>VHE526 (VHE526Q) /CSM/VH/VHE5-B/VHE526Q.Seq.d/
AAAAACCACCAGTTGAACCAGTTGTAGTTCAAAAATCACCAGTTGTTCAACAACAACCAA
CAACATCAACTTATAAACATGTATTTAATCCAGTACAACAACAACAACAACAAACTACTC
AATCTACAACAGTTAAACAACCAGAAATACCAAAAGTTGTAGAAGAACCAAAGAAAGTAG
AACCAACACCAACACCAAAACCATTACCAAAACCAGAACCAACAATTGTTATTCCAACTC
AAGTTAAACAACCAGAAATACCAAAAGTTGTAGAAGAACCAAAGAAAGTAGAACAAGTTT
CAATTATTAAAACTGATGAAAATGTTCAAACATTAGCACCATTACCAGAGAAAGCTGAAG
GTGTTAAAAGACAAGCTGCATCTAGAAATCCAATTAGAGCTAGACAAGAAAGAGCTGCTC
AAGAAGAAGCAGATGCAAAGAAAAGATTGGAAGAACAAGCCAAAGCTAATGCATCATCAA
CAACAACAACTGTAACAGTTACAAATTCAAAACCAAATGTTCAATCAGCATTAGGTGGAT
TTGGATTATTATATTCAGTTGGAGCACCAGATGAAACAATGTTTAAAACAAGAGCTGAAA
GAATTCAATCAGCAATGGATAATTCATCATCATCAAATCCATTCTCATTAAATCAXXXXX
XXXXXTCAAAGAATATNTCATCGAAATCTTTAAATAGCAATGGTAGATNTAAATTGGTTG
AATTTGAAGAAGGTAAAGAATCTGAAGAATTCTTTAAACTTTTAGGTGGTCGTCAACCAT
ATCCATTATCAAAATCCACTTCAAGAGTTGAACCACGTCTTTATCATTGTACTGTTGGTT
CTGGTGCTTTTGTTGTAGATGAAGTCACTTCTTTTGCTCAAGAGGATTTACTTCAAGAGG
ATGTTTTCATCGTTGATGGTATCGAACAAATTTTCATTTGGATCGGTACTGAAACCACTG
AAACTGAACGTAAATCTTCAATGGAAGTAGCAGTTGAATACTCTTCAACTTTACCATCAC
CAAGAAAACAAAATATTCCCGTCTATCTCACTTATCATGGTAAAGAACCTTACATTTTCA
CAAGCCTATTCCATGGTTGGGACTTTTCAAAGAGAGTCATTCCAACTATCTNTCTTGATC
AAGATTTACTTTTAGCAAAGGAAATATTAGAACTTTATACTAAAAAATATACATATGATC
AATTAGTAAAGAAACAATATCCAAAAGGTATAGATGGTTCTCGTTTAGAAGAATATTTAT
CTGATGAAGAATTCATTTCAATTTTCAAGATAACTCTTGCTCAATTTAAAACTTTATCTT
T

KPPVEPVVVQKSPVVQQQPTTSTYKHVFNPVQQQQQQTTQSTTVKQPEIPKVVEEPKKVE
PTPTPKPLPKPEPTIVIPTQVKQPEIPKVVEEPKKVEQVSIIKTDENVQTLAPLPEKAEG
VKRQAASRNPIRARQERAAQEEADAKKRLEEQAKANASSTTTTVTVTNSKPNVQSALGGF
GLLYSVGAPDETMFKTRAERIQSAMDNSSSSNPFSLN---

---SKNXSSKSLNSNGRXKLVEFEEGKESEEFFKLLGGRQPYPLSKSTSRVEPRLYHCTV
GSGAFVVDEVTSFAQEDLLQEDVFIVDGIEQIFIWIGTETTETERKSSMEVAVEYSSTLP
SPRKQNIPVYLTYHGKEPYIFTSLFHGWDFSKRVIPTIXLDQDLLLAKEILELYTKKYTY
DQLVKKQYPKGIDGSRLEEYLSDEEFISIFKITLAQFKTLS

