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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE860
Library VH
(Link to library)
Clone ID VHE860
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U12398-1|Contig-U13609-1
Original site URL
Representative seq. ID VHE860P
(Link to Original site)
Representative DNA sequence 
>VHE860 (VHE860Q) /CSM/VH/VHE8-C/VHE860Q.Seq.d/
AATGAAATCACAAAAAATAGGATCAATGATTCTATTAATTGGAATATTATTAGCAATTTT
TAATTTTGCTTATTCAGATGATGACATAGAACGTTTTTCAATCAATCCAGAAAAACCAAT
TTCCTTCACATCAGATCAACCAGGTTTTCCAACTTCAGCAGATTTCCCAATTGGTTCAAT
TCTAGCAAATTCTTTTTATAGTTTTGGTGGAGATGTAAATTACTTTCAATTAAATATAAG
TTTAATGGAAGAATTTTCAAAAGATGGAAACACAGGCAGCCAAGCAACATGGAATCAATA
TACTTCAGTTCCAGTTTCTCCAATTTCATCAGCCGTTACTGCAAACCGTGTTTACACAAT
GTCAATTGGTTCCCTCAACAGAGTTAAAAAAGGTGATATTACTTCAATGGAATCCACAGA
CTTTCTCAATGATGAAAAATACAGTTCACTTGTAACTACTCTTAATGGTGGTGTATCATA
TGGTGATGACGTTTTCTTTTTATCCTCAAAGAGTGCTACTGGTGAGGCTGTATTGATTCA
CATTAATGATACTGCTACTGGTACATTTGGTACAAGCAGTTATGATGAAATTTTACTTGA
TGCTGCAATTAATGATCCAXXXXXXXXXXTTTAAACAAAGATTTGGCTGTCTCTACGTTC
ANTACNAACCAAAGACNTGATCATCGNTTCGNTGTTTTCATGTTTATCAAGAGATTCATT
ATGGCCGTTATCANTGGTATCTTATCATTCAAACCAATGACCANNCTATCCATTAACTGG
TACTGATTTAGCCGTTCCAATCGTTCAAGTAGTTGTCATTGATATCGCTATCATTGGNTA
TGCTGTATTATTATTCATCCGTAAACCATATTTCGATCATTATCAATTATGGCTCGAATA
TATCTTAACTGCCATCAATATTGTTACANTTTATATNTCANTCACTCACATCAAATCACC
AAGCGCCGCTGGTGAATTAATTGCATGTCTTATTCAAGCTTTAGCCNTTGTAGCTTGTAT
TGCCGCTTACGTCGTAGCANGGCTCCAAATGAGATCTTCTTTCATTAAAAAAGTTAAGAA
ATATCTCTGCTGTTGTTGTAAATCCTCAAAATCTTCAGGGAAATCGATTTATCTAAGAAA
TAAAGAATTAAAAATTTATCAATAATAANAATAA
sequence update 2002.10.25
Translated Amino Acid sequence 
MKSQKIGSMILLIGILLAIFNFAYSDDDIERFSINPEKPISFTSDQPGFPTSADFPIGSI
LANSFYSFGGDVNYFQLNISLMEEFSKDGNTGSQATWNQYTSVPVSPISSAVTANRVYTM
SIGSLNRVKKGDITSMESTDFLNDEKYSSLVTTLNGGVSYGDDVFFLSSKSATGEAVLIH
INDTATGTFGTSSYDEILLDAAINDP---

---LNKDLAVSTFXTNQRXDHRFXVFMFIKRFIMAVIXGILSFKPMTXLSINWY*fsrsn
rsssch*yryhwxcciiihp*tifrslsimariylnchqycyxlyxxhshqitkrrw*in
cmsyssfsxcslycrlrrsxapneiffh*ks*eisllll*ilkifreidlskk*riknls
iixi
Translated Amino Acid sequence (All Frames) 
Frame A:
neitknrindsinwniisnf*fclfr**hrtffnqsrktnflhirstrfsnfsrfpnwfn
sskffl*fwwrckllsikykfngrifkrwkhrqpsnmesiyfsssfsnfisryckpclhn
vnwfpqqs*kr*yyfngihrlsq**kiqftcnys*wwciiw**rflfilkecyw*gcids
h**ycywyiwykql**nft*ccn**s---

