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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFN716
Library AF
(Link to library)
Clone ID AFN716
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11229-1
Original site URL
Representative seq. ID AFN716P
(Link to Original site)
Representative DNA sequence 
>AFN716 (AFN716Q) /CSM/AF/AFN7-A/AFN716Q.Seq.d/
AATTAAAATCGATTCAATCGGNAGCAATTCATATCGTATCACTGCAATCTAAATTCACTC
AACCAAATACAACTCGTCCAAGGGATTTCAATGCAAACTCTATTTGGTGGATGCCAATTA
AAGTCATTGACAATTGTGAAAACACTGATGACCTTTACATCTCCAATAAATCCATTCAAT
ATAAGGGTTGGAGTGATCAAGCTTGTGGTACCCCAGCAAAGGATAGTTNTATATTTGCAA
ACTCTGATGCCACTGGTTTCTATCGTACCCTCTATGATAGCTCAATCTTTGATAAGATTG
TAAATAGACTCAATGAGAATGCCACTGAACATTTCACAACTCTACAAAGAATTTCTTTTG
CTGATGATCTCTATGCATTCTCAAAGATTGGTGCAATTCCAACTTCTGTAGCTTTGAAAA
CTTTTTACTATACCACCGTCTCTGTTGAACCAAATTTCGTAGTTTGGAAAGATATCCTAA
GTTTCCTTTCATTTGTTCACAATCGNTTANAAACCGTTGTTCCATGTTATATTCAATACA
TTAAAAAAGCTCAATXXXXXXXXXXCTTAATCAANTTTATNTAGATAATATTGAGTACAC
CAGAAAATATTGATCCATCAATTAGAGCTCCATTATTCTCATCAATTATACAAAATGGTG
ATATTGAAGAATATGAATGGGTAGTTAATAGATTTGAAAATACAAATGCAATCAATGAAA
GAATCGATGCTTTGAAAGCAATTTCCNATCCAAAGAATCCNCCATTAATTAGAAATACTT
TAAAAATGCTTTTAGATGGTAAAATTAAAAGTCAAGACTTTTATATGGTTTTCTTGGAAA
TGTCATATTCACCATTTGCAAGAGAATTAGCTTGGAACTTTTTATTAGATAATTTTAATT
TCATTTCAGAGAATTCAACACCAGGTGATATTGGTAAATATGTAACCTATTTTGCATCAT
CAATGGATTCCCAAGCAAAAATTGATCAAATTAAACAATTTTTCACAGATGCTCATCCAA
TCCCAGCCTCTTCGTTGGCAAATGCTATATCAAGTATTCAATACAATATGAATTGGTTAA
AAAACCAAGCACCTGAACTTTGTA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
LKSIQSXAIHIVSLQSKFTQPNTTRPRDFNANSIWWMPIKVIDNCENTDDLYISNKSIQY
KGWSDQACGTPAKDSXIFANSDATGFYRTLYDSSIFDKIVNRLNENATEHFTTLQRISFA
DDLYAFSKIGAIPTSVALKTFYYTTVSVEPNFVVWKDILSFLSFVHNRLXTVVPCYIQYI
KKAQ---

---LNQXYXDNIEYTRKY*sin*ssiilinytkw*y*ri*mgs**i*kykcnq*knrcfe
snfxskesxin*kyfknafrw*n*ksrllygflgnviftickrislelfir*f*fhfref
ntr*yw*icnlfciingfpskn*sn*tifhrcssnpslfvgkcyikysiqyelvkkpst*
tl
Translated Amino Acid sequence (All Frames) 
Frame A:
n*nrfnrxqfisyhcnlnslnqiqlvqgismqtlfggcqlksltivktlmtftspinpfn
irvgviklvvpqqrivxylqtlmplvsivpsmiaqslirl*idsmrmplnisqlykefll
lmismhsqrlvqfqll*l*klftippsllnqis*fgkis*vsfhlftixxkplfhvifnt
lkkln---

---LNQXYXDNIEYTRKY*sin*ssiilinytkw*y*ri*mgs**i*kykcnq*knrcfe
snfxskesxin*kyfknafrw*n*ksrllygflgnviftickrislelfir*f*fhfref
ntr*yw*icnlfciingfpskn*sn*tifhrcssnpslfvgkcyikysiqyelvkkpst*
tl

Frame B:
ikidsigsnsyritai*ihstkynsskgfqcklylvdan*sh*ql*kh**plhlq*ihsi
*gle*sslwypskg*xyickl*chwflsypl**lnl**dck*tq*ech*tfhnstknffc
**slcilkdwcnsnfcsfenfllyhrlc*tkfrslerypkfpficsqsxxnrcsmlysih
*kss---

---lixfx*iilstpenidpsiraplfssiiqngdieeyewvvnrfentnaineridalk
aisxpknpplirntlkmlldgkiksqdfymvflemsyspfarelawnflldnfnfisens
tpgdigkyvtyfassmdsqakidqikqfftdahpipasslanaissiqynmnwlknqape
lc

