>VHE611 (VHE611Q) /CSM/VH/VHE6-A/VHE611Q.Seq.d/
TTGGTTTAAGTGCAACATTCATTACTCTCATGCCATCTTACTTAACCCCAATTGGTTGGA
TGTTAATGGGTGCCTCAGTTACTGGTTTAATGGTAATTGGTAATGATTGTATTCATAAAA
CTTTTTCAAAGAATCCATTAGTTAATTCAATTATTGGAACAATTGTAATGTTACCATTGT
TATATCCATTCCAATCTTACAAGATTTCAGAAAAGAATAATGCAGAAAAGAAATCGACAG
CTGTTTTAAATGGTGAACAATTCAAGATTAATGCACATACCACAACCGAAAGTACAAATC
CAATTAGACAATGGGCAACTGGTAAATTCTTTTGGATTTTATCAATTATTAATTGGTCTG
AAAATTATTTCTCAACTAAAAATTTATCAGATAAAAAAGATAAAACCAAAGCAATCGTTT
CAATTGCAATTGTTTACATTTTTGGATTTATTTTCTTCCCAATGATGTTTAAATTTGTAG
GTGTAAGTGGATTCCTTAATTATTGGTTCGCACCATGGTTAGTTTTACATTTCTTACTTT
CAACTGCTTCATTATTACCATCAATTCCATTTTATGAAGAATXXXXXXXXXXATTATCTT
GTTGCTATTTGGTGCAGGTGCTGTCGAAGGTTCATGTCGTTGGTGGTCAAGAGATCATCG
TGCTCATCATCGTTATCTCGATACTGATAAAGATCCATATTCAGCTTCATTGGGTNTCTG
GCATTCCCATTTCATTTGGTTAATGTTCAAACAAGATCCAAAGAAAATCGGTAGAGCAAA
CATTGACGACNTTAACGCCGATCCATGGGTTATGTGGCAACATAAACATTACATTAAAAT
CGCCACTTTTATGGGTCTCGTTTTCCCAACATTGGTAGCTGGTTTAGGTTGGGGTGATTG
GGCTGGTGGTTATTTCTANTCTGGTGTACTTCGTCTCGTTACAGTTCAACATTCCACATT
CTTGGTCAATTCATTGGCTCATTATTTAGGTGACTCTCCATACGATGATGAACATACTCC
AAAGGATTCAGTTGTGACTGCTATATTAACATTTGGTGAAGGTTATCATAACTTCCATCA
TGAATTCCCAAATGACTATAGAAACGCTTATAAATTCTATCAATATGATCCAAATAAATG
GTTGATNTCTGCAATGTATTATTTAGGCTTGGCTTAGATTTGAAAACTTTCTCCAAAAAT
GAAATTGAAAAAGGTAGGATTCAAATGCNCCAAAAACATTTAGACGCAAAGAAACAATCA
ATCTATTGGGGTATCAATAAAGAAGATTT

GLSATFITLMPSYLTPIGWMLMGASVTGLMVIGNDCIHKTFSKNPLVNSIIGTIVMLPLL
YPFQSYKISEKNNAEKKSTAVLNGEQFKINAHTTTESTNPIRQWATGKFFWILSIINWSE
NYFSTKNLSDKKDKTKAIVSIAIVYIFGFIFFPMMFKFVGVSGFLNYWFAPWLVLHFLLS
TASLLPSIPFYEE---

---IILLLFGAGAVEGSCRWWSRDHRAHHRYLDTDKDPYSASLGXWHSHFIWLMFKQDPK
KIGRANIDDXNADPWVMWQHKHYIKIATFMGLVFPTLVAGLGWGDWAGGYFXSGVLRLVT
VQHSTFLVNSLAHYLGDSPYDDEHTPKDSVVTAILTFGEGYHNFHHEFPNDYRNAYKFYQ
YDPNKWLXSAMYYLGLA*i*klspkmklkkvgfkcxkni*tqrnnqsigvsikki

