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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE734
Library VH
(Link to library)
Clone ID VHE734
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14496-1
Original site URL
Representative seq. ID VHE734P
(Link to Original site)
Representative DNA sequence 
>VHE734 (VHE734Q) /CSM/VH/VHE7-B/VHE734Q.Seq.d/
AGTATCACATATTAAAATGCCAACCAGATTCAGTAAGCATAGAAAATCAAGAGGTGACGT
TTGTGCCGGTTACGGTCGTGTTGGTAAACACAGAAAGCATCCAGGTGGTCGTGGTAATGC
TGGTGGTCTCACTCATCACAGAATTAACTTTGACAAATACCATCCAGGTTATTTCGGTAA
ATTAGGTATGAGACATTTCCATTTATTAAGAAACCAATACCATTGTCCAACCGTCTCATT
AGAAAAGATCTGGACTTTAGTCCCAGAATCCGTCAGAAAATCATTAGCTGCCAAGAATGA
CGGTACTGCTCCAGTTGTTGATGTCACCCAAAAAGGTTTCTTCAAAGTTTTAGGTCACGG
TATCCTCCCAACTCAACCAATCATCGTCAAAGCTCGTTATTTCTCTAAAGTCGCTGAAAA
GAAAATCAAAGCTGTCGGTGGTGCTTGTATCTTAGTTGCTTAAATATTTTATACGAATCA
ATAAATATAAATAAAAAAAGTAATTAAATTTAAAAAATATTNNNNAAAAAAXXXXXXXXX
XATCACTCATCACAGAATTNNATTTGACAAATACCATCCAGGTTATTTCGGTANNTTAGG
TATNAGACATTTCCATTTATTAAGAAACCAATACCATTGTCCAACCGTCTCATTAGNAAA
GATNTGGNCTTTAGTCCCAGAATCCGTCAGAAAATCATTAGCTGCCAAGAATGACGGTAC
TGCTCCAGTTGTTGATGTCACCCAAAAAGGTNTCTTCAAAGTTTTAGGTCACGGTATCNT
CCCAANTCAACCAATCATCGTCAAAGCTCGTTATTTCTCTA
sequence update 2002.10.25
Translated Amino Acid sequence 
vshikmptrfskhrksrgdvcagygrvgkhrkhpggrgnagglthhrinfdkyhpgyfgk
lgmrhfhllrnqyhcptvslekiwtlvpesvrkslaakndgtapvvdvtqkgffkvlghg
ilptqpiivkaryfskvaekkikavggacilva*ifytnq*i*IKKVIKFKKYXXK---

---ITHHRIXFDKYHPGYFGXLGXRHFHLLRNQYHCPTVSLXKXWXLVPESVRKSLAAKN
DGTAPVVDVTQKGXFKVLGHGIXPXQPIIVKARYFS
Translated Amino Acid sequence (All Frames) 
Frame A:
sity*nanqiq*a*kikr*rlcrlrscw*tqkasrwsw*cwwshssqn*l*qipsrlfr*
iryetfpfikkpiplsnrlirkdldfsprirqkiiscqe*rycssc*chpkrflqsfrsr
yppnstnhrqsslfl*sr*kenqscrwclylsclnilyesininkksn*i*kixxkk---

---ITHHRIXFDKYHPGYFGXLGXRHFHLLRNQYHCPTVSLXKXWXLVPESVRKSLAAKN
DGTAPVVDVTQKGXFKVLGHGIXPXQPIIVKARYFS

Frame B:
vshikmptrfskhrksrgdvcagygrvgkhrkhpggrgnagglthhrinfdkyhpgyfgk
lgmrhfhllrnqyhcptvslekiwtlvpesvrkslaakndgtapvvdvtqkgffkvlghg
ilptqpiivkaryfskvaekkikavggacilva*ifytnq*i*IKKVIKFKKYXXK---

---slitexxltntiqvisvx*vxdisiy*etntivqpsh*xrxgl*sqnpsenh*lprm
tvllqllmspkkvsskf*vtvssqxnqsssklvisl

Frame C:
yhilkcqpdsvsienqevtfvpvtvvlvntesiqvvvvmlvvsliteltltntiqvisvn
*v*disiy*etntivqpsh*krsgl*sqnpsenh*lprmtvllqllmspkkvsskf*vtv
ssqlnqsssklvislkslkrksklsvvlvs*llkyfirinkyk*kk*lnlknixxk---

