>VHE439 (VHE439Q) /CSM/VH/VHE4-B/VHE439Q.Seq.d/
AACTCTCGAGTGCAAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATT
CGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGAT
CACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAA
GTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACC
ATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCT
GGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTC
AGTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGC
GTCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATC
AAACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACT
GAAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAAC
ACTGAAGATXXXXXXXXXX

NSRVQKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADK
VSGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDF
SSEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNT
EEAYLSFRRAIESVNVIVGNTE---

Frame A:
NSRVQKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADK
VSGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDF
SSEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNT
EEAYLSFRRAIESVNVIVGNTE---


Frame B:
tleckkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlk---


Frame C:
lssakkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                       1166   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                       1166   0.0
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                       1166   0.0
VHQ469 (VHQ469Q) /CSM/VH/VHQ4-C/VHQ469Q.Seq.d/                       1166   0.0
VHQ463 (VHQ463Q) /CSM/VH/VHQ4-C/VHQ463Q.Seq.d/                       1166   0.0
VHQ453 (VHQ453Q) /CSM/VH/VHQ4-C/VHQ453Q.Seq.d/                       1166   0.0
VHQ446 (VHQ446Q) /CSM/VH/VHQ4-B/VHQ446Q.Seq.d/                       1166   0.0
VHQ441 (VHQ441Q) /CSM/VH/VHQ4-B/VHQ441Q.Seq.d/                       1166   0.0
VHQ438 (VHQ438Q) /CSM/VH/VHQ4-B/VHQ438Q.Seq.d/                       1166   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.  1164   0.0    1
CX155337|CX155337.1 TfEST072F03 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    90   2e-34  4
CX154324|CX154324.1 TfEST033C12 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    90   4e-34  4
CX154971|CX154971.1 TfEST089A07 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    90   1e-33  4
CX154356|CX154356.1 TfEST029E10 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    90   1e-33  4
DY889811|DY889811.1 CeleSEQ6761 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ6761, mRNA sequence.    84   6e-33  4
DY889772|DY889772.1 CeleSEQ11980 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ11980, mRNA sequence.    84   6e-33  4
DY890935|DY890935.1 CeleSEQ7425 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ7425, mRNA sequence.    84   9e-33  4
DY887441|DY887441.1 CeleSEQ3766 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ3766, mRNA sequence.    84   9e-33  4
DY888936|DY888936.1 CeleSEQ6366 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ6366, mRNA sequence.    84   1e-32  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   264   2e-69
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   259   3e-68
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   258   9e-68
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   258   1e-67
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   257   2e-67
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   257   2e-67
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   256   5e-67
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   254   1e-66
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   254   1e-66
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   254   1e-66

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.38  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 28.0 %: mitochondrial
	 28.0 %: nuclear
	 24.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VHE439 is mit

>VHE439F.Seq
AACTCTCGAGTGCAAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATT
CGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGAT
CACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAA
GTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACC
ATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCT
GGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTC
AGTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGC
GTCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATC
AAACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACT
GAAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAAC
ACTGAAGATNNNNNNNNNN