Frame A:
lv*vqhsllschlt*pqlvgc*wvpqllv*w*lvmivfiklfqrih*liqlleql*cyhc
yihsnltrfqkrimqkrnrqlf*mvnnsrlmhipqpkvqiqldngqlvnsfgfyqlligl
kiisqlkiyqikkikpkqsfqlqlftfldlfssq*clnl*v*vdsliigshhg*fyisyf
qllhyyhqfhfmkn---

---IILLLFGAGAVEGSCRWWSRDHRAHHRYLDTDKDPYSASLGXWHSHFIWLMFKQDPK
KIGRANIDDXNADPWVMWQHKHYIKIATFMGLVFPTLVAGLGWGDWAGGYFXSGVLRLVT
VQHSTFLVNSLAHYLGDSPYDDEHTPKDSVVTAILTFGEGYHNFHHEFPNDYRNAYKFYQ
YDPNKWLXSAMYYLGLA*i*klspkmklkkvgfkcxkni*tqrnnqsigvsikki

Frame B:
wfkcnihyshaillnpnwldvngclsywfngnw**lys*nffkesis*fnywnncnvtiv
isipilqdfrke*crkeidscfkw*tiqd*ctyhnrkyksn*tmgnw*illdfiny*lv*
klfln*kfir*kr*nqsnrfncnclhfwiyflpndv*icrckwip*llvrtmvsftfltf
ncfiitinsil*r---

---lsccylvqvlskvhvvggqeiivliivisilikihiqlhwvsgipisfg*csnkiqr
ksveqtlttltpihglcgninitlkspllwvsfsqhw*lv*vgviglvvisxlvyfvslq
fniphswsihwlii*vtlhtmmnilqriql*lly*hlvkviitsimnsqmtietlinsin
miqing*xlqcii*awlrfenflqk*n*kr*dsnapktfrrketinllgyq*rrf

Frame C:
GLSATFITLMPSYLTPIGWMLMGASVTGLMVIGNDCIHKTFSKNPLVNSIIGTIVMLPLL
YPFQSYKISEKNNAEKKSTAVLNGEQFKINAHTTTESTNPIRQWATGKFFWILSIINWSE
NYFSTKNLSDKKDKTKAIVSIAIVYIFGFIFFPMMFKFVGVSGFLNYWFAPWLVLHFLLS
TASLLPSIPFYEE---

---ylvaiwcrccrrfmslvvkrsscssslsry**rsifsfigxlafpfhlvnvqtrske
nr*skh*rx*rrsmgyvat*tlh*nrhfygsrfpnigswfrlg*lgwwlflxwctssrys
stfhilgqfigslfr*lsir**tyskgfscdcyiniw*rls*lps*ipk*l*krl*ilsi
*sk*mvdxcnvlfrlgldlktfskneiekgriqmxqkhldakkqsiywginked

