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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE513
Library VH
(Link to library)
Clone ID VHE513
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13028-1
Original site URL
Representative seq. ID VHE513P
(Link to Original site)
Representative DNA sequence 
>VHE513 (VHE513Q) /CSM/VH/VHE5-A/VHE513Q.Seq.d/
AGCAAATCCAAAAGAATGACAACTACTAATTGGAGTTTAAAAGTTGATAGAGGCAGACAA
ACTTGGGAGTACTCTCAAGAGAAAAAAGAAGCAACAGATGTTGATATTCATTTATTAAGA
TTAAAAGAACCAGGAACACATTGTCCAGAAGGATGTGATTTAAATAGAGCAAAAACACCA
CAACAAGCAATTAAAAAAGCATTCCAATATTTCTCAAAAGTTCAAACTGAGGATGGTCAT
TGGGCAGGTGATTATGGTGGTCCAATGTTTTTATTACCAGGTTTAGTCATTACATGTTAT
GTAACAGGTTATCAATTACCAGAATCAACTCAAAGAGAAATCATTAGATATTTATTCAAT
AGACAAAATCCAGTTGATGGTGGTTGGGGTCTTCACATTGAGGCACATTCAGATATTTTT
GGAACAACATTACAATATGTTTCATTAAGATTATTAGGAGTACCAGCAGATCATCCATCA
GTAGTAAAAGCAAGAACATTTTTATTACAAAATGGTGGTGCAACAGGTATTCCATCATGG
GGTAAATTTTGGTTAGCAACATTAAATGCTTATGATTGGAATGGTTTXXXXXXXXXXTCT
GCTAAATATCCAGATAGAGAATGCATTGAAAGAGGTATTAAATTCTTCATACAAAGACAA
TATCCNAATGGTGATTTCCCACAAGAATCTATTATTGGTGTTTTCAATTTCAATTGTATG
ATCAGTTATTCAAATTATAAAAATATTTTCCCATTATGGGCTTTATCTAGATATAATCAA
TTATATTTAAAATCTAAAATTTAAATATTTCTAA
sequence update 2002.10.25
Translated Amino Acid sequence 
SKSKRMTTTNWSLKVDRGRQTWEYSQEKKEATDVDIHLLRLKEPGTHCPEGCDLNRAKTP
QQAIKKAFQYFSKVQTEDGHWAGDYGGPMFLLPGLVITCYVTGYQLPESTQREIIRYLFN
RQNPVDGGWGLHIEAHSDIFGTTLQYVSLRLLGVPADHPSVVKARTFLLQNGGATGIPSW
GKFWLATLNAYDWNG---

---SAKYPDRECIERGIKFFIQRQYPNGDFPQESIIGVFNFNCMISYSNYKNIFPLWALS
RYNQLYLKSKI*ifl
Translated Amino Acid sequence (All Frames) 
Frame A:
SKSKRMTTTNWSLKVDRGRQTWEYSQEKKEATDVDIHLLRLKEPGTHCPEGCDLNRAKTP
QQAIKKAFQYFSKVQTEDGHWAGDYGGPMFLLPGLVITCYVTGYQLPESTQREIIRYLFN
RQNPVDGGWGLHIEAHSDIFGTTLQYVSLRLLGVPADHPSVVKARTFLLQNGGATGIPSW
GKFWLATLNAYDWNG---

---SAKYPDRECIERGIKFFIQRQYPNGDFPQESIIGVFNFNCMISYSNYKNIFPLWALS
RYNQLYLKSKI*ifl

Frame B:
anpke*qlligv*klieadklgstlkrkkkqqmlifiy*d*knqehivqkdvi*ieqkhh
nkqlkkhsnisqkfklrmvigqvimvvqcfyyqv*slhvm*qvinyqnqlkeksldiysi
dkiqlmvvgvftlrhiqifleqhynmfh*dy*eyqqiihq**kqehfyykmvvqqvfhhg
vnfg*qh*mlmigmv---

---llniqienalkevlnssykdnixmvishknlllvfsisiv*sviqiikifshyglyl
diinyi*nlkfkyf*

Frame C:
qiqkndny*lefks**rqtnlgvlsrekrsnrc*ysfikikrtrntlsrrm*fk*skntt
tsn*ksipiflkssn*gwslgr*lwwsnvfitrfshymlcnrlsitrinskrnh*ifiq*
tkss*wwlgssh*gtfryfwnniticfikiirstsrssissksknifitkwwcnrysimg
*ilvsnikcl*lewf---