Frame A:
knhqlnql*fknhqlfnnnqqhqlinmyliqynnnnnkllnlqqlnnqkyqkl*knqrk*
nqhqhqnhyqnqnqqllfqlklnnqkyqkl*knqrk*nkfqllklmkmfkh*hhyqrklk
vlkdklhleiqleldkkellkkkqmqrkdwknkpklmhhqqqql*qlqiqnqmfnqh*vd
ldyyiqlehqmkqclkqelkefnqqwiihhhqihsh*i---

---SKNXSSKSLNSNGRXKLVEFEEGKESEEFFKLLGGRQPYPLSKSTSRVEPRLYHCTV
GSGAFVVDEVTSFAQEDLLQEDVFIVDGIEQIFIWIGTETTETERKSSMEVAVEYSSTLP
SPRKQNIPVYLTYHGKEPYIFTSLFHGWDFSKRVIPTIXLDQDLLLAKEILELYTKKYTY
DQLVKKQYPKGIDGSRLEEYLSDEEFISIFKITLAQFKTLS

Frame B:
ktts*tscsskitscstttnninl*tci*ssttttttnysiyns*ttrntkscrrtkesr
tntntktitktrtnncysnss*ttrntkscrrtkesrtsfny*n**kcsnistitres*r
c*ktsci*ksn*s*trkscsrrsrckekigrtsqs*ciinnnncnsykfktkcsisirwi
wiiifswstr*nnv*nks*knsisng*fiiiksiliks---

---qrixhrnl*iamvdxnwlnlkkvknlknslnf*vvvnhihyqnplqelnhvfiivll
vlvlll*mksllllkriyfkrmfsslmvsnkfsfgsvlkplklnvnlqwk*qlntlqlyh
hqenkifpsislimvknltfsqaysmvgtfqresfqlsxlikiyf*qrky*nfilknihm
in**rnniqkv*mvlv*kniylmknsfqfsr*lllnlklyl

Frame C:
KPPVEPVVVQKSPVVQQQPTTSTYKHVFNPVQQQQQQTTQSTTVKQPEIPKVVEEPKKVE
PTPTPKPLPKPEPTIVIPTQVKQPEIPKVVEEPKKVEQVSIIKTDENVQTLAPLPEKAEG
VKRQAASRNPIRARQERAAQEEADAKKRLEEQAKANASSTTTTVTVTNSKPNVQSALGGF
GLLYSVGAPDETMFKTRAERIQSAMDNSSSSNPFSLN---

---keyxieifk*qw*x*ig*i*rr*ri*ril*tfrwsstisiikihfks*ttslslycw
fwcfccr*shffcsrgftsrgcfhr*wyrtnfhldry*nh*n*t*ifngsss*ilfnfti
tkktkysrlshlsw*rtlhfhkpipwlglfkeshsnyls*srftfskgnirtly*kiyi*
sisketiskryrwfsfrrifi**rihfnfqdnscsi*nfif

