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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE632
Library VH
(Link to library)
Clone ID VHE632
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U12301-1
Original site URL
Representative seq. ID VHE632P
(Link to Original site)
Representative DNA sequence 
>VHE632 (VHE632Q) /CSM/VH/VHE6-B/VHE632Q.Seq.d/
AATATAAAAAACTTTTTATAAATAATATATACATACAATGGGTAACAGAGCATTCAAAGC
ACACAACGGTCACTACTTAAGCGCTGGACACGATCACGTCAAGACCCACCACGGTCATCA
CGATCATCACACTCACTTCCATATCGAAAACCACGGAAGCAAAGTTGCCTTAAGAACCCA
TTGCGGTAAATACGTTTCAATTGGTGACCATAAACAAGTTTACCTCTCACACCACTTACA
CGGTGACCACTCACTCTTCCACTTAGAACACCACCACGGTAAAGTCTCAATCAAAGGTCA
TCACCACCACTACATCTCTGTTGACGGTCACGGTCATGTCTCTACCTCCCATCACCACGA
TCATCATGCTACTTTCGAAGAACACATCTTATAAACAAATAAATAAAACAATAAAAATGT
TGTAAAATATTAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAATTTTTTNGTTTTTTTTT
TTNAAAAAAAAAXXXXXXXXXXAATATAAAAAACTTTTTATAAATAATATATACATACAA
TGGGTAACAGAGCATTCAAAGCACACAACGGTCACTACTTAAGCGCTGGACACGATCACG
TCAAGACCCACCACGGTCATCACGATCATCACACTCACTTCCATATCGAAAACCACGGAA
GCAAAGTTGCNTTAAGAACCCATTGCGGTAAATACGTTTCAATTGGTGACCATAAACAAG
TTTACNTCTCACACCACTTACACGGTGACCAATCAATNTTCCACTTAGAACACCACCACG
GTAAAGTNTCAATCAAAGGTCATCACCACCANTACATATCTGTTGACGGTCACGGTCATG
TCTNTACNTCCCATCACCACGATCATCATGCTACNTTCGAAGAACACATCTTATAAACAA
ATAAATAAAANAAAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
niknfl*iiytyng*qsiqstqrsllkrwtrsrqdpprssrsshslpyrkprkqsclknp
lr*irfnw*p*tslpltpltr*pltlplrtppr*slnqrsspplhlc*rsrsclylpspr
sscyfrrthlink*ikq*KCCKILKNKKKKKKKKIFXFFFXKKK---

---YKKLFINNIYIQWVTEHSKHTTVTT*aldtitsrpttvitiitltsisktteaklx*
epiavntfqlvtinkftshttytvtnqxst*ntttvkxqskvittxtylltvtvmsxxpi
ttiimlxskntsykqinkxkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
niknfl*iiytyng*qsiqstqrsllkrwtrsrqdpprssrsshslpyrkprkqsclknp
lr*irfnw*p*tslpltpltr*pltlplrtppr*slnqrsspplhlc*rsrsclylpspr
sscyfrrthlink*ikq*KCCKILKNKKKKKKKKIFXFFFXKKK---

---niknfl*iiytyng*qsiqstqrsllkrwtrsrqdpprssrsshslpyrkprkqscx
knplr*irfnw*p*tslxltpltr*pinxplrtppr*sxnqrssppxhic*rsrsclyxp
sprsscyxrrthlink*ikxkk

Frame B:
i*ktfyk*yihtmgnrafkahnghylsaghdhvkthhghhdhhthfhienhgskvalrth
cgkyvsigdhkqvylshhlhgdhslfhlehhhgkvsikghhhhyisvdghghvstshhhd
hhatfeehil*tnk*nnknvvky*kikkkkkkkkffxfffxkk---

---i*ktfyk*yihtmgnrafkahnghylsaghdhvkthhghhdhhthfhienhgskval
rthcgkyvsigdhkqvyxshhlhgdqsxfhlehhhgkvsikghhhxyisvdghghvxtsh
hhdhhatfeehil*tnk*xkkk

Frame C:
ykklfinniyiqwvtehskhttvtt*aldtitsrpttvitiitltsisktteaklp*epi
avntfqlvtinkftshttytvtthsst*ntttvksqskvittttslltvtvmslppitti
imllskntsykqinktikml*nikk*kkkkkkknfxvfffxkk---

