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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE733
Library VH
(Link to library)
Clone ID VHE733
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10794-1
Original site URL
Representative seq. ID VHE733P
(Link to Original site)
Representative DNA sequence 
>VHE733 (VHE733Q) /CSM/VH/VHE7-B/VHE733Q.Seq.d/
ATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTTATT
TTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACTCTT
ATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAACTAAAACAAAAAAAAAANG
GTCAATTTTACAATAGATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCAAAAAT
ATGTCTGTTATTGCTCATGTTGATCATGGTAAAACTACTCTTACTGATTCATTAATTCAA
AGAGCTGGTATTATTGCANATAAGGTATCTGGTAATGCAGGATATATGTCATGTCGTCCA
NATGAACAATTACGTGGTATTACCATAAAGTCATCATCTGTTTCACTTCACTTTGAAANG
CCAAAAGAXXXXXXXXXXATCGTCAGACTTAACTGATGATATTGATAATGGTTTGGTGGC
TTTTAATCAAGATGTTAAAAGTCGTGCCAATTATCTCTATGATAAACATTCTTGGGATAA
GAATGATGCAGTTAACATTTGGTCATTTGGTCCAGAATATAAGGGTCCAAATGTTTTAGT
TAACAAAACCAAAGGGTGTTCAATATCTTCATGAAATTAAAGACTCTATGGTTTCAGCAT
TCCAATGGATCTCAAATGAAGGTGTATTATGTGGTGAGAAAATGAGATCGATTCGTTTCA
ACGTTTATGATGTAATGCTTCATGCCGATGCAATTCATAGAGGTAGTGGTCAATTTATTC
CAACAACTCGTCGTGTAATGTATGCNTCCCAACTATCAGCTCAACCAACACTTTTGGAAC
CAATGTATTTGGTTGATATTAGTACTCCTGAAAGTGGTATTAGTGGTATCTACTCTGTAC
TCAATCGTAGAAGAGGTGTTGTCATTGGTGAAGAACGTCGTATTGGTACTCCATTATTCA
ATATCAAAGCTTATCTTCCTGTAATGGAATCATTTGGTCTCACTGCTGATTTACGTTCAA
GTACTGCTGGTCAAGCTTTCCCTCAATGTGTTTTTGATCATTGGAGTTCAATTGGTATCG
TTGGTCAAGAAAAAAAGAAT
sequence update 2002.10.25
Translated Amino Acid sequence 
isiiniitytkqn**nlnfifplhwpnfnlplynysl*tliikihfknshf*kn*NKKKX
VNFTIDQIRSIMDRRENIKNMSVIAHVDHGKTTLTDSLIQRAGIIAXKVSGNAGYMSCRP
XEQLRGITIKSSSVSLHFEXPK---

---SSDLTDDIDNGLVAFNQDVKSRANYLYDKHSWDKNDAVNIWSFGPEYKGPNVLVNKT
KGCSISS*n*rlygfsipmdlk*rcimw*eneidsfqrl*cnascrcns*r*wsiysnns
scnvcxptisstntfgtnvfg*y*ys*kwy*wyllctqs*krcchw*rtsywysiiqyqs
lsscngiiwshc*ftfkycwssfpsmcf*slefnwyrwsrkke
Translated Amino Acid sequence (All Frames) 
Frame A:
isiiniitytkqn**nlnfifplhwpnfnlplynysl*tliikihfknshf*kn*NKKKX
VNFTIDQIRSIMDRRENIKNMSVIAHVDHGKTTLTDSLIQRAGIIAXKVSGNAGYMSCRP
XEQLRGITIKSSSVSLHFEXPK---

---ivrln**y**wfggf*src*kscqlsl**tflg*e*cs*hlviwsri*gskcfs*qn
qrvfnifmklktlwfqhsngsqmkvyyvvrk*drfvstfmm*cfmpmqfievvvnlfqql
vv*cmxpnyqlnqhfwnqciwlilvllkvvlvvstlysiveevlslvknvvlvlhysisk
lifl*wnhlvslliyvqvllvklslnvfliigvqlvslvkkkr

Frame B:
yq**illhiqnkinkt*tlffpyigqtliclctiilfkllllkfilktliskktktkkkx
silq*ikfvqlwivxkiskicllllmlimvkllllih*fkelvllxirylvmqdichvvx
mnnyvvlp*shhlfhftlkxqk---

---SSDLTDDIDNGLVAFNQDVKSRANYLYDKHSWDKNDAVNIWSFGPEYKGPNVLVNKT
KGCSISS*n*rlygfsipmdlk*rcimw*eneidsfqrl*cnascrcns*r*wsiysnns
scnvcxptisstntfgtnvfg*y*ys*kwy*wyllctqs*krcchw*rtsywysiiqyqs
lsscngiiwshc*ftfkycwssfpsmcf*slefnwyrwsrkke

