VHE814 [Dicty_cDB - Dictyostelium discoideum cDNA Clone Overview]

VHE814
Library	VH (Link to library)
Clone ID	VHE814 (Link to dictyBase)
Atlas ID	-
NBRP ID	-
dictyBase ID	-
Link to Contig	Contig-U16507-1
Original site URL	http://dictycdb.biol.tsukuba.ac.jp/CSM/VH/VHE8-A/VHE814Q.Seq.d/
Representative seq. ID	VHE814P (Link to Original site)
Representative DNA sequence	>VHE814 (VHE814Q) /CSM/VH/VHE8-A/VHE814Q.Seq.d/ ACATTATTAAATGTTCATATTGTTGCTCATACTCATGATGATGTTGGTTGGTTAAAAACA GTTGATGAATATTATTATGGATCAAATATGTCAATTGCATTTGCAGGTGTTCAATATACT TTGGATACAGCCATTACCTGTTTATTGGCAAATCCAGAAAGAAAGTTTATTTATGTTGAA ATTGCATTTTTCCAAAGATGGTGGGATGAACAAAGTACAACAATGCAAAATATTGTTAAG GGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAATGGTGGGTATTGTATGAATGATGAG GCTACTACCTACTATGACGATACTATTGACCAAATGACATTGGGCCATCAATTCCTTTGG GAGAATTTCGGAGTTATGCCAAAGATAGGCTGGCACATAGATCCATTTGGCCATAGTGCA ACACAAGCTCGTATCTTTGGTCAATTAGGATTCGATGCATTCATTATCGGTCGTATGGAT TATCAAGATATCGAAGCTCGTTTAGAGAATAAGCAAATGGAGTTCATGTGGAGATCGACC CAAAGTACACCAGAGAATCAAGTTTTCACATCAGTACTACGTGCAATGTATTGTACTCCA GXXXXXXXXXXCAACCAGTGGTAATTATGTACNTGTGAATGCTATTGCTTACATTCAAGA TCTTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTT AAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGG TGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCA TGATATTTCAAGTTATGCTCAATCTCATTATCGTCNTGCTGCACTNTCTTTATCACATCC ATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCA AGGTGTTTACTCACCATTGACTTCAGCNTCACNTTTACCAAATGGTTTAAAGATTCAAAC TCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCA AATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTT TTCAACAATTACAATCACTTNTGCGACTGAAATGAATTTAACTGGTGTTCAAAAATTATC AAATTTATCAAG
sequence update	2002.10.25
Translated Amino Acid sequence	TLLNVHIVAHTHDDVGWLKTVDEYYYGSNMSIAFAGVQYTLDTAITCLLANPERKFIYVE IAFFQRWWDEQSTTMQNIVKGLVESGQLEFINGGYCMNDEATTYYDDTIDQMTLGHQFLW ENFGVMPKIGWHIDPFGHSATQARIFGQLGFDAFIIGRMDYQDIEARLENKQMEFMWRST QSTPENQVFTSVLRAMYCTP--- ---TSGNYVXVNAIAYIQDLNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDGRGV GQPMNESTQIVTTSKLIFHDISSYAQSHYRXAALSLSHPLLPMFTTTQQSSNDWNSQYQG VYSPLTSASXLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLSTIFS TITITXATEMNLTGVQKLSNLS
Translated Amino Acid sequence (All Frames)	Frame A: TLLNVHIVAHTHDDVGWLKTVDEYYYGSNMSIAFAGVQYTLDTAITCLLANPERKFIYVE IAFFQRWWDEQSTTMQNIVKGLVESGQLEFINGGYCMNDEATTYYDDTIDQMTLGHQFLW ENFGVMPKIGWHIDPFGHSATQARIFGQLGFDAFIIGRMDYQDIEARLENKQMEFMWRST QSTPENQVFTSVLRAMYCTP--- ---qpvvimyxmllltfkilinhynsplslievvdvhremvnwi*civvlkmmvvv lvnqmsqlkslqlvnfsmifqvmlnliivxlhxlyhihysqclqplnnlqtigilnik vfthhlqxhxyqmvrfklyngiikiipyfyvlkifiklmvkihkihklslifqlsf qqlqslxrlkilvfknyqiyq Frame B: hymfilllilmmmlvgkqlmniimdqicqlhlqvfnilwiqplpvywqiqkeslfmlk lhfskdggmnkvqqckillrvwlkvvnwnsmvgivmmrllptmtilltkhwainsfg riselcqragtihlaivqhklvslvndsmhslsvvwiikisklvriskwsscgdrp kvhqrikfshqyyvqcivlq--- ---nqwlctcecyclhsrssiitihhchkswmcivkrwsigyddassyferwswc wstnevnsnrynidfpyfklcsislsscctxfitsitpnvynhstifkrlefsisr clltidfsxtftkwfkdsnstmvrsryhtftykylsnwsrftrstnyhfrsfnylf nnynhxcdnefnwcskiikfik Frame C: iikcsyccsyscwlvknsillwikyvncicrcsiyfgyshylfigksrkkvylcn cifpkmvgtkynnakycgfgkwsigihkwwvlyegyyllryypndigpsiplg efrsyakdrlahrsiwpcntssylwsirircihyrsyglsryrssfre*angvhveidp kytressfhisttcnvlys--- ---TSGNYVXVNAIAYIQDLNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDGRGV GQPMNESTQIVTTSKLIFHDISSYAQSHYRXAALSLSHPLLPMFTTTQQSSNDWNSQYQG VYSPLTSASXLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLSTIFS TITITXATEMNLTGVQKLSNLS
