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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE353
Library VH
(Link to library)
Clone ID VHE353
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10840-1
Original site URL
Representative seq. ID VHE353P
(Link to Original site)
Representative DNA sequence 
>VHE353 (VHE353Q) /CSM/VH/VHE3-C/VHE353Q.Seq.d/
AATTTTTATAGATTTTGTTATTTTAGTCATCAATATTGTGTGTATATGTATAATAATTGT
GTATATTAGTGTATAATATATATCTATTTTTAAACAGATCAACAATAAGAAAAAAATGAA
AAGATCAGAAAAATCAAGTACATCTGTTGTTAGTAATAACAAACAACAAGATGTAAATAT
CATCAGTTCAAATGAAGTTGGTGTTAAAGAAGAAAATAAAGGACATCAAGAATTCTTATT
AAAAGTTTTAATTCTATCAGTCATTTATGTTTTAGCATTTTCAACTCGTTTATTCTCAGT
ATTACGTTATGAAAGTGTTATTCATGAATTTGATCCATATTTTAATTATAGATCAACAAT
ATATCTTGTTCAAGAAGGTTTTTATAATTTTTTAAATTGGTTTGATGAAAGAGCATGGTA
TCCATTAGGACGTATTGTAGGTGGTACAATTTACCCAGGTTTAATGGCAACAGCAAGTTT
AGTTCATTGGTCATTGAATTCATTGAATATTACAGTTAATATTAGAAATGTATGTGTATT
GTTATCACCATGGTTTGCATCAAATACAGCAATGGTAACCTATAAATTTGCCAAXXXXXX
XXXXGGTCAAAATACTGCCGACGACGCTCGTATTATGTCTTGGTGGGATTATGGTTATCA
ATTATCTGCAATGGCCAATCGTACCGTATTGGTTGATAATAACACTTGGAACAATAGTCA
TATCGCTCAAGTTGGTAAAGCATTTGCATCCACTGAAGAAGATGCTTACATACAAATGAA
AGCATTGGGTGTCGATTATGTTTTAGTTATTTTTGGTGGTTTAACTGGTTACAGTTCTGA
TGATATCAATAAATTCCTTTGGATGGTTAGAATTGGTGGTAGTTGTGATCCAAATATTAA
AGAACAAGATTATCTCACCAATGGTCAATATAGAATAGATAAAGGTGCCTCACCAACAAT
GTTAAATTCTCTCATGTACAAACTTAGTTACTATCGTTTCTCTGAAGTTCACACTGACTA
TCAAAGACCAACAGGTTTCGATCGTGTAAGAAATGTTGAAATTGGTAATAAAAATTTCGA
TTTAACTTATTTAGAAGAAGCTTTCACATCTGTTCATTGGTTAGTTAGAGTTTATAAAGT
TAAAGATTT
sequence update 2002.10.25
Translated Amino Acid sequence 
ifidfvilvinivciciiivyisv*YISIFKQINNKKKMKRSEKSSTSVVSNNKQQDVNI
ISSNEVGVKEENKGHQEFLLKVLILSVIYVLAFSTRLFSVLRYESVIHEFDPYFNYRSTI
YLVQEGFYNFLNWFDERAWYPLGRIVGGTIYPGLMATASLVHWSLNSLNITVNIRNVCVL
LSPWFASNTAMVTYKFA---

---GQNTADDARIMSWWDYGYQLSAMANRTVLVDNNTWNNSHIAQVGKAFASTEEDAYIQ
MKALGVDYVLVIFGGLTGYSSDDINKFLWMVRIGGSCDPNIKEQDYLTNGQYRIDKGASP
TMLNSLMYKLSYYRFSEVHTDYQRPTGFDRVRNVEIGNKNFDLTYLEEAFTSVHWLVRVY
KVKD
Translated Amino Acid sequence (All Frames) 
Frame A:
nfyrfcyfshqycvymynncvy*ciiyiyf*tdqq*eknekirkikyicc***qttrcky
hqfk*swc*rrk*rtsriliksfnsishlcfsifnsfilsitl*kcys*i*sif*l*inn
iscsrrfl*ffklv**ksmvsirtycrwynlprfngnskfsslviefieyys*y*kcmci
vitmvcikysngnl*icq---

