VHE802 [Dicty_cDB - Dictyostelium discoideum cDNA Clone Overview]

VHE802
Library	VH (Link to library)
Clone ID	VHE802 (Link to dictyBase)
Atlas ID	-
NBRP ID	-
dictyBase ID	-
Link to Contig	Contig-U16260-1
Original site URL	http://dictycdb.biol.tsukuba.ac.jp/CSM/VH/VHE8-A/VHE802Q.Seq.d/
Representative seq. ID	VHE802P (Link to Original site)
Representative DNA sequence	>VHE802 (VHE802Q) /CSM/VH/VHE8-A/VHE802Q.Seq.d/ AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC ACGGTAAAGCCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCCXXXXXXXXXXCCACGAATGGGAT AAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAATNTCCAT CGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGC TTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTT CAATCTTTATGATGTCACCNTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCAT CCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTATCTTAGA ACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGT ACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATT ATCTGTCAAAGCTCATTTACCAGTATTAGAATCNTTCGGTTTCACCGATGATTTACGTTC CCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGT TGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTT AGCCCCAGAAATCCCAGATCTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCA CTTGAGTTTTCAAAG
sequence update	2002.10.25
Translated Amino Acid sequence	LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKATLSDSLIQRAGIIADKV SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE EAYLSFRRAIESVNVIVGNTEDKEFGDVTVS--- ---HEWDKNDAMNIWSFGPEGNGANXHRQCHQRCSIFEnrffrwcfpmghqrrccl** kherypfqslchxphchpqrwwsnhpncssctlccinclpnylrtnllsrnycprkr ywwyllctqssrycywrtsyrfpiiicqssftsirixrfhrftfphcrssfptmclr slgfnwccqqreshsrfshqkekrfsprnprsdkfhekltivalefsk
Translated Amino Acid sequence (All Frames)	Frame A: nsrvqkkstkwlisqsikfvplwtvvkiseicllllmsitvkplyqilskelvllpik flvidichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnfsilsipqvtlis vqkllpsvllmvlslsiaskvfaykpklyyvkpslnvsnqsysstksivsynsntl kklislsvellnpsmsllvtlkirnsvmslyp--- ---prmgkchehlvfrsrrrcqxpssmspkvfnimklkilslvlsngppkkvlfvm ktevsvsifmmspstlmpstevvvkssqllvvysmllnlppqlsnqstkllpqkt llvvftlysivvevlllvknvvsvphyylskliyqynxsvspmiyvptlpvklshnvss iiglqlvlstkirkplkslpperekvpqksqiqiprktlnnlsrtvfk Frame B: tleckkskvqngfhnrsnschygpskyqkyvcycscrsrshfirffnpkswyycrs fwyeiyvmsctrawyhhificitslrnakgrqitswlyftrifnqsyrfprsrfq frsnccppcywcfsryrlrrrclrtnrncitssrrtyqtslirqqsrsflirtpikh rslslfpsyirqchcwhrgirchcip--- ---HEWDKNDAMNIWSFGPEGNGANXHRQCHQRCSIFEnrffrwcfpmghqrrccl** kherypfqslchxphchpqrwwsnhpncssctlccinclpnylrtnllsrnycprkr ywwyllctqssrycywrtsyrfpiiicqssftsirixrfhrftfphcrssfptmclr slgfnwccqqreshsrfshqkekrfsprnprsdkfhekltivalefsk Frame C: LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKATLSDSLIQRAGIIADKV SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE EAYLSFRRAIESVNVIVGNTEDKEFGDVTVS--- ---tngikmmp*tfglsvqkvtvpxsivnvtkgvqylneikdsfvgafqwatkegvvcde nmrgirfnlydvtxhtdaihrgggqiiptarrvlyaaeltasptilepiylveitapena iggiysvlnrrrgivigeerrigspllsvkahlpvlesfgftddlrshtagqafpqcvfd hwasigvvnkdkkatevalatrkrkglapeipdltnstknskqfkshlsfq
Homology vs CSM-cDNA	Score E Sequences producing significant alignments: (bits) Value VHE802 (VHE802Q) /CSM/VH/VHE8-A/VHE802Q.Seq.d/ 2529 0.0 VHE779 (VHE779Q) /CSM/VH/VHE7-D/VHE779Q.Seq.d/ 2478 0.0 VHE555 (VHE555Q) /CSM/VH/VHE5-C/VHE555Q.Seq.d/ 2470 0.0 VHM782 (VHM782Q) /CSM/VH/VHM7-D/VHM782Q.Seq.d/ 2468 0.0 VHF330 (VHF330Q) /CSM/VH/VHF3-B/VHF330Q.Seq.d/ 1318 0.0 VHF458 (VHF458Q) /CSM/VH/VHF4-C/VHF458Q.Seq.d/ 1312 0.0 VHF149 (VHF149Q) /CSM/VH/VHF1-C/VHF149Q.Seq.d/ 1306 0.0 VHO564 (VHO564Q) /CSM/VH/VHO5-C/VHO564Q.Seq.d/ 1304 0.0 VHK412 (VHK412Q) /CSM/VH/VHK4-A/VHK412Q.Seq.d/ 1304 0.0 VHC423 (VHC423Q) /CSM/VH/VHC4-A/VHC423Q.Seq.d/ 1304 0.0
own update	2009. 4. 4
