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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE833
Library VH
(Link to library)
Clone ID VHE833
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHE833P
(Link to Original site)
Representative DNA sequence 
>VHE833 (VHE833Q) /CSM/VH/VHE8-B/VHE833Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATXXXXXXXXXXGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAA
GANTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAAC
ATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGA
GGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTANTCATATGCTGATGAATTAACTGC
CTCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTAT
TGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCG
TATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATCNTTCGGTTT
CACCGATGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTNTTCGATCA
TTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCAC
CAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACT
CTAAACAATTTAAGTCGCACTTNAGTTTTCAAAG
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTED---

---VNVTKGVQYLNEIKXSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGG
QIIPTARRVXIC**inclpxylrtnllsrnycprkrywwylxctqss*rycyw*rtsyrf
piiicqssftsirixrfhr*ftfphcrssfptmcxrslgfnwccqqr*esh*srfshqke
krfsprnprs*qiprktlnnlsrtxvfk
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlk---

---VNVTKGVQYLNEIKXSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGG
QIIPTARRVXIC**inclpxylrtnllsrnycprkrywwylxctqss*rycyw*rtsyrf
piiicqssftsirixrfhr*ftfphcrssfptmcxrslgfnwccqqr*esh*srfshqke
krfsprnprs*qiprktlnnlsrtxvfk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r---

---smspkvfni*mklkxlslvlsngppkkvlfvmkt*evsvsifmmspstlmpstevvv
kssqllvvxsyadeltaspxilepiylveitapenaiggixsvlnrrrgivigeerrigs
pllsvkahlpvlesfgftddlrshtagqafpqcvfdhwasigvvnkdkkatevalatrkr
kglapeipdldkfhekl*ti*valxfsk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTED---

---qchqrcsife*n*rxfrwcfpmghqrrccl**kherypfqsl*chpph*chpqrwws
nhpncsscxhmlmn*lppqxs*nqst**kllpqktllvvfxlysivvevlllvknvvsvp
hyylskliyqy*nxsvspmiyvptlpvklshnvxsiiglqlvlstkirkplksl*ppere
kv*pqksqiltnstknskqfkshxsfq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE833 (VHE833Q) /CSM/VH/VHE8-B/VHE833Q.Seq.d/                       2305   0.0
VHF660 (VHF660Q) /CSM/VH/VHF6-C/VHF660Q.Seq.d/                       1179   0.0
VHF772 (VHF772Q) /CSM/VH/VHF7-C/VHF772Q.Seq.d/                       1172   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                       1166   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                       1166   0.0
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                       1166   0.0
VHQ469 (VHQ469Q) /CSM/VH/VHQ4-C/VHQ469Q.Seq.d/                       1166   0.0
VHQ463 (VHQ463Q) /CSM/VH/VHQ4-C/VHQ463Q.Seq.d/                       1166   0.0
VHQ453 (VHQ453Q) /CSM/VH/VHQ4-C/VHQ453Q.Seq.d/                       1166   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.  1164   0.0    1
AF107291|AF107291.1 Candida parapsilosis elongation factor 2 (EFT2) gene, partial cds.    72   2e-61  12
AF107286|AF107286.1 Candida albicans elongation factor 2 (EFT2) gene, partial cds.    78   3e-61  8
Y09664|Y09664.1 C.albicans EFT2 gene.                              78   1e-60  8
AF107289|AF107289.1 Candida tropicalis elongation factor 2 (EFT2) gene, partial cds.    64   1e-56  9
AF534908|AF534908.2 Tetrahymena thermophila elongation factor 2 gene, complete cds.    84   6e-41  5
EH015431|EH015431.1 USDA-FP_188032 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt081_G08 5', mRNA sequence.    78   1e-40  4
EH012247|EH012247.1 USDA-FP_185167 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt044_H07 5', mRNA sequence.    78   1e-40  4
AF107290|AF107290.1 Candida lusitaniae elongation factor 2 (EFT2) gene, partial cds.    64   7e-39  6
AY144999|AY144999.1 Saccharomyces kluyveri clone Contig1706 EFT2 gene, complete cds.    78   1e-37  6
dna update 2007. 9. 2
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   264   6e-69
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   259   1e-67
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   258   3e-67
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   258   4e-67
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   257   7e-67
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   257   7e-67
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   256   2e-66
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   254   3e-66
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   254   3e-66
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   254   4e-66
protein update 2009. 7. 2
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHE833 is cyt
5' end seq. ID VHE833F
5' end seq. 
>VHE833F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATNNNNNNNNNN
Length of 5' end seq. 618
3' end seq. ID VHE833Z
3' end seq. 
>VHE833Z.Seq
NNNNNNNNNNGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGANTCTTT
CGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGG
TATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGG
TCAAATCATCCCAACTGCTCGTCGTGTANTCATATGCTGATGAATTAACTGCCTCCCCAA
NTATCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTA
TTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTT
CCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATCNTTCGGTTTCACCGATG
ATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTNTTCGATCATTGGGCTT
CAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGA
GAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAA
TTTAAGTCGCACTTNAGTTTTCAAAG
Length of 3' end seq. 626
Connected seq. ID VHE833P
Connected seq. 
>VHE833P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGAT----------GTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAA
GANTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAAC
ATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGA
GGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTANTCATATGCTGATGAATTAACTGC
CTCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTAT
TGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCG
TATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATCNTTCGGTTT
CACCGATGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTNTTCGATCA
TTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCAC
CAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACT
CTAAACAATTTAAGTCGCACTTNAGTTTTCAAAG
Length of connected seq. 1224
Full length Seq ID -
Full length Seq. -
Length of full length seq. -