---fkqrfgclyvxyxpkt*ssxrcfhvyqeihygryxwyliiqtndxxih*lvli*pfq
sfk*lslislslxmlyyyssvnhisiiinygsnis*lpsillxfixxsltsnhqaplvn*
lhvlfkl*pl*lvlplts*xgsk*dllslkklrnisavvvnpqnlqgnrfi*eikn*kfi
nnxn

Frame B:
MKSQKIGSMILLIGILLAIFNFAYSDDDIERFSINPEKPISFTSDQPGFPTSADFPIGSI
LANSFYSFGGDVNYFQLNISLMEEFSKDGNTGSQATWNQYTSVPVSPISSAVTANRVYTM
SIGSLNRVKKGDITSMESTDFLNDEKYSSLVTTLNGGVSYGDDVFFLSSKSATGEAVLIH
INDTATGTFGTSSYDEILLDAAINDP---

---LNKDLAVSTFXTNQRXDHRFXVFMFIKRFIMAVIXGILSFKPMTXLSINWY*fsrsn
rsssch*yryhwxcciiihp*tifrslsimariylnchqycyxlyxxhshqitkrrw*in
cmsyssfsxcslycrlrrsxapneiffh*ks*eisllll*ilkifreidlskk*riknls
iixi

Frame C:
*nhkk*dq*fy*leyy*qflilliqmmt*nvfqsiqknqfpshqinqvfqlqqisqlvqf
*qilfivlvem*itfn*i*v*wknfqkmetqaakqhginilqfqflqfhqpllqtvftqc
qlvpstelkkvillqwnpqtfsmmkntvhl*lllmvvyhmvmtfsfypqrvllvrly*ft
lmilllvhlvqavmmkfylmlqlmi---