---GQNTADDARIMSWWDYGYQLSAMANRTVLVDNNTWNNSHIAQVGKAFASTEEDAYIQ
MKALGVDYVLVIFGGLTGYSSDDINKFLWMVRIGGSCDPNIKEQDYLTNGQYRIDKGASP
TMLNSLMYKLSYYRFSEVHTDYQRPTGFDRVRNVEIGNKNFDLTYLEEAFTSVHWLVRVY
KVKD

Frame B:
ifidfvilvinivciciiivyisv*YISIFKQINNKKKMKRSEKSSTSVVSNNKQQDVNI
ISSNEVGVKEENKGHQEFLLKVLILSVIYVLAFSTRLFSVLRYESVIHEFDPYFNYRSTI
YLVQEGFYNFLNWFDERAWYPLGRIVGGTIYPGLMATASLVHWSLNSLNITVNIRNVCVL
LSPWFASNTAMVTYKFA---

---vkilpttlvlclggimvinylqwpivpywliitlgtivislklvkhlhplkkmltyk
*khwvsimf*lflvv*lvtvlmisinsfgwlelvvvviqilknkiispmvnie*ikvphq
qc*ilsctnlvtivslkftltikdqqvsiv*emlklvikisi*li*kklshlfig*lefi
klki

Frame C:
fl*illf*ssilcvyv**lcilvyniylflnrstirkk*kdqknqvhlllvitnnkm*is
svqmklvlkkkikdiknsy*kf*fyqsfmf*hfqlvysqyyvmkvlfmnlihiliidqqy
ilfkkvfiif*iglmkehgih*dvl*vvqftqv*wqqqv*figh*ih*ilqlilemyvyc
yhhglhqiqqw*pinlp---

