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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE418
Library VH
(Link to library)
Clone ID VHE418
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12694-1
Original site URL
Representative seq. ID VHE418P
(Link to Original site)
Representative DNA sequence 
>VHE418 (VHE418Q) /CSM/VH/VHE4-A/VHE418Q.Seq.d/
AAATATATAAATCNACCATTATATTATATATATATATATAATTTTTTTTTAATATTATTT
TTTTAAAAAAAAAGAATGATTAATTCTTTTAAAAAATTAATTATTCTTATTTCATTGGNG
ATTATTTTACTTTCATCGAATAATATTTTCATAGATTCATTTAAAATTCCAGTTAATCAA
AAAAATGTTAAATCAAGTGGTGATTCATCATATCAAATAGGTGCCGGTATTTATGATATC
ACTGGTGCAAGCGCTGAAGTTAATTTAATGGGTTATGCTAATCCACTTCAAGTTGGTGCA
GGTATTCATTTTCGTCAAAGAGCACGTGCCTTTGTTTTTGTANATTCAAATGGTAATANA
GCTGTATATGTTANTACTGATTCATGTATGATTTTCCAANAAGTTAAAATTCATGTGGCT
GAATTGCTACAANATATTTTTGGACCAAATGTTTATACAGAGGCAAATGTATTATTAAGT
GGTACTCATACTCATTCTGGTXXXXXXXXXXTGTCTGGTAGAAGATTAAGAAATAGTGTT
AGAGAAATCATTGGGGAATCAATTGAAAATCCAATTGTTTTAATTGCTGGTCTTTCCAAT
ACTTATTCTGGTTATATAGCAACATTTGAAGAATATCAAGTTCAAAGATATGAGGGTGCA
TCAACTGTTTTTGGTCCACATACTCTTGGTTCATATATGCAAGAGTTTGGAAAACTTGCA
CAATCAATTGTTGATGGAACAACAGTTCCTGCAGGTCCAACTCCAAGAAATTTAACAGGT
CACACACTTTTCTTTTTACCACCAGTAATTGTAGATGCCGCACCAGATTTTGATGATTTT
GGTGAAGTTTCAATTGATGTCAATNTTAATTATTCAGTAAATGAAACAGTATCATGTGTT
TTTTATGGTGGTAATCCTAGAAATGATTTTATGATTGAATCATCATTCTTATCAGTTGAT
TTATTGACAGGTACAGATCAATGGACAACAGTTTTAGATGATGGTGATTGGGATACAAAA
TTCAAATGGAAAATGCACNATTTAGGTTTTTCTTTAATTACTATAGAATGGGTTATTGCT
CCAGATACAACACCAGGTACTTATAGAATAACTCATAGTGGTTTTGCTAAAAAGAATCCT
TTCTCTTCAAATTTAACTCCGTATCAAGGTATCTCAAGAAATTTTGTAGTTCAATAAAG
sequence update 2002.10.25
Translated Amino Acid sequence 
kyinxplyyiyiynfflilff*KKRMINSFKKLIILISLXIILLSSNNIFIDSFKIPVNQ
KNVKSSGDSSYQIGAGIYDITGASAEVNLMGYANPLQVGAGIHFRQRARAFVFVXSNGNX
AVYVXTDSCMIFQXVKIHVAELLQXIFGPNVYTEANVLLSGTHTHSG---

---SGRRLRNSVREIIGESIENPIVLIAGLSNTYSGYIATFEEYQVQRYEGASTVFGPHT
LGSYMQEFGKLAQSIVDGTTVPAGPTPRNLTGHTLFFLPPVIVDAAPDFDDFGEVSIDVN
XNYSVNETVSCVFYGGNPRNDFMIESSFLSVDLLTGTDQWTTVLDDGDWDTKFKWKMHXL
GFSLITIEWVIAPDTTPGTYRITHSGFAKKNPFSSNLTPYQGISRNFVVQ*
Translated Amino Acid sequence (All Frames) 
Frame A:
kyinxplyyiyiynfflilff*KKRMINSFKKLIILISLXIILLSSNNIFIDSFKIPVNQ
KNVKSSGDSSYQIGAGIYDITGASAEVNLMGYANPLQVGAGIHFRQRARAFVFVXSNGNX
AVYVXTDSCMIFQXVKIHVAELLQXIFGPNVYTEANVLLSGTHTHSG---