---hssqnxi*qipsrlfrxxryxtfpfikkpiplsnrlixkdxxfsprirqkiiscqe*
rycssc*chpkrxlqsfrsryxpxstnhrqsslfl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ737 (VSJ737Q) /CSM/VS/VSJ7-B/VSJ737Q.Seq.d/                        952   0.0
VHO146 (VHO146Q) /CSM/VH/VHO1-B/VHO146Q.Seq.d/                        952   0.0
VHG250 (VHG250Q) /CSM/VH/VHG2-C/VHG250Q.Seq.d/                        952   0.0
VHE734 (VHE734Q) /CSM/VH/VHE7-B/VHE734Q.Seq.d/                        952   0.0
VFF473 (VFF473Q) /CSM/VF/VFF4-D/VFF473Q.Seq.d/                        952   0.0
VFE287 (VFE287Q) /CSM/VF/VFE2-D/VFE287Q.Seq.d/                        952   0.0
VSI571 (VSI571Q) /CSM/VS/VSI5-C/VSI571Q.Seq.d/                        946   0.0
VSE523 (VSE523Q) /CSM/VS/VSE5-A/VSE523Q.Seq.d/                        946   0.0
VSA537 (VSA537Q) /CSM/VS/VSA5-B/VSA537Q.Seq.d/                        946   0.0
VSH826 (VSH826Q) /CSM/VS/VSH8-B/VSH826Q.Seq.d/                        942   0.0
own update 2002.12.26
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

D50338|D50338.1 Dictyostelium discoideum DNA for ribosomal protein, complete cds.   914   0.0    2
EC761146|EC761146.1 PSE00006029 rw_mgpallid Polysphondylium pallidum cDNA, mRNA sequence.   192   e-100  3
EC761779|EC761779.1 PSE00006738 rw_mgpallid Polysphondylium pallidum cDNA, mRNA sequence.   178   3e-48  2
EC762385|EC762385.1 PSE00005843 rw_mgpallid Polysphondylium pallidum cDNA, mRNA sequence.   178   3e-48  2
EC762011|EC762011.1 PSE00003335 rw_mgpallid Polysphondylium pallidum cDNA, mRNA sequence.   178   3e-48  2
EC762442|EC762442.1 PSE00007743 rw_mgpallid Polysphondylium pallidum cDNA, mRNA sequence.   153   1e-32  1
BW638999|BW638999.1 Dugesia ryukyuensis mRNA, clone: Dr_sW_013_F17, 5' mRNA sequence.    84   4e-28  2
CK988951|CK988951.1 BgHC-16.54 BgHC Biomphalaria glabrata cDNA 5', mRNA sequence.    84   6e-27  2
DT724516|DT724516.1 BGE00625 Biomphalaria glabrata (Bge)cell line Library in Lambda ZAP Biomphalaria glabrata cDNA clone RBGKJ11TR, mRNA sequence.    84   6e-27  2
DT724867|DT724867.1 BGE00979 Biomphalaria glabrata (Bge)cell line Library in Lambda ZAP Biomphalaria glabrata cDNA clone RBGKN72TR, mRNA sequence.    84   6e-27  2
dna update 2007. 9. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P48160) RecName: Full=60S ribosomal protein L27a;  &D50338_1(D5...   313   6e-84
BT046766_1(BT046766|pid:none) Salmo salar clone ssal-eve-548-138...   226   5e-58
AY961477_1(AY961477|pid:none) Phytophthora infestans clone MY-19...   226   9e-58
AF502247_1(AF502247|pid:none) Epinephelus coioides ribosomal pro...   223   8e-57
AJ843085_1(AJ843085|pid:none) Platichthys flesus mRNA for 60S ri...   223   8e-57
(P18445) RecName: Full=60S ribosomal protein L27a;  &R5RTLA(S130...   222   1e-56
AB374966_1(AB374966|pid:none) Solea senegalensis RPL27a mRNA for...   221   2e-56
(P46776) RecName: Full=60S ribosomal protein L27a;  &(Q5REY2) Re...   221   2e-56
AJ307670_3(AJ307670|pid:none) Mus musculus St5 gene, L27a gene a...   221   2e-56
AB172987_1(AB172987|pid:none) Macaca fascicularis brain cDNA clo...   220   4e-56
protein update 2009. 7. 1
PSORT 

