>VHE702 (VHE702Q) /CSM/VH/VHE7-A/VHE702Q.Seq.d/
ATTTCAATGAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATGAAAACTA
TTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCAACATATG
GAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAAAAACCAC
GTACTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATTTATCCAAAGAAAC
AATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTTGTTGATAATTTAA
TGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATTACAACAT
TAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCTAAAACTT
ATTATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCAGGTGATATTCATC
ATATTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACCATTGTTATCGAAG
GTAGTCCAAATAAGATTCCAATGATTTATGGTTTAGATTCAGTACATGGTGCAAAXXXXX
XXXXXACCAGTAGTTTTAATTTTGGTTGAAGCTCGTCCAAGAATTTTACCACCAGATCTC
GTCTACAGTTGTGCTGCAGTTTTAATGGCTTATNTCCCAGGTAGTGAAGGTGGTAAACCA
ATCGCCAACATTATCATGGGTAATGTAAATCCATCTGGTCGTCTTCCTTTAACTTATCCA
GGTACTACTGGTGATATCGGTGTTCCATATTACCACAAATACAGTGAAAATGGTGTCACC
ACTCCAATCTTCCAATTTGGTGATGGTCTTTCTTATACCACTTTTAACTATACCAATCTC
GCCTGTAGCAATTGTAAACCAATNTCTGGTCAATCTGGTAACTACACTGGTGTTTTAGGT
CAATCCTATACTTTCACTGTCACTGTCACAAACAATGGTAACGTTCAAGGTAAAGACTCT
GTTNTCTTATATCTCTCTGATCTTTGGGCTCAAGTTACTCCAGAAGTTAAAATGTTAAGA
GGTTTCCAAAAAGTTGATCTTAGTGCCAGCTAAATCTCAACAAATCTCTTTTACCCTCAA
GGCCTATGAATTCTCCTTTATTGGTGTCGATAATAAAATTACTTTAGAATCGGTCAATTC
ATCATTAGGTGGAATCAACAATTAGG

FNEYLIKFNFIYIYKKMKTIKSLFLLSLLIVNLLISSTYGSSIRVSIVGGEEAEVIEKPR
TFGNKRELKLEYSQIYPKKQLNQENINFMSARDTFVDNLMSKMSITEKIGQMTQLDITTL
TSPNTITINETTLAYYAKTYYIGSYLNSPVSGGLAGDIHHINSSVWLDMINTIQTIVIEG
SPNKIPMIYGLDSVHGA---

---PVVLILVEARPRILPPDLVYSCAAVLMAYXPGSEGGKPIANIIMGNVNPSGRLPLTY
PGTTGDIGVPYYHKYSENGVTTPIFQFGDGLSYTTFNYTNLACSNCKPXSGQSGNYTGVL
GQSYTFTVTVTNNGNVQGKDSVXLYLSDLWAQVTPEVKMLRGFQKVDLSAS*istnlfyp
qgl*illywcr**nyfrigqfiirwnqql

Frame A:
ismni*lnsilyiyikr*kllkvyfychc***iy**vqhmevqle*allevkklkslknh
vlleikensn*sivkfiqrnn*ikkilisclpeilllii*cqkcqslkklvk*lnwilqh
*hhqiqlqlmkqh*himlkliildhi*ihqyqvv*qvifiilivvfg*i*lilfkpllsk
vvqirfq*fmv*iqymvq---

---tssfnfg*sssknfttrsrlqlccsfnglxpr**rw*tnrqhyhg*cksiwsssfnl
sryyw*yrcsilpqiq*kwchhsnlpiw*wsflyhf*lyqsrl*ql*tnxwsiw*lhwcf
rsilyfhchchkqw*rsr*rlcxlisl*slgssysrs*nvkrfpks*s*cqlnlnksllp
srpmnspllvsiikll*nrsihh*vestir

Frame B:
fq*ifn*iqfyiyi*kdeny*kfiftvivdsefinkfniwkfn*skhcwr*rs*sh*ktt
yfwk*krtqirv*snlsketiksrky*fhvcqryfc**fnvknvnh*knwsndsigyyni
nitkynyn**nnisilc*nllywiifkftsirwfsr*yssy***clvryd*yysnhcyrr
*sk*dsndlwfrfstwck---

