AAGAAACTATTCGTCCACTCTTTAAATATTTCACTCTTCGTACTCCAGGTTCTTTCTTTG
AAGAAAAAGAAATGTTATTCACATGGCATTATAGAAATGCTGATCCAATATTTGGTTCAA
TTCAAGCACGTGAATTACATTTACATTTAGATAATTTACNTTTAGATGTTATAGTTGGTG
ATAAAACATTGGGTGTAAGATCATATAATATAAATCCATTATCNTCAATGAAAAAAGTTA
TAACAGACACAATTCCAAAAGGATTAGATTTAATTTTATTAATTGGTGATACTCATATTC
ATCCAAGTGAATTGCCAACATTTGATGGTAAAATCTTTAATATTTCAGTTGGTAAAAAAT
CTGTTAAAG
sequence update 2002.10.25
Translated Amino Acid sequence 
*inl***KLKFFIFYFLFIIKKMNENDNNLENETGFSGRLIVVSNRLPVSIKKESNGKWS
CKMSSGGLVAALSGLKSNFIWVGWIGAEIEEDDRKEIKELLWKDYSCIPVFLSEKVANEH
YNGFSNGVLWPLFHYLPGDLDYDDRIWNSYVEANEQFSSVVAEILKPNDLVWVHDYHMML
LPEILKQKKPDARIGFFLHIPFPSSEIFRV---

---SGRDKKXLSEWFLGIQIGLSAEYGCFFKLPESTEWEXXVPSMDLSWKETIRPLFKYF
TLRTPGSFFEEKEMLFTWHYRNADPIFGSIQARELHLHLDNLXLDVIVGDKTLGVRSYNI
NPLSSMKKVITDTIPKGLDLILLIGDTHIHPSELPTFDGKIFNISVGKKSVK
Translated Amino Acid sequence (All Frames) 
Frame A:
inkfiiieikifyflfsfynkkne*k***fre*nrifr*inccfk*itsin*kgikw*me
l*de*wrfgssiirvkikfymgrmdwsrd*rr*skrd*ritlerl*lytsiplgkgcq*t
lqwilkwstlaiislftrrsrl***nleflcgsk*tilkcscrdieak*fsmgtrlsydv
itrdiktketrcknwvlsthsisiirdlsc---

---SGRDKKXLSEWFLGIQIGLSAEYGCFFKLPESTEWEXXVPSMDLSWKETIRPLFKYF
TLRTPGSFFEEKEMLFTWHYRNADPIFGSIQARELHLHLDNLXLDVIVGDKTLGVRSYNI
NPLSSMKKVITDTIPKGLDLILLIGDTHIHPSELPTFDGKIFNISVGKKSVK

Frame B:
*inl***KLKFFIFYFLFIIKKMNENDNNLENETGFSGRLIVVSNRLPVSIKKESNGKWS
CKMSSGGLVAALSGLKSNFIWVGWIGAEIEEDDRKEIKELLWKDYSCIPVFLSEKVANEH
YNGFSNGVLWPLFHYLPGDLDYDDRIWNSYVEANEQFSSVVAEILKPNDLVWVHDYHMML
LPEILKQKKPDARIGFFLHIPFPSSEIFRV---

---vvvirkxlvngfwvfrlvyqqnmvvslnyqnqlngnxxfhqwi*vgkklfvhslnis
lfvlqvlslkkkkcyshgiiemliqylvqfkhvnyiyi*iiyx*ml*lvikhwv*dhii*
ihyxq*kkl*qtqfqkd*i*fy*lvilifiqvncqhlmvkslifqlvknllk

Frame C:
k*iynnrn*nflfsiffl**kk*mkmiii*rmkqdfqvd*llfqidyqyqlkrnqmvngv
vr*vvevw*qhyqg*nqilyg*dgleqrlkkmiekrlknyfgkiivvyqyssrkrlpmni
tmdsqmeyfghyfiiyqeisimmiefgipmwkqmnnsqv*lqry*sqmi*ygytiii*cy
yqry*nkrnqmqelgsfytfhfhhqrsfv---