Homology vs DNA	Score E Sequences producing significant alignments: (bits) Value N M26017\|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds. 1213 0.0 1 AF107291\|AF107291.1 Candida parapsilosis elongation factor 2 (EFT2) gene, partial cds. 72 2e-71 13 AF107286\|AF107286.1 Candida albicans elongation factor 2 (EFT2) gene, partial cds. 78 9e-69 9 Y09664\|Y09664.1 C.albicans EFT2 gene. 78 2e-67 9 AF107289\|AF107289.1 Candida tropicalis elongation factor 2 (EFT2) gene, partial cds. 70 2e-66 10 AF107290\|AF107290.1 Candida lusitaniae elongation factor 2 (EFT2) gene, partial cds. 64 2e-46 7 AY144999\|AY144999.1 Saccharomyces kluyveri clone Contig1706 EFT2 gene, complete cds. 78 1e-45 7 AF534908\|AF534908.2 Tetrahymena thermophila elongation factor 2 gene, complete cds. 84 1e-43 6 AY497659\|AY497659.1 Candida dubliniensis strain CBS 7987 translation elongation factor gene, partial cds. 78 3e-43 5 AR550596\|AR550596.1 Sequence 5727 from patent US 6747137. 78 1e-42 6
dna update	2007. 9. 2
Homology vs Protein	Score E Sequences producing significant alignments: (bits) Value AM270011_29(AM270011\|pid:none) Aspergillus niger contig An02c016... 273 1e-71 AF083031_87(AF083031\|pid:none) Guillardia theta nucleomorph chro... 268 4e-70 (Q6FYA7) RecName: Full=Elongation factor 2; Short=EF-2;... 267 7e-70 DQ357217_1(DQ357217\|pid:none) Leishmania braziliensis elongation... 266 2e-69 AM494972_26(AM494972\|pid:none) Leishmania braziliensis chromosom... 266 2e-69 AF248644_1(AF248644\|pid:none) Filobasidiella neoformans var. neo... 266 2e-69 CT005272_19(CT005272\|pid:none) Leishmania major strain Friedlin,... 265 2e-69 (Q874B9) RecName: Full=Elongation factor 2; Short=EF-2;... 265 3e-69 AB095183_1(AB095183\|pid:none) Cyanidioschyzon merolae gene for e... 263 1e-68 CU638744_366(CU638744\|pid:none) Podospora anserina genomic DNA c... 263 1e-68
protein update	2009. 7. 1
PSORT	psg: 0.71 gvh: 0.38 alm: 0.34 top: 0.53 tms: 0.00 mit: 0.30 mip: 0.00 nuc: 0.00 erl: 0.00 erm: 0.00 pox: 0.00 px2: 0.00 vac: 0.00 rnp: 0.00 act: 0.00 caa: 0.00 yqr: 0.00 tyr: 0.00 leu: 0.00 gpi: 0.00 myr: 0.00 dna: 0.00 rib: 0.00 bac: 0.00 m1a: 0.00 m1b: 0.00 m2 : 0.00 mNt: 0.00 m3a: 0.00 m3b: 0.00 m_ : 1.00 44.0 %: cytoplasmic 32.0 %: nuclear 20.0 %: mitochondrial 4.0 %: vesicles of secretory system >> prediction for VHE802 is cyt
5' end seq. ID	VHE802F
5' end seq.	>VHE802F.Seq AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC ACGGTAAAGCCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCCNNNNNNNNNN
Length of 5' end seq.	647
3' end seq. ID	VHE802Z
3' end seq.	>VHE802Z.Seq NNNNNNNNNNCCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAG AAGGTAACGGTGCCAATNTCCATCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAA ATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGAT GAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCNTCCACACTGATGCCATC CACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTA ACTGCCTCCCCAACTATCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAAC GCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAA CGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATCNTTC GGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTC GATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTA GCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTGACAAATTCCACGAAA AACTCTAAACAATTTAAGTCGCACTTGAGTTTTCAAAG
Length of 3' end seq.	698
Connected seq. ID	VHE802P
Connected seq.	>VHE802P.Seq AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC ACGGTAAAGCCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCC----------CCACGAATGGGAT AAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAATNTCCAT CGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGC TTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTT CAATCTTTATGATGTCACCNTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCAT CCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTATCTTAGA ACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGT ACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATT ATCTGTCAAAGCTCATTTACCAGTATTAGAATCNTTCGGTTTCACCGATGATTTACGTTC CCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGT TGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTT AGCCCCAGAAATCCCAGATCTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCA CTTGAGTTTTCAAAG
Length of connected seq.	1325
Full length Seq ID	-
Full length Seq.	-
Length of full length seq.	-

Dicty_cDB - Dictyostelium discoideum cDNA Clone Overview

Dicty_cDB

Atlas

Dicty_cDB - Dictyostelium discoideum cDNA Clone Overview

Dicty_cDB

Atlas