---PVVLILVEARPRILPPDLVYSCAAVLMAYXPGSEGGKPIANIIMGNVNPSGRLPLTY
PGTTGDIGVPYYHKYSENGVTTPIFQFGDGLSYTTFNYTNLACSNCKPXSGQSGNYTGVL
GQSYTFTVTVTNNGNVQGKDSVXLYLSDLWAQVTPEVKMLRGFQKVDLSAS*istnlfyp
qgl*illywcr**nyfrigqfiirwnqql

Frame C:
FNEYLIKFNFIYIYKKMKTIKSLFLLSLLIVNLLISSTYGSSIRVSIVGGEEAEVIEKPR
TFGNKRELKLEYSQIYPKKQLNQENINFMSARDTFVDNLMSKMSITEKIGQMTQLDITTL
TSPNTITINETTLAYYAKTYYIGSYLNSPVSGGLAGDIHHINSSVWLDMINTIQTIVIEG
SPNKIPMIYGLDSVHGA---

---q*f*fwlklvqefyhqisstvvlqf*wlxsqvvkvvnqsptlswvm*ihlvvfl*li
qvllvisvfhittntvkmvsplqssnlvmvfliplltipispvaivnqxlvnlvttlvf*
vnpilslslsqtmvtfkvktlxsyislifglkllqklkc*evskklilvpaksqqisftl
kayefsfigvdnkitlesvnsslgginn*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE702 (VHE702Q) /CSM/VH/VHE7-A/VHE702Q.Seq.d/                       2264   0.0
VHN466 (VHN466Q) /CSM/VH/VHN4-C/VHN466Q.Seq.d/                       1174   0.0
VHJ810 (VHJ810Q) /CSM/VH/VHJ8-A/VHJ810Q.Seq.d/                       1174   0.0
VHD249 (VHD249Q) /CSM/VH/VHD2-C/VHD249Q.Seq.d/                       1174   0.0
VHA656 (VHA656Q) /CSM/VH/VHA6-C/VHA656Q.Seq.d/                       1174   0.0
VHA640 (VHA640Q) /CSM/VH/VHA6-B/VHA640Q.Seq.d/                       1174   0.0
VHA467 (VHA467Q) /CSM/VH/VHA4-C/VHA467Q.Seq.d/                       1174   0.0
VHQ129 (VHQ129Q) /CSM/VH/VHQ1-B/VHQ129Q.Seq.d/                       1172   0.0
VHP578 (VHP578Q) /CSM/VH/VHP5-D/VHP578Q.Seq.d/                       1172   0.0
CHL451 (CHL451Q) /CSM/CH/CHL4-C/CHL451Q.Seq.d/                       1172   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L21014|L21014.1 Dictyostelium discoideum beta-glucosidase mRNA, complete cds.  1090   0.0    2
EH013950|EH013950.1 USDA-FP_186699 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt064_C10 5', mRNA sequence.    40   0.036  2
BX293980|BX293980.2 Mycoplasma mycoides subsp. mycoides SC str. PG1, complete genome.    32   0.050  15
BG139849|BG139849.1 EST480291 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP14B10 5' sequence, mRNA sequence.    48   0.12   2
CN215175|CN215175.1 28949 Suspension culture Solanum tuberosum cDNA, mRNA sequence.    40   0.27   3
AC117081|AC117081.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.    38   0.55   7
AC163188|AC163188.2 Bos taurus clone CH240-125O20, *** SEQUENCING IN PROGRESS ***, 10 unordered pieces.    44   0.58   5
BQ969550|BQ969550.1 QHB38N02.yg.ab1 QH_ABCDI sunflower RHA801 Helianthus annuus cDNA clone QHB38N02, mRNA sequence.    48   0.64   1
AC073286|AC073286.5 Homo sapiens chromosome 3 clone RP11-298M2, *** SEQUENCING IN PROGRESS ***, 47 unordered pieces.    48   0.64   1
AC067915|AC067915.2 Homo sapiens clone RP11-131G15, WORKING DRAFT SEQUENCE, 21 unordered pieces.    48   0.64   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A49881(A49881)beta-glucosidase (EC 3.2.1.21) precursor, lysosoma...   319   4e-93
CP000159_2777(CP000159|pid:none) Salinibacter ruber DSM 13855, c...   111   8e-26
CP000083_2301(CP000083|pid:none) Colwellia psychrerythraea 34H, ...   110   1e-22
CP000383_2239(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   102   4e-22
CP000383_3519(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   107   9e-22
CP000388_3699(CP000388|pid:none) Pseudoalteromonas atlantica T6c...   104   6e-21
CP000383_2243(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   101   5e-20
CP001146_680(CP001146|pid:none) Dictyoglomus thermophilum H-6-12...    97   9e-20
CP000685_3497(CP000685|pid:none) Flavobacterium johnsoniae UW101...   100   1e-19
AE017262_2747(AE017262|pid:none) Listeria monocytogenes str. 4b ...   100   1e-19

 psg: 0.74  gvh: 0.73  alm: 0.26  top: 0.07  tms: 0.07  mit: 0.34  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.79  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	 20.0 %: endoplasmic reticulum
	 16.0 %: Golgi
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VHE702 is cyt