---c*isr*rmh*kry*ilhtktisxw*fptriyywcfqfqlydqlfkl*kyfpimgfi*
i*siifki*nlnis
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ353 (VHQ353Q) /CSM/VH/VHQ3-C/VHQ353Q.Seq.d/                       1164   0.0
VHQ132 (VHQ132Q) /CSM/VH/VHQ1-B/VHQ132Q.Seq.d/                       1164   0.0
VHP346 (VHP346Q) /CSM/VH/VHP3-B/VHP346Q.Seq.d/                       1164   0.0
VHM690 (VHM690Q) /CSM/VH/VHM6-D/VHM690Q.Seq.d/                       1164   0.0
VHM553 (VHM553Q) /CSM/VH/VHM5-C/VHM553Q.Seq.d/                       1164   0.0
VHL721 (VHL721Q) /CSM/VH/VHL7-A/VHL721Q.Seq.d/                       1164   0.0
VHL445 (VHL445Q) /CSM/VH/VHL4-B/VHL445Q.Seq.d/                       1164   0.0
VHK502 (VHK502Q) /CSM/VH/VHK5-A/VHK502Q.Seq.d/                       1164   0.0
VHK355 (VHK355Q) /CSM/VH/VHK3-C/VHK355Q.Seq.d/                       1164   0.0
VHJ519 (VHJ519Q) /CSM/VH/VHJ5-A/VHJ519Q.Seq.d/                       1164   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF159949|AF159949.1 Dictyostelium discoideum cycloartenol synthase (CAS1) mRNA, partial cds.  1132   0.0    1
EL577379|EL577379.1 Physarum08327 Physarum polycephalum starvation stress library Physarum polycephalum cDNA clone PpolyN1d98h02.t7, mRNA sequence.    70   1e-07  1
EL577097|EL577097.1 Physarum08344 Physarum polycephalum starvation stress library Physarum polycephalum cDNA clone PpolyN1d95e10.t7, mRNA sequence.    70   1e-07  1
EL576522|EL576522.1 Physarum08350 Physarum polycephalum starvation stress library Physarum polycephalum cDNA clone PpolyN1d88e08.t7, mRNA sequence.    70   1e-07  1
EL576351|EL576351.1 Physarum08329 Physarum polycephalum starvation stress library Physarum polycephalum cDNA clone PpolyN1d86g02.t7, mRNA sequence.    70   1e-07  1
EL575965|EL575965.1 Physarum08345 Physarum polycephalum starvation stress library Physarum polycephalum cDNA clone PpolyN1d82d02.t7, mRNA sequence.    70   1e-07  1
EL575877|EL575877.1 Physarum08369 Physarum polycephalum starvation stress library Physarum polycephalum cDNA clone PpolyN1d81c11.t7, mRNA sequence.    70   1e-07  1
EL575306|EL575306.1 Physarum08366 Physarum polycephalum starvation stress library Physarum polycephalum cDNA clone PpolyN1d75c05.t7, mRNA sequence.    70   1e-07  1
EL574807|EL574807.1 Physarum08339 Physarum polycephalum starvation stress library Physarum polycephalum cDNA clone PpolyN1d69f02.t7, mRNA sequence.    70   1e-07  1
EL574777|EL574777.1 Physarum08333 Physarum polycephalum starvation stress library Physarum polycephalum cDNA clone PpolyN1d69c04.t7, mRNA sequence.    70   1e-07  1
dna update 2007. 8.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55D85) RecName: Full=Cycloartenol synthase;          EC=5.4.99...   407   e-112
(P84466) RecName: Full=Lanosterol synthase;          EC=5.4.99.7...   196   1e-48
AK044016_1(AK044016|pid:none) Mus musculus 10 days neonate corte...   191   2e-47
AK312489_1(AK312489|pid:none) Homo sapiens cDNA, FLJ92849, highl...   189   7e-47
BC035638_1(BC035638|pid:none) Homo sapiens lanosterol synthase (...   189   7e-47
AJ239031_1(AJ239031|pid:none) Homo sapiens LSS gene, partial, ex...   189   7e-47
(P48450) RecName: Full=Lanosterol synthase;          EC=5.4.99.7...   189   1e-46
GN121451_1(GN121451|pid:none) Sequence 4347 from Patent WO200903...   187   5e-46
EU665687_1(EU665687|pid:none) Hypholoma sublateritium oxidosqual...   186   6e-46
BX255930_10(BX255930|pid:none) Zebrafish DNA sequence from clone...   186   6e-46
protein update 2009. 6.30
PSORT 

