>VHE487 (VHE487Q) /CSM/VH/VHE4-D/VHE487Q.Seq.d/
XXXXXXXXXXTTATCATCTTCATCCCAAAAGAAGAAAAAAGAAGTTAGTAGAAGTGATGA
TGATGACGATGATGAAAAAATAGAAAATTGTGTTGAAAGTTATGAAAGTGACGGAGGTGA
TCAATCAAGTGAACAAGATGAAGAAGAAGAAGACGATTGTGATGATTTCCATGAAGATCT
AGACGAAGATTTAGGTGCTACCGCAATGGATGTTGATAAAAAAGAATGTGAAAAAGAATG
TAAAAAGAAAGATTNTTCAAAAGTTGACCTTAAAGTCAAACCTTCAAAAGTACCTNTACC
ATCAAGATCACCTTCAGTTTCAAAACCAACAACAACATCACTACTTTNTCCATNTCCAAA
ATCAGCACCATCAGCACCATCACAACAAAAATCAGTTAAAAGTACTGGTGATTTATTAAT
TGATTTATTAAAAATTCAAAAATCAAATGGTTNTTGGACAAAATCTTCTATTGATCAATT
GAAAATCCCAACTGACAAAGCACCTGCCGAATTATCAACTACTGAATTAAATGACATTTG
GGTAACCATTATCGTTATTGCAAAGATTGTAAAATTNTTNTCATNTGAAAA

---SSSSQKKKKEVSRSDDDDDDEKIENCVESYESDGGDQSSEQDEEEEDDCDDFHEDLD
EDLGATAMDVDKKECEKECKKKDXSKVDLKVKPSKVPXPSRSPSVSKPTTTSLLXPXPKS
APSAPSQQKSVKSTGDLLIDLLKIQKSNGXWTKSSIDQLKIPTDKAPAELSTTELNDIWV
TIIVIAKIVKXXSXE

Frame A:
---yhlhpkrrkkklvevmmmtmmkk*kivlkvmkvtevinqvnkmkkkktivmismki*
tki*vlpqwmlikknvkknvkrkixqkltlksnlqkylyhqdhlqfqnqqqhhyfxhxqn
qhhqhhhnknqlkvlviy*liy*kfknqmvxgqnlllin*ksqltkhlpnyqlln*mtfg
*plsllqrl*nxxhxk


Frame B:
---iifipkeekrs**k****r**knrklc*kl*k*rr*sik*tr*rrrrrl**fp*rsr
rrfrcyrngc**krm*krm*kerxfks*p*sqtfkstxtikitfsfktnnnittxsxski
stistittkis*kyw*fin*fiknskikwxldkify*sienpn*qstcriiny*ik*hlg
nhyryckdckixxx*k


Frame C:
---SSSSQKKKKEVSRSDDDDDDEKIENCVESYESDGGDQSSEQDEEEEDDCDDFHEDLD
EDLGATAMDVDKKECEKECKKKDXSKVDLKVKPSKVPXPSRSPSVSKPTTTSLLXPXPKS
APSAPSQQKSVKSTGDLLIDLLKIQKSNGXWTKSSIDQLKIPTDKAPAELSTTELNDIWV
TIIVIAKIVKXXSXE

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI519 (VSI519Q) /CSM/VS/VSI5-A/VSI519Q.Seq.d/                        626   e-179
VHE487 (VHE487Q) /CSM/VH/VHE4-D/VHE487Q.Seq.d/                        626   e-179
SSC591 (SSC591Q) /CSM/SS/SSC5-D/SSC591Q.Seq.d/                        626   e-179
FC-BM11 (FC-BM11Q) /CSM/FC/FC-BM/FC-BM11Q.Seq.d/                      597   e-170
CHM152 (CHM152Q) /CSM/CH/CHM1-C/CHM152Q.Seq.d/                        571   e-162
VHG126 (VHG126Q) /CSM/VH/VHG1-B/VHG126Q.Seq.d/                        190   2e-47
SSL591 (SSL591Q) /CSM/SS/SSL5-D/SSL591Q.Seq.d/                        186   3e-46
SHE422 (SHE422Q) /CSM/SH/SHE4-A/SHE422Q.Seq.d/                        131   1e-29
CHK643 (CHK643Q) /CSM/CH/CHK6-B/CHK643Q.Seq.d/                        117   2e-25
SHD654 (SHD654Q) /CSM/SH/SHD6-C/SHD654Q.Seq.d/                        105   8e-22