Frame C:
LKSIQSXAIHIVSLQSKFTQPNTTRPRDFNANSIWWMPIKVIDNCENTDDLYISNKSIQY
KGWSDQACGTPAKDSXIFANSDATGFYRTLYDSSIFDKIVNRLNENATEHFTTLQRISFA
DDLYAFSKIGAIPTSVALKTFYYTTVSVEPNFVVWKDILSFLSFVHNRLXTVVPCYIQYI
KKAQ---

---*sxlxr*y*vhqkilihqlelhyshqlykmvilknmng*lidlkiqmqsmkesml*k
qfpiqrixh*leil*kcf*mvklkvktfiwfswkchihhlqen*lgtfy*iilisfqriq
hqvilvnm*pilhhqwipkqkliklnnfsqmliqsqplrwqmlyqvfnti*ig*ktkhln
fv
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFN716 (AFN716Q) /CSM/AF/AFN7-A/AFN716Q.Seq.d/                       2081   0.0
AFN664 (AFN664Q) /CSM/AF/AFN6-C/AFN664Q.Seq.d/                       1053   0.0
SLE171 (SLE171Q) /CSM/SL/SLE1-C/SLE171Q.Seq.d/                       1045   0.0
AFJ551 (AFJ551Q) /CSM/AF/AFJ5-C/AFJ551Q.Seq.d/                       1045   0.0
CHP483 (CHP483Q) /CSM/CH/CHP4-D/CHP483Q.Seq.d/                       1021   0.0
SHL655 (SHL655Q) /CSM/SH/SHL6-C/SHL655Q.Seq.d/                       1001   0.0
CHD548 (CHD548Q) /CSM/CH/CHD5-B/CHD548Q.Seq.d/                       1001   0.0
AHD842 (AHD842Q) /CSM/AH/AHD8-B/AHD842Q.Seq.d/                        983   0.0
CFF794 (CFF794Q) /CSM/CF/CFF7-D/CFF794Q.Seq.d/                        977   0.0
SHD666 (SHD666Q) /CSM/SH/SHD6-C/SHD666Q.Seq.d/                        967   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116330|AC116330.2 Dictyostelium discoideum chromosome 2 map 3191214-3323468 strain AX4, complete sequence.   987  0.0  5
AC012666|AC012666.7 Homo sapiens chromosome 2 clone RP11-219B1, complete sequence.    46  0.19  2
AC113393|AC113393.1 Homo sapiens chromosome 5 clone RP11-3P4, WORKING DRAFT SEQUENCE, 12 unordered pieces.    46  0.30  2
BH468491|BH468491.1 BOHGK91TR BOHG Brassica oleracea genomic clone BOHGK91, DNA sequence.    44  0.41  2
AL512586|AL512586.12 Mouse DNA sequence from clone RP23-37O17 on chromosome 4.    46  0.46  2
AC022950|AC022950.3 Homo sapiens chromosome 1 clone RP11-100A20 map 1, WORKING DRAFT SEQUENCE, 12 unordered pieces.    42  0.62  7
BX572643|BX572643.3 Mouse DNA sequence *** SEQUENCING CANCELLED *** from clone  RP23-303C20.    46  0.93  1
AC139221|AC139221.2 Mus musculus chromosome 16 clone RP24-231D24 map 16, WORKING DRAFT SEQUENCE, 3 unordered pieces.    46  0.93  1
AC009496|AC009496.3 Homo sapiens BAC clone RP11-500G18 from 2, complete sequence.    40  2.3  2
AL713972|AL713972.11 Mouse DNA sequence from clone RP23-305L4 on chromosome X.    38  3.5  4
dna update 2003. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CR380956_8(CR380956|pid:none) Candida glabrata strain CBS138 chr...    83   2e-14
AM270075_67(AM270075|pid:none) Aspergillus niger contig An04c014...    80   9e-14
AP007157_574(AP007157|pid:none) Aspergillus oryzae RIB40 genomic...    80   2e-13
AK031439_1(AK031439|pid:none) Mus musculus 13 days embryo male t...    79   3e-13
(P16406) RecName: Full=Glutamyl aminopeptidase;          EC=3.4....    79   3e-13
AM920433_347(AM920433|pid:none) Penicillium chrysogenum Wisconsi...    79   3e-13
AF327435_1(AF327435|pid:none) Drosophila melanogaster puromycin-...    74   1e-11
AE014296_282(AE014296|pid:none) Drosophila melanogaster chromoso...    73   2e-11
AE014296_281(AE014296|pid:none) Drosophila melanogaster chromoso...    73   2e-11
(P32454) RecName: Full=Aminopeptidase 2, mitochondrial;         ...    71   6e-11
protein update 2009. 4.25
PSORT 