---clved*eivlekslgnqlkiqlf*llvfpililvi*qhlknikfkdmrvhqlflvhi
llvhickslenlhnqllmeqqflqvqlqei*qvthfsfyhq*l*mphqilmilvkfqlms
xliiq*mkqyhvffmvvilemil*lnhhsyqliy*qvqingqqf*mmvigiqnsngkcti
*vfl*ll*nglllqiqhqvlie*livvllkrilslqi*lrikvsqeil*fnk

Frame B:
ni*ixhyiiyiyiiff*yyffkkke*lillkn*lflfhwxlfyfhriifs*ihlkfqlik
kmlnqvvihhik*vpvfmislvqalkli*wvmlihfklvqvfifvkehvplflxiqmvix
lymlxlihv*fsxklkfmwlncyxifldqmfiqrqmyy*vvlilil---

---vw*kikk*c*rnhwgin*ksncfncwsfqylfwlysni*rissski*gcincfwsty
swfiyarvwktctinc*wnnsscrsnskkfnrshtflfttsncrcrtrf**fw*sfn*cq
x*lfsk*nsimcflww*s*k*fyd*iiilis*fidryrsmdnsfr*w*lgykiqmenaxf
rfffnyyrmgycsryntryl*nns*wfc*kesflfkfnsvsrylkkfcssik

Frame C:
iykstiilyiyi*fffniiflkkknd*ff*kinysyfigdyftfie*yfhrfi*nss*sk
kc*ikw*fiisnrcryl*yhwckr*s*fnglc*stsswcrysfsskstclcfcxfkw*xs
cicxy*fmydfpxs*nscg*iatxyfwtkclyrgkciikwysysfw---

