>VHE586 (VHE586Q) /CSM/VH/VHE5-D/VHE586Q.Seq.d/
TAATGGAAAAAAAAAACAAAAATAAATGATATTAAAAAATCAAAACCAAAACTATCATCA
ACATTATTAAATGTTCATATTGTTGCTCATACTCATGATGATGTTGGTTGGTTAAAAACA
GTTGATGAATATTATTATGGATCAAATATGTCAATTGCATTTGCAGGTGCTCAATATACT
TTGGATACAGCCATTACCTGTTTATTGGCAAATCCANAAAGAAAGTTTATTTATGTTGAA
ATTGCATTTTTCCAAAGATGGTGGGATGAACAAAGTACAACAATGCAAAATATTGTTAAG
GGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAATGGTGGGTATTGTATGAATGATGAG
GCTACTACCTACTATGACGATACTATTGACCAAATGACATTGGGCCATCAATTCCTTTGG
GANAATTTCGGAGTTATGCCAAANATAGGCTGGCNCATANATCCATTTGGCCATAGTGCA
ACACAAGCTCGTATCTTTGGTCAATTAGGATTCGATGCATTCATTATCGGTCGTATGGAT
TATTXXXXXXXXXXGTGGTTCAACCAACCAGTGGTAATTATGTACNTGTGAATGCTATTG
CTTACATTCAAGATCTTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTG
GATGTGCATCGNTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAG
ATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTA
AATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTNT
CTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGA
ATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCNTCACNTTTACCAAATGGTT
TAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTG
AAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATC
TTTCAACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTG
TTCAAAAATTATCAAATTTATCAAG

*WKKKTKINDIKKSKPKLSSTLLNVHIVAHTHDDVGWLKTVDEYYYGSNMSIAFAGAQYT
LDTAITCLLANPXRKFIYVEIAFFQRWWDEQSTTMQNIVKGLVESGQLEFINGGYCMNDE
ATTYYDDTIDQMTLGHQFLWXNFGVMPXIGWXIXPFGHSATQARIFGQLGFDAFIIGRMD
Y---

---VVQPTSGNYVXVNAIAYIQDLNQSLQFTIVTDRSRGCASXRDGQLDMMMHRRTLKDD
GRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNS
QYQGVYSPLTSASXLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLS
TIFSTITITSATEMNLTGVQKLSNLS

Frame A:
*WKKKTKINDIKKSKPKLSSTLLNVHIVAHTHDDVGWLKTVDEYYYGSNMSIAFAGAQYT
LDTAITCLLANPXRKFIYVEIAFFQRWWDEQSTTMQNIVKGLVESGQLEFINGGYCMNDE
ATTYYDDTIDQMTLGHQFLWXNFGVMPXIGWXIXPFGHSATQARIFGQLGFDAFIIGRMD
Y---

---VVQPTSGNYVXVNAIAYIQDLNQSLQFTIVTDRSRGCASXRDGQLDMMMHRRTLKDD
GRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNS
QYQGVYSPLTSASXLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLS
TIFSTITITSATEMNLTGVQKLSNLS

Frame B:
ngkkkqk*milknqnqnyhqhy*mfilllilmmmlvg*kqlmniimdqicqlhlqvlnil
wiqplpvywqixkeslfmlklhfskdggmnkvqqckillrvwlkvvnwns*mvgiv*mmr
llptmtilltk*hwainsfgxiselcqx*agxxihlaivqhklvslvn*dsmhslsvvwi
i---

---wfnqpvvimyx*mllltfkilinhynsplslievvdvhr*emvnwi**civvl*kmm
vvvlvnq*msqlkslqlvn*fsmifqvmlnliivllhxlyhihysqclqplnnlqtigil
nikvfthh*lqxhxyqmv*rfklyng*iikiipyfyvlkifiklmvkihkihklsl*ifq
lsfqqlqsllqlk*i*lvfknyqiyq

Frame C:
mekknknk*y*kiktktiiniikcsyccsys**cwlvkns**illwikyvncicrcsiyf
gyshylfigksxkkvylc*ncifpkmvg*tkynnakyc*gfg*kwsigihkwwvlye**g
yyll*ryy*pndigpsiplgxfrsyakxrlahxsiwp*cntssylwsirircihyrsygl
---

---gstnqw*lctcecyclhsrs*siitihhch**kswmcixkrwsigyddassyfer*w
swcwstne*vnsnryn**idfp*yfklcsislsscctxfitsitpnvynhstifkrlefs
isrclltidfsxtftkwfkdsnstmvr*sr*yhtfty*kylsn*wsrftrstnyhfrsfn
ylfnnynhfcn*nefnwcskiikfik

