>VHE809 (VHE809Q) /CSM/VH/VHE8-A/VHE809Q.Seq.d/
TGGCCTACTGGAAAAAAAAGCCACACACACATAAAAATGGTAATAAAAAAATTATTTATA
TTAATTTTTTGTTTGTTTTTAATTATCAATGAAATTAATGAAAAAAAAACAAAAATAAAT
GATATTAAAAAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCATATTGTTGCT
CATACTCATGATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTATGGATCAAAT
ATGTCAATTGCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACCTGTTTATTG
GCAAATCCAGAAAGAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGAT
GAACAAAGTACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAA
TTCATAAATGGTGGGTATTGTATGAATGATGAGGCTACTACCTACTATGACGATACTATT
GACCAAATGACATTGGGCCATCAATTCCTTTGGGAGAATTTCGGAGTTATGCCAAAGATA
GGCTGGCACATANATCCATTTGGCCATAGTGCAACACAAGCTCGTATCTTTGGTCAATXX
XXXXXXXXAACCAGTGGTAATTATGTACNTGTGAATGCTATTGCTTACATTCAAGATCTT
AATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGNTAAGA
GATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTT
GGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCATGAT
ATTTCAAGTTATGCTCAATCTCATTATCGTCNTGCTGCACTNTCTTTATCACATCCATTA
CTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAAGGT
GTTTACTCACCATTGACTTCAGCNTCACNTTTACCAAATGGTTTAAAGATTCAAACTCTA
CAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAAATT
GATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTTTCA
ACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCAAAT
TTATCAAG

WPTGKKSHTHIKMVIKKLFILIFCLFLIINEINEKKTKINDIKKSKPKLSSTLLNVHIVA
HTHDDVGWLKTVDEYYYGSNMSIAFAGVQYTLDTAITCLLANPERKFIYVEIAFFQRWWD
EQSTTMQNIVKGLVESGQLEFINGGYCMNDEATTYYDDTIDQMTLGHQFLWENFGVMPKI
GWHIXPFGHSATQARIFGQ---

---TSGNYVXVNAIAYIQDLNQSLQFTIVTDRSRGCASXRDGQLDMMMHRRTLKDDGRGV
GQPMNESTQIVTTSKLIFHDISSYAQSHYRXAALSLSHPLLPMFTTTQQSSNDWNSQYQG
VYSPLTSASXLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLSTIFS
TITITSATEMNLTGVQKLSNLS

Frame A:
WPTGKKSHTHIKMVIKKLFILIFCLFLIINEINEKKTKINDIKKSKPKLSSTLLNVHIVA
HTHDDVGWLKTVDEYYYGSNMSIAFAGVQYTLDTAITCLLANPERKFIYVEIAFFQRWWD
EQSTTMQNIVKGLVESGQLEFINGGYCMNDEATTYYDDTIDQMTLGHQFLWENFGVMPKI
GWHIXPFGHSATQARIFGQ---

---nqw*lctcecyclhsrs*siitihhch**kswmcixkrwsigyddassyfer*wswc
wstne*vnsnryn**idfp*yfklcsislsscctxfitsitpnvynhstifkrlefsisr
clltidfsxtftkwfkdsnstmvr*sr*yhtfty*kylsn*wsrftrstnyhfrsfnylf
nnynhfcn*nefnwcskiikfik

Frame B:
gllekkatht*kw**knyly*ffvcf*lsmklmkkkqk*milknqnqnyhqhy*mfilll
ilmmmlvg*kqlmniimdqicqlhlqvfnilwiqplpvywqiqkeslfmlklhfskdggm
nkvqqckillrvwlkvvnwns*mvgiv*mmrllptmtilltk*hwainsfgriselcqr*
agtxihlaivqhklvslvn---

---TSGNYVXVNAIAYIQDLNQSLQFTIVTDRSRGCASXRDGQLDMMMHRRTLKDDGRGV
GQPMNESTQIVTTSKLIFHDISSYAQSHYRXAALSLSHPLLPMFTTTQQSSNDWNSQYQG
VYSPLTSASXLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLSTIFS
TITITSATEMNLTGVQKLSNLS

Frame C:
aywkkkphthkngnkkiiyinflfvfnyq*n**kknknk*y*kiktktiiniikcsyccs
ys**cwlvkns**illwikyvncicrcsiyfgyshylfigksrkkvylc*ncifpkmvg*
tkynnakyc*gfg*kwsigihkwwvlye**gyyll*ryy*pndigpsiplgefrsyakdr
lahxsiwp*cntssylws---

---pvvimyx*mllltfkilinhynsplslievvdvhr*emvnwi**civvl*kmmvvvl
vnq*msqlkslqlvn*fsmifqvmlnliivxlhxlyhihysqclqplnnlqtigilnikv
fthh*lqxhxyqmv*rfklyng*iikiipyfyvlkifiklmvkihkihklsl*ifqlsfq
qlqsllqlk*i*lvfknyqiyq