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE611 (VHE611Q) /CSM/VH/VHE6-A/VHE611Q.Seq.d/                       2466   0.0
VHP282 (VHP282Q) /CSM/VH/VHP2-D/VHP282Q.Seq.d/                       1322   0.0
VFM428 (VFM428Q) /CSM/VF/VFM4-B/VFM428Q.Seq.d/                       1314   0.0
VHA478 (VHA478Q) /CSM/VH/VHA4-D/VHA478Q.Seq.d/                       1154   0.0
VHC459 (VHC459Q) /CSM/VH/VHC4-C/VHC459Q.Seq.d/                       1142   0.0
VHH531 (VHH531Q) /CSM/VH/VHH5-B/VHH531Q.Seq.d/                       1114   0.0
VFB582 (VFB582Q) /CSM/VF/VFB5-D/VFB582Q.Seq.d/                       1106   0.0
VHD470 (VHD470Q) /CSM/VH/VHD4-C/VHD470Q.Seq.d/                       1078   0.0
VHI633 (VHI633Q) /CSM/VH/VHI6-B/VHI633Q.Seq.d/                       1074   0.0
VHP359 (VHP359Q) /CSM/VH/VHP3-C/VHP359Q.Seq.d/                       1072   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.  1126   0.0    2
DY886427|DY886427.1 CeleSEQ2079 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ2079, mRNA sequence.    68   6e-24  4
DY892961|DY892961.1 CeleSEQ12104 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ12104, mRNA sequence.    68   7e-24  4
DY888809|DY888809.1 CeleSEQ6138 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ6138, mRNA sequence.    68   9e-23  4
DY885119|DY885119.1 CeleSEQ310 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ310, mRNA sequence.    68   6e-16  3
DY885952|DY885952.1 CeleSEQ1408 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ1408, mRNA sequence.    68   8e-16  3
DY885558|DY885558.1 CeleSEQ846 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ846, mRNA sequence.    68   1e-15  3
DY890339|DY890339.1 CeleSEQ9316 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ9316, mRNA sequence.    68   3e-15  3
DY885117|DY885117.1 CeleSEQ308 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ308, mRNA sequence.    52   1e-13  3
DY895351|DY895351.1 CeleSEQ15152 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ15152, mRNA sequence.    52   1e-13  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116984_117(AC116984|pid:none) Dictyostelium discoideum chromos...   416   e-115
AY576801_1(AY576801|pid:none) Hortaea werneckii putative delta-9...   285   2e-82
DQ901954_1(DQ901954|pid:none) Aureobasidium pullulans putative d...   281   9e-81
S52746(S52746;S52745) stearoyl-CoA 9-desaturase (EC 1.14.19.1) [...   279   6e-80
(Q12618) RecName: Full=Acyl-CoA desaturase;          EC=1.14.19....   277   1e-79
AM920435_140(AM920435|pid:none) Penicillium chrysogenum Wisconsi...   277   1e-79
AP007162_283(AP007162|pid:none) Aspergillus oryzae RIB40 genomic...   272   3e-79
AF510861_1(AF510861|pid:none) Emericella nidulans stearic acid d...   271   5e-78
AP007151_392(AP007151|pid:none) Aspergillus oryzae RIB40 genomic...   271   5e-78
Y18554_1(Y18554|pid:none) Mortierella alpina ole2 gene for stear...   290   6e-77

 psg: 0.94  gvh: 0.44  alm: 0.22  top: 0.57  tms: 0.40  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.50
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 52.0 %: plasma membrane
	 16.0 %: endoplasmic reticulum
	 12.0 %: vacuolar
	  8.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: cytoplasmic
	  4.0 %: nuclear

>> prediction for VHE611 is pla

>VHE611F.Seq
TTGGTTTAAGTGCAACATTCATTACTCTCATGCCATCTTACTTAACCCCAATTGGTTGGA
TGTTAATGGGTGCCTCAGTTACTGGTTTAATGGTAATTGGTAATGATTGTATTCATAAAA
CTTTTTCAAAGAATCCATTAGTTAATTCAATTATTGGAACAATTGTAATGTTACCATTGT
TATATCCATTCCAATCTTACAAGATTTCAGAAAAGAATAATGCAGAAAAGAAATCGACAG
CTGTTTTAAATGGTGAACAATTCAAGATTAATGCACATACCACAACCGAAAGTACAAATC
CAATTAGACAATGGGCAACTGGTAAATTCTTTTGGATTTTATCAATTATTAATTGGTCTG
AAAATTATTTCTCAACTAAAAATTTATCAGATAAAAAAGATAAAACCAAAGCAATCGTTT
CAATTGCAATTGTTTACATTTTTGGATTTATTTTCTTCCCAATGATGTTTAAATTTGTAG
GTGTAAGTGGATTCCTTAATTATTGGTTCGCACCATGGTTAGTTTTACATTTCTTACTTT
CAACTGCTTCATTATTACCATCAATTCCATTTTATGAAGAATNNNNNNNNNN