---skycrrrsyyvlvglwlsiicngqsyrig***hleq*syrssw*sicih*rrclhtn
esigcrlcfsyfwwfnwlqf**yq*ipldg*nww*l*sky*rtrlshqwsi*nr*rcltn
nvkfshvqt*llsfl*ssh*lsktnrfrsckkc*nw**kfrfnlfrrsfhicslvs*sl*
s*rf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE353 (VHE353Q) /CSM/VH/VHE3-C/VHE353Q.Seq.d/                       2012   0.0
VHQ247 (VHQ247Q) /CSM/VH/VHQ2-B/VHQ247Q.Seq.d/                       1072   0.0
VHQ183 (VHQ183Q) /CSM/VH/VHQ1-D/VHQ183Q.Seq.d/                       1072   0.0
VHP856 (VHP856Q) /CSM/VH/VHP8-C/VHP856Q.Seq.d/                       1072   0.0
VHO701 (VHO701Q) /CSM/VH/VHO7-A/VHO701Q.Seq.d/                       1072   0.0
VHO319 (VHO319Q) /CSM/VH/VHO3-A/VHO319Q.Seq.d/                       1072   0.0
VHN813 (VHN813Q) /CSM/VH/VHN8-A/VHN813Q.Seq.d/                       1072   0.0
VHK331 (VHK331Q) /CSM/VH/VHK3-B/VHK331Q.Seq.d/                       1072   0.0
VHJ693 (VHJ693Q) /CSM/VH/VHJ6-D/VHJ693Q.Seq.d/                       1072   0.0
VHJ440 (VHJ440Q) /CSM/VH/VHJ4-B/VHJ440Q.Seq.d/                       1072   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CS494261|CS494261.1 Sequence 103 from Patent WO2007006911.         94   2e-33  6
AX799082|AX799082.1 Sequence 1 from Patent WO03054008.             94   2e-33  6
AY074880|AY074880.1 Homo sapiens source of immunodominant MHC-associated peptides (SIMP) mRNA, complete cds.    94   2e-33  6
BU094108|BU094108.1 rf50d02.y2 Meloidogyne hapla J2 pAMP1 v1 Meloidogyne hapla cDNA 5' similar to SW:STT3_MOUSE P46978 OLIGOSACCHARYL TRANSFERASE STT3 SUBUNIT HOMOLOG ;, mRNA sequence.    80   1e-22  3
AM648058|AM648058.1 Entamoeba moshkovskii FIC GSS, clone mosh055c04.p1k.    90   1e-22  2
CV566713|CV566713.1 taj98f09.y1 Hydra EST UCI 6 Hydra magnipapillata cDNA 5' similar to SW:STT3_MOUSE P46978 OLIGOSACCHARYL TRANSFERASE STT3 SUBUNIT HOMOLOG ;, mRNA sequence.    64   1e-22  4
DT605445| Similar to intergral membrane protein 1 [Xenopus laevis], mRNA sequence.    64   1e-22  4
DN815438| Similar to intergral membrane protein 1 [Xenopus laevis], mRNA sequence.    64   1e-22  4
DN604052| Similar to intergral membrane protein 1 [Xenopus laevis], mRNA sequence.    64   1e-22  4
DT612703| Similar to intergral membrane protein 1 [Xenopus laevis], mRNA sequence.    64   1e-22  4
dna update 2007. 7.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BT064584_1(BT064584|pid:none) Zea mays full-length cDNA clone ZM...   277   6e-73
AC015446_5(AC015446|pid:none) Arabidopsis thaliana chromosome I ...   274   4e-72
AK229582_1(AK229582|pid:none) Arabidopsis thaliana mRNA for hypo...   274   4e-72
AK295892_1(AK295892|pid:none) Homo sapiens cDNA FLJ58575 complet...   271   3e-71
L47337_1(L47337|pid:none) Homo sapiens transmembrane protein (TM...   271   3e-71
CQ834708_1(CQ834708|pid:none) Sequence 579 from Patent WO2004058...   271   3e-71
AY891653_1(AY891653|pid:none) Synthetic construct Homo sapiens c...   271   3e-71
CQ834710_1(CQ834710|pid:none) Sequence 581 from Patent WO2004058...   271   3e-71
(P46977) RecName: Full=Dolichyl-diphosphooligosaccharide--protei...   271   3e-71
BC048348_1(BC048348|pid:none) Homo sapiens STT3, subunit of the ...   271   3e-71
protein update 2009. 6.30
PSORT 