---*tkiwlslrsxxtkdxiixsxfsclsrdslwplsxvsyhsnq*pxypltgtdlavpi
vqvvvidiaiigyavllfirkpyfdhyqlwleyiltainivtxyxsxthikspsaageli
acliqalaxvaciaayvvaxlqmrssfikkvkkylccccksskssgksiylrnkelkiyq
**x*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE860 (VHE860Q) /CSM/VH/VHE8-C/VHE860Q.Seq.d/                       2179   0.0
VHP302 (VHP302Q) /CSM/VH/VHP3-A/VHP302Q.Seq.d/                       1199   0.0
VHH579 (VHH579Q) /CSM/VH/VHH5-D/VHH579Q.Seq.d/                       1140   0.0
VHQ122 (VHQ122Q) /CSM/VH/VHQ1-A/VHQ122Q.Seq.d/                        900   0.0
VHD711 (VHD711Q) /CSM/VH/VHD7-A/VHD711Q.Seq.d/                        894   0.0
CHQ351 (CHQ351Q) /CSM/CH/CHQ3-C/CHQ351Q.Seq.d/                        839   0.0
VHD395 (VHD395Q) /CSM/VH/VHD3-D/VHD395Q.Seq.d/                        731   0.0
VHD222 (VHD222Q) /CSM/VH/VHD2-A/VHD222Q.Seq.d/                        728   0.0
VHF655 (VHF655Q) /CSM/VH/VHF6-C/VHF655Q.Seq.d/                        716   0.0
VHL774 (VHL774Q) /CSM/VH/VHL7-D/VHL774Q.Seq.d/                        706   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY178767|AY178767.1 Dictyostelium discoideum substrate adhesion molecule precursor (sadA) gene, complete cds.  1219   0.0    1
CX781713|CX781713.1 HESC3_8_D09.g1_A036 NIH_MGC_260 Homo sapiens cDNA clone IMAGE:7476164 5', mRNA sequence.    48   0.61   1
CR762413|CR762413.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone DKEY-197P18.    48   0.61   1
EK255242|EK255242.1 1095462007001 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061005733670 5', genomic survey sequence.    48   0.61   1
AQ712653|AQ712653.1 HS_2134_A2_H02_T7C CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=2134 Col=4 Row=O, DNA sequence.    48   0.61   1
EK255242|EK255242.1 1095462007001 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061005733670 5', genomic survey sequence.    48   0.61   1
EJ231564|EJ231564.1 1092402624537 Global-Ocean-Sampling_GS-27-01-01-1P8-2P0KB marine metagenome genomic clone 1061001523913 3', genomic survey sequence.    48   0.61   1
EJ187329|EJ187329.1 1092344160752 Global-Ocean-Sampling_GS-27-01-01-1P8-2P0KB marine metagenome genomic clone 1061001442121 3', genomic survey sequence.    48   0.61   1
EF565095|EF565095.1 Homo sapiens chromosome 8 isolate HA_003160 mRNA sequence.    48   0.61   1
AC007991|AC007991.7 Homo sapiens, clone RP11-44K6, complete sequence.    48   0.61   1
dna update 2007. 9. 2
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q8I7T3) RecName: Full=Substrate-adhesion molecule; Flags: Precu...   387   e-106
CP000463_2697(CP000463|pid:none) Rhodopseudomonas palustris BisA...    39   0.36
AE014134_1481(AE014134|pid:none) Drosophila melanogaster chromos...    36   3.0
CP001037_3074(CP001037|pid:none) Nostoc punctiforme PCC 73102, c...    35   4.0
CP001108_391(CP001108|pid:none) Prosthecochloris aestuarii DSM 2...    35   6.8
FJ528584_1(FJ528584|pid:none) Solenopsis invicta virus 3 isolate...    34   8.8
protein update 2009. 7. 2
PSORT 

