>VHE468 (VHE468Q) /CSM/VH/VHE4-C/VHE468Q.Seq.d/
ATTTAATTTTTTTAATTAATCATAGTCAACAAAATGTGTAAATATAATGATCATAAATAT
TTAGTAGAAAAGGTAGATAGATTGGTATTACCAGAAAATGTTGTACCAATTAAATATGAT
TTACATTTAAAACCAAATTTAAAAGAATTTACTTTTAAAGGTGAAGAGACCATCACAGTT
CAAGTAAAACAACCAACAAAAACCATAACTATACATTCAATTGAAATTGAAATTCAATCA
GCTTCAATAAAATCATCATCATCATCTCAATCATCAAAATCAATTACATTTTATGAACCA
GAAGAAGTTGTCATCTTTGAATTTGAAAATGAATTATCAGTTGGTGAATATTGTTTATCA
TTGGTATTTACTGGTTTATTAAATGACAAATTAAAAGGTTTCTATCGTTCAAAGTATACT
GTTAAAGGTGAAGATCGTTATTTAGCAACAACTCAATTCGAAGCCACCGATGCTCGTCGT
TCTTTTCCATGTTTTGATGAACCAGCTCATAAAGCAGTTTTCAATATCACTTTAACTGTC
TCTGAATGTCATACCGCTXXXXXXXXXXTTGAAGTTAAATCAGGTGAATCATCNTCTGAC
ACTTTGTTAAGAAATAAAGTAAACTCTTATTTGGGTATTTTGGGTGACAAAGAGATTGTG
GCCGAGGCTAGAAAGAGATTTGAGCAATTCAAAGTCGACCAATCTTCTTTACCTTCCGAT
ATCCGTTCAAGTGTTTTAGTCACTGTTGTAAAGAATGGTTCCGAAGCTGAACAACAAGAA
ATTATCAATAGATACCTCGCTTCCAATGATATTGCAGAGAAATCAAGTTTATTGTCAGTT
GTTTGCAAATCACCATCCAGTGCATTGGTATTGAAGGCTTTGGAATTCTCTGTCTCAAAA
GATGTTAGAACTTGTGAATCCTATATGCTTTGGAGAGTCGGAAATGAATTTAAACCAGTA
GTTTGGAAATATTTCACTGAAAACTTTAAATCAATCAATGAAACCTTTAATCAAAATGTT
TTATTCGCTTATATGATCTCTTTTGCTTTGTCCTCAAAAATGACTGATCAACAATTACAA
CAAGTTGAAGATTTCTTCAAACAAAATCCAGTTGCAATTGCTGATCGTTCAATTAAACAA
GATTTAGAACAAATTAGAAATAATACAAAATGGTTTAATTCATTTAATAAAGATTTATTA
AATTGGATTAAAAAATAAATAATCTAATTTAATAAAGATTTATAAATTTGGATTTTTATA
AAAAATAAAATTTATTTAATTTTTATTATTTTTTTATTAAGTTTTATAAATAATAAAATA
AATTTTCAAATTAATAAA

i*ff*LIIVNKMCKYNDHKYLVEKVDRLVLPENVVPIKYDLHLKPNLKEFTFKGEETITV
QVKQPTKTITIHSIEIEIQSASIKSSSSSQSSKSITFYEPEEVVIFEFENELSVGEYCLS
LVFTGLLNDKLKGFYRSKYTVKGEDRYLATTQFEATDARRSFPCFDEPAHKAVFNITLTV
SECHTA---

---EVKSGESSSDTLLRNKVNSYLGILGDKEIVAEARKRFEQFKVDQSSLPSDIRSSVLV
TVVKNGSEAEQQEIINRYLASNDIAEKSSLLSVVCKSPSSALVLKALEFSVSKDVRTCES
YMLWRVGNEFKPVVWKYFTENFKSINETFNQNVLFAYMISFALSSKMTDQQLQQVEDFFK
QNPVAIADRSIKQDLEQIRNNTKWFNSFNKDLLNWIKK*ii*fnkdl*iwifiknkiyli
fiifllsfinnkinfqink