---SGRRLRNSVREIIGESIENPIVLIAGLSNTYSGYIATFEEYQVQRYEGASTVFGPHT
LGSYMQEFGKLAQSIVDGTTVPAGPTPRNLTGHTLFFLPPVIVDAAPDFDDFGEVSIDVN
XNYSVNETVSCVFYGGNPRNDFMIESSFLSVDLLTGTDQWTTVLDDGDWDTKFKWKMHXL
GFSLITIEWVIAPDTTPGTYRITHSGFAKKNPFSSNLTPYQGISRNFVVQ*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE418 (VHE418Q) /CSM/VH/VHE4-A/VHE418Q.Seq.d/                       2083   0.0
AHM667 (AHM667Q) /CSM/AH/AHM6-C/AHM667Q.Seq.d/                       1344   0.0
AFE563 (AFE563Q) /CSM/AF/AFE5-C/AFE563Q.Seq.d/                       1344   0.0
VHC444 (VHC444Q) /CSM/VH/VHC4-B/VHC444Q.Seq.d/                       1340   0.0
VHI778 (VHI778Q) /CSM/VH/VHI7-D/VHI778Q.Seq.d/                       1332   0.0
CHS327 (CHS327Q) /CSM/CH/CHS3-B/CHS327Q.Seq.d/                       1332   0.0
AHF544 (AHF544Q) /CSM/AH/AHF5-B/AHF544Q.Seq.d/                       1332   0.0
VHJ717 (VHJ717Q) /CSM/VH/VHJ7-A/VHJ717Q.Seq.d/                       1330   0.0
AHK614 (AHK614Q) /CSM/AH/AHK6-A/AHK614Q.Seq.d/                       1330   0.0
AHH613 (AHH613Q) /CSM/AH/AHH6-A/AHH613Q.Seq.d/                       1328   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U82513|U82513.1 Dictyostelium discoideum random slug cDNA25 protein (rsc25) mRNA, partial cds.   216   e-140  8
AB121061|AB121061.1 Dictyostelium discoideum dcd2A mRNA for ceramidase, complete cds.   216   e-140  8
AY057506|AY057506.1 Arabidopsis thaliana AT5g58980/k19m22_180 mRNA, complete cds.    60   2e-07  3
EG499304|EG499304.1 AYBLC10TR pooled cDNA populations Arabidopsis thaliana cDNA, mRNA sequence.    60   2e-04  1
DT593421|DT593421.1 wmi03-8ms3-c10 Wmi03 Welwitschia mirabilis cDNA clone wmi03-8ms3-c10 5', mRNA sequence.    58   6e-04  1
CD141720|CD141720.1 MG1-0071U-A276-B06-U.B MG1-0071 Schistosoma mansoni cDNA clone MG1-0071U-A276-B06.B, mRNA sequence.    56   0.002  1
CJ529838|CJ529838.1 Triticum aestivum cDNA clone rwhec12i09 3', Y.Ogihara unpublished cDNA library Wh_EMC, mRNA sequence.    56   0.002  1
DY893037|DY893037.1 CeleSEQ12200 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ12200, mRNA sequence.    54   0.010  1
CK161403|CK161403.1 FGAS013973 Triticum aestivum FGAS: Library 4 Gate 8 Triticum aestivum cDNA, mRNA sequence.    54   0.010  1
CJ522023|CJ522023.1 Triticum aestivum cDNA clone rwhcs4i13 3', Y.Ogihara unpublished cDNA library Wh_CSEC, mRNA sequence.    54   0.010  1
dna update 2007. 7.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55G11) RecName: Full=Neutral ceramidase B;          Short=N-CD...   463   e-129
U82513_1(U82513|pid:none) Dictyostelium discoideum random slug c...   352   2e-95
EU200974_1(EU200974|pid:none) Triticum aestivum neutral ceramida...   187   6e-46
CP000777_2353(CP000777|pid:none) Leptospira biflexa serovar Pato...   182   2e-44
AP002819_24(AP002819|pid:none) Oryza sativa Japonica Group genom...   182   2e-44
AK099625_1(AK099625|pid:none) Oryza sativa Japonica Group cDNA c...   182   2e-44
(Q304B9) RecName: Full=Neutral ceramidase;          Short=N-CDas...   181   3e-44
AC022464_28(AC022464|pid:none) Genomic sequence for Arabidopsis ...   179   2e-43
AB016885_17(AB016885|pid:none) Arabidopsis thaliana genomic DNA,...   173   1e-41
BC167651_1(BC167651|pid:none) Xenopus tropicalis hypothetical pr...   170   1e-40
protein update 2009. 6.30
PSORT 

 psg: 0.95  gvh: 0.62  alm: 0.27  top: 0.27  tms: 0.07  mit: 0.42  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 40.0 %: mitochondrial
	 24.0 %: Golgi
	 16.0 %: nuclear
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoplasmic
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall

>> prediction for VHE418 is mit
5' end seq. ID VHE418F
5' end seq. 
>VHE418F.Seq
AAATATATAAATCNACCATTATATTATATATATATATATAATTTTTTTTTAATATTATTT
TTTTAAAAAAAAAGAATGATTAATTCTTTTAAAAAATTAATTATTCTTATTTCATTGGNG
ATTATTTTACTTTCATCGAATAATATTTTCATAGATTCATTTAAAATTCCAGTTAATCAA
AAAAATGTTAAATCAAGTGGTGATTCATCATATCAAATAGGTGCCGGTATTTATGATATC
ACTGGTGCAAGCGCTGAAGTTAATTTAATGGGTTATGCTAATCCACTTCAAGTTGGTGCA
GGTATTCATTTTCGTCAAAGAGCACGTGCCTTTGTTTTTGTANATTCAAATGGTAATANA
GCTGTATATGTTANTACTGATTCATGTATGATTTTCCAANAAGTTAAAATTCATGTGGCT
GAATTGCTACAANATATTTTTGGACCAAATGTTTATACAGAGGCAAATGTATTATTAAGT
GGTACTCATACTCATTCTGGTNNNNNNNNNN
Length of 5' end seq. 511
3' end seq. ID VHE418Z
3' end seq. 
>VHE418Z.Seq
NNNNNNNNNNTGTCTGGTAGAAGATTAAGAAATAGTGTTAGAGAAATCATTGGGGAATCA
ATTGAAAATCCAATTGTTTTAATTGCTGGTCTTTCCAATACTTATTCTGGTTATATAGCA
ACATTTGAAGAATATCAAGTTCAAAGATATGAGGGTGCATCAACTGTTTTTGGTCCACAT
ACTCTTGGTTCATATATGCAAGAGTTTGGAAAACTTGCACAATCAATTGTTGATGGAACA
ACAGTTCCTGCAGGTCCAACTCCAAGAAATTTAACAGGTCACACACTTTTCTTTTTACCA
CCAGTAATTGTAGATGCCGCACCAGATTTTGATGATTTTGGTGAAGTTTCAATTGATGTC
AATNTTAATTATTCAGTAAATGAAACAGTATCATGTGTTTTTTATGGTGGTAATCCTAGA
AATGATTTTATGATTGAATCATCATTCTTATCAGTTGATTTATTGACAGGTACAGATCAA
TGGACAACAGTTTTAGATGATGGTGATTGGGATACAAAATTCAAATGGAAAATGCACNAT
TTAGGTTTTTCTTTAATTACTATAGAATGGGTTATTGCTCCAGATACAACACCAGGTACT
TATAGAATAACTCATAGTGGTTTTGCTAAAAAGAATCCTTTCTCTTCAAATTTAACTCCG
TATCAAGGTATCTCAAGAAATTTTGTAGTTCAATAAAG
Length of 3' end seq. 698
Connected seq. ID VHE418P
Connected seq. 
>VHE418P.Seq
AAATATATAAATCNACCATTATATTATATATATATATATAATTTTTTTTTAATATTATTT
TTTTAAAAAAAAAGAATGATTAATTCTTTTAAAAAATTAATTATTCTTATTTCATTGGNG
ATTATTTTACTTTCATCGAATAATATTTTCATAGATTCATTTAAAATTCCAGTTAATCAA
AAAAATGTTAAATCAAGTGGTGATTCATCATATCAAATAGGTGCCGGTATTTATGATATC
ACTGGTGCAAGCGCTGAAGTTAATTTAATGGGTTATGCTAATCCACTTCAAGTTGGTGCA
GGTATTCATTTTCGTCAAAGAGCACGTGCCTTTGTTTTTGTANATTCAAATGGTAATANA
GCTGTATATGTTANTACTGATTCATGTATGATTTTCCAANAAGTTAAAATTCATGTGGCT
GAATTGCTACAANATATTTTTGGACCAAATGTTTATACAGAGGCAAATGTATTATTAAGT
GGTACTCATACTCATTCTGGT----------TGTCTGGTAGAAGATTAAGAAATAGTGTT
AGAGAAATCATTGGGGAATCAATTGAAAATCCAATTGTTTTAATTGCTGGTCTTTCCAAT
ACTTATTCTGGTTATATAGCAACATTTGAAGAATATCAAGTTCAAAGATATGAGGGTGCA
TCAACTGTTTTTGGTCCACATACTCTTGGTTCATATATGCAAGAGTTTGGAAAACTTGCA
CAATCAATTGTTGATGGAACAACAGTTCCTGCAGGTCCAACTCCAAGAAATTTAACAGGT
CACACACTTTTCTTTTTACCACCAGTAATTGTAGATGCCGCACCAGATTTTGATGATTTT
GGTGAAGTTTCAATTGATGTCAATNTTAATTATTCAGTAAATGAAACAGTATCATGTGTT
TTTTATGGTGGTAATCCTAGAAATGATTTTATGATTGAATCATCATTCTTATCAGTTGAT
TTATTGACAGGTACAGATCAATGGACAACAGTTTTAGATGATGGTGATTGGGATACAAAA
TTCAAATGGAAAATGCACNATTTAGGTTTTTCTTTAATTACTATAGAATGGGTTATTGCT
CCAGATACAACACCAGGTACTTATAGAATAACTCATAGTGGTTTTGCTAAAAAGAATCCT
TTCTCTTCAAATTTAACTCCGTATCAAGGTATCTCAAGAAATTTTGTAGTTCAATAAAG
Length of connected seq. 1189
Full length Seq ID -
Full length Seq. -
Length of full length seq. -