---YKKLFINNIYIQWVTEHSKHTTVTT*aldtitsrpttvitiitltsisktteaklx*
epiavntfqlvtinkftshttytvtnqxst*ntttvkxqskvittxtylltvtvmsxxpi
ttiimlxskntsykqinkxkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE632 (VHE632Q) /CSM/VH/VHE6-B/VHE632Q.Seq.d/                        773   0.0
VFE103 (VFE103Q) /CSM/VF/VFE1-A/VFE103Q.Seq.d/                        765   0.0
VHK281 (VHK281Q) /CSM/VH/VHK2-D/VHK281Q.Seq.d/                        759   0.0
VHH141 (VHH141Q) /CSM/VH/VHH1-B/VHH141Q.Seq.d/                        759   0.0
VFI246 (VFI246Q) /CSM/VF/VFI2-B/VFI246Q.Seq.d/                        759   0.0
VSA785 (VSA785Q) /CSM/VS/VSA7-D/VSA785Q.Seq.d/                        656   0.0
SSG165 (SSG165Q) /CSM/SS/SSG1-C/SSG165Q.Seq.d/                        652   0.0
SSB534 (SSB534Q) /CSM/SS/SSB5-B/SSB534Q.Seq.d/                        634   0.0
VFN807 (VFN807Q) /CSM/VF/VFN8-A/VFN807Q.Seq.d/                        521   e-147
VHL371 (VHL371Q) /CSM/VH/VHL3-C/VHL371Q.Seq.d/                        303   3e-81
own update 2002.12.26
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U13671|U13671.1 Dictyostelium discoideum hisactophilin II (hsII) mRNA, complete cds.   692   0.0    1
J04472|J04472.1 D.discoideum hisactophilin mRNA, complete cds.    268   1e-82  2
AF544403|AF544403.1 Expression vector pNCO113-hisactophilin, complete sequence.   268   2e-78  2
AC091367|AC091367.2 Rattus norvegicus clone CH230-unknown, *** SEQUENCING IN PROGRESS ***, 77 unordered pieces.    38   0.019  2
AC128576|AC128576.3 Rattus norvegicus clone CH230-90O21, WORKING DRAFT SEQUENCE, 3 unordered pieces.    38   0.019  2
AC142140|AC142140.2 Rattus norvegicus clone CH230-393H16, WORKING DRAFT SEQUENCE, 38 unordered pieces.    38   0.020  2
AC180803|AC180803.1 Strongylocentrotus purpuratus clone R3-3071H02, WORKING DRAFT SEQUENCE, 28 unordered pieces.    36   0.27   2
CW212368|CW212368.1 104_643_11190988_116_37025_008 Sorghum methylation filtered library (LibID: 104) Sorghum bicolor genomic clone 11190988, genomic survey sequence.    40   0.31   2
BH989536|BH989536.1 oee42g11.b1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    40   0.37   2
EJ132535|EJ132535.1 1092343572136 Global-Ocean-Sampling_GS-27-01-01-1P8-2P0KB marine metagenome genomic clone 1061001428623 3', genomic survey sequence.    36   0.44   2
dna update 2007. 8.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P42526) RecName: Full=Hisactophilin-2; AltName: Full=Histidine-...   162   2e-38
(P13231) RecName: Full=Hisactophilin-1; AltName: Full=Histidine-...   149   1e-34
AF544403_1(AF544403|pid:none) Expression vector pNCO113-hisactop...   149   1e-34
EF493211_1(EF493211|pid:none) Expression vector pNCO-HISACT-(C49...   145   2e-33
FN322026_1(FN322026|pid:none) Schistosoma japonicum isolate Anhu...    38   0.55
FN322023_1(FN322023|pid:none) Schistosoma japonicum isolate Anhu...    38   0.55
FN322029_1(FN322029|pid:none) Schistosoma japonicum isolate Anhu...    37   0.72
FN322027_1(FN322027|pid:none) Schistosoma japonicum isolate Anhu...    37   0.72
FN322028_1(FN322028|pid:none) Schistosoma japonicum isolate Anhu...    37   0.72
CP000482_2739(CP000482|pid:none) Pelobacter propionicus DSM 2379...    37   0.94
protein update 2009. 6.30
PSORT 

 psg: 0.37  gvh: 0.24  alm: 0.59  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.80  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 84.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: cytoplasmic

