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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE404
Library VH
(Link to library)
Clone ID VHE404
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16292-1|Contig-U16400-1
Original site URL
Representative seq. ID VHE404P
(Link to Original site)
Representative DNA sequence 
>VHE404 (VHE404Q) /CSM/VH/VHE4-A/VHE404Q.Seq.d/
AATTTAAACAATATATAAATCAATTAAAATAAACACTCACTCGATAGTGTAAAAGAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNNTTTTNNAAAAAAAAX
XXXXXXXXXTGCTGAACGTTCATATATAATGGAAAGAGGTATCACATTGAATACACTAAC
CAAGGATTTGGAAATGTTTGTTGAACGTTTCAAACGTGAACATAGAATTGGTCTAATTAT
TAAGAGTGAACTTGCTAGCAACACCTACTCAACTCATTTCATTTATTCACTCTTCAAAGA
AGAAGGTAAACATTTATTCGATGTCCGTGAATCTATACTCGGTCATTTACAACAAGGTGG
TACTCCTTCTGCAATTGATCGTATCTTTTCAACTCGTTTAATGAATCATTATTATCAATT
CTTGGAAAACGATCTCAAAGAACATGGTCATCTTCAAATGAATGGTTGTATTGGTTTCAT
TGATGGTGGTATTCATTACACTCCAATGCAAGAAATGATCGAAGAAATGTCTGATAAATT
TAGACGTCCTCGTTCCCAATGGTGGATGGATTTGGTTGAAACCTCTCAAAATATCTCTGT
TTTCCCTTTAGATGACCCTTCCTCT
sequence update 2002.10.25
Translated Amino Acid sequence 
nlnni*in*NKHSLDSVKEKKKKKKKKKKKKKKKXFXKK---

---AERSYIMERGITLNTLTKDLEMFVERFKREHRIGLIIKSELASNTYSTHFIYSLFKE
EGKHLFDVRESILGHLQQGGTPSAIDRIFSTRLMNHYYQFLENDLKEHGHLQMNGCIGFI
DGGIHYTPMQEMIEEMSDKFRRPRSQWWMDLVETSQNISVFPLDDPSS
Translated Amino Acid sequence (All Frames) 
Frame A:
nlnni*in*NKHSLDSVKEKKKKKKKKKKKKKKKXFXKK---

---c*tfiyngkryhieytnqgfgnvc*tfqt*t*nwsny*e*tc*qhllnsfhlftlqr
rr*tfircp*iytrsfttrwysfcn*sylfnsfnesllsilgkrsqrtwsssnewlywfh
*wwyslhsnarndrrnv**i*tssfpmvdgfg*nlskylcfpfr*pfl

Frame B:
i*tiyksikinthsiv*kkkkkkkkkkkkkkkkxxfxkk---

---AERSYIMERGITLNTLTKDLEMFVERFKREHRIGLIIKSELASNTYSTHFIYSLFKE
EGKHLFDVRESILGHLQQGGTPSAIDRIFSTRLMNHYYQFLENDLKEHGHLQMNGCIGFI
DGGIHYTPMQEMIEEMSDKFRRPRSQWWMDLVETSQNISVFPLDDPSS

