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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE451
Library VH
(Link to library)
Clone ID VHE451
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16243-1
Original site URL
Representative seq. ID VHE451P
(Link to Original site)
Representative DNA sequence 
>VHE451 (VHE451Q) /CSM/VH/VHE4-C/VHE451Q.Seq.d/
ATATATAATATTACACCCCATAACTAATAAAATGTCATTCAATTCAAGAATTGAAACTAT
TTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTTCTGGATCAGTTAA
ATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTTGTAAAGCTAAAAG
AGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTATTAAACATATTAT
CAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGGGAGCAGTATTACC
AAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAGCAGGTTTACCAAA
GGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTACAAGCAATTGCCAA
TTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAGGCGGTGTAGAATC
AATGAGTTTAAATCCAATGGCATGGGATGGTGGTTTCAACGAGGTTGCCCAAGAGGATCC
AATCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAATGTCGCAGAGCGTTT
TGGTGTCACCAXXXXXXXXXXTGTCGCAGAGCGTTTTGGTGTCACCAGAAAAGACCAAGA
TGAATTCTCTGTAAATTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGA
CGATGAAATCGTTCCAGTCACCGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAA
AGACGAAGGTATCCGTGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTT
CAAAGAGGGTGGTACAACCACCGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGC
TTGTTTAGTTATGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTT
CGTAGCTTTTGCTGCTGTCGGTGTCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGC
AATTCCAGCCGCCTTAAAACAAGCTGGTCTCACCACTAAAGATATCGACGTTTTTGAAAT
CAATGAAGCTTTTGCTTCTCAAGCATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAA
CAAAGTTAATCCAAATGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGC
CCGTCAAATGGCTACCATTTCAAGAGAATTACATCGTCGTAAAGGTAAATATGGTGT
sequence update 2002.10.25
Translated Amino Acid sequence 
YIILHPITNKMSFNSRIETISRHLSTGPILESNPTSGSVKSGDDIVIVAPFRTAICKAKR
GAFKETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVRVATLLAGLPK
EVPCYTVNRQCSSGLQAIANCAAAVKAGFYDIGLAGGVESMSLNPMAWDGGFNEVAQEDP
IISGCYNTMGQTSENVAERFGVT---

---VAERFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVTVKTEDGKTIVVDKDEGI
REKTTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRSTAERLGLKVELVFVAFA
AVGVEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQAFYSCKVLGLDMNKVNP
NGSGIALGHPLGATGARQMATISRELHRRKGKYG
Translated Amino Acid sequence (All Frames) 
Frame A:
iynitphn**nviqfkn*nyfsslkhwtnfriksnfwis*irr*ycncctisysnl*s*k
rsiqrdstr*fistsy*tyyqsyqirskinwrccygssitkiitrcnrs*schiisrftk
gstmlysqqtmfirftsncqlcsssqsrfl*hwfsrrcrinefksngmgwwfqrgcprgs
nh*wllqhygsnl*kcrrafwch---

---crrafwchqkrpr*ilckftqegrcrpksr*i*r*nrsshrkdrgwqnhrrr*rrry
p*enhrcrfgqiktsfqrgwynhrw*l*sne*wcrrlfsyekkyrrkigsqg*igfrsfc
ccrcrtihygywssrcnssrlktswshh*ryrrf*nq*sfcfssilql*gfgfgyeqs*s
kwlwyrfrssircywcpsngyhfkritss*r*iwc

Frame B:
YIILHPITNKMSFNSRIETISRHLSTGPILESNPTSGSVKSGDDIVIVAPFRTAICKAKR
GAFKETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVRVATLLAGLPK
EVPCYTVNRQCSSGLQAIANCAAAVKAGFYDIGLAGGVESMSLNPMAWDGGFNEVAQEDP
IISGCYNTMGQTSENVAERFGVT---

---VAERFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVTVKTEDGKTIVVDKDEGI
REKTTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRSTAERLGLKVELVFVAFA
AVGVEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQAFYSCKVLGLDMNKVNP
NGSGIALGHPLGATGARQMATISRELHRRKGKYG

Frame C:
i*yytp*likchsiqelklflvt*aldqf*nqiqlldqlnqemil*llhhfvqqfvklke
ehskrqhqmiy*hqllnilsklpn*iqn*lemllweqyyqdhhkvqpklelphy*qvyqr
kyhviqstdnvhqvykqlpivqqqskqvsmtlv*qav*nq*v*iqwhgmvvstrlpkriq
slvvvttlwvkplkmsqsvlvsp---