Homology vs CSM-cDNA	Score E Sequences producing significant alignments: (bits) Value VHE814 (VHE814Q) /CSM/VH/VHE8-A/VHE814Q.Seq.d/ 2307 0.0 AFF321 (AFF321Q) /CSM/AF/AFF3-A/AFF321Q.Seq.d/ 1191 0.0 VHL504 (VHL504Q) /CSM/VH/VHL5-A/VHL504Q.Seq.d/ 1189 0.0 SHK692 (SHK692Q) /CSM/SH/SHK6-D/SHK692Q.Seq.d/ 1185 0.0 VHG749 (VHG749Q) /CSM/VH/VHG7-C/VHG749Q.Seq.d/ 1178 0.0 SHC431 (SHC431Q) /CSM/SH/SHC4-B/SHC431Q.Seq.d/ 1158 0.0 VHE761 (VHE761Q) /CSM/VH/VHE7-C/VHE761Q.Seq.d/ 1148 0.0 CHQ142 (CHQ142Q) /CSM/CH/CHQ1-B/CHQ142Q.Seq.d/ 1148 0.0 CHJ182 (CHJ182Q) /CSM/CH/CHJ1-D/CHJ182Q.Seq.d/ 1148 0.0 VHM180 (VHM180Q) /CSM/VH/VHM1-D/VHM180Q.Seq.d/ 1146 0.0
own update	2004.12.24
Homology vs DNA	Score E Sequences producing significant alignments: (bits) Value N M82822\|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds. 1108 0.0 1 CK149800\|CK149800.1 CsmgEST00336 Culicoides sonorensis female serum-fed midgut cDNA Library Culicoides sonorensis cDNA, mRNA sequence. 117 1e-23 3 DN814237\|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence. 52 1e-16 4 DN816506\|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence. 52 1e-16 4 CX633815\|CX633815.1 taj44a03.y3 Hydra EST UCI 5 Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence. 52 2e-16 4 DN816232\| truncated lysosomal acid alpha-mannosidase [Homo sapiens], mRNA sequence. 52 2e-16 4 DN811862\| lysosomal alpha-mannosidase [Cavia porcellus], mRNA sequence. 52 2e-16 4 DT613126\|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence. 52 2e-16 4 DN815247\| lysosomal alpha-mannosidase [Cavia porcellus], mRNA sequence. 52 3e-16 4 CB073433\|CB073433.1 taa32h01.y1 Hydra EST -II Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence. 52 2e-15 4
dna update	2007. 9. 2
Homology vs Protein	Score E Sequences producing significant alignments: (bits) Value (P34098) RecName: Full=Lysosomal alpha-mannosidase; Sho... 423 e-117 M82822_1(M82822\|pid:none) Dictyostelium discoideum alpha-mannosi... 413 e-114 (Q29451) RecName: Full=Lysosomal alpha-mannosidase; Sho... 244 4e-63 AK291572_1(AK291572\|pid:none) Homo sapiens cDNA FLJ76867 complet... 237 5e-61 U05572_1(U05572\|pid:none) Human lysosomal alpha-mannosidase (MAN... 237 5e-61 BC091843_1(BC091843\|pid:none) Danio rerio zgc:110815, mRNA (cDNA... 237 5e-61 AB209921_1(AB209921\|pid:none) Homo sapiens mRNA for mannosidase,... 237 5e-61 JC2200(JC2200)alpha-mannosidase (EC 3.2.1.24) precursor - human 236 2e-60 AY099704_1(AY099704\|pid:none) Arabidopsis thaliana alpha-mannosi... 235 2e-60 AB005230_20(AB005230\|pid:none) Arabidopsis thaliana genomic DNA,... 235 2e-60
protein update	2009. 7. 1
PSORT	psg: 0.83 gvh: 0.41 alm: 0.41 top: 0.53 tms: 0.00 mit: 0.18 mip: 0.00 nuc: 0.00 erl: 0.00 erm: 0.00 pox: 0.00 px2: 0.00 vac: 0.00 rnp: 0.00 act: 0.00 caa: 0.00 yqr: 0.00 tyr: 0.00 leu: 0.00 gpi: 0.00 myr: 0.00 dna: 0.00 rib: 0.00 bac: 0.00 m1a: 0.00 m1b: 0.00 m2 : 0.00 mNt: 0.00 m3a: 0.00 m3b: 0.00 m_ : 1.00 56.0 %: cytoplasmic 32.0 %: nuclear 4.0 %: cytoskeletal 4.0 %: vesicles of secretory system 4.0 %: endoplasmic reticulum >> prediction for VHE814 is cyt