Frame A:
i*ff*LIIVNKMCKYNDHKYLVEKVDRLVLPENVVPIKYDLHLKPNLKEFTFKGEETITV
QVKQPTKTITIHSIEIEIQSASIKSSSSSQSSKSITFYEPEEVVIFEFENELSVGEYCLS
LVFTGLLNDKLKGFYRSKYTVKGEDRYLATTQFEATDARRSFPCFDEPAHKAVFNITLTV
SECHTA---

---lklnqvnhxltlc*eik*tliwvfwvtkrlwprlerdlsnskstnllylpisvqvf*
sll*rmvpklnnkklsidtslpmilqrnqvycqlfanhhpvhwy*rlwnslsqkmlelvn
picfgesemnlnq*fgnislktlnqsmkplikmfysli*slllcpqk*linnynklkiss
nkiqlqllivqlnki*nkleiiqnglihlikiy*iglknk*snlikiykfgfl*kikfi*
fllffy*vl*iik*ifkli

Frame B:
fnffn*s*stkcvnimiini**kr*idwyyqkmlyqlnmiyi*nqi*knlllkvkrpsqf
k*nnqqkp*lyiqlklkfnqlq*nhhhhlnhqnqlhfmnqkklsslnlkmnyqlvnivyh
wyllvy*mtn*kvsivqsillkvkivi*qqlnskppmlvvlfhvlmnqlikqfsisl*ls
lnvip---

---*s*ir*iix*hfvkk*skllfgyfg*qrdcgrg*kei*aiqsrpifftfrypfkcfs
hcckewfrs*ttrnyq*iprfq*ycreikfivsclqitiqcigiegfgilclkrc*nl*i
lyalesrk*i*tssleifh*kl*inq*nl*skcfirlydlfcfvlknd*stitts*rflq
tksscnc*sfn*trfrtn*k*ykmv*fi**rfikld*kinnli**rfinldfykk*nlfn
fyyffikfyk**nkfsn**

Frame C:
liflinhsqqnv*i**s*ifsrkgr*igitrkcctn*i*ftfktkfkriyf*r*rdhhss
skttnknhnytfn*n*nsisfnkiiiiisiikinyil*trrschl*i*k*iisw*ilfii
giywfik*qikrflsfkvyc*r*rslfsnnsirshrcssffsmf**tss*ssfqyhfncl
*msyr---

---EVKSGESSSDTLLRNKVNSYLGILGDKEIVAEARKRFEQFKVDQSSLPSDIRSSVLV
TVVKNGSEAEQQEIINRYLASNDIAEKSSLLSVVCKSPSSALVLKALEFSVSKDVRTCES
YMLWRVGNEFKPVVWKYFTENFKSINETFNQNVLFAYMISFALSSKMTDQQLQQVEDFFK
QNPVAIADRSIKQDLEQIRNNTKWFNSFNKDLLNWIKK*ii*fnkdl*iwifiknkiyli
fiifllsfinnkinfqink