Frame C:
innkyyyiyktkliklklyfsptlakl*fafvqlfslnsyy*nsf*klsflkklkqkkxg
qfynrsnsfnygssxkyqkyvcycsc*sw*nysy*finskswyycx*giw*criyvmssx
*titwyyhkviicftsl*xakr---

---rqt*lmilimvwwllikmlkvvpiisminilgirmmqltfghlvqnirvqmf*ltkp
kgvqylheikdsmvsafqwisnegvlcgekmrsirfnvydvmlhadaihrgsgqfipttr
rvmyasqlsaqptllepmylvdistpesgisgiysvlnrrrgvvigeerrigtplfnika
ylpvmesfgltadlrsstagqafpqcvfdhwssigivgqekkn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE733 (VHE733Q) /CSM/VH/VHE7-B/VHE733Q.Seq.d/                       1715   0.0
SHF216 (SHF216Q) /CSM/SH/SHF2-A/SHF216Q.Seq.d/                       1285   0.0
VHB364 (VHB364Q) /CSM/VH/VHB3-C/VHB364Q.Seq.d/                       1277   0.0
SHA256 (SHA256Q) /CSM/SH/SHA2-C/SHA256Q.Seq.d/                       1277   0.0
AHM516 (AHM516Q) /CSM/AH/AHM5-A/AHM516Q.Seq.d/                       1277   0.0
AHL348 (AHL348Q) /CSM/AH/AHL3-B/AHL348Q.Seq.d/                       1277   0.0
AHE765 (AHE765Q) /CSM/AH/AHE7-C/AHE765Q.Seq.d/                       1277   0.0
AHD146 (AHD146Q) /CSM/AH/AHD1-B/AHD146Q.Seq.d/                       1277   0.0
AHB739 (AHB739Q) /CSM/AH/AHB7-B/AHB739Q.Seq.d/                       1277   0.0
AHB695 (AHB695Q) /CSM/AH/AHB6-D/AHB695Q.Seq.d/                       1277   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.   143   9e-84  8
X15386|X15386.1 Dictyostelium discoideum H6 gene mRNA.            228   6e-60  2
EV853155|EV853155.1 D.viviparus_35_F07.ab1 Dictyocaulus viviparus HannoverDv2000 cDNA Dictyocaulus viviparus cDNA, mRNA sequence.    82   2e-18  2
EV852655|EV852655.1 D.viviparus_32_D07.ab1 Dictyocaulus viviparus HannoverDv2000 cDNA Dictyocaulus viviparus cDNA, mRNA sequence.    82   2e-18  2
BI863585|BI863585.1 kx46a06.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CAEEL P29691 ELONGATION FACTOR 2 ;, mRNA sequence.    66   6e-18  4
EV854166|EV854166.1 D.viviparus_54_E01.ab1 Dictyocaulus viviparus HannoverDv2000 cDNA Dictyocaulus viviparus cDNA, mRNA sequence.    76   9e-17  2
EV854134|EV854134.1 D.viviparus_54_A03.ab1 Dictyocaulus viviparus HannoverDv2000 cDNA Dictyocaulus viviparus cDNA, mRNA sequence.    76   9e-17  2
EV853501|EV853501.1 D.viviparus_43_F03.ab1 Dictyocaulus viviparus HannoverDv2000 cDNA Dictyocaulus viviparus cDNA, mRNA sequence.    76   1e-16  2
BI863575|BI863575.1 kx45h07.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CHICK Q90705 ELONGATION FACTOR 2 ;, mRNA sequence.    66   1e-14  3
BI451383|BI451383.1 kx23e09.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CAEEL P29691 ELONGATION FACTOR 2 ;, mRNA sequence.    66   1e-14  3
dna update 2007. 9. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   206   2e-65
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   205   1e-64
BC063919_1(BC063919|pid:none) Xenopus tropicalis eukaryotic tran...   205   2e-64
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   204   2e-64
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   204   5e-64
(P05197) RecName: Full=Elongation factor 2;          Short=EF-2;...   204   5e-64
AK159514_1(AK159514|pid:none) Mus musculus osteoclast-like cell ...   204   5e-64
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   204   5e-64
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   204   5e-64
AJ278147_1(AJ278147|pid:none) Rattus norvegicus partial Ef-2 gen...   204   5e-64
protein update 2009. 7. 1
PSORT 

 psg: 0.47  gvh: 0.43  alm: 0.50  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: vacuolar