 psg: 0.87  gvh: 0.41  alm: 0.43  top: 0.50  tms: 0.00  mit: 0.44  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: mitochondrial
	 20.0 %: nuclear
	 16.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for AFN716 is mit
5' end seq. ID AFN716F
5' end seq. 
>AFN716F.Seq
AATTAAAATCGATTCAATCGGNAGCAATTCATATCGTATCACTGCAATCTAAATTCACTC
AACCAAATACAACTCGTCCAAGGGATTTCAATGCAAACTCTATTTGGTGGATGCCAATTA
AAGTCATTGACAATTGTGAAAACACTGATGACCTTTACATCTCCAATAAATCCATTCAAT
ATAAGGGTTGGAGTGATCAAGCTTGTGGTACCCCAGCAAAGGATAGTTNTATATTTGCAA
ACTCTGATGCCACTGGTTTCTATCGTACCCTCTATGATAGCTCAATCTTTGATAAGATTG
TAAATAGACTCAATGAGAATGCCACTGAACATTTCACAACTCTACAAAGAATTTCTTTTG
CTGATGATCTCTATGCATTCTCAAAGATTGGTGCAATTCCAACTTCTGTAGCTTTGAAAA
CTTTTTACTATACCACCGTCTCTGTTGAACCAAATTTCGTAGTTTGGAAAGATATCCTAA
GTTTCCTTTCATTTGTTCACAATCGNTTANAAACCGTTGTTCCATGTTATATTCAATACA
TTAAAAAAGCTCAAT----------
Length of 5' end seq. 555
3' end seq. ID AFN716Z
3' end seq. 
>AFN716Z.Seq
----------CTTAATCAANTTTATNTAGATAATATTGAGTACACCAGAAAATATTGATC
CATCAATTAGAGCTCCATTATTCTCATCAATTATACAAAATGGTGATATTGAAGAATATG
AATGGGTAGTTAATAGATTTGAAAATACAAATGCAATCAATGAAAGAATCGATGCTTTGA
AAGCAATTTCCNATCCAAAGAATCCNCCATTAATTAGAAATACTTTAAAAATGCTTTTAG
ATGGTAAAATTAAAAGTCAAGACTTTTATATGGTTTTCTTGGAAATGTCATATTCACCAT
TTGCAAGAGAATTAGCTTGGAACTTTTTATTAGATAATTTTAATTTCATTTCAGAGAATT
CAACACCAGGTGATATTGGTAAATATGTAACCTATTTTGCATCATCAATGGATTCCCAAG
CAAAAATTGATCAAATTAAACAATTTTTCACAGATGCTCATCCAATCCCAGCCTCTTCGT
TGGCAAATGCTATATCAAGTATTCAATACAATATGAATTGGTTAAAAAACCAAGCACCTG
AACTTTGTA
Length of 3' end seq. 539
Connected seq. ID AFN716P
Connected seq. 
>AFN716P.Seq
AATTAAAATCGATTCAATCGGNAGCAATTCATATCGTATCACTGCAATCTAAATTCACTC
AACCAAATACAACTCGTCCAAGGGATTTCAATGCAAACTCTATTTGGTGGATGCCAATTA
AAGTCATTGACAATTGTGAAAACACTGATGACCTTTACATCTCCAATAAATCCATTCAAT
ATAAGGGTTGGAGTGATCAAGCTTGTGGTACCCCAGCAAAGGATAGTTNTATATTTGCAA
ACTCTGATGCCACTGGTTTCTATCGTACCCTCTATGATAGCTCAATCTTTGATAAGATTG
TAAATAGACTCAATGAGAATGCCACTGAACATTTCACAACTCTACAAAGAATTTCTTTTG
CTGATGATCTCTATGCATTCTCAAAGATTGGTGCAATTCCAACTTCTGTAGCTTTGAAAA
CTTTTTACTATACCACCGTCTCTGTTGAACCAAATTTCGTAGTTTGGAAAGATATCCTAA
GTTTCCTTTCATTTGTTCACAATCGNTTANAAACCGTTGTTCCATGTTATATTCAATACA
TTAAAAAAGCTCAAT----------CTTAATCAANTTTATNTAGATAATATTGAGTACAC
CAGAAAATATTGATCCATCAATTAGAGCTCCATTATTCTCATCAATTATACAAAATGGTG
ATATTGAAGAATATGAATGGGTAGTTAATAGATTTGAAAATACAAATGCAATCAATGAAA
GAATCGATGCTTTGAAAGCAATTTCCNATCCAAAGAATCCNCCATTAATTAGAAATACTT
TAAAAATGCTTTTAGATGGTAAAATTAAAAGTCAAGACTTTTATATGGTTTTCTTGGAAA
TGTCATATTCACCATTTGCAAGAGAATTAGCTTGGAACTTTTTATTAGATAATTTTAATT
TCATTTCAGAGAATTCAACACCAGGTGATATTGGTAAATATGTAACCTATTTTGCATCAT
CAATGGATTCCCAAGCAAAAATTGATCAAATTAAACAATTTTTCACAGATGCTCATCCAA
TCCCAGCCTCTTCGTTGGCAAATGCTATATCAAGTATTCAATACAATATGAATTGGTTAA
AAAACCAAGCACCTGAACTTTGTA
Length of connected seq. 1094
Full length Seq ID -
Full length Seq. -
Length of full length seq. -