NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE591
Library VH
(Link to library)
Clone ID VHE591
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHE591P
(Link to Original site)
Representative DNA sequence 
>VHE591 (VHE591Q) /CSM/VH/VHE5-D/VHE591Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGGTATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAXXXXXXXXXXACTTAGTAG
AAATTACTGCCCCAGAAAANGNTANTGGTGGTATTTANTCTGNAATCAATCGTCGTANAG
GTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGNTCATT
TACCAGTATTAGAATCCTTCGGTTTCACCGCTGANTTACGNTCCCACACTGCCGGTCAAG
NTTTCCCACAATGTGTNTTCGATCANTGGGNNTCAATTGGTGTTGTCAACAAAGATAAGA
AAGCCNCTGAAGTCGNTTTAGCCNCCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAG
ATCTTGACAAANTCCNCGAAAAACTTTAAACAATTTAAGTCGCA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQ---

---LVEITAPEXXXGGIXSXINRRXGIVIGEERRIGSPLLSVKXHLPVLESFGFTAXLRS
HTAGQXFPQCVFDXWXSIGVVNKDKKAXEVXLAXRKRKGLAPEIPDLDKXXEKL*ti*va
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*gichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyv---

---t**kllpqkxxxvvfxlxsivvxvlllvknvvsvphyylskxiyqy*npsvsplxyx
ptlpvkxshnvxsixgxqlvlstkirkplksx*pperekv*pqksqiltxsxknfkqfks

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yevyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncits---

---lsrnycprkxxwwylxxnqssxrycyw*rtsyrfpiiicqxsftsirilrfhr*xtx
phcrsxfptmcxrsxgxnwccqqr*esx*srfsxqkekrfsprnprs*qxprktlnnlsr

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQ---

---LVEITAPEXXXGGIXSXINRRXGIVIGEERRIGSPLLSVKXHLPVLESFGFTAXLRS
HTAGQXFPQCVFDXWXSIGVVNKDKKAXEVXLAXRKRKGLAPEIPDLDKXXEKL*ti*va
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE591 (VHE591Q) /CSM/VH/VHE5-D/VHE591Q.Seq.d/                       1421   0.0
VHP828 (VHP828Q) /CSM/VH/VHP8-B/VHP828Q.Seq.d/                        874   0.0
VHL845 (VHL845Q) /CSM/VH/VHL8-B/VHL845Q.Seq.d/                        874   0.0
VHJ805 (VHJ805Q) /CSM/VH/VHJ8-A/VHJ805Q.Seq.d/                        874   0.0
VHG231 (VHG231Q) /CSM/VH/VHG2-B/VHG231Q.Seq.d/                        874   0.0
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/             866   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                        866   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                        866   0.0
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/             866   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                        866   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.   864   0.0    3
DY889811|DY889811.1 CeleSEQ6761 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ6761, mRNA sequence.    84   4e-30  4
DY889772|DY889772.1 CeleSEQ11980 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ11980, mRNA sequence.    84   4e-30  4
DY890935|DY890935.1 CeleSEQ7425 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ7425, mRNA sequence.    84   6e-30  4
DY887441|DY887441.1 CeleSEQ3766 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ3766, mRNA sequence.    84   6e-30  4
DY888936|DY888936.1 CeleSEQ6366 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ6366, mRNA sequence.    84   9e-30  4
DY891613|DY891613.1 CeleSEQ10349 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ10349, mRNA sequence.    84   2e-27  3
AF534908|AF534908.2 Tetrahymena thermophila elongation factor 2 gene, complete cds.    84   5e-27  3
AF107291|AF107291.1 Candida parapsilosis elongation factor 2 (EFT2) gene, partial cds.    56   6e-27  8
DY889006|DY889006.1 CeleSEQ6474 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ6474, mRNA sequence.    68   3e-26  4
dna update 2007. 8.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   207   3e-52
AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   206   6e-52
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   204   3e-51
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   202   1e-50
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   201   2e-50
CP000881_15(CP000881|pid:none) Hemiselmis andersenii chromosome ...   201   2e-50
AP005845_16(AP005845|pid:none) Oryza sativa Japonica Group genom...   199   7e-50
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   199   9e-50
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   199   9e-50
AY618550_1(AY618550|pid:none) Trypanosoma cruzi elongation facto...   199   1e-49
protein update 2009. 6.30
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHE591 is cyt
5' end seq. ID VHE591F
5' end seq. 
>VHE591F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGGTATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAANNNNNNNNNN
Length of 5' end seq. 471
3' end seq. ID VHE591Z
3' end seq. 
>VHE591Z.Seq
NNNNNNNNNNACTTAGTAGAAATTACTGCCCCAGAAAANGNTANTGGTGGTATTTANTCT
GNAATCAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTA
TTATCTGTCAAAGNTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGANTTACGN
TCCCACACTGCCGGTCAAGNTTTCCCACAATGTGTNTTCGATCANTGGGNNTCAATTGGT
GTTGTCAACAAAGATAAGAAAGCCNCTGAAGTCGNTTTAGCCNCCAGAAAGAGAAAAGGT
TTAGCCCCAGAAATCCCAGATCTTGACAAANTCCNCGAAAAACTTTAAACAATTTAAGTC
GCA
Length of 3' end seq. 363
Connected seq. ID VHE591P
Connected seq. 
>VHE591P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGGTATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAA----------ACTTAGTAG
AAATTACTGCCCCAGAAAANGNTANTGGTGGTATTTANTCTGNAATCAATCGTCGTANAG
GTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGNTCATT
TACCAGTATTAGAATCCTTCGGTTTCACCGCTGANTTACGNTCCCACACTGCCGGTCAAG
NTTTCCCACAATGTGTNTTCGATCANTGGGNNTCAATTGGTGTTGTCAACAAAGATAAGA
AAGCCNCTGAAGTCGNTTTAGCCNCCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAG
ATCTTGACAAANTCCNCGAAAAACTTTAAACAATTTAAGTCGCA
Length of connected seq. 814
Full length Seq ID -
Full length Seq. -
Length of full length seq. -