 psg: 0.83  gvh: 0.44  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.40  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.30  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: mitochondrial
	 28.0 %: cytoplasmic
	 20.0 %: nuclear
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for VHE513 is mit
5' end seq. ID VHE513F
5' end seq. 
>VHE513F.Seq
AGCAAATCCAAAAGAATGACAACTACTAATTGGAGTTTAAAAGTTGATAGAGGCAGACAA
ACTTGGGAGTACTCTCAAGAGAAAAAAGAAGCAACAGATGTTGATATTCATTTATTAAGA
TTAAAAGAACCAGGAACACATTGTCCAGAAGGATGTGATTTAAATAGAGCAAAAACACCA
CAACAAGCAATTAAAAAAGCATTCCAATATTTCTCAAAAGTTCAAACTGAGGATGGTCAT
TGGGCAGGTGATTATGGTGGTCCAATGTTTTTATTACCAGGTTTAGTCATTACATGTTAT
GTAACAGGTTATCAATTACCAGAATCAACTCAAAGAGAAATCATTAGATATTTATTCAAT
AGACAAAATCCAGTTGATGGTGGTTGGGGTCTTCACATTGAGGCACATTCAGATATTTTT
GGAACAACATTACAATATGTTTCATTAAGATTATTAGGAGTACCAGCAGATCATCCATCA
GTAGTAAAAGCAAGAACATTTTTATTACAAAATGGTGGTGCAACAGGTATTCCATCATGG
GGTAAATTTTGGTTAGCAACATTAAATGCTTATGATTGGAATGGTTTNNNNNNNNNN
Length of 5' end seq. 597
3' end seq. ID VHE513Z
3' end seq. 
>VHE513Z.Seq
NNNNNNNNNNTCTGCTAAATATCCAGATAGAGAATGCATTGAAAGAGGTATTAAATTCTT
CATACAAAGACAATATCCNAATGGTGATTTCCCACAAGAATCTATTATTGGTGTTTTCAA
TTTCAATTGTATGATCAGTTATTCAAATTATAAAAATATTTTCCCATTATGGGCTTTATC
TAGATATAATCAATTATATTTAAAATCTAAAATTTAAATATTTCTAA
Length of 3' end seq. 227
Connected seq. ID VHE513P
Connected seq. 
>VHE513P.Seq
AGCAAATCCAAAAGAATGACAACTACTAATTGGAGTTTAAAAGTTGATAGAGGCAGACAA
ACTTGGGAGTACTCTCAAGAGAAAAAAGAAGCAACAGATGTTGATATTCATTTATTAAGA
TTAAAAGAACCAGGAACACATTGTCCAGAAGGATGTGATTTAAATAGAGCAAAAACACCA
CAACAAGCAATTAAAAAAGCATTCCAATATTTCTCAAAAGTTCAAACTGAGGATGGTCAT
TGGGCAGGTGATTATGGTGGTCCAATGTTTTTATTACCAGGTTTAGTCATTACATGTTAT
GTAACAGGTTATCAATTACCAGAATCAACTCAAAGAGAAATCATTAGATATTTATTCAAT
AGACAAAATCCAGTTGATGGTGGTTGGGGTCTTCACATTGAGGCACATTCAGATATTTTT
GGAACAACATTACAATATGTTTCATTAAGATTATTAGGAGTACCAGCAGATCATCCATCA
GTAGTAAAAGCAAGAACATTTTTATTACAAAATGGTGGTGCAACAGGTATTCCATCATGG
GGTAAATTTTGGTTAGCAACATTAAATGCTTATGATTGGAATGGTTT----------TCT
GCTAAATATCCAGATAGAGAATGCATTGAAAGAGGTATTAAATTCTTCATACAAAGACAA
TATCCNAATGGTGATTTCCCACAAGAATCTATTATTGGTGTTTTCAATTTCAATTGTATG
ATCAGTTATTCAAATTATAAAAATATTTTCCCATTATGGGCTTTATCTAGATATAATCAA
TTATATTTAAAATCTAAAATTTAAATATTTCTAA
Length of connected seq. 804
Full length Seq ID -
Full length Seq. -
Length of full length seq. -