>VHE757 (VHE757Q) /CSM/VH/VHE7-C/VHE757Q.Seq.d/
TTAAACTTTATTTTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCT
CTTTAAACTCTTATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAACTAAAAC
AAAAAAAAATGGTCAATTTTACAATAGATCAAATTCGTTCAATTATGGATCGTCGTGAAA
ATATCANAAATATGTCTGTTATTGCTCATGTTGATCATGGTAAAACTACTCTTACTGATT
CATTAATTCAAAGAGCTGGTATTATTGCAGATAAGGTATCTGGTAATGCAGGATATATGT
CATGTCGTCCAGATGAACAATTACGTGGTATTACCATAAAGTCATCATCTGTTTCACTTC
ACTTTGAAATGCCAAAAGATGAGAAATTACCAACTGGTTGTAGCTCACACGAATTCTTGA
TCAATCTTATTGATTCACCAGGTCATGTTGATTTTAGTTCTGAAGTAACAGCAGCACTTC
GTGTTACCGATGGTGCATTGGTAGTTATCGATTGTGTTGAGGGTGTTTGTGTACAAACTG
AAACTGTTTTACGTCAAGCAATGGCTGAACGTATTAAACCXXXXXXXXXXTTAACTGATG
ATATTGATAATGGTTTGGTGGCTTTTAATCAAGATGTTAAAAGTCGTGCCAATTATCTCT
ATGATAAACATTCTTGGGATAAGAATGATGCAGTTAACATTTGGTCATTTGGTCCAGAAT
ATAAGGGTCCAAATGTTTTAGTTAACAAAACCAAAGGGTGTTCAATATCTTCATGAAATT
AAAGACTCTATGGTTTCAGCATTCCAATGGATCTCAAATGAAGGTGTATTATGTGGTGAG
AAAATGAGATCGATTCGTTTCAACGTTTATGATGTAATGCTTCATGCCGATGCAATTCAT
AGAGGTAGTGGTCAATTTATTCCAACAACTCGTCGTGTAATGTATGCNTCCCAACTATCA
GCTCAACCAACACTTTTGGAACCAATGTATTTGGTTGATATTAGTACTCCTGAAAGTGGT
ATTAGTGGTATCTACTCTGTACTCAATCGTAGAAGAGGTGTTGTCATTGGTGAAGAACGT
CGTATTGGTACTCCATTATTCAATATCAAAGCTTATCTTCCTGTAATGGAATCATTTGGT
CTCACTGCTGATTTACGTTCAAGTACTGCTGGTCAAGCTTTCCCTCAATGTGTTTTTGAT
CATTGGAGTTCAATTGGTATCGTGGTCAAGATAAAAGATCAACTGAAGTAGCTTCATCAA
TTAGAAAGAGAAAAGGTTTAAATCCAAAATTACCA

klyfsptlakl*fafvqlfslnsyy*nsf*KLSFLKKLKQKKMVNFTIDQIRSIMDRREN
IXNMSVIAHVDHGKTTLTDSLIQRAGIIADKVSGNAGYMSCRPDEQLRGITIKSSSVSLH
FEMPKDEKLPTGCSSHEFLINLIDSPGHVDFSSEVTAALRVTDGALVVIDCVEGVCVQTE
TVLRQAMAERIK---

---LTDDIDNGLVAFNQDVKSRANYLYDKHSWDKNDAVNIWSFGPEYKGPNVLVNKTKGC
SISS*n*rlygfsipmdlk*rcimw*eneidsfqrl*cnascrcns*r*wsiysnnsscn
vcxptisstntfgtnvfg*y*ys*kwy*wyllctqs*krcchw*rtsywysiiqyqslss
cngiiwshc*ftfkycwssfpsmcf*slefnwyrgqdkrstevassirkrkglnpklp

Frame A:
lnfifplhwpnfnlplynysl*tliikihfknshf*kn*nkkkwsilq*ikfvqlwivvk
isxicllllmlimvkllllih*fkelvllqirylvmqdichvvqmnnyvvlp*shhlfhf
tlkcqkmrnyqlvvahtns*sillihqvmlilvlk*qqhfvlpmvhw*lsivlrvfvykl
klfyvkqwlnvln---

---LTDDIDNGLVAFNQDVKSRANYLYDKHSWDKNDAVNIWSFGPEYKGPNVLVNKTKGC
SISS*n*rlygfsipmdlk*rcimw*eneidsfqrl*cnascrcns*r*wsiysnnsscn
vcxptisstntfgtnvfg*y*ys*kwy*wyllctqs*krcchw*rtsywysiiqyqslss
cngiiwshc*ftfkycwssfpsmcf*slefnwyrgqdkrstevassirkrkglnpklp

Frame B:
*tlffpyigqtliclctiilfkllllkfilktliskktktkkngqfynrsnsfnygss*k
yxkyvcycsc*sw*nysy*finskswyycr*giw*criyvmssr*titwyyhkviicfts
l*nakr*eitnwl*ltrildqsy*ftrsc*f*f*snsstscyrwcigsyrlc*gclctn*
ncftssng*ty*t---