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE468 (VHE468Q) /CSM/VH/VHE4-C/VHE468Q.Seq.d/                       1683   0.0
VHP741 (VHP741Q) /CSM/VH/VHP7-B/VHP741Q.Seq.d/                       1179   0.0
VHO863 (VHO863Q) /CSM/VH/VHO8-C/VHO863Q.Seq.d/                       1179   0.0
VHO805 (VHO805Q) /CSM/VH/VHO8-A/VHO805Q.Seq.d/                       1179   0.0
VHN658 (VHN658Q) /CSM/VH/VHN6-C/VHN658Q.Seq.d/                       1179   0.0
VHN267 (VHN267Q) /CSM/VH/VHN2-C/VHN267Q.Seq.d/                       1179   0.0
VHJ865 (VHJ865Q) /CSM/VH/VHJ8-C/VHJ865Q.Seq.d/                       1179   0.0
VHJ465 (VHJ465Q) /CSM/VH/VHJ4-C/VHJ465Q.Seq.d/                       1179   0.0
VHJ402 (VHJ402Q) /CSM/VH/VHJ4-A/VHJ402Q.Seq.d/                       1179   0.0
VHJ255 (VHJ255Q) /CSM/VH/VHJ2-C/VHJ255Q.Seq.d/                       1179   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY631393|AY631393.1 Dictyostelium discoideum puromycin-sensitive aminopeptidase-like protein (psa) mRNA, partial cds.   955   0.0    2
AC116330|AC116330.2 Dictyostelium discoideum chromosome 2 map 3191214-3323468 strain AX4, complete sequence.    42   0.066  7
CT352990|CT352990.1 Sus scrofa genomic clone CH242-458C8, genomic survey sequence.    44   0.22   2
AM285302|AM285302.1 Spiroplasma citri GII3-3X chromosome, contig Contig01B.    46   0.24   9
ER318883|ER318883.1 1092344163015 Global-Ocean-Sampling_GS-34-01-01-1P5-1P8KB marine metagenome genomic clone 1061001469974 3', genomic survey sequence.    36   0.39   3
DT174814|DT174814.1 JGI_ANNO44615.fwd ANNO Pimephales promelas Whole (M) Pimephales promelas cDNA clone ANNO44615 5', mRNA sequence.    48   0.70   1
AC153358|AC153358.2 Mus musculus chromosome 10 clone RP23-475N12, WORKING DRAFT SEQUENCE, 42 unordered pieces.    48   0.70   1
AC101118|AC101118.1 Mus musculus clone RP23-92N20, LOW-PASS SEQUENCE SAMPLING.    48   0.70   1
AC079501|AC079501.1 Mus musculus clone RP23-228N12, WORKING DRAFT SEQUENCE, 16 unordered pieces.    48   0.70   1
AC159266|AC159266.3 Mus musculus BAC clone RP24-64D24 from chromosome 8, complete sequence.    48   0.70   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY631393_1(AY631393|pid:none) Dictyostelium discoideum puromycin...   393   e-108
AF350242_1(AF350242|pid:none) Pichia angusta aminopeptidase gene...   134   7e-30
AE014296_282(AE014296|pid:none) Drosophila melanogaster chromoso...   133   1e-29
AE014296_281(AE014296|pid:none) Drosophila melanogaster chromoso...   133   1e-29
AF327435_1(AF327435|pid:none) Drosophila melanogaster puromycin-...   132   4e-29
(P55786) RecName: Full=Puromycin-sensitive aminopeptidase;      ...   122   4e-26
FM992688_411(FM992688|pid:none) Candida dubliniensis CD36 chromo...   121   7e-26
AP005572_11(AP005572|pid:none) Oryza sativa Japonica Group genom...   120   9e-26
BC098978_1(BC098978|pid:none) Xenopus laevis hypothetical protei...   120   1e-25
A65888_1(A65888|pid:none) Sequence 1 from Patent WO9738114.  &Y0...   120   1e-25

 psg: 0.53  gvh: 0.26  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VHE468 is cyt

>VHE468F.Seq
ATTTAATTTTTTTAATTAATCATAGTCAACAAAATGTGTAAATATAATGATCATAAATAT
TTAGTAGAAAAGGTAGATAGATTGGTATTACCAGAAAATGTTGTACCAATTAAATATGAT
TTACATTTAAAACCAAATTTAAAAGAATTTACTTTTAAAGGTGAAGAGACCATCACAGTT
CAAGTAAAACAACCAACAAAAACCATAACTATACATTCAATTGAAATTGAAATTCAATCA
GCTTCAATAAAATCATCATCATCATCTCAATCATCAAAATCAATTACATTTTATGAACCA
GAAGAAGTTGTCATCTTTGAATTTGAAAATGAATTATCAGTTGGTGAATATTGTTTATCA
TTGGTATTTACTGGTTTATTAAATGACAAATTAAAAGGTTTCTATCGTTCAAAGTATACT
GTTAAAGGTGAAGATCGTTATTTAGCAACAACTCAATTCGAAGCCACCGATGCTCGTCGT
TCTTTTCCATGTTTTGATGAACCAGCTCATAAAGCAGTTTTCAATATCACTTTAACTGTC
TCTGAATGTCATACCGCTNNNNNNNNNN