---ws**exs**mvfgysdwfisriwlfl*itrin*mgttssingfklernysstl*ifh
ssysrffl*rkrnvihmal*kc*sniwfnsst*itftfr*ftfrcysw**nigckii*yk
siixneksynrhnskrirfnfinw*ysyssk*iani*w*nl*yfsw*kic*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE870 (VHE870Q) /CSM/VH/VHE8-C/VHE870Q.Seq.d/                       2054   0.0
VHQ339 (VHQ339Q) /CSM/VH/VHQ3-B/VHQ339Q.Seq.d/                       1116   0.0
CHH678 (CHH678Q) /CSM/CH/CHH6-D/CHH678Q.Seq.d/                       1116   0.0
CHE839 (CHE839Q) /CSM/CH/CHE8-B/CHE839Q.Seq.d/                       1116   0.0
AHQ710 (AHQ710Q) /CSM/AH/AHQ7-A/AHQ710Q.Seq.d/                       1116   0.0
AHG817 (AHG817Q) /CSM/AH/AHG8-A/AHG817Q.Seq.d/                       1116   0.0
AHF706 (AHF706Q) /CSM/AH/AHF7-A/AHF706Q.Seq.d/                       1116   0.0
AHC557 (AHC557Q) /CSM/AH/AHC5-C/AHC557Q.Seq.d/                       1116   0.0
AFM191 (AFM191Q) /CSM/AF/AFM1-D/AFM191Q.Seq.d/                       1116   0.0
AHQ653 (AHQ653Q) /CSM/AH/AHQ6-C/AHQ653Q.Seq.d/                       1108   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

EE007661|EE007661.1 ROE00006399 Rhizopus oryzae Company Rhizopus oryzae cDNA, mRNA sequence.    38   3e-06  3
EE002230|EE002230.1 ROE00011371 Rhizopus oryzae Company Rhizopus oryzae cDNA, mRNA sequence.    38   4e-06  3
AE017308|AE017308.1 Mycoplasma mobile 163K complete genome.        34   0.001  16
AC026955|AC026955.10 Homo sapiens chromosome 17, clone RP11-585D22, complete sequence.    48   0.13   4
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    36   0.31   11
AC105361|AC105361.2 Homo sapiens chromosome , clone RP11-170D6, complete sequence.    48   0.34   4
EC743642|EC743642.1 PPE00007628 Agencourt Biosciences Agen-0020 Non-normalized library cloned into Not I - Eco RV sites of pAGEN-1 Physarum polycephalum cDNA, mRNA sequence.    48   0.60   1
DY895001|DY895001.1 CeleSEQ14711 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ14711, mRNA sequence.    48   0.60   1
AC173045|AC173045.3 Bos taurus clone CH240-130J8, *** SEQUENCING IN PROGRESS ***, 66 unordered pieces.    48   0.60   1
AC162253|AC162253.2 Bos taurus clone CH240-109E19, *** SEQUENCING IN PROGRESS ***, 12 unordered pieces.    48   0.60   1
dna update 2007. 9. 2
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54K57) RecName: Full=Alpha,alpha-trehalose-phosphate synthase ...   362   1e-98
CR380956_419(CR380956|pid:none) Candida glabrata strain CBS138 c...   198   3e-49
CP000496_558(CP000496|pid:none) Pichia stipitis CBS 6054 chromos...   194   5e-48
(Q00075) RecName: Full=Alpha,alpha-trehalose-phosphate synthase ...   194   6e-48
AM270183_12(AM270183|pid:none) Aspergillus niger contig An08c028...   194   6e-48
(Q92410) RecName: Full=Alpha,alpha-trehalose-phosphate synthase ...   193   8e-48
FM992695_507(FM992695|pid:none) Candida dubliniensis CD36 chromo...   193   8e-48
T46564(T46564) alpha,alpha-trehalose-phosphate synthase (UDP-for...   192   2e-47
(O74932) RecName: Full=Alpha,alpha-trehalose-phosphate synthase ...   191   4e-47
AM920437_202(AM920437|pid:none) Penicillium chrysogenum Wisconsi...   191   5e-47
protein update 2009. 7. 2
PSORT 