---sqsvlvspektkmnsl*ihtrrqvppkkqvnltmksfqsp*rprmakpsssiktkvs
vrkpplqiwpn*nqlskrvvqpplvtlvk*vmvpplv*l*kevppkdwvsrlnwfs*lll
lsvsnhplwvlvqplqfqpp*nklvsplkistflksmkllllkhftvvrfwvwi*tkliq
malvsl*vih*vllvpvkwlpfqenyivvkvnmv
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE451 (VHE451Q) /CSM/VH/VHE4-C/VHE451Q.Seq.d/                       2432   0.0
VFL606 (VFL606Q) /CSM/VF/VFL6-A/VFL606Q.Seq.d/                       1261   0.0
VFJ317 (VFJ317Q) /CSM/VF/VFJ3-A/VFJ317Q.Seq.d/                       1261   0.0
VFE239 (VFE239Q) /CSM/VF/VFE2-B/VFE239Q.Seq.d/                       1261   0.0
VFD865 (VFD865Q) /CSM/VF/VFD8-C/VFD865Q.Seq.d/                       1261   0.0
SLG762 (SLG762Q) /CSM/SL/SLG7-C/SLG762Q.Seq.d/                       1261   0.0
SFK756 (SFK756Q) /CSM/SF/SFK7-C/SFK756Q.Seq.d/                       1261   0.0
SFJ453 (SFJ453Q) /CSM/SF/SFJ4-C/SFJ453Q.Seq.d/                       1261   0.0
SFI740 (SFI740Q) /CSM/SF/SFI7-B/SFI740Q.Seq.d/                       1261   0.0
SFH501 (SFH501Q) /CSM/SF/SFH5-A/SFH501Q.Seq.d/                       1261   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1261   0.0    1
DY633979|DY633979.1 PU1_plate4_E02 PU1 Prunus persica cDNA similar to acetyl-CoA C-acyltransferase, putative / 3-ketoacyl-CoA thiolase, putative homolog [Arabidopsis thaliana], mRNA sequence.    60   7e-18  3
CA917334|CA917334.1 EST641481 GPOD Medicago truncatula cDNA clone GPOD-35H18, mRNA sequence.    40   7e-18  7
AR547531|AR547531.1 Sequence 2662 from patent US 6747137.          50   3e-16  5
CA858588|CA858588.1 EST635843 GLSD Medicago truncatula cDNA clone pGLSD-38M16, mRNA sequence.    40   6e-14  6
DY638135|DY638135.1 PU2_plate5_K13 PU2 Prunus persica cDNA similar to acetyl-CoA C-acyltransferase, putative / 3-ketoacyl-CoA thiolase, putative homolog [Arabidopsis thaliana], mRNA sequence.    60   9e-14  2
DY637544|DY637544.1 PU2_plate14_K10 PU2 Prunus persica cDNA similar to acetyl-CoA C-acyltransferase, putative / 3-ketoacyl-CoA thiolase, putative homolog [Arabidopsis thaliana], mRNA sequence.    60   9e-14  2
DY642152|DY642152.1 PU3_plate6_P14 PU3 Prunus persica cDNA similar to acetyl-CoA C-acyltransferase, putative / 3-ketoacyl-CoA thiolase, putative homolog [Arabidopsis thaliana], mRNA sequence.    60   9e-14  2
DY648712|DY648712.1 PU4_plate11_J11 PU4 Prunus persica cDNA similar to similar to acetyl-CoA C-acyltransferase, putative / 3-ketoacyl-CoA thiolase, putative [Arabidopsis thaliana], mRNA sequence.    60   9e-14  2
DY639053|DY639053.1 PU2_plate36_O19 PU2 Prunus persica cDNA similar to acetyl-CoA C-acyltransferase, putative / 3-ketoacyl-CoA thiolase, putative homolog [Arabidopsis thaliana], mRNA sequence.    60   9e-14  2
dna update 2007. 7.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116986_75(AC116986|pid:none) Dictyostelium discoideum chromoso...   743   0.0
X75329_1(X75329|pid:none) M.indica (Manila) THMF5 mRNA for 3-ket...   403   e-111
AM474896_9(AM474896|pid:none) Vitis vinifera contig VV79X001698....   402   e-110
S57792(S57792)acetyl-CoA C-acyltransferase (EC 2.3.1.16) B precu...   401   e-110
AB333797_1(AB333797|pid:none) Glycine max mRNA for peroxisomal 3...   397   e-109
T07989(T07989) acetyl-CoA C-acyltransferase (EC 2.3.1.16) precur...   396   e-109
(Q56WD9) RecName: Full=3-ketoacyl-CoA thiolase 2, peroxisomal;  ...   396   e-109
EU964831_1(EU964831|pid:none) Zea mays clone 281881 3-ketoacyl-C...   394   e-108
BT034496_1(BT034496|pid:none) Zea mays full-length cDNA clone ZM...   394   e-108
BT070433_1(BT070433|pid:none) Picea sitchensis clone WS02729_F15...   394   e-108
protein update 2009. 6.30
PSORT 