 psg: 0.98  gvh: 0.62  alm: 0.22  top: 0.13  tms: 0.13  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: endoplasmic reticulum
	 28.0 %: mitochondrial
	 12.0 %: plasma membrane
	  8.0 %: nuclear
	  8.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for VHE860 is end
5' end seq. ID VHE860F
5' end seq. 
>VHE860F.Seq
AATGAAATCACAAAAAATAGGATCAATGATTCTATTAATTGGAATATTATTAGCAATTTT
TAATTTTGCTTATTCAGATGATGACATAGAACGTTTTTCAATCAATCCAGAAAAACCAAT
TTCCTTCACATCAGATCAACCAGGTTTTCCAACTTCAGCAGATTTCCCAATTGGTTCAAT
TCTAGCAAATTCTTTTTATAGTTTTGGTGGAGATGTAAATTACTTTCAATTAAATATAAG
TTTAATGGAAGAATTTTCAAAAGATGGAAACACAGGCAGCCAAGCAACATGGAATCAATA
TACTTCAGTTCCAGTTTCTCCAATTTCATCAGCCGTTACTGCAAACCGTGTTTACACAAT
GTCAATTGGTTCCCTCAACAGAGTTAAAAAAGGTGATATTACTTCAATGGAATCCACAGA
CTTTCTCAATGATGAAAAATACAGTTCACTTGTAACTACTCTTAATGGTGGTGTATCATA
TGGTGATGACGTTTTCTTTTTATCCTCAAAGAGTGCTACTGGTGAGGCTGTATTGATTCA
CATTAATGATACTGCTACTGGTACATTTGGTACAAGCAGTTATGATGAAATTTTACTTGA
TGCTGCAATTAATGATCCANNNNNNNNNN
Length of 5' end seq. 629
3' end seq. ID VHE860Z
3' end seq. 
>VHE860Z.Seq
NNNNNNNNNNTTTAAACAAAGATTTGGCTGTCTCTACGTTCANTACNAACCAAAGACNTG
ATCATCGNTTCGNTGTTTTCATGTTTATCAAGAGATTCATTATGGCCGTTATCANTGGTA
TCTTATCATTCAAACCAATGACCANNCTATCCATTAACTGGTACTGATTTAGCCGTTCCA
ATCGTTCAAGTAGTTGTCATTGATATCGCTATCATTGGNTATGCTGTATTATTATTCATC
CGTAAACCATATTTCGATCATTATCAATTATGGCTCGAATATATCTTAACTGCCATCAAT
ATTGTTACANTTTATATNTCANTCACTCACATCAAATCACCAAGCGCCGCTGGTGAATTA
ATTGCATGTCTTATTCAAGCTTTAGCCNTTGTAGCTTGTATTGCCGCTTACGTCGTAGCA
NGGCTCCAAATGAGATCTTCTTTCATTAAAAAAGTTAAGAAATATCTCTGCTGTTGTTGT
AAATCCTCAAAATCTTCAGGGAAATCGATTTATCTAAGAAATAAAGAATTAAAAATTTAT
CAATAATAANAATAA
Length of 3' end seq. 555
Connected seq. ID VHE860P
Connected seq. 
>VHE860P.Seq
AATGAAATCACAAAAAATAGGATCAATGATTCTATTAATTGGAATATTATTAGCAATTTT
TAATTTTGCTTATTCAGATGATGACATAGAACGTTTTTCAATCAATCCAGAAAAACCAAT
TTCCTTCACATCAGATCAACCAGGTTTTCCAACTTCAGCAGATTTCCCAATTGGTTCAAT
TCTAGCAAATTCTTTTTATAGTTTTGGTGGAGATGTAAATTACTTTCAATTAAATATAAG
TTTAATGGAAGAATTTTCAAAAGATGGAAACACAGGCAGCCAAGCAACATGGAATCAATA
TACTTCAGTTCCAGTTTCTCCAATTTCATCAGCCGTTACTGCAAACCGTGTTTACACAAT
GTCAATTGGTTCCCTCAACAGAGTTAAAAAAGGTGATATTACTTCAATGGAATCCACAGA
CTTTCTCAATGATGAAAAATACAGTTCACTTGTAACTACTCTTAATGGTGGTGTATCATA
TGGTGATGACGTTTTCTTTTTATCCTCAAAGAGTGCTACTGGTGAGGCTGTATTGATTCA
CATTAATGATACTGCTACTGGTACATTTGGTACAAGCAGTTATGATGAAATTTTACTTGA
TGCTGCAATTAATGATCCA----------TTTAAACAAAGATTTGGCTGTCTCTACGTTC
ANTACNAACCAAAGACNTGATCATCGNTTCGNTGTTTTCATGTTTATCAAGAGATTCATT
ATGGCCGTTATCANTGGTATCTTATCATTCAAACCAATGACCANNCTATCCATTAACTGG
TACTGATTTAGCCGTTCCAATCGTTCAAGTAGTTGTCATTGATATCGCTATCATTGGNTA
TGCTGTATTATTATTCATCCGTAAACCATATTTCGATCATTATCAATTATGGCTCGAATA
TATCTTAACTGCCATCAATATTGTTACANTTTATATNTCANTCACTCACATCAAATCACC
AAGCGCCGCTGGTGAATTAATTGCATGTCTTATTCAAGCTTTAGCCNTTGTAGCTTGTAT
TGCCGCTTACGTCGTAGCANGGCTCCAAATGAGATCTTCTTTCATTAAAAAAGTTAAGAA
ATATCTCTGCTGTTGTTGTAAATCCTCAAAATCTTCAGGGAAATCGATTTATCTAAGAAA
TAAAGAATTAAAAATTTATCAATAATAANAATAA
Length of connected seq. 1164
Full length Seq ID -
Full length Seq. -
Length of full length seq. -