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE526 (VHE526Q) /CSM/VH/VHE5-B/VHE526Q.Seq.d/                       2099   0.0
VHI673 (VHI673Q) /CSM/VH/VHI6-D/VHI673Q.Seq.d/                       1267   0.0
SHA526 (SHA526Q) /CSM/SH/SHA5-B/SHA526Q.Seq.d/                       1267   0.0
CHL780 (CHL780Q) /CSM/CH/CHL7-D/CHL780Q.Seq.d/                       1267   0.0
AHO473 (AHO473Q) /CSM/AH/AHO4-D/AHO473Q.Seq.d/                       1267   0.0
AHM353 (AHM353Q) /CSM/AH/AHM3-C/AHM353Q.Seq.d/                       1267   0.0
AHM312 (AHM312Q) /CSM/AH/AHM3-A/AHM312Q.Seq.d/                       1267   0.0
AHE220 (AHE220Q) /CSM/AH/AHE2-A/AHE220Q.Seq.d/                       1267   0.0
AHD670 (AHD670Q) /CSM/AH/AHD6-C/AHD670Q.Seq.d/                       1267   0.0
AFO518 (AFO518Q) /CSM/AF/AFO5-A/AFO518Q.Seq.d/                       1253   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U23477|U23477.1 Dictyostelium discoideum phosphatidylinositol-4,5-diphosphate 3-kinase (PIK2) mRNA, complete cds.    32   0.004  5
DU520572|DU520572.1 1098609846775 CHORI-243 Ovis aries genomic clone CH243-165I16, genomic survey sequence.    52   0.044  1
AC115680|AC115680.3 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.    46   0.096  7
DR952216|DR952216.1 EST1143755 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1RZ58, mRNA sequence.    50   0.17   1
BV555881|BV555881.1 S222P626FF5.T0 Karlien Pan troglodytes verus STS genomic, sequence tagged site.    50   0.17   1
AC027226|AC027226.2 Homo sapiens chromosome 21 clone RP11-709I9 map 21, WORKING DRAFT SEQUENCE, 25 unordered pieces.    50   0.17   1
AG017600|AG017600.2 Homo sapiens genomic DNA, 21q region, clone: B42M17SG28b011.    50   0.17   1
BS000087|BS000087.1 Pan troglodytes chromosome 22 clone:RP43-110O22, map 22, complete sequences.    50   0.17   1
AP001687|AP001687.1 Homo sapiens genomic DNA, chromosome 21q, section 31/105.    50   0.17   1
AP000459|AP000459.4 Homo sapiens genomic DNA, chromosome 21q, clone:RP11-42M17.    50   0.17   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC135895_1(BC135895|pid:none) Xenopus tropicalis hypothetical pr...   112   2e-23
BC111730_1(BC111730|pid:none) Homo sapiens advillin, mRNA (cDNA ...   110   1e-22
(O75366) RecName: Full=Advillin; AltName: Full=p92;  &AK314362_1...   110   1e-22
AF041449_1(AF041449|pid:none) Homo sapiens advillin mRNA, comple...   110   1e-22
AK154851_1(AK154851|pid:none) Mus musculus NOD-derived CD11c +ve...   109   3e-22
AF099929_1(AF099929|pid:none) Rattus norvegicus pervin mRNA, com...   108   4e-22
BC054960_1(BC054960|pid:none) Xenopus laevis villin 1, mRNA (cDN...   107   7e-22
BC168981_1(BC168981|pid:none) Rattus norvegicus villin 1, mRNA (...   103   1e-20
(P09327) RecName: Full=Villin-1;                                      103   1e-20
A07400_1(A07400|pid:none) H.sapiens DNA for villin.  &A31642(A31...   103   1e-20

 psg: 0.70  gvh: 0.42  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.36  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: peroxisomal
	  4.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VHE526 is cyt

>VHE526F.Seq
AAAAACCACCAGTTGAACCAGTTGTAGTTCAAAAATCACCAGTTGTTCAACAACAACCAA
CAACATCAACTTATAAACATGTATTTAATCCAGTACAACAACAACAACAACAAACTACTC
AATCTACAACAGTTAAACAACCAGAAATACCAAAAGTTGTAGAAGAACCAAAGAAAGTAG
AACCAACACCAACACCAAAACCATTACCAAAACCAGAACCAACAATTGTTATTCCAACTC
AAGTTAAACAACCAGAAATACCAAAAGTTGTAGAAGAACCAAAGAAAGTAGAACAAGTTT
CAATTATTAAAACTGATGAAAATGTTCAAACATTAGCACCATTACCAGAGAAAGCTGAAG
GTGTTAAAAGACAAGCTGCATCTAGAAATCCAATTAGAGCTAGACAAGAAAGAGCTGCTC
AAGAAGAAGCAGATGCAAAGAAAAGATTGGAAGAACAAGCCAAAGCTAATGCATCATCAA
CAACAACAACTGTAACAGTTACAAATTCAAAACCAAATGTTCAATCAGCATTAGGTGGAT
TTGGATTATTATATTCAGTTGGAGCACCAGATGAAACAATGTTTAAAACAAGAGCTGAAA
GAATTCAATCAGCAATGGATAATTCATCATCATCAAATCCATTCTCATTAAATCANNNNN
NNNNN