 psg: 0.61  gvh: 0.40  alm: 0.28  top: 0.33  tms: 0.07  mit: 0.35  mip: 0.05
 nuc: 0.02  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.03  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: mitochondrial
	 24.0 %: nuclear
	 20.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal

>> prediction for VHE451 is mit
5' end seq. ID VHE451F
5' end seq. 
>VHE451F.Seq
ATATATAATATTACACCCCATAACTAATAAAATGTCATTCAATTCAAGAATTGAAACTAT
TTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTTCTGGATCAGTTAA
ATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTTGTAAAGCTAAAAG
AGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTATTAAACATATTAT
CAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGGGAGCAGTATTACC
AAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAGCAGGTTTACCAAA
GGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTACAAGCAATTGCCAA
TTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAGGCGGTGTAGAATC
AATGAGTTTAAATCCAATGGCATGGGATGGTGGTTTCAACGAGGTTGCCCAAGAGGATCC
AATCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAATGTCGCAGAGCGTTT
TGGTGTCACCANNNNNNNNNN
Length of 5' end seq. 621
3' end seq. ID VHE451Z
3' end seq. 
>VHE451Z.Seq
NNNNNNNNNNTGTCGCAGAGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAATTCTCTG
TAAATTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGATGAAATCG
TTCCAGTCACCGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGACGAAGGTA
TCCGTGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAAGAGGGTG
GTACAACCACCGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGTTTAGTTA
TGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTAGCTTTTG
CTGCTGTCGGTGTCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATTCCAGCCG
CCTTAAAACAAGCTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAATGAAGCTT
TTGCTTCTCAAGCATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAAAGTTAATC
CAAATGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGTCAAATGG
CTACCATTTCAAGAGAATTACATCGTCGTAAAGGTAAATATGGTGT
Length of 3' end seq. 646
Connected seq. ID VHE451P
Connected seq. 
>VHE451P.Seq
ATATATAATATTACACCCCATAACTAATAAAATGTCATTCAATTCAAGAATTGAAACTAT
TTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTTCTGGATCAGTTAA
ATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTTGTAAAGCTAAAAG
AGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTATTAAACATATTAT
CAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGGGAGCAGTATTACC
AAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAGCAGGTTTACCAAA
GGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTACAAGCAATTGCCAA
TTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAGGCGGTGTAGAATC
AATGAGTTTAAATCCAATGGCATGGGATGGTGGTTTCAACGAGGTTGCCCAAGAGGATCC
AATCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAATGTCGCAGAGCGTTT
TGGTGTCACCA----------TGTCGCAGAGCGTTTTGGTGTCACCAGAAAAGACCAAGA
TGAATTCTCTGTAAATTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGA
CGATGAAATCGTTCCAGTCACCGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAA
AGACGAAGGTATCCGTGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTT
CAAAGAGGGTGGTACAACCACCGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGC
TTGTTTAGTTATGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTT
CGTAGCTTTTGCTGCTGTCGGTGTCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGC
AATTCCAGCCGCCTTAAAACAAGCTGGTCTCACCACTAAAGATATCGACGTTTTTGAAAT
CAATGAAGCTTTTGCTTCTCAAGCATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAA
CAAAGTTAATCCAAATGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGC
CCGTCAAATGGCTACCATTTCAAGAGAATTACATCGTCGTAAAGGTAAATATGGTGT
Length of connected seq. 1247
Full length Seq ID -
Full length Seq. -
Length of full length seq. -