---*lmilimvwwllikmlkvvpiisminilgirmmqltfghlvqnirvqmf*ltkpkgv
qylheikdsmvsafqwisnegvlcgekmrsirfnvydvmlhadaihrgsgqfipttrrvm
yasqlsaqptllepmylvdistpesgisgiysvlnrrrgvvigeerrigtplfnikaylp
vmesfgltadlrsstagqafpqcvfdhwssigivvkikdqlk*lhqlerekv*iqny

Frame C:
klyfsptlakl*fafvqlfslnsyy*nsf*KLSFLKKLKQKKMVNFTIDQIRSIMDRREN
IXNMSVIAHVDHGKTTLTDSLIQRAGIIADKVSGNAGYMSCRPDEQLRGITIKSSSVSLH
FEMPKDEKLPTGCSSHEFLINLIDSPGHVDFSSEVTAALRVTDGALVVIDCVEGVCVQTE
TVLRQAMAERIK---

---n**y**wfggf*src*kscqlsl**tflg*e*cs*hlviwsri*gskcfs*qnqrvf
nifmklktlwfqhsngsqmkvyyvvrk*drfvstfmm*cfmpmqfievvvnlfqqlvv*c
mxpnyqlnqhfwnqciwlilvllkvvlvvstlysiveevlslvknvvlvlhysisklifl
*wnhlvslliyvqvllvklslnvfliigvqlvswsr*kin*ssfin*kekrfkskit

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE757 (VHE757Q) /CSM/VH/VHE7-C/VHE757Q.Seq.d/                       2240   0.0
VHJ795 (VHJ795Q) /CSM/VH/VHJ7-D/VHJ795Q.Seq.d/                       1390   0.0
AHQ218 (AHQ218Q) /CSM/AH/AHQ2-A/AHQ218Q.Seq.d/                       1390   0.0
SHF216 (SHF216Q) /CSM/SH/SHF2-A/SHF216Q.Seq.d/                       1378   0.0
AHM516 (AHM516Q) /CSM/AH/AHM5-A/AHM516Q.Seq.d/                       1376   0.0
AHL348 (AHL348Q) /CSM/AH/AHL3-B/AHL348Q.Seq.d/                       1376   0.0
AHE765 (AHE765Q) /CSM/AH/AHE7-C/AHE765Q.Seq.d/                       1376   0.0
AHD146 (AHD146Q) /CSM/AH/AHD1-B/AHD146Q.Seq.d/                       1376   0.0
AHC654 (AHC654Q) /CSM/AH/AHC6-C/AHC654Q.Seq.d/                       1376   0.0
AHB739 (AHB739Q) /CSM/AH/AHB7-B/AHB739Q.Seq.d/                       1376   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.   240   e-142  9
X15386|X15386.1 Dictyostelium discoideum H6 gene mRNA.            228   7e-60  2
AY310285|AY310285.1 Scutigera coleoptrata voucher Scol elongation factor 2 mRNA, partial cds.   103   1e-30  3
EC822839|EC822839.1 SME00001738 esmbsro2 Sawyeria marylandensis cDNA, mRNA sequence.   123   1e-29  2
EC824047|EC824047.1 SME00009166 esmbsro2 Sawyeria marylandensis cDNA, mRNA sequence.   123   1e-29  2
EC825843|EC825843.1 SME00002609 esmbsro2 Sawyeria marylandensis cDNA, mRNA sequence.   123   2e-29  2
EC825885|EC825885.1 SME00004608 esmbsro2 Sawyeria marylandensis cDNA, mRNA sequence.   123   2e-29  2
CN563875|CN563875.1 taf95g04.x1 Hydra EST -Kiel 1 Hydra magnipapillata cDNA 3' similar to SW:EF2_MESAU P05086 ELONGATION FACTOR 2 ;, mRNA sequence.    70   7e-27  4
DY447957| Unknown (protein for MGC:55904) [Danio rerio], mRNA sequence.    70   1e-26  4
DY447570| Unknown (protein for MGC:55904) [Danio rerio], mRNA sequence.    70   1e-26  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   206   8e-66
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   205   7e-65
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   204   2e-64
(P05197) RecName: Full=Elongation factor 2;          Short=EF-2;...   204   2e-64
AK159514_1(AK159514|pid:none) Mus musculus osteoclast-like cell ...   204   2e-64
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   204   2e-64
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   204   2e-64
AJ278147_1(AJ278147|pid:none) Rattus norvegicus partial Ef-2 gen...   204   2e-64
BC063919_1(BC063919|pid:none) Xenopus tropicalis eukaryotic tran...   205   2e-64
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   204   2e-64

 psg: 0.66  gvh: 0.43  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.34  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 20.0 %: nuclear
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal