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE809 (VHE809Q) /CSM/VH/VHE8-A/VHE809Q.Seq.d/                       2008   0.0
VHE761 (VHE761Q) /CSM/VH/VHE7-C/VHE761Q.Seq.d/                       1154   0.0
VHE586 (VHE586Q) /CSM/VH/VHE5-D/VHE586Q.Seq.d/                       1154   0.0
CHQ142 (CHQ142Q) /CSM/CH/CHQ1-B/CHQ142Q.Seq.d/                       1154   0.0
CHJ182 (CHJ182Q) /CSM/CH/CHJ1-D/CHJ182Q.Seq.d/                       1154   0.0
VHE609 (VHE609Q) /CSM/VH/VHE6-A/VHE609Q.Seq.d/                       1152   0.0
VHE836 (VHE836Q) /CSM/VH/VHE8-B/VHE836Q.Seq.d/                       1148   0.0
VHE741 (VHE741Q) /CSM/VH/VHE7-B/VHE741Q.Seq.d/                       1148   0.0
SHF540 (SHF540Q) /CSM/SH/SHF5-B/SHF540Q.Seq.d/                       1148   0.0
SHF209 (SHF209Q) /CSM/SH/SHF2-A/SHF209Q.Seq.d/                       1148   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1114   0.0    1
CK149800|CK149800.1 CsmgEST00336 Culicoides sonorensis female serum-fed midgut cDNA Library Culicoides sonorensis cDNA, mRNA sequence.   117   1e-23  3
CB073433|CB073433.1 taa32h01.y1 Hydra EST -II Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence.    52   2e-13  3
DN814237|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   3e-13  3
DN816506|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   3e-13  3
CV682907|CV682907.1 service43_H06_sjs2-16-2B11-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    74   3e-13  2
CX633815|CX633815.1 taj44a03.y3 Hydra EST UCI 5 Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence.    52   4e-13  3
CV688215|CV688215.1 sjs2-16-2_G01_sjs2-16-2G01-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    74   4e-13  2
CV682854|CV682854.1 service30_G09_sjs2-14-10C09-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    74   4e-13  2
DN816232| truncated lysosomal acid alpha-mannosidase [Homo sapiens], mRNA sequence.    52   4e-13  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   317   4e-85
(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   317   4e-85
AK068415_1(AK068415|pid:none) Oryza sativa Japonica Group cDNA c...   198   3e-49
AY099704_1(AY099704|pid:none) Arabidopsis thaliana alpha-mannosi...   196   1e-48
AB005230_20(AB005230|pid:none) Arabidopsis thaliana genomic DNA,...   196   1e-48
AB026648_12(AB026648|pid:none) Arabidopsis thaliana genomic DNA,...   196   1e-48
(Q29451) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   192   2e-47
AK291572_1(AK291572|pid:none) Homo sapiens cDNA FLJ76867 complet...   191   6e-47
U05572_1(U05572|pid:none) Human lysosomal alpha-mannosidase (MAN...   191   6e-47
AB209921_1(AB209921|pid:none) Homo sapiens mRNA for mannosidase,...   191   6e-47

 psg: 0.71  gvh: 0.51  alm: 0.17  top: 0.27  tms: 0.07  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: endoplasmic reticulum
	 20.0 %: mitochondrial
	 16.0 %: cytoplasmic
	 16.0 %: nuclear
	 12.0 %: Golgi
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VHE809 is end

>VHE809F.Seq
TGGCCTACTGGAAAAAAAAGCCACACACACATAAAAATGGTAATAAAAAAATTATTTATA
TTAATTTTTTGTTTGTTTTTAATTATCAATGAAATTAATGAAAAAAAAACAAAAATAAAT
GATATTAAAAAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCATATTGTTGCT
CATACTCATGATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTATGGATCAAAT
ATGTCAATTGCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACCTGTTTATTG
GCAAATCCAGAAAGAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGAT
GAACAAAGTACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAA
TTCATAAATGGTGGGTATTGTATGAATGATGAGGCTACTACCTACTATGACGATACTATT
GACCAAATGACATTGGGCCATCAATTCCTTTGGGAGAATTTCGGAGTTATGCCAAAGATA
GGCTGGCACATANATCCATTTGGCCATAGTGCAACACAAGCTCGTATCTTTGGTCAATNN
NNNNNNNN

>VHE809Z.Seq
NNNNNNNNNNAACCAGTGGTAATTATGTACNTGTGAATGCTATTGCTTACATTCAAGATC
TTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGNTAA
GAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGTG
TTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCATG
ATATTTCAAGTTATGCTCAATCTCATTATCGTCNTGCTGCACTNTCTTTATCACATCCAT
TACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAAG
GTGTTTACTCACCATTGACTTCAGCNTCACNTTTACCAAATGGTTTAAAGATTCAAACTC
TACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAAA
TTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTTT
CAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCAA
ATTTATCAAG

>VHE809P.Seq
TGGCCTACTGGAAAAAAAAGCCACACACACATAAAAATGGTAATAAAAAAATTATTTATA
TTAATTTTTTGTTTGTTTTTAATTATCAATGAAATTAATGAAAAAAAAACAAAAATAAAT
GATATTAAAAAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCATATTGTTGCT
CATACTCATGATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTATGGATCAAAT
ATGTCAATTGCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACCTGTTTATTG
GCAAATCCAGAAAGAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGAT
GAACAAAGTACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAA
TTCATAAATGGTGGGTATTGTATGAATGATGAGGCTACTACCTACTATGACGATACTATT
GACCAAATGACATTGGGCCATCAATTCCTTTGGGAGAATTTCGGAGTTATGCCAAAGATA
GGCTGGCACATANATCCATTTGGCCATAGTGCAACACAAGCTCGTATCTTTGGTCAAT--
--------AACCAGTGGTAATTATGTACNTGTGAATGCTATTGCTTACATTCAAGATCTT
AATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGNTAAGA
GATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTT
GGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCATGAT
ATTTCAAGTTATGCTCAATCTCATTATCGTCNTGCTGCACTNTCTTTATCACATCCATTA
CTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAAGGT
GTTTACTCACCATTGACTTCAGCNTCACNTTTACCAAATGGTTTAAAGATTCAAACTCTA
CAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAAATT
GATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTTTCA
ACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCAAAT
TTATCAAG