>VHE468Z.Seq
NNNNNNNNNNTTGAAGTTAAATCAGGTGAATCATCNTCTGACACTTTGTTAAGAAATAAA
GTAAACTCTTATTTGGGTATTTTGGGTGACAAAGAGATTGTGGCCGAGGCTAGAAAGAGA
TTTGAGCAATTCAAAGTCGACCAATCTTCTTTACCTTCCGATATCCGTTCAAGTGTTTTA
GTCACTGTTGTAAAGAATGGTTCCGAAGCTGAACAACAAGAAATTATCAATAGATACCTC
GCTTCCAATGATATTGCAGAGAAATCAAGTTTATTGTCAGTTGTTTGCAAATCACCATCC
AGTGCATTGGTATTGAAGGCTTTGGAATTCTCTGTCTCAAAAGATGTTAGAACTTGTGAA
TCCTATATGCTTTGGAGAGTCGGAAATGAATTTAAACCAGTAGTTTGGAAATATTTCACT
GAAAACTTTAAATCAATCAATGAAACCTTTAATCAAAATGTTTTATTCGCTTATATGATC
TCTTTTGCTTTGTCCTCAAAAATGACTGATCAACAATTACAACAAGTTGAAGATTTCTTC
AAACAAAATCCAGTTGCAATTGCTGATCGTTCAATTAAACAAGATTTAGAACAAATTAGA
AATAATACAAAATGGTTTAATTCATTTAATAAAGATTTATTAAATTGGATTAAAAAATAA
ATAATCTAATTTAATAAAGATTTATAAATTTGGATTTTTATAAAAAATAAAATTTATTTA
ATTTTTATTATTTTTTTATTAAGTTTTATAAATAATAAAATAAATTTTCAAATTAATAAA

>VHE468P.Seq
ATTTAATTTTTTTAATTAATCATAGTCAACAAAATGTGTAAATATAATGATCATAAATAT
TTAGTAGAAAAGGTAGATAGATTGGTATTACCAGAAAATGTTGTACCAATTAAATATGAT
TTACATTTAAAACCAAATTTAAAAGAATTTACTTTTAAAGGTGAAGAGACCATCACAGTT
CAAGTAAAACAACCAACAAAAACCATAACTATACATTCAATTGAAATTGAAATTCAATCA
GCTTCAATAAAATCATCATCATCATCTCAATCATCAAAATCAATTACATTTTATGAACCA
GAAGAAGTTGTCATCTTTGAATTTGAAAATGAATTATCAGTTGGTGAATATTGTTTATCA
TTGGTATTTACTGGTTTATTAAATGACAAATTAAAAGGTTTCTATCGTTCAAAGTATACT
GTTAAAGGTGAAGATCGTTATTTAGCAACAACTCAATTCGAAGCCACCGATGCTCGTCGT
TCTTTTCCATGTTTTGATGAACCAGCTCATAAAGCAGTTTTCAATATCACTTTAACTGTC
TCTGAATGTCATACCGCT----------TTGAAGTTAAATCAGGTGAATCATCNTCTGAC
ACTTTGTTAAGAAATAAAGTAAACTCTTATTTGGGTATTTTGGGTGACAAAGAGATTGTG
GCCGAGGCTAGAAAGAGATTTGAGCAATTCAAAGTCGACCAATCTTCTTTACCTTCCGAT
ATCCGTTCAAGTGTTTTAGTCACTGTTGTAAAGAATGGTTCCGAAGCTGAACAACAAGAA
ATTATCAATAGATACCTCGCTTCCAATGATATTGCAGAGAAATCAAGTTTATTGTCAGTT
GTTTGCAAATCACCATCCAGTGCATTGGTATTGAAGGCTTTGGAATTCTCTGTCTCAAAA
GATGTTAGAACTTGTGAATCCTATATGCTTTGGAGAGTCGGAAATGAATTTAAACCAGTA
GTTTGGAAATATTTCACTGAAAACTTTAAATCAATCAATGAAACCTTTAATCAAAATGTT
TTATTCGCTTATATGATCTCTTTTGCTTTGTCCTCAAAAATGACTGATCAACAATTACAA
CAAGTTGAAGATTTCTTCAAACAAAATCCAGTTGCAATTGCTGATCGTTCAATTAAACAA
GATTTAGAACAAATTAGAAATAATACAAAATGGTTTAATTCATTTAATAAAGATTTATTA
AATTGGATTAAAAAATAAATAATCTAATTTAATAAAGATTTATAAATTTGGATTTTTATA
AAAAATAAAATTTATTTAATTTTTATTATTTTTTTATTAAGTTTTATAAATAATAAAATA
AATTTTCAAATTAATAAA