>> prediction for VHE632 is nuc
5' end seq. ID VHE632F
5' end seq. 
>VHE632F.Seq
AATATAAAAAACTTTTTATAAATAATATATACATACAATGGGTAACAGAGCATTCAAAGC
ACACAACGGTCACTACTTAAGCGCTGGACACGATCACGTCAAGACCCACCACGGTCATCA
CGATCATCACACTCACTTCCATATCGAAAACCACGGAAGCAAAGTTGCCTTAAGAACCCA
TTGCGGTAAATACGTTTCAATTGGTGACCATAAACAAGTTTACCTCTCACACCACTTACA
CGGTGACCACTCACTCTTCCACTTAGAACACCACCACGGTAAAGTCTCAATCAAAGGTCA
TCACCACCACTACATCTCTGTTGACGGTCACGGTCATGTCTCTACCTCCCATCACCACGA
TCATCATGCTACTTTCGAAGAACACATCTTATAAACAAATAAATAAAACAATAAAAATGT
TGTAAAATATTAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAATTTTTTNGTTTTTTTTT
TTNAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 502
3' end seq. ID VHE632Z
3' end seq. 
>VHE632Z.Seq
NNNNNNNNNNAATATAAAAAACTTTTTATAAATAATATATACATACAATGGGTAACAGAG
CATTCAAAGCACACAACGGTCACTACTTAAGCGCTGGACACGATCACGTCAAGACCCACC
ACGGTCATCACGATCATCACACTCACTTCCATATCGAAAACCACGGAAGCAAAGTTGCNT
TAAGAACCCATTGCGGTAAATACGTTTCAATTGGTGACCATAAACAAGTTTACNTCTCAC
ACCACTTACACGGTGACCAATCAATNTTCCACTTAGAACACCACCACGGTAAAGTNTCAA
TCAAAGGTCATCACCACCANTACATATCTGTTGACGGTCACGGTCATGTCTNTACNTCCC
ATCACCACGATCATCATGCTACNTTCGAAGAACACATCTTATAAACAAATAAATAAAANA
AAAAAAAAA
Length of 3' end seq. 429
Connected seq. ID VHE632P
Connected seq. 
>VHE632P.Seq
AATATAAAAAACTTTTTATAAATAATATATACATACAATGGGTAACAGAGCATTCAAAGC
ACACAACGGTCACTACTTAAGCGCTGGACACGATCACGTCAAGACCCACCACGGTCATCA
CGATCATCACACTCACTTCCATATCGAAAACCACGGAAGCAAAGTTGCCTTAAGAACCCA
TTGCGGTAAATACGTTTCAATTGGTGACCATAAACAAGTTTACCTCTCACACCACTTACA
CGGTGACCACTCACTCTTCCACTTAGAACACCACCACGGTAAAGTCTCAATCAAAGGTCA
TCACCACCACTACATCTCTGTTGACGGTCACGGTCATGTCTCTACCTCCCATCACCACGA
TCATCATGCTACTTTCGAAGAACACATCTTATAAACAAATAAATAAAACAATAAAAATGT
TGTAAAATATTAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAATTTTTTNGTTTTTTTTT
TTNAAAAAAAAA----------AATATAAAAAACTTTTTATAAATAATATATACATACAA
TGGGTAACAGAGCATTCAAAGCACACAACGGTCACTACTTAAGCGCTGGACACGATCACG
TCAAGACCCACCACGGTCATCACGATCATCACACTCACTTCCATATCGAAAACCACGGAA
GCAAAGTTGCNTTAAGAACCCATTGCGGTAAATACGTTTCAATTGGTGACCATAAACAAG
TTTACNTCTCACACCACTTACACGGTGACCAATCAATNTTCCACTTAGAACACCACCACG
GTAAAGTNTCAATCAAAGGTCATCACCACCANTACATATCTGTTGACGGTCACGGTCATG
TCTNTACNTCCCATCACCACGATCATCATGCTACNTTCGAAGAACACATCTTATAAACAA
ATAAATAAAANAAAAAAAAAA
Length of connected seq. 911
Full length Seq ID -
Full length Seq. -
Length of full length seq. -