>> prediction for VHE757 is cyt

>VHE757F.Seq
TTAAACTTTATTTTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCT
CTTTAAACTCTTATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAACTAAAAC
AAAAAAAAATGGTCAATTTTACAATAGATCAAATTCGTTCAATTATGGATCGTCGTGAAA
ATATCANAAATATGTCTGTTATTGCTCATGTTGATCATGGTAAAACTACTCTTACTGATT
CATTAATTCAAAGAGCTGGTATTATTGCAGATAAGGTATCTGGTAATGCAGGATATATGT
CATGTCGTCCAGATGAACAATTACGTGGTATTACCATAAAGTCATCATCTGTTTCACTTC
ACTTTGAAATGCCAAAAGATGAGAAATTACCAACTGGTTGTAGCTCACACGAATTCTTGA
TCAATCTTATTGATTCACCAGGTCATGTTGATTTTAGTTCTGAAGTAACAGCAGCACTTC
GTGTTACCGATGGTGCATTGGTAGTTATCGATTGTGTTGAGGGTGTTTGTGTACAAACTG
AAACTGTTTTACGTCAAGCAATGGCTGAACGTATTAAACCNNNNNNNNNN

>VHE757Z.Seq
NNNNNNNNNNTTAACTGATGATATTGATAATGGTTTGGTGGCTTTTAATCAAGATGTTAA
AAGTCGTGCCAATTATCTCTATGATAAACATTCTTGGGATAAGAATGATGCAGTTAACAT
TTGGTCATTTGGTCCAGAATATAAGGGTCCAAATGTTTTAGTTAACAAAACCAAAGGGTG
TTCAATATCTTCATGAAATTAAAGACTCTATGGTTTCAGCATTCCAATGGATCTCAAATG
AAGGTGTATTATGTGGTGAGAAAATGAGATCGATTCGTTTCAACGTTTATGATGTAATGC
TTCATGCCGATGCAATTCATAGAGGTAGTGGTCAATTTATTCCAACAACTCGTCGTGTAA
TGTATGCNTCCCAACTATCAGCTCAACCAACACTTTTGGAACCAATGTATTTGGTTGATA
TTAGTACTCCTGAAAGTGGTATTAGTGGTATCTACTCTGTACTCAATCGTAGAAGAGGTG
TTGTCATTGGTGAAGAACGTCGTATTGGTACTCCATTATTCAATATCAAAGCTTATCTTC
CTGTAATGGAATCATTTGGTCTCACTGCTGATTTACGTTCAAGTACTGCTGGTCAAGCTT
TCCCTCAATGTGTTTTTGATCATTGGAGTTCAATTGGTATCGTGGTCAAGATAAAAGATC
AACTGAAGTAGCTTCATCAATTAGAAAGAGAAAAGGTTTAAATCCAAAATTACCA

>VHE757P.Seq
TTAAACTTTATTTTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCT
CTTTAAACTCTTATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAACTAAAAC
AAAAAAAAATGGTCAATTTTACAATAGATCAAATTCGTTCAATTATGGATCGTCGTGAAA
ATATCANAAATATGTCTGTTATTGCTCATGTTGATCATGGTAAAACTACTCTTACTGATT
CATTAATTCAAAGAGCTGGTATTATTGCAGATAAGGTATCTGGTAATGCAGGATATATGT
CATGTCGTCCAGATGAACAATTACGTGGTATTACCATAAAGTCATCATCTGTTTCACTTC
ACTTTGAAATGCCAAAAGATGAGAAATTACCAACTGGTTGTAGCTCACACGAATTCTTGA
TCAATCTTATTGATTCACCAGGTCATGTTGATTTTAGTTCTGAAGTAACAGCAGCACTTC
GTGTTACCGATGGTGCATTGGTAGTTATCGATTGTGTTGAGGGTGTTTGTGTACAAACTG
AAACTGTTTTACGTCAAGCAATGGCTGAACGTATTAAACC----------TTAACTGATG
ATATTGATAATGGTTTGGTGGCTTTTAATCAAGATGTTAAAAGTCGTGCCAATTATCTCT
ATGATAAACATTCTTGGGATAAGAATGATGCAGTTAACATTTGGTCATTTGGTCCAGAAT
ATAAGGGTCCAAATGTTTTAGTTAACAAAACCAAAGGGTGTTCAATATCTTCATGAAATT
AAAGACTCTATGGTTTCAGCATTCCAATGGATCTCAAATGAAGGTGTATTATGTGGTGAG
AAAATGAGATCGATTCGTTTCAACGTTTATGATGTAATGCTTCATGCCGATGCAATTCAT
AGAGGTAGTGGTCAATTTATTCCAACAACTCGTCGTGTAATGTATGCNTCCCAACTATCA
GCTCAACCAACACTTTTGGAACCAATGTATTTGGTTGATATTAGTACTCCTGAAAGTGGT
ATTAGTGGTATCTACTCTGTACTCAATCGTAGAAGAGGTGTTGTCATTGGTGAAGAACGT
CGTATTGGTACTCCATTATTCAATATCAAAGCTTATCTTCCTGTAATGGAATCATTTGGT
CTCACTGCTGATTTACGTTCAAGTACTGCTGGTCAAGCTTTCCCTCAATGTGTTTTTGAT
CATTGGAGTTCAATTGGTATCGTGGTCAAGATAAAAGATCAACTGAAGTAGCTTCATCAA
TTAGAAAGAGAAAAGGTTTAAATCCAAAATTACCA