Frame C:
fkqyinqlk*tltr*ckrkkkkkkkkkkkkkkkxxxxkk---

---lnvhi*wkevsh*ih*priwkcllnvsnvnielv*llrvnllatptqlisfihsskk
kvniysmsvnlysviynkvvlllqlivsfqlv**iiiinswktisknmvifk*mvvlvsl
mvvfitlqckk*skkclinldvlvpnggwiwlkplkislfsl*mtlp
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP708 (VHP708Q) /CSM/VH/VHP7-A/VHP708Q.Seq.d/                        983   0.0
VHP463 (VHP463Q) /CSM/VH/VHP4-C/VHP463Q.Seq.d/                        983   0.0
VHO736 (VHO736Q) /CSM/VH/VHO7-B/VHO736Q.Seq.d/                        983   0.0
VHO650 (VHO650Q) /CSM/VH/VHO6-C/VHO650Q.Seq.d/                        983   0.0
VHO434 (VHO434Q) /CSM/VH/VHO4-B/VHO434Q.Seq.d/                        983   0.0
VHN894 (VHN894Q) /CSM/VH/VHN8-D/VHN894Q.Seq.d/                        983   0.0
VHN560 (VHN560Q) /CSM/VH/VHN5-C/VHN560Q.Seq.d/                        983   0.0
VHN457 (VHN457Q) /CSM/VH/VHN4-C/VHN457Q.Seq.d/                        983   0.0
VHK413 (VHK413Q) /CSM/VH/VHK4-A/VHK413Q.Seq.d/                        983   0.0
VHJ854 (VHJ854Q) /CSM/VH/VHJ8-C/VHJ854Q.Seq.d/                        983   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   983   0.0    2
X94340|X94340.1 D.discoideum mRNA for 6-phosphofructo-1-kinase.   983   0.0    1
X89039|X89039.1 D.discoideum mRNA for 6-phosphofructo-1-kinase.   983   0.0    1
AJ430522|AJ430522.1 Dictyostelium discoideum partial pfk gene for ATP:D-fructose  6-phosphate 1-phosphofructokinase, exon 1.   109   1e-19  1
AC095674|AC095674.6 Rattus norvegicus clone CH230-8J14, WORKING DRAFT SEQUENCE, 2 unordered pieces.    50   0.077  1
DX414156|DX414156.1 SOYC248TH LargeInsertSoybeanGenLibBuild4 Glycine max genomic clone H12A17:Build4MTP4H23, genomic survey sequence.    48   0.30   1
EJ712224|EJ712224.1 1092956083569 Global-Ocean-Sampling_GS-30-02-01-1P0-1P3KB marine metagenome genomic clone 1061001798086 3', genomic survey sequence.    46   1.2    1
EJ705753|EJ705753.1 1092956052585 Global-Ocean-Sampling_GS-30-02-01-1P0-1P3KB marine metagenome genomic clone 1061001798086 5', genomic survey sequence.    46   1.2    1
EI048740|EI048740.1 hhd0006bb09.b1 hhd Oryza coarctata genomic clone hhd0006bb09, genomic survey sequence.    46   1.2    1
CE635221|CE635221.1 tigr-gss-dog-17000366743799 Dog Library Canis familiaris genomic, genomic survey sequence.    46   1.2    1
dna update 2007. 7.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P90521) RecName: Full=6-phosphofructokinase;          Short=Pho...   340   1e-92
CP001337_2617(CP001337|pid:none) Chloroflexus aggregans DSM 9485...   126   6e-28
BC081652_1(BC081652|pid:none) Danio rerio phosphofructokinase, m...   110   3e-23
(Q867C9) RecName: Full=6-phosphofructokinase, muscle type;      ...   106   5e-22
BC077756_1(BC077756|pid:none) Xenopus laevis MGC79063 protein, m...   105   8e-22
DQ480147_1(DQ480147|pid:none) Sus scrofa muscle 6-phosphofructok...   105   1e-21
AY335614_1(AY335614|pid:none) Synthetic construct Homo sapiens p...   104   2e-21
CR858939_1(CR858939|pid:none) Pongo abelii mRNA; cDNA DKFZp468O1...   104   2e-21
(P08237) RecName: Full=6-phosphofructokinase, muscle type;      ...   104   2e-21
(Q0IIG5) RecName: Full=6-phosphofructokinase, muscle type;      ...   104   2e-21
protein update 2009. 6.30
PSORT 

 psg: 0.03  gvh: 0.25  alm: 0.54  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.64  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: cytoplasmic
	  4.0 %: mitochondrial

>> prediction for VHE404 is nuc
5' end seq. ID VHE404F
5' end seq. 
>VHE404F.Seq
AATTTAAACAATATATAAATCAATTAAAATAAACACTCACTCGATAGTGTAAAAGAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNNTTTTNNAAAAAAAAN
NNNNNNNNN
Length of 5' end seq. 129
3' end seq. ID VHE404Z
3' end seq. 
>VHE404Z.Seq
NNNNNNNNNNTGCTGAACGTTCATATATAATGGAAAGAGGTATCACATTGAATACACTAA
CCAAGGATTTGGAAATGTTTGTTGAACGTTTCAAACGTGAACATAGAATTGGTCTAATTA
TTAAGAGTGAACTTGCTAGCAACACCTACTCAACTCATTTCATTTATTCACTCTTCAAAG
AAGAAGGTAAACATTTATTCGATGTCCGTGAATCTATACTCGGTCATTTACAACAAGGTG
GTACTCCTTCTGCAATTGATCGTATCTTTTCAACTCGTTTAATGAATCATTATTATCAAT
TCTTGGAAAACGATCTCAAAGAACATGGTCATCTTCAAATGAATGGTTGTATTGGTTTCA
TTGATGGTGGTATTCATTACACTCCAATGCAAGAAATGATCGAAGAAATGTCTGATAAAT
TTAGACGTCCTCGTTCCCAATGGTGGATGGATTTGGTTGAAACCTCTCAAAATATCTCTG
TTTTCCCTTTAGATGACCCTTCCTCT
Length of 3' end seq. 506
Connected seq. ID VHE404P
Connected seq. 
>VHE404P.Seq
AATTTAAACAATATATAAATCAATTAAAATAAACACTCACTCGATAGTGTAAAAGAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNNTTTTNNAAAAAAAA-
---------TGCTGAACGTTCATATATAATGGAAAGAGGTATCACATTGAATACACTAAC
CAAGGATTTGGAAATGTTTGTTGAACGTTTCAAACGTGAACATAGAATTGGTCTAATTAT
TAAGAGTGAACTTGCTAGCAACACCTACTCAACTCATTTCATTTATTCACTCTTCAAAGA
AGAAGGTAAACATTTATTCGATGTCCGTGAATCTATACTCGGTCATTTACAACAAGGTGG
TACTCCTTCTGCAATTGATCGTATCTTTTCAACTCGTTTAATGAATCATTATTATCAATT
CTTGGAAAACGATCTCAAAGAACATGGTCATCTTCAAATGAATGGTTGTATTGGTTTCAT
TGATGGTGGTATTCATTACACTCCAATGCAAGAAATGATCGAAGAAATGTCTGATAAATT
TAGACGTCCTCGTTCCCAATGGTGGATGGATTTGGTTGAAACCTCTCAAAATATCTCTGT
TTTCCCTTTAGATGACCCTTCCTCT
Length of connected seq. 615
Full length Seq ID -
Full length Seq. -
Length of full length seq. -