 psg: 0.22  gvh: 0.21  alm: 0.22  top: 0.53  tms: 0.07  mit: 0.36  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: mitochondrial
	 28.0 %: nuclear
	 12.0 %: cytoplasmic
	 12.0 %: endoplasmic reticulum
	  8.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VHE353 is mit
5' end seq. ID VHE353F
5' end seq. 
>VHE353F.Seq
AATTTTTATAGATTTTGTTATTTTAGTCATCAATATTGTGTGTATATGTATAATAATTGT
GTATATTAGTGTATAATATATATCTATTTTTAAACAGATCAACAATAAGAAAAAAATGAA
AAGATCAGAAAAATCAAGTACATCTGTTGTTAGTAATAACAAACAACAAGATGTAAATAT
CATCAGTTCAAATGAAGTTGGTGTTAAAGAAGAAAATAAAGGACATCAAGAATTCTTATT
AAAAGTTTTAATTCTATCAGTCATTTATGTTTTAGCATTTTCAACTCGTTTATTCTCAGT
ATTACGTTATGAAAGTGTTATTCATGAATTTGATCCATATTTTAATTATAGATCAACAAT
ATATCTTGTTCAAGAAGGTTTTTATAATTTTTTAAATTGGTTTGATGAAAGAGCATGGTA
TCCATTAGGACGTATTGTAGGTGGTACAATTTACCCAGGTTTAATGGCAACAGCAAGTTT
AGTTCATTGGTCATTGAATTCATTGAATATTACAGTTAATATTAGAAATGTATGTGTATT
GTTATCACCATGGTTTGCATCAAATACAGCAATGGTAACCTATAAATTTGCCAANNNNNN
NNNN
Length of 5' end seq. 604
3' end seq. ID VHE353Z
3' end seq. 
>VHE353Z.Seq
NNNNNNNNNNGGTCAAAATACTGCCGACGACGCTCGTATTATGTCTTGGTGGGATTATGG
TTATCAATTATCTGCAATGGCCAATCGTACCGTATTGGTTGATAATAACACTTGGAACAA
TAGTCATATCGCTCAAGTTGGTAAAGCATTTGCATCCACTGAAGAAGATGCTTACATACA
AATGAAAGCATTGGGTGTCGATTATGTTTTAGTTATTTTTGGTGGTTTAACTGGTTACAG
TTCTGATGATATCAATAAATTCCTTTGGATGGTTAGAATTGGTGGTAGTTGTGATCCAAA
TATTAAAGAACAAGATTATCTCACCAATGGTCAATATAGAATAGATAAAGGTGCCTCACC
AACAATGTTAAATTCTCTCATGTACAAACTTAGTTACTATCGTTTCTCTGAAGTTCACAC
TGACTATCAAAGACCAACAGGTTTCGATCGTGTAAGAAATGTTGAAATTGGTAATAAAAA
TTTCGATTTAACTTATTTAGAAGAAGCTTTCACATCTGTTCATTGGTTAGTTAGAGTTTA
TAAAGTTAAAGATTT
Length of 3' end seq. 555
Connected seq. ID VHE353P
Connected seq. 
>VHE353P.Seq
AATTTTTATAGATTTTGTTATTTTAGTCATCAATATTGTGTGTATATGTATAATAATTGT
GTATATTAGTGTATAATATATATCTATTTTTAAACAGATCAACAATAAGAAAAAAATGAA
AAGATCAGAAAAATCAAGTACATCTGTTGTTAGTAATAACAAACAACAAGATGTAAATAT
CATCAGTTCAAATGAAGTTGGTGTTAAAGAAGAAAATAAAGGACATCAAGAATTCTTATT
AAAAGTTTTAATTCTATCAGTCATTTATGTTTTAGCATTTTCAACTCGTTTATTCTCAGT
ATTACGTTATGAAAGTGTTATTCATGAATTTGATCCATATTTTAATTATAGATCAACAAT
ATATCTTGTTCAAGAAGGTTTTTATAATTTTTTAAATTGGTTTGATGAAAGAGCATGGTA
TCCATTAGGACGTATTGTAGGTGGTACAATTTACCCAGGTTTAATGGCAACAGCAAGTTT
AGTTCATTGGTCATTGAATTCATTGAATATTACAGTTAATATTAGAAATGTATGTGTATT
GTTATCACCATGGTTTGCATCAAATACAGCAATGGTAACCTATAAATTTGCCAA------
----GGTCAAAATACTGCCGACGACGCTCGTATTATGTCTTGGTGGGATTATGGTTATCA
ATTATCTGCAATGGCCAATCGTACCGTATTGGTTGATAATAACACTTGGAACAATAGTCA
TATCGCTCAAGTTGGTAAAGCATTTGCATCCACTGAAGAAGATGCTTACATACAAATGAA
AGCATTGGGTGTCGATTATGTTTTAGTTATTTTTGGTGGTTTAACTGGTTACAGTTCTGA
TGATATCAATAAATTCCTTTGGATGGTTAGAATTGGTGGTAGTTGTGATCCAAATATTAA
AGAACAAGATTATCTCACCAATGGTCAATATAGAATAGATAAAGGTGCCTCACCAACAAT
GTTAAATTCTCTCATGTACAAACTTAGTTACTATCGTTTCTCTGAAGTTCACACTGACTA
TCAAAGACCAACAGGTTTCGATCGTGTAAGAAATGTTGAAATTGGTAATAAAAATTTCGA
TTTAACTTATTTAGAAGAAGCTTTCACATCTGTTCATTGGTTAGTTAGAGTTTATAAAGT
TAAAGATTT
Length of connected seq. 1139
Full length Seq ID -
Full length Seq. -
Length of full length seq. -