>VHE611Z.Seq
NNNNNNNNNNATTATCTTGTTGCTATTTGGTGCAGGTGCTGTCGAAGGTTCATGTCGTTG
GTGGTCAAGAGATCATCGTGCTCATCATCGTTATCTCGATACTGATAAAGATCCATATTC
AGCTTCATTGGGTNTCTGGCATTCCCATTTCATTTGGTTAATGTTCAAACAAGATCCAAA
GAAAATCGGTAGAGCAAACATTGACGACNTTAACGCCGATCCATGGGTTATGTGGCAACA
TAAACATTACATTAAAATCGCCACTTTTATGGGTCTCGTTTTCCCAACATTGGTAGCTGG
TTTAGGTTGGGGTGATTGGGCTGGTGGTTATTTCTANTCTGGTGTACTTCGTCTCGTTAC
AGTTCAACATTCCACATTCTTGGTCAATTCATTGGCTCATTATTTAGGTGACTCTCCATA
CGATGATGAACATACTCCAAAGGATTCAGTTGTGACTGCTATATTAACATTTGGTGAAGG
TTATCATAACTTCCATCATGAATTCCCAAATGACTATAGAAACGCTTATAAATTCTATCA
ATATGATCCAAATAAATGGTTGATNTCTGCAATGTATTATTTAGGCTTGGCTTAGATTTG
AAAACTTTCTCCAAAAATGAAATTGAAAAAGGTAGGATTCAAATGCNCCAAAAACATTTA
GACGCAAAGAAACAATCAATCTATTGGGGTATCAATAAAGAAGATTT

>VHE611P.Seq
TTGGTTTAAGTGCAACATTCATTACTCTCATGCCATCTTACTTAACCCCAATTGGTTGGA
TGTTAATGGGTGCCTCAGTTACTGGTTTAATGGTAATTGGTAATGATTGTATTCATAAAA
CTTTTTCAAAGAATCCATTAGTTAATTCAATTATTGGAACAATTGTAATGTTACCATTGT
TATATCCATTCCAATCTTACAAGATTTCAGAAAAGAATAATGCAGAAAAGAAATCGACAG
CTGTTTTAAATGGTGAACAATTCAAGATTAATGCACATACCACAACCGAAAGTACAAATC
CAATTAGACAATGGGCAACTGGTAAATTCTTTTGGATTTTATCAATTATTAATTGGTCTG
AAAATTATTTCTCAACTAAAAATTTATCAGATAAAAAAGATAAAACCAAAGCAATCGTTT
CAATTGCAATTGTTTACATTTTTGGATTTATTTTCTTCCCAATGATGTTTAAATTTGTAG
GTGTAAGTGGATTCCTTAATTATTGGTTCGCACCATGGTTAGTTTTACATTTCTTACTTT
CAACTGCTTCATTATTACCATCAATTCCATTTTATGAAGAAT----------ATTATCTT
GTTGCTATTTGGTGCAGGTGCTGTCGAAGGTTCATGTCGTTGGTGGTCAAGAGATCATCG
TGCTCATCATCGTTATCTCGATACTGATAAAGATCCATATTCAGCTTCATTGGGTNTCTG
GCATTCCCATTTCATTTGGTTAATGTTCAAACAAGATCCAAAGAAAATCGGTAGAGCAAA
CATTGACGACNTTAACGCCGATCCATGGGTTATGTGGCAACATAAACATTACATTAAAAT
CGCCACTTTTATGGGTCTCGTTTTCCCAACATTGGTAGCTGGTTTAGGTTGGGGTGATTG
GGCTGGTGGTTATTTCTANTCTGGTGTACTTCGTCTCGTTACAGTTCAACATTCCACATT
CTTGGTCAATTCATTGGCTCATTATTTAGGTGACTCTCCATACGATGATGAACATACTCC
AAAGGATTCAGTTGTGACTGCTATATTAACATTTGGTGAAGGTTATCATAACTTCCATCA
TGAATTCCCAAATGACTATAGAAACGCTTATAAATTCTATCAATATGATCCAAATAAATG
GTTGATNTCTGCAATGTATTATTTAGGCTTGGCTTAGATTTGAAAACTTTCTCCAAAAAT
GAAATTGAAAAAGGTAGGATTCAAATGCNCCAAAAACATTTAGACGCAAAGAAACAATCA
ATCTATTGGGGTATCAATAAAGAAGATTT