>VHE516 (VHE516Q) /CSM/VH/VHE5-A/VHE516Q.Seq.d/
XXXXXXXXXXTCACATTTACCAGTAGTTTACATTTTCACTCATGACTCTGTTGGTGTTGG
TGAAGATGGTCCAACTCATCAACCAGTTGAAACCGTTTCAGGTCTTCGTATGATTCCAAA
CTTGGATGTCATTCGTCCAGCCGATCCAGAAGAAACAGCTGCTGCTTTATCTTTGGCTTA
TGNTCGTGCCGATGGTCCAACCCTTTTATCATTAACTCGTCAAAATCTTCCATTCNTCCC
AGGTACCGCTCAAAAGAAGAGAGAAGGTANTNTCAGAGGTGGTTACATTGTCGTTAGTGA
AACCGCTCCATTAAGAATGATCTTAATTGCCACTGGTTCAGAAGTTCAACATTGTGTTGA
AGCCGCTAAATTATTAGGTGATGATATTCGTGTCGTCTCTATGCCATGCACTGAACTTTT
CGATCGTCAATCAAATGAATACAAACAATCCGTACTTCCAAGTGGTTGTAGAAATCGTAT
CGCTATGGAAGCTGGTGTCACTTCANTCTGGTATAAATATGTTGGTCTTGATGGTAAAGT
CATTGGTATTGATAGATTTGGTATGTCTGCACCAGGTAATGCCGTTATGAAACAATTAGG
TATGACTTCTGAAAATTTAGTAAACATTTCAAAACAATAAAAAT

---HLPVVYIFTHDSVGVGEDGPTHQPVETVSGLRMIPNLDVIRPADPEETAAALSLAYX
RADGPTLLSLTRQNLPFXPGTAQKKREGXXRGGYIVVSETAPLRMILIATGSEVQHCVEA
AKLLGDDIRVVSMPCTELFDRQSNEYKQSVLPSGCRNRIAMEAGVTSXWYKYVGLDGKVI
GIDRFGMSAPGNAVMKQLGMTSENLVNISKQ*k

Frame A:
---hiyq*ftfslmtllvlvkmvqlinqlkpfqvfv*fqtwmsfvqpiqkkqlllylwlm
xvpmvqpfyh*lvkifhssqvplkrrekvxsevvtlslvkplh*e*s*lplvqkfnivlk
plny*vmifvsslchalnfsivnqmntnnpyfqvvveivslwklvslxsginmlvlmvks
lvlidlvclhqvmpl*nn*v*llki**tfqnnkn


Frame B:
---tftsslhfhs*lcwcw*rwsnssts*nrfrssydsklgchsssrsrrnsccfifglx
scrwsnpfiinsskssixpryrskeerrxxqrwlhcr**nrsikndlnchwfrsstlc*s
r*iir**yscrlyamh*tfrssik*iqtirtskwl*ksyrygswchfxlv*icws*w*sh
wy**iwyvctr*cryetirydf*kfskhfktik


Frame C:
---HLPVVYIFTHDSVGVGEDGPTHQPVETVSGLRMIPNLDVIRPADPEETAAALSLAYX
RADGPTLLSLTRQNLPFXPGTAQKKREGXXRGGYIVVSETAPLRMILIATGSEVQHCVEA
AKLLGDDIRVVSMPCTELFDRQSNEYKQSVLPSGCRNRIAMEAGVTSXWYKYVGLDGKVI
GIDRFGMSAPGNAVMKQLGMTSENLVNISKQ*k

