>VHE761 (VHE761Q) /CSM/VH/VHE7-C/VHE761Q.Seq.d/
TGGCCTACTGGAAAGCCACACACACATAAAAATGGTAATAAAAAAATTATTTATATTAAT
TTTTTGTTTGTTTTTAATTATCAATGAAATTAATGGAAAAAAAAACAAAAATAAATGATA
TTAAAAAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCATATTGTTGCTCATA
CTCATGATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTATGGATCAAATATGT
CAATTGCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACCTGTTTATTGGCAA
ATCCAGAAAGAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGATGAAC
AAAGTACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCA
TAAATGGTGGGTATTGTATGAATGATGAGGCTACTACCTACTATGACGATACTATTGACC
AAATGACATTGGGCCATCAATTCCTTTGGGAGAATTTCGGAGTTATGCCAAAGATAGGCT
GGCACATAGATCCATTTGGCCATAGTGCAACACAAGCTCGTATCTTTGGTCAXXXXXXXX
XXGTCCATCATGGAATTTACCCGTGGTTCAACCAACCAGTGGTAATTATGTACNTGTGAA
TGCTATTGCTTACATTCAAGATCTTAATCAATCATTACAATTCACCATTGTCACTGATAG
AAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTAC
TTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTAC
AACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCNTGC
TGCACTNTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAA
CGATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCNTCACCTTTACC
AAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTT
ACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCAC
TTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTT
AACTGGTGTTCAAAAATTATCAAATTTATCAAG

aywkatht*kw**knyly*ffvcf*LSMKLMEKKTKINDIKKSKPKLSSTLLNVHIVAHT
HDDVGWLKTVDEYYYGSNMSIAFAGVQYTLDTAITCLLANPERKFIYVEIAFFQRWWDEQ
STTMQNIVKGLVESGQLEFINGGYCMNDEATTYYDDTIDQMTLGHQFLWENFGVMPKIGW
HIDPFGHSATQARIFG---

---PSWNLPVVQPTSGNYVXVNAIAYIQDLNQSLQFTIVTDRSRGCASLRDGQLDMMMHR
RTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRXAALSLSHPLLPMFTTTQQS
SNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQT
ITLDLSTIFSTITITSATEMNLTGVQKLSNLS

Frame A:
wptgkphthkngnkkiiyinflfvfnyq*n*wkkkqk*milknqnqnyhqhy*mfillli
lmmmlvg*kqlmniimdqicqlhlqvfnilwiqplpvywqiqkeslfmlklhfskdggmn
kvqqckillrvwlkvvnwns*mvgiv*mmrllptmtilltk*hwainsfgriselcqr*a
gt*ihlaivqhklvslv---

---vhhgiypwfnqpvvimyx*mllltfkilinhynsplslievvdvhr*emvnwi**ci
vvl*kmmvvvlvnq*msqlkslqlvn*fsmifqvmlnliivxlhxlyhihysqclqplnn
lqtigilnikvfthh*lqxhlyqmv*rfklyng*iikiipyfyvlkifiklmvkihkihk
lsl*ifqlsfqqlqsllqlk*i*lvfknyqiyq

Frame B:
glleshthikmvikklfilifclfliineingkknknk*y*kiktktiiniikcsyccsy
s**cwlvkns**illwikyvncicrcsiyfgyshylfigksrkkvylc*ncifpkmvg*t
kynnakyc*gfg*kwsigihkwwvlye**gyyll*ryy*pndigpsiplgefrsyakdrl
ahrsiwp*cntssylws---

---simeftrgstnqw*lctcecyclhsrs*siitihhch**kswmcivkrwsigyddas
syfer*wswcwstne*vnsnryn**idfp*yfklcsislsscctxfitsitpnvynhsti
fkrlefsisrclltidfsxtftkwfkdsnstmvr*sr*yhtfty*kylsn*wsrftrstn
yhfrsfnylfnnynhfcn*nefnwcskiikfik

Frame C:
aywkatht*kw**knyly*ffvcf*LSMKLMEKKTKINDIKKSKPKLSSTLLNVHIVAHT
HDDVGWLKTVDEYYYGSNMSIAFAGVQYTLDTAITCLLANPERKFIYVEIAFFQRWWDEQ
STTMQNIVKGLVESGQLEFINGGYCMNDEATTYYDDTIDQMTLGHQFLWENFGVMPKIGW
HIDPFGHSATQARIFG---

---PSWNLPVVQPTSGNYVXVNAIAYIQDLNQSLQFTIVTDRSRGCASLRDGQLDMMMHR
RTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRXAALSLSHPLLPMFTTTQQS
SNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQT
ITLDLSTIFSTITITSATEMNLTGVQKLSNLS