>VHE702F.Seq
ATTTCAATGAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATGAAAACTA
TTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCAACATATG
GAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAAAAACCAC
GTACTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATTTATCCAAAGAAAC
AATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTTGTTGATAATTTAA
TGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATTACAACAT
TAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCTAAAACTT
ATTATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCAGGTGATATTCATC
ATATTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACCATTGTTATCGAAG
GTAGTCCAAATAAGATTCCAATGATTTATGGTTTAGATTCAGTACATGGTGCAAANNNNN
NNNNN

>VHE702Z.Seq
NNNNNNNNNNACCAGTAGTTTTAATTTTGGTTGAAGCTCGTCCAAGAATTTTACCACCAG
ATCTCGTCTACAGTTGTGCTGCAGTTTTAATGGCTTATNTCCCAGGTAGTGAAGGTGGTA
AACCAATCGCCAACATTATCATGGGTAATGTAAATCCATCTGGTCGTCTTCCTTTAACTT
ATCCAGGTACTACTGGTGATATCGGTGTTCCATATTACCACAAATACAGTGAAAATGGTG
TCACCACTCCAATCTTCCAATTTGGTGATGGTCTTTCTTATACCACTTTTAACTATACCA
ATCTCGCCTGTAGCAATTGTAAACCAATNTCTGGTCAATCTGGTAACTACACTGGTGTTT
TAGGTCAATCCTATACTTTCACTGTCACTGTCACAAACAATGGTAACGTTCAAGGTAAAG
ACTCTGTTNTCTTATATCTCTCTGATCTTTGGGCTCAAGTTACTCCAGAAGTTAAAATGT
TAAGAGGTTTCCAAAAAGTTGATCTTAGTGCCAGCTAAATCTCAACAAATCTCTTTTACC
CTCAAGGCCTATGAATTCTCCTTTATTGGTGTCGATAATAAAATTACTTTAGAATCGGTC
AATTCATCATTAGGTGGAATCAACAATTAGG

>VHE702P.Seq
ATTTCAATGAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATGAAAACTA
TTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCAACATATG
GAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAAAAACCAC
GTACTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATTTATCCAAAGAAAC
AATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTTGTTGATAATTTAA
TGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATTACAACAT
TAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCTAAAACTT
ATTATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCAGGTGATATTCATC
ATATTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACCATTGTTATCGAAG
GTAGTCCAAATAAGATTCCAATGATTTATGGTTTAGATTCAGTACATGGTGCAAA-----
-----ACCAGTAGTTTTAATTTTGGTTGAAGCTCGTCCAAGAATTTTACCACCAGATCTC
GTCTACAGTTGTGCTGCAGTTTTAATGGCTTATNTCCCAGGTAGTGAAGGTGGTAAACCA
ATCGCCAACATTATCATGGGTAATGTAAATCCATCTGGTCGTCTTCCTTTAACTTATCCA
GGTACTACTGGTGATATCGGTGTTCCATATTACCACAAATACAGTGAAAATGGTGTCACC
ACTCCAATCTTCCAATTTGGTGATGGTCTTTCTTATACCACTTTTAACTATACCAATCTC
GCCTGTAGCAATTGTAAACCAATNTCTGGTCAATCTGGTAACTACACTGGTGTTTTAGGT
CAATCCTATACTTTCACTGTCACTGTCACAAACAATGGTAACGTTCAAGGTAAAGACTCT
GTTNTCTTATATCTCTCTGATCTTTGGGCTCAAGTTACTCCAGAAGTTAAAATGTTAAGA
GGTTTCCAAAAAGTTGATCTTAGTGCCAGCTAAATCTCAACAAATCTCTTTTACCCTCAA
GGCCTATGAATTCTCCTTTATTGGTGTCGATAATAAAATTACTTTAGAATCGGTCAATTC
ATCATTAGGTGGAATCAACAATTAGG