>> prediction for VHE733 is cyt
5' end seq. ID VHE733F
5' end seq. 
>VHE733F.Seq
ATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTTATT
TTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACTCTT
ATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAACTAAAACAAAAAAAAAANG
GTCAATTTTACAATAGATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCAAAAAT
ATGTCTGTTATTGCTCATGTTGATCATGGTAAAACTACTCTTACTGATTCATTAATTCAA
AGAGCTGGTATTATTGCANATAAGGTATCTGGTAATGCAGGATATATGTCATGTCGTCCA
NATGAACAATTACGTGGTATTACCATAAAGTCATCATCTGTTTCACTTCACTTTGAAANG
CCAAAAGANNNNNNNNNN
Length of 5' end seq. 438
3' end seq. ID VHE733Z
3' end seq. 
>VHE733Z.Seq
NNNNNNNNNNATCGTCAGACTTAACTGATGATATTGATAATGGTTTGGTGGCTTTTAATC
AAGATGTTAAAAGTCGTGCCAATTATCTCTATGATAAACATTCTTGGGATAAGAATGATG
CAGTTAACATTTGGTCATTTGGTCCAGAATATAAGGGTCCAAATGTTTTAGTTAACAAAA
CCAAAGGGTGTTCAATATCTTCATGAAATTAAAGACTCTATGGTTTCAGCATTCCAATGG
ATCTCAAATGAAGGTGTATTATGTGGTGAGAAAATGAGATCGATTCGTTTCAACGTTTAT
GATGTAATGCTTCATGCCGATGCAATTCATAGAGGTAGTGGTCAATTTATTCCAACAACT
CGTCGTGTAATGTATGCNTCCCAACTATCAGCTCAACCAACACTTTTGGAACCAATGTAT
TTGGTTGATATTAGTACTCCTGAAAGTGGTATTAGTGGTATCTACTCTGTACTCAATCGT
AGAAGAGGTGTTGTCATTGGTGAAGAACGTCGTATTGGTACTCCATTATTCAATATCAAA
GCTTATCTTCCTGTAATGGAATCATTTGGTCTCACTGCTGATTTACGTTCAAGTACTGCT
GGTCAAGCTTTCCCTCAATGTGTTTTTGATCATTGGAGTTCAATTGGTATCGTTGGTCAA
GAAAAAAAGAAT
Length of 3' end seq. 672
Connected seq. ID VHE733P
Connected seq. 
>VHE733P.Seq
ATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTTATT
TTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACTCTT
ATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAACTAAAACAAAAAAAAAANG
GTCAATTTTACAATAGATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCAAAAAT
ATGTCTGTTATTGCTCATGTTGATCATGGTAAAACTACTCTTACTGATTCATTAATTCAA
AGAGCTGGTATTATTGCANATAAGGTATCTGGTAATGCAGGATATATGTCATGTCGTCCA
NATGAACAATTACGTGGTATTACCATAAAGTCATCATCTGTTTCACTTCACTTTGAAANG
CCAAAAGA----------ATCGTCAGACTTAACTGATGATATTGATAATGGTTTGGTGGC
TTTTAATCAAGATGTTAAAAGTCGTGCCAATTATCTCTATGATAAACATTCTTGGGATAA
GAATGATGCAGTTAACATTTGGTCATTTGGTCCAGAATATAAGGGTCCAAATGTTTTAGT
TAACAAAACCAAAGGGTGTTCAATATCTTCATGAAATTAAAGACTCTATGGTTTCAGCAT
TCCAATGGATCTCAAATGAAGGTGTATTATGTGGTGAGAAAATGAGATCGATTCGTTTCA
ACGTTTATGATGTAATGCTTCATGCCGATGCAATTCATAGAGGTAGTGGTCAATTTATTC
CAACAACTCGTCGTGTAATGTATGCNTCCCAACTATCAGCTCAACCAACACTTTTGGAAC
CAATGTATTTGGTTGATATTAGTACTCCTGAAAGTGGTATTAGTGGTATCTACTCTGTAC
TCAATCGTAGAAGAGGTGTTGTCATTGGTGAAGAACGTCGTATTGGTACTCCATTATTCA
ATATCAAAGCTTATCTTCCTGTAATGGAATCATTTGGTCTCACTGCTGATTTACGTTCAA
GTACTGCTGGTCAAGCTTTCCCTCAATGTGTTTTTGATCATTGGAGTTCAATTGGTATCG
TTGGTCAAGAAAAAAAGAAT
Length of connected seq. 1090
Full length Seq ID -
Full length Seq. -
Length of full length seq. -