>VHE526Z.Seq
NNNNNNNNNNTCAAAGAATATNTCATCGAAATCTTTAAATAGCAATGGTAGATNTAAATT
GGTTGAATTTGAAGAAGGTAAAGAATCTGAAGAATTCTTTAAACTTTTAGGTGGTCGTCA
ACCATATCCATTATCAAAATCCACTTCAAGAGTTGAACCACGTCTTTATCATTGTACTGT
TGGTTCTGGTGCTTTTGTTGTAGATGAAGTCACTTCTTTTGCTCAAGAGGATTTACTTCA
AGAGGATGTTTTCATCGTTGATGGTATCGAACAAATTTTCATTTGGATCGGTACTGAAAC
CACTGAAACTGAACGTAAATCTTCAATGGAAGTAGCAGTTGAATACTCTTCAACTTTACC
ATCACCAAGAAAACAAAATATTCCCGTCTATCTCACTTATCATGGTAAAGAACCTTACAT
TTTCACAAGCCTATTCCATGGTTGGGACTTTTCAAAGAGAGTCATTCCAACTATCTNTCT
TGATCAAGATTTACTTTTAGCAAAGGAAATATTAGAACTTTATACTAAAAAATATACATA
TGATCAATTAGTAAAGAAACAATATCCAAAAGGTATAGATGGTTCTCGTTTAGAAGAATA
TTTATCTGATGAAGAATTCATTTCAATTTTCAAGATAACTCTTGCTCAATTTAAAACTTT
ATCTTT

>VHE526P.Seq
AAAAACCACCAGTTGAACCAGTTGTAGTTCAAAAATCACCAGTTGTTCAACAACAACCAA
CAACATCAACTTATAAACATGTATTTAATCCAGTACAACAACAACAACAACAAACTACTC
AATCTACAACAGTTAAACAACCAGAAATACCAAAAGTTGTAGAAGAACCAAAGAAAGTAG
AACCAACACCAACACCAAAACCATTACCAAAACCAGAACCAACAATTGTTATTCCAACTC
AAGTTAAACAACCAGAAATACCAAAAGTTGTAGAAGAACCAAAGAAAGTAGAACAAGTTT
CAATTATTAAAACTGATGAAAATGTTCAAACATTAGCACCATTACCAGAGAAAGCTGAAG
GTGTTAAAAGACAAGCTGCATCTAGAAATCCAATTAGAGCTAGACAAGAAAGAGCTGCTC
AAGAAGAAGCAGATGCAAAGAAAAGATTGGAAGAACAAGCCAAAGCTAATGCATCATCAA
CAACAACAACTGTAACAGTTACAAATTCAAAACCAAATGTTCAATCAGCATTAGGTGGAT
TTGGATTATTATATTCAGTTGGAGCACCAGATGAAACAATGTTTAAAACAAGAGCTGAAA
GAATTCAATCAGCAATGGATAATTCATCATCATCAAATCCATTCTCATTAAATCA-----
-----TCAAAGAATATNTCATCGAAATCTTTAAATAGCAATGGTAGATNTAAATTGGTTG
AATTTGAAGAAGGTAAAGAATCTGAAGAATTCTTTAAACTTTTAGGTGGTCGTCAACCAT
ATCCATTATCAAAATCCACTTCAAGAGTTGAACCACGTCTTTATCATTGTACTGTTGGTT
CTGGTGCTTTTGTTGTAGATGAAGTCACTTCTTTTGCTCAAGAGGATTTACTTCAAGAGG
ATGTTTTCATCGTTGATGGTATCGAACAAATTTTCATTTGGATCGGTACTGAAACCACTG
AAACTGAACGTAAATCTTCAATGGAAGTAGCAGTTGAATACTCTTCAACTTTACCATCAC
CAAGAAAACAAAATATTCCCGTCTATCTCACTTATCATGGTAAAGAACCTTACATTTTCA
CAAGCCTATTCCATGGTTGGGACTTTTCAAAGAGAGTCATTCCAACTATCTNTCTTGATC
AAGATTTACTTTTAGCAAAGGAAATATTAGAACTTTATACTAAAAAATATACATATGATC
AATTAGTAAAGAAACAATATCCAAAAGGTATAGATGGTTCTCGTTTAGAAGAATATTTAT
CTGATGAAGAATTCATTTCAATTTTCAAGATAACTCTTGCTCAATTTAAAACTTTATCTT
T