 psg: 1.00  gvh: 0.35  alm: 0.39  top: 0.20  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 24.0 %: cytoplasmic
	 24.0 %: nuclear
	 16.0 %: extracellular, including cell wall
	 16.0 %: vacuolar
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for VHE870 is cyt
5' end seq. ID VHE870F
5' end seq. 
>VHE870F.Seq
ATAAATAAATTTATAATAATAGAAATTAAAATTTTTTATTTTCTATTTTCTTTTTATAAT
AAAAAAAATGAATGAAAATGATAATAATTTAGAGAATGAAACAGGATTTTCAGGTAGATT
AATTGTTGTTTCAAATAGATTACCAGTATCAATTAAAAAGGAATCAAATGGTAAATGGAG
TTGTAAGATGAGTAGTGGAGGTTTGGTAGCAGCATTATCAGGGTTAAAATCAAATTTTAT
ATGGGTAGGATGGATTGGAGCAGAGATTGAAGAAGATGATCGAAAAGAGATTAAAGAATT
ACTTTGGAAAGATTATAGTTGTATACCAGTATTCCTCTCGGAAAAGGTTGCCAATGAACA
TTACAATGGATTCTCAAATGGAGTACTTTGGCCATTATTTCATTATTTACCAGGAGATCT
CGATTATGATGATAGAATTTGGAATTCCTATGTGGAAGCAAATGAACAATTCTCAAGTGT
AGTTGCAGAGATATTGAAGCCAAATGATTTAGTATGGGTACACGATTATCATATGATGTT
ATTACCAGAGATATTAAAACAAAAGAAACCAGATGCAAGAATTGGGTTCTTTCTACACAT
TCCATTTCCATCATCAGAGATCTTTCGTGTANNNNNNNNNN
Length of 5' end seq. 641
3' end seq. ID VHE870Z
3' end seq. 
>VHE870Z.Seq
NNNNNNNNNNAGTGGTCGTGATAAGAAAANTCTTAGTGAATGGTTTTTGGGTATTCAGAT
TGGTTTATCAGCAGAATATGGTTGTTTCTTTAAATTACCAGAATCAACTGAATGGGAACN
ACNAGTTCCATCAATGGATTTAAGTTGGAAAGAAACTATTCGTCCACTCTTTAAATATTT
CACTCTTCGTACTCCAGGTTCTTTCTTTGAAGAAAAAGAAATGTTATTCACATGGCATTA
TAGAAATGCTGATCCAATATTTGGTTCAATTCAAGCACGTGAATTACATTTACATTTAGA
TAATTTACNTTTAGATGTTATAGTTGGTGATAAAACATTGGGTGTAAGATCATATAATAT
AAATCCATTATCNTCAATGAAAAAAGTTATAACAGACACAATTCCAAAAGGATTAGATTT
AATTTTATTAATTGGTGATACTCATATTCATCCAAGTGAATTGCCAACATTTGATGGTAA
AATCTTTAATATTTCAGTTGGTAAAAAATCTGTTAAAG
Length of 3' end seq. 518
Connected seq. ID VHE870P
Connected seq. 
>VHE870P.Seq
ATAAATAAATTTATAATAATAGAAATTAAAATTTTTTATTTTCTATTTTCTTTTTATAAT
AAAAAAAATGAATGAAAATGATAATAATTTAGAGAATGAAACAGGATTTTCAGGTAGATT
AATTGTTGTTTCAAATAGATTACCAGTATCAATTAAAAAGGAATCAAATGGTAAATGGAG
TTGTAAGATGAGTAGTGGAGGTTTGGTAGCAGCATTATCAGGGTTAAAATCAAATTTTAT
ATGGGTAGGATGGATTGGAGCAGAGATTGAAGAAGATGATCGAAAAGAGATTAAAGAATT
ACTTTGGAAAGATTATAGTTGTATACCAGTATTCCTCTCGGAAAAGGTTGCCAATGAACA
TTACAATGGATTCTCAAATGGAGTACTTTGGCCATTATTTCATTATTTACCAGGAGATCT
CGATTATGATGATAGAATTTGGAATTCCTATGTGGAAGCAAATGAACAATTCTCAAGTGT
AGTTGCAGAGATATTGAAGCCAAATGATTTAGTATGGGTACACGATTATCATATGATGTT
ATTACCAGAGATATTAAAACAAAAGAAACCAGATGCAAGAATTGGGTTCTTTCTACACAT
TCCATTTCCATCATCAGAGATCTTTCGTGTA----------AGTGGTCGTGATAAGAAAA
NTCTTAGTGAATGGTTTTTGGGTATTCAGATTGGTTTATCAGCAGAATATGGTTGTTTCT
TTAAATTACCAGAATCAACTGAATGGGAACNACNAGTTCCATCAATGGATTTAAGTTGGA
AAGAAACTATTCGTCCACTCTTTAAATATTTCACTCTTCGTACTCCAGGTTCTTTCTTTG
AAGAAAAAGAAATGTTATTCACATGGCATTATAGAAATGCTGATCCAATATTTGGTTCAA
TTCAAGCACGTGAATTACATTTACATTTAGATAATTTACNTTTAGATGTTATAGTTGGTG
ATAAAACATTGGGTGTAAGATCATATAATATAAATCCATTATCNTCAATGAAAAAAGTTA
TAACAGACACAATTCCAAAAGGATTAGATTTAATTTTATTAATTGGTGATACTCATATTC
ATCCAAGTGAATTGCCAACATTTGATGGTAAAATCTTTAATATTTCAGTTGGTAAAAAAT
CTGTTAAAG
Length of connected seq. 1139
Full length Seq ID -
Full length Seq. -
Length of full length seq. -