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

EH748504|EH748504.1 CNMS7844.b1_H17.ab1 CNM(LMS) spotted knapweed Centaurea maculosacDNA clone CNMS7844, mRNA sequence.    38   0.016  2
EH740364|EH740364.1 CNMS13648.b1_O04.ab1 CNM(LMS) spotted knapweed Centaurea maculosa cDNA clone CNMS13648, mRNA sequence.    38   0.016  2
DW507182|DW507182.1 GH_TMIRS_111_G10_F Cotton Normalized Library dT primed Gossypium hirsutum cDNA, mRNA sequence.    52   0.019  1
DW502678|DW502678.1 GH_TMIRS_068_F02_F Cotton Normalized Library dT primed Gossypium hirsutum cDNA, mRNA sequence.    52   0.019  1
DR453290|DR453290.1 CM011F06 Cotton Lambda Zap Express Library Gossypium hirsutum cDNA, mRNA sequence.    52   0.019  1
DN804645|DN804645.1 G.hir-8-10 DAA bolls irrigated 445 8-10 DAA cotton bolls from irrigated plants Gossypium hirsutum cDNA, mRNA sequence.    52   0.019  1
DN779312|DN779312.1 CFUS3.4C05 Cotton Root and Hypocotyl tissues infected with Fusarium oxysporum f.sp. vasinfectum (Fov) Lambda ZIPLOX Library (CFUS) Gossypium hirsutum cDNA clone CFUS3.4C05 5' similar to Unknown Protein, mRNA sequence.    52   0.019  1
CO495456|CO495456.1 G.h.fbr-sw04846 G.h.fbr-sw Gossypium hirsutum cDNA, mRNA sequence.    52   0.019  1
BG445214|BG445214.1 GA__Ea0027E04f Gossypium arboreum 7-10 dpa fiber library Gossypium arboreum cDNA clone GA__Ea0027E04f, mRNA sequence.    52   0.019  1
BE052814|BE052814.2 GA__Ea0033G19f Gossypium arboreum 7-10 dpa fiber library Gossypium arboreum cDNA clone GA__Ea0033G19f, mRNA sequence.    52   0.019  1

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 417,582,858

 psg: 0.28  gvh: 0.17  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: cytoskeletal

>> prediction for VHE487 is nuc

>VHE487Z.Seq
NNNNNNNNNNTTATCATCTTCATCCCAAAAGAAGAAAAAAGAAGTTAGTAGAAGTGATGA
TGATGACGATGATGAAAAAATAGAAAATTGTGTTGAAAGTTATGAAAGTGACGGAGGTGA
TCAATCAAGTGAACAAGATGAAGAAGAAGAAGACGATTGTGATGATTTCCATGAAGATCT
AGACGAAGATTTAGGTGCTACCGCAATGGATGTTGATAAAAAAGAATGTGAAAAAGAATG
TAAAAAGAAAGATTNTTCAAAAGTTGACCTTAAAGTCAAACCTTCAAAAGTACCTNTACC
ATCAAGATCACCTTCAGTTTCAAAACCAACAACAACATCACTACTTTNTCCATNTCCAAA
ATCAGCACCATCAGCACCATCACAACAAAAATCAGTTAAAAGTACTGGTGATTTATTAAT
TGATTTATTAAAAATTCAAAAATCAAATGGTTNTTGGACAAAATCTTCTATTGATCAATT
GAAAATCCCAACTGACAAAGCACCTGCCGAATTATCAACTACTGAATTAAATGACATTTG
GGTAACCATTATCGTTATTGCAAAGATTGTAAAATTNTTNTCATNTGAAAA