5' end seq. ID	VHE814F
5' end seq.	>VHE814F.Seq ACATTATTAAATGTTCATATTGTTGCTCATACTCATGATGATGTTGGTTGGTTAAAAACA GTTGATGAATATTATTATGGATCAAATATGTCAATTGCATTTGCAGGTGTTCAATATACT TTGGATACAGCCATTACCTGTTTATTGGCAAATCCAGAAAGAAAGTTTATTTATGTTGAA ATTGCATTTTTCCAAAGATGGTGGGATGAACAAAGTACAACAATGCAAAATATTGTTAAG GGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAATGGTGGGTATTGTATGAATGATGAG GCTACTACCTACTATGACGATACTATTGACCAAATGACATTGGGCCATCAATTCCTTTGG GAGAATTTCGGAGTTATGCCAAAGATAGGCTGGCACATAGATCCATTTGGCCATAGTGCA ACACAAGCTCGTATCTTTGGTCAATTAGGATTCGATGCATTCATTATCGGTCGTATGGAT TATCAAGATATCGAAGCTCGTTTAGAGAATAAGCAAATGGAGTTCATGTGGAGATCGACC CAAAGTACACCAGAGAATCAAGTTTTCACATCAGTACTACGTGCAATGTATTGTACTCCA GNNNNNNNNNN
Length of 5' end seq.	611
3' end seq. ID	VHE814Z
3' end seq.	>VHE814Z.Seq NNNNNNNNNNCAACCAGTGGTAATTATGTACNTGTGAATGCTATTGCTTACATTCAAGAT CTTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTA AGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGT GTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCAT GATATTTCAAGTTATGCTCAATCTCATTATCGTCNTGCTGCACTNTCTTTATCACATCCA TTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAA GGTGTTTACTCACCATTGACTTCAGCNTCACNTTTACCAAATGGTTTAAAGATTCAAACT CTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAA ATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTT TCAACAATTACAATCACTTNTGCGACTGAAATGAATTTAACTGGTGTTCAAAAATTATCA AATTTATCAAG
Length of 3' end seq.	611
Connected seq. ID	VHE814P
Connected seq.	>VHE814P.Seq ACATTATTAAATGTTCATATTGTTGCTCATACTCATGATGATGTTGGTTGGTTAAAAACA GTTGATGAATATTATTATGGATCAAATATGTCAATTGCATTTGCAGGTGTTCAATATACT TTGGATACAGCCATTACCTGTTTATTGGCAAATCCAGAAAGAAAGTTTATTTATGTTGAA ATTGCATTTTTCCAAAGATGGTGGGATGAACAAAGTACAACAATGCAAAATATTGTTAAG GGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAATGGTGGGTATTGTATGAATGATGAG GCTACTACCTACTATGACGATACTATTGACCAAATGACATTGGGCCATCAATTCCTTTGG GAGAATTTCGGAGTTATGCCAAAGATAGGCTGGCACATAGATCCATTTGGCCATAGTGCA ACACAAGCTCGTATCTTTGGTCAATTAGGATTCGATGCATTCATTATCGGTCGTATGGAT TATCAAGATATCGAAGCTCGTTTAGAGAATAAGCAAATGGAGTTCATGTGGAGATCGACC CAAAGTACACCAGAGAATCAAGTTTTCACATCAGTACTACGTGCAATGTATTGTACTCCA G----------CAACCAGTGGTAATTATGTACNTGTGAATGCTATTGCTTACATTCAAGA TCTTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTT AAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGG TGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCA TGATATTTCAAGTTATGCTCAATCTCATTATCGTCNTGCTGCACTNTCTTTATCACATCC ATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCA AGGTGTTTACTCACCATTGACTTCAGCNTCACNTTTACCAAATGGTTTAAAGATTCAAAC TCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCA AATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTT TTCAACAATTACAATCACTTNTGCGACTGAAATGAATTTAACTGGTGTTCAAAAATTATC AAATTTATCAAG
Length of connected seq.	1202
Full length Seq ID	-
Full length Seq.	-
Length of full length seq.	-

Dicty_cDB - Dictyostelium discoideum cDNA Clone Overview

Dicty_cDB

Atlas

Dicty_cDB - Dictyostelium discoideum cDNA Clone Overview

Dicty_cDB

Atlas