 psg: 0.64  gvh: 0.36  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.37  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: mitochondrial
	 20.0 %: cytoplasmic
	 16.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VHE734 is mit
5' end seq. ID VHE734F
5' end seq. 
>VHE734F.Seq
AGTATCACATATTAAAATGCCAACCAGATTCAGTAAGCATAGAAAATCAAGAGGTGACGT
TTGTGCCGGTTACGGTCGTGTTGGTAAACACAGAAAGCATCCAGGTGGTCGTGGTAATGC
TGGTGGTCTCACTCATCACAGAATTAACTTTGACAAATACCATCCAGGTTATTTCGGTAA
ATTAGGTATGAGACATTTCCATTTATTAAGAAACCAATACCATTGTCCAACCGTCTCATT
AGAAAAGATCTGGACTTTAGTCCCAGAATCCGTCAGAAAATCATTAGCTGCCAAGAATGA
CGGTACTGCTCCAGTTGTTGATGTCACCCAAAAAGGTTTCTTCAAAGTTTTAGGTCACGG
TATCCTCCCAACTCAACCAATCATCGTCAAAGCTCGTTATTTCTCTAAAGTCGCTGAAAA
GAAAATCAAAGCTGTCGGTGGTGCTTGTATCTTAGTTGCTTAAATATTTTATACGAATCA
ATAAATATAAATAAAAAAAGTAATTAAATTTAAAAAATATTNNNNAAAAAANNNNNNNNN
N
Length of 5' end seq. 541
3' end seq. ID VHE734Z
3' end seq. 
>VHE734Z.Seq
NNNNNNNNNNATCACTCATCACAGAATTNNATTTGACAAATACCATCCAGGTTATTTCGG
TANNTTAGGTATNAGACATTTCCATTTATTAAGAAACCAATACCATTGTCCAACCGTCTC
ATTAGNAAAGATNTGGNCTTTAGTCCCAGAATCCGTCAGAAAATCATTAGCTGCCAAGAA
TGACGGTACTGCTCCAGTTGTTGATGTCACCCAAAAAGGTNTCTTCAAAGTTTTAGGTCA
CGGTATCNTCCCAANTCAACCAATCATCGTCAAAGCTCGTTATTTCTCTA
Length of 3' end seq. 290
Connected seq. ID VHE734P
Connected seq. 
>VHE734P.Seq
AGTATCACATATTAAAATGCCAACCAGATTCAGTAAGCATAGAAAATCAAGAGGTGACGT
TTGTGCCGGTTACGGTCGTGTTGGTAAACACAGAAAGCATCCAGGTGGTCGTGGTAATGC
TGGTGGTCTCACTCATCACAGAATTAACTTTGACAAATACCATCCAGGTTATTTCGGTAA
ATTAGGTATGAGACATTTCCATTTATTAAGAAACCAATACCATTGTCCAACCGTCTCATT
AGAAAAGATCTGGACTTTAGTCCCAGAATCCGTCAGAAAATCATTAGCTGCCAAGAATGA
CGGTACTGCTCCAGTTGTTGATGTCACCCAAAAAGGTTTCTTCAAAGTTTTAGGTCACGG
TATCCTCCCAACTCAACCAATCATCGTCAAAGCTCGTTATTTCTCTAAAGTCGCTGAAAA
GAAAATCAAAGCTGTCGGTGGTGCTTGTATCTTAGTTGCTTAAATATTTTATACGAATCA
ATAAATATAAATAAAAAAAGTAATTAAATTTAAAAAATATTNNNNAAAAAA---------
-ATCACTCATCACAGAATTNNATTTGACAAATACCATCCAGGTTATTTCGGTANNTTAGG
TATNAGACATTTCCATTTATTAAGAAACCAATACCATTGTCCAACCGTCTCATTAGNAAA
GATNTGGNCTTTAGTCCCAGAATCCGTCAGAAAATCATTAGCTGCCAAGAATGACGGTAC
TGCTCCAGTTGTTGATGTCACCCAAAAAGGTNTCTTCAAAGTTTTAGGTCACGGTATCNT
CCCAANTCAACCAATCATCGTCAAAGCTCGTTATTTCTCTA
Length of connected seq. 811
Full length Seq ID -
Full length Seq. -
Length of full length seq. -