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE761 (VHE761Q) /CSM/VH/VHE7-C/VHE761Q.Seq.d/                       2083   0.0
SHF209 (SHF209Q) /CSM/SH/SHF2-A/SHF209Q.Seq.d/                       1213   0.0
SHK860 (SHK860Q) /CSM/SH/SHK8-C/SHK860Q.Seq.d/                       1207   0.0
SHF812 (SHF812Q) /CSM/SH/SHF8-A/SHF812Q.Seq.d/                       1207   0.0
AHM429 (AHM429Q) /CSM/AH/AHM4-B/AHM429Q.Seq.d/                       1207   0.0
VHP767 (VHP767Q) /CSM/VH/VHP7-C/VHP767Q.Seq.d/                       1205   0.0
VHB838 (VHB838Q) /CSM/VH/VHB8-B/VHB838Q.Seq.d/                       1205   0.0
CHN525 (CHN525Q) /CSM/CH/CHN5-B/CHN525Q.Seq.d/                       1205   0.0
AHI659 (AHI659Q) /CSM/AH/AHI6-C/AHI659Q.Seq.d/                       1205   0.0
VHQ167 (VHQ167Q) /CSM/VH/VHQ1-C/VHQ167Q.Seq.d/                       1203   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1178   0.0    1
CK149800|CK149800.1 CsmgEST00336 Culicoides sonorensis female serum-fed midgut cDNA Library Culicoides sonorensis cDNA, mRNA sequence.   117   1e-23  3
DN814237|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   1e-16  4
DN816506|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   2e-16  4
CX633815|CX633815.1 taj44a03.y3 Hydra EST UCI 5 Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence.    52   2e-16  4
DN816232| truncated lysosomal acid alpha-mannosidase [Homo sapiens], mRNA sequence.    52   2e-16  4
DN811862| lysosomal alpha-mannosidase [Cavia porcellus], mRNA sequence.    52   3e-16  4
DT613126|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   3e-16  4
DN815247| lysosomal alpha-mannosidase [Cavia porcellus], mRNA sequence.    52   3e-16  4
CB073433|CB073433.1 taa32h01.y1 Hydra EST -II Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence.    52   2e-15  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   342   2e-92
(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   342   2e-92
AK068415_1(AK068415|pid:none) Oryza sativa Japonica Group cDNA c...   201   4e-50
AY099704_1(AY099704|pid:none) Arabidopsis thaliana alpha-mannosi...   199   2e-49
AB005230_20(AB005230|pid:none) Arabidopsis thaliana genomic DNA,...   199   2e-49
AB026648_12(AB026648|pid:none) Arabidopsis thaliana genomic DNA,...   199   2e-49
(Q29451) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   193   1e-47
AK291572_1(AK291572|pid:none) Homo sapiens cDNA FLJ76867 complet...   191   4e-47
U05572_1(U05572|pid:none) Human lysosomal alpha-mannosidase (MAN...   191   4e-47
AB209921_1(AB209921|pid:none) Homo sapiens mRNA for mannosidase,...   191   4e-47

 psg: 0.41  gvh: 0.43  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: vesicles of secretory system
	  4.0 %: vacuolar
	  4.0 %: mitochondrial

>> prediction for VHE761 is nuc

>VHE761F.Seq
TGGCCTACTGGAAAGCCACACACACATAAAAATGGTAATAAAAAAATTATTTATATTAAT
TTTTTGTTTGTTTTTAATTATCAATGAAATTAATGGAAAAAAAAACAAAAATAAATGATA
TTAAAAAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCATATTGTTGCTCATA
CTCATGATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTATGGATCAAATATGT
CAATTGCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACCTGTTTATTGGCAA
ATCCAGAAAGAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGATGAAC
AAAGTACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCA
TAAATGGTGGGTATTGTATGAATGATGAGGCTACTACCTACTATGACGATACTATTGACC
AAATGACATTGGGCCATCAATTCCTTTGGGAGAATTTCGGAGTTATGCCAAAGATAGGCT
GGCACATAGATCCATTTGGCCATAGTGCAACACAAGCTCGTATCTTTGGTCANNNNNNNN
NN

>VHE761Z.Seq
NNNNNNNNNNGTCCATCATGGAATTTACCCGTGGTTCAACCAACCAGTGGTAATTATGTA
CNTGTGAATGCTATTGCTTACATTCAAGATCTTAATCAATCATTACAATTCACCATTGTC
ACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGCAT
CGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAA
ATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTAT
CGTCNTGCTGCACTNTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAACAA
TCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCNTCA
CCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATACC
ATACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAA
ACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAA
ATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAG

>VHE761P.Seq
TGGCCTACTGGAAAGCCACACACACATAAAAATGGTAATAAAAAAATTATTTATATTAAT
TTTTTGTTTGTTTTTAATTATCAATGAAATTAATGGAAAAAAAAACAAAAATAAATGATA
TTAAAAAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCATATTGTTGCTCATA
CTCATGATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTATGGATCAAATATGT
CAATTGCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACCTGTTTATTGGCAA
ATCCAGAAAGAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGATGAAC
AAAGTACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCA
TAAATGGTGGGTATTGTATGAATGATGAGGCTACTACCTACTATGACGATACTATTGACC
AAATGACATTGGGCCATCAATTCCTTTGGGAGAATTTCGGAGTTATGCCAAAGATAGGCT
GGCACATAGATCCATTTGGCCATAGTGCAACACAAGCTCGTATCTTTGGTCA--------
--GTCCATCATGGAATTTACCCGTGGTTCAACCAACCAGTGGTAATTATGTACNTGTGAA
TGCTATTGCTTACATTCAAGATCTTAATCAATCATTACAATTCACCATTGTCACTGATAG
AAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTAC
TTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTAC
AACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCNTGC
TGCACTNTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAA
CGATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCNTCACCTTTACC
AAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTT
ACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCAC
TTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTT
AACTGGTGTTCAAAAATTATCAAATTTATCAAG