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE586 (VHE586Q) /CSM/VH/VHE5-D/VHE586Q.Seq.d/                       2089   0.0
CHQ142 (CHQ142Q) /CSM/CH/CHQ1-B/CHQ142Q.Seq.d/                       1179   0.0
VHE761 (VHE761Q) /CSM/VH/VHE7-C/VHE761Q.Seq.d/                       1176   0.0
SHF209 (SHF209Q) /CSM/SH/SHF2-A/SHF209Q.Seq.d/                       1176   0.0
SHK860 (SHK860Q) /CSM/SH/SHK8-C/SHK860Q.Seq.d/                       1170   0.0
SHF812 (SHF812Q) /CSM/SH/SHF8-A/SHF812Q.Seq.d/                       1170   0.0
VHP767 (VHP767Q) /CSM/VH/VHP7-C/VHP767Q.Seq.d/                       1168   0.0
VHB838 (VHB838Q) /CSM/VH/VHB8-B/VHB838Q.Seq.d/                       1168   0.0
SHE879 (SHE879Q) /CSM/SH/SHE8-D/SHE879Q.Seq.d/                       1168   0.0
SHE384 (SHE384Q) /CSM/SH/SHE3-D/SHE384Q.Seq.d/                       1168   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1140   0.0    1
CK149800|CK149800.1 CsmgEST00336 Culicoides sonorensis female serum-fed midgut cDNA Library Culicoides sonorensis cDNA, mRNA sequence.   117   1e-23  3
CB073433|CB073433.1 taa32h01.y1 Hydra EST -II Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence.    52   2e-13  3
DN814237|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   2e-13  3
DN816506|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   2e-13  3
CV682907|CV682907.1 service43_H06_sjs2-16-2B11-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    74   3e-13  2
CX633815|CX633815.1 taj44a03.y3 Hydra EST UCI 5 Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence.    52   3e-13  3
CV688215|CV688215.1 sjs2-16-2_G01_sjs2-16-2G01-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    74   3e-13  2
CV682854|CV682854.1 service30_G09_sjs2-14-10C09-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    74   3e-13  2
DN816232| truncated lysosomal acid alpha-mannosidase [Homo sapiens], mRNA sequence.    52   3e-13  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   325   2e-87
(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   325   2e-87
AK068415_1(AK068415|pid:none) Oryza sativa Japonica Group cDNA c...   206   2e-51
AY099704_1(AY099704|pid:none) Arabidopsis thaliana alpha-mannosi...   206   2e-51
AB005230_20(AB005230|pid:none) Arabidopsis thaliana genomic DNA,...   206   2e-51
(Q29451) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   204   6e-51
AK291572_1(AK291572|pid:none) Homo sapiens cDNA FLJ76867 complet...   203   8e-51
U05572_1(U05572|pid:none) Human lysosomal alpha-mannosidase (MAN...   203   8e-51
AB209921_1(AB209921|pid:none) Homo sapiens mRNA for mannosidase,...   203   8e-51
AB026648_12(AB026648|pid:none) Arabidopsis thaliana genomic DNA,...   203   8e-51

 psg: 0.53  gvh: 0.43  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHE586 is cyt

>VHE586F.Seq
TAATGGAAAAAAAAAACAAAAATAAATGATATTAAAAAATCAAAACCAAAACTATCATCA
ACATTATTAAATGTTCATATTGTTGCTCATACTCATGATGATGTTGGTTGGTTAAAAACA
GTTGATGAATATTATTATGGATCAAATATGTCAATTGCATTTGCAGGTGCTCAATATACT
TTGGATACAGCCATTACCTGTTTATTGGCAAATCCANAAAGAAAGTTTATTTATGTTGAA
ATTGCATTTTTCCAAAGATGGTGGGATGAACAAAGTACAACAATGCAAAATATTGTTAAG
GGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAATGGTGGGTATTGTATGAATGATGAG
GCTACTACCTACTATGACGATACTATTGACCAAATGACATTGGGCCATCAATTCCTTTGG
GANAATTTCGGAGTTATGCCAAANATAGGCTGGCNCATANATCCATTTGGCCATAGTGCA
ACACAAGCTCGTATCTTTGGTCAATTAGGATTCGATGCATTCATTATCGGTCGTATGGAT
TATTNNNNNNNNNN

>VHE586Z.Seq
NNNNNNNNNNGTGGTTCAACCAACCAGTGGTAATTATGTACNTGTGAATGCTATTGCTTA
CATTCAAGATCTTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATG
TGCATCGNTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGA
TGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATT
GATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTNTCTTT
ATCACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTC
TCAATATCAAGGTGTTTACTCACCATTGACTTCAGCNTCACNTTTACCAAATGGTTTAAA
GATTCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAA
TATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTC
AACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCA
AAAATTATCAAATTTATCAAG

>VHE586P.Seq
TAATGGAAAAAAAAAACAAAAATAAATGATATTAAAAAATCAAAACCAAAACTATCATCA
ACATTATTAAATGTTCATATTGTTGCTCATACTCATGATGATGTTGGTTGGTTAAAAACA
GTTGATGAATATTATTATGGATCAAATATGTCAATTGCATTTGCAGGTGCTCAATATACT
TTGGATACAGCCATTACCTGTTTATTGGCAAATCCANAAAGAAAGTTTATTTATGTTGAA
ATTGCATTTTTCCAAAGATGGTGGGATGAACAAAGTACAACAATGCAAAATATTGTTAAG
GGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAATGGTGGGTATTGTATGAATGATGAG
GCTACTACCTACTATGACGATACTATTGACCAAATGACATTGGGCCATCAATTCCTTTGG
GANAATTTCGGAGTTATGCCAAANATAGGCTGGCNCATANATCCATTTGGCCATAGTGCA
ACACAAGCTCGTATCTTTGGTCAATTAGGATTCGATGCATTCATTATCGGTCGTATGGAT
TATT----------GTGGTTCAACCAACCAGTGGTAATTATGTACNTGTGAATGCTATTG
CTTACATTCAAGATCTTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTG
GATGTGCATCGNTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAG
ATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTA
AATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTNT
CTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGA
ATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCNTCACNTTTACCAAATGGTT
TAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTG
AAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATC
TTTCAACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTG
TTCAAAAATTATCAAATTTATCAAG