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE516 (VHE516Q) /CSM/VH/VHE5-A/VHE516Q.Seq.d/                       1227   0.0
VHP168 (VHP168Q) /CSM/VH/VHP1-C/VHP168Q.Seq.d/                       1219   0.0
VHN785 (VHN785Q) /CSM/VH/VHN7-D/VHN785Q.Seq.d/                       1219   0.0
VHK769 (VHK769Q) /CSM/VH/VHK7-C/VHK769Q.Seq.d/                       1219   0.0
VHH745 (VHH745Q) /CSM/VH/VHH7-B/VHH745Q.Seq.d/                       1219   0.0
VHE496 (VHE496Q) /CSM/VH/VHE4-D/VHE496Q.Seq.d/                       1219   0.0
VHB679 (VHB679Q) /CSM/VH/VHB6-D/VHB679Q.Seq.d/                       1219   0.0
VFF246 (VFF246Q) /CSM/VF/VFF2-B/VFF246Q.Seq.d/                       1219   0.0
VFD821 (VFD821Q) /CSM/VF/VFD8-A/VFD821Q.Seq.d/                       1219   0.0
SFJ605 (SFJ605Q) /CSM/SF/SFJ6-A/SFJ605Q.Seq.d/                       1219   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.  1219   0.0    1
EF216683|EF216683.1 Karlodinium micrum transketolase mRNA, partial cds.    58   5e-12  3
ER462265|ER462265.1 1092963900917 Global-Ocean-Sampling_GS-35-01-01-1P5KB marine metagenome genomic clone 1061002399063 5', genomic survey sequence.    84   6e-12  1
BQ540728|BQ540728.1 PTAM1055 Phaeodactylum tricornutum Uni-Zap XR Phaeodactylum tricornutum cDNA 5', mRNA sequence.    68   2e-11  2
AE006393|AE006393.1 Lactococcus lactis subsp. lactis IL1403 section 155 of 218 of the complete genome.    42   5e-11  5
CD384397|CD384397.1 PTMM09570 Phaeodactylum tricornutum Uni-Zap XR Phaeodactylum tricornutum cDNA 5', mRNA sequence.    68   4e-10  2
CD379071|CD379071.1 PTMM04240 Phaeodactylum tricornutum Uni-Zap XR Phaeodactylum tricornutum cDNA 5', mRNA sequence.    68   4e-10  2
EK303511|EK303511.1 1095462372648 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061005791520 5', genomic survey sequence.    76   1e-09  1
EK248242|EK248242.1 1095460267104 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061005791520 3', genomic survey sequence.    76   1e-09  1
EK303511|EK303511.1 1095462372648 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061005791520 5', genomic survey sequence.    76   1e-09  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q556J0) RecName: Full=Transketolase;          Short=TK;        ...   405   e-112
AM746676_3991(AM746676|pid:none) Sorangium cellulosum 'So ce 56'...   267   2e-70
CP001032_3788(CP001032|pid:none) Opitutus terrae PB90-1, complet...   266   3e-70
(A6YB01) RecName: Full=Transketolase;          Short=TK;        ...   262   7e-69
EF216683_1(EF216683|pid:none) Karlodinium micrum transketolase m...   260   3e-68
AJ821796_1(AJ821796|pid:none) Guillardia theta partial mRNA for ...   257   2e-67
EF216685_1(EF216685|pid:none) Bigelowiella natans transketolase ...   256   5e-67
EF216682_1(EF216682|pid:none) Karlodinium micrum transketolase m...   255   9e-67
EF216681_1(EF216681|pid:none) Isochrysis galbana putative plasti...   219   4e-56
AP009484_999(AP009484|pid:none) Macrococcus caseolyticus JCSC540...   219   4e-56

 psg: 0.75  gvh: 0.54  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.14  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: peroxisomal

>> prediction for VHE516 is cyt

>VHE516Z.Seq
NNNNNNNNNNTCACATTTACCAGTAGTTTACATTTTCACTCATGACTCTGTTGGTGTTGG
TGAAGATGGTCCAACTCATCAACCAGTTGAAACCGTTTCAGGTCTTCGTATGATTCCAAA
CTTGGATGTCATTCGTCCAGCCGATCCAGAAGAAACAGCTGCTGCTTTATCTTTGGCTTA
TGNTCGTGCCGATGGTCCAACCCTTTTATCATTAACTCGTCAAAATCTTCCATTCNTCCC
AGGTACCGCTCAAAAGAAGAGAGAAGGTANTNTCAGAGGTGGTTACATTGTCGTTAGTGA
AACCGCTCCATTAAGAATGATCTTAATTGCCACTGGTTCAGAAGTTCAACATTGTGTTGA
AGCCGCTAAATTATTAGGTGATGATATTCGTGTCGTCTCTATGCCATGCACTGAACTTTT
CGATCGTCAATCAAATGAATACAAACAATCCGTACTTCCAAGTGGTTGTAGAAATCGTAT
CGCTATGGAAGCTGGTGTCACTTCANTCTGGTATAAATATGTTGGTCTTGATGGTAAAGT
CATTGGTATTGATAGATTTGGTATGTCTGCACCAGGTAATGCCGTTATGAAACAATTAGG
TATGACTTCTGAAAATTTAGTAAACATTTCAAAACAATAAAAAT