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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE311
Library VH
(Link to library)
Clone ID VHE311
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13616-1|Contig-U15923-1
Original site URL
Representative seq. ID VHE311P
(Link to Original site)
Representative DNA sequence 
>VHE311 (VHE311Q) /CSM/VH/VHE3-A/VHE311Q.Seq.d/
ATTTTTTTTAATTTGTATTAATCAATTTATTTATATATAAAATTAATAAAATGTCAGATT
CACAAGAAAATAAACCAGTTGAAACTCCAACTGAAGTTAAACCAGTTGCTGAAAAGAAAC
CAGCTGCTGAAAAGAAAGAAAAGAAACCAGCTGTAAAGAAAGTTGGTGTCATGGAAACTC
TCCAAACTCCAGATTACGTCAAAGAAAGAATTGAAATTTGGGAAAGTTTAAAAGCAAAAC
ACTTAGAATCACTTAAAGATATGAAAGAAGAACCAATTAATGTTACTTTACCAGATGGTA
AAGTTGTAGCAGGTATTAAAAATAAAACCACCCCATATGATATTGCAAAGGGTATTTCAA
GAGGTCTTGCTGATTCAATTGTTTCATCAAAAGTTAATGGTGAACAAATTTGGGATATCT
CTAGACCACTCGAAGCTGATTGTAATTTACAATTATGTAAATTTGATTCTGAAGAAGGTA
AAAAAACTTTTTGGCATTCATCTGCTCATATTTTAGGTCAAGCTATGGAAAGAATTTATG
GTGGTCAATTATGTATTGGTCCAGCCACTTCTGAAGGTTTTTATTATGATATGGCTATGG
GTGATAXXXXXXXXXXAATTTTAANAAAANNAATTTAAAATTTTTCCATTTTTTTTNTTT
TTTTTTTTTTACCAATCAATAATAATAATNAAAAAAAAAAATAAAATAAAATAAACATAA
TAATATTTTTA
sequence update 2002.10.25
Translated Amino Acid sequence 
ff*FVLINLFIYKINKMSDSQENKPVETPTEVKPVAEKKPAAEKKEKKPAVKKVGVMETL
QTPDYVKERIEIWESLKAKHLESLKDMKEEPINVTLPDGKVVAGIKNKTTPYDIAKGISR
GLADSIVSSKVNGEQIWDISRPLEADCNLQLCKFDSEEGKKTFWHSSAHILGQAMERIYG
GQLCIGPATSEGFYYDMAMGD---

---NFXKXNLKFFHFFXFFFFTNQ***xkkkik*nkhnnif
Translated Amino Acid sequence (All Frames) 
Frame A:
iffnly*siylyiklikcqihkkinqlklqlklnqllkrnqllkrkkrnql*rklvswkl
sklqitskkelkfgkv*kqnt*nhlki*kknqlmllyqmvkl*qvlkikpphmilqrvfq
evlliqlfhqklmvnkfgisldhskliviynyvnlilkkvkklfgihllif*vklwkefm
vvnyvlvqpllkvfimiwlwvi---

---NFXKXNLKFFHFFXFFFFTNQ***xkkkik*nkhnnif

Frame B:
fflicinqfiyi*n**nvrftrk*ts*nsn*s*tsc*ketsc*kerketsckeswchgns
pnsrlrqrkn*nlgkfksktlrit*ryerrtn*cyftrw*scsry*k*nhpi*yckgyfk
rsc*fncfiks*w*tnlgyl*ttrs*l*ftim*i*f*rr*knflaficsyfrssygknlw
wsimywsshf*rfll*ygyg*---

---ilxkxi*nfsiffxffflpinnnnxkkk*nkiniiifl

Frame C:
ff*FVLINLFIYKINKMSDSQENKPVETPTEVKPVAEKKPAAEKKEKKPAVKKVGVMETL
QTPDYVKERIEIWESLKAKHLESLKDMKEEPINVTLPDGKVVAGIKNKTTPYDIAKGISR
GLADSIVSSKVNGEQIWDISRPLEADCNLQLCKFDSEEGKKTFWHSSAHILGQAMERIYG
GQLCIGPATSEGFYYDMAMGD---

---f*xxxfkifpffxffffyqsiiixkkknkik*t**yf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE311 (VHE311Q) /CSM/VH/VHE3-A/VHE311Q.Seq.d/                        844   0.0
VHM231 (VHM231Q) /CSM/VH/VHM2-B/VHM231Q.Seq.d/                        839   0.0
CHL376 (CHL376Q) /CSM/CH/CHL3-D/CHL376Q.Seq.d/                        799   0.0
VHO874 (VHO874Q) /CSM/VH/VHO8-D/VHO874Q.Seq.d/                        757   0.0
VHL791 (VHL791Q) /CSM/VH/VHL7-D/VHL791Q.Seq.d/                        753   0.0
VHJ875 (VHJ875Q) /CSM/VH/VHJ8-D/VHJ875Q.Seq.d/                        751   0.0
VHI368 (VHI368Q) /CSM/VH/VHI3-C/VHI368Q.Seq.d/                        749   0.0
VHN251 (VHN251Q) /CSM/VH/VHN2-C/VHN251Q.Seq.d/                        747   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CD190771|CD190771.1 MS1-0064U-A312-B03-U.B MS1-0064 Schistosoma mansoni cDNA cloneMS1-0064U-A312-B03.B, mRNA sequence.    40   2e-04  3
EK392783|EK392783.1 1095469508239 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061005876386 3', genomic survey sequence.    52   0.001  2
DY228582|DY228582.1 ID0AAK4YI11CM1 ApHL3SD Acyrthosiphon pisum cDNA clone ID0AAK4YI11 5', mRNA sequence.    56   0.001  1
CN763282|CN763282.1 ID0AAA6CE03RM1 ApMS Acyrthosiphon pisum cDNA clone ID0AAA6CE03 5', mRNA sequence.    56   0.001  1
CN760781|CN760781.1 ID0AAA29BH10RM1 ApMS Acyrthosiphon pisum cDNA clone ID0AAA29BH10 5', mRNA sequence.    56   0.001  1
CN586696|CN586696.1 USDA-FP_129768 Acyrthosiphon pisum, Pea Aphid Acyrthosiphon pisum cDNA clone WHAP088_G02, mRNA sequence.    56   0.001  1
CN585146|CN585146.1 USDA-FP_128216 Acyrthosiphon pisum, Pea Aphid Acyrthosiphon pisum cDNA clone WHAP068_H04, mRNA sequence.    56   0.001  1
CN582483|CN582483.1 USDA-FP_125546 Acyrthosiphon pisum, Pea Aphid Acyrthosiphon pisum cDNA clone WHAP018_D03, mRNA sequence.    56   0.001  1
AE017245|AE017245.1 Mycoplasma synoviae 53, complete genome.       54   0.006  1
AM042926|AM042926.1 Schistosoma mansoni lung schistosomulum EST, clone SmlC50f09.q1k.    34   0.014  3
dna update 2007. 7.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54J66) RecName: Full=Probable threonyl-tRNA synthetase 1, cyto...   322   8e-87
AY119534_1(AY119534|pid:none) Drosophila melanogaster GH20022 fu...   169   1e-40
BT001848_1(BT001848|pid:none) Drosophila melanogaster RH56418 fu...   169   1e-40
AE014134_2248(AE014134|pid:none) Drosophila melanogaster chromos...   169   1e-40
BC154371_1(BC154371|pid:none) Danio rerio zgc:92586, mRNA (cDNA ...   161   2e-38
BX950187_1(BX950187|pid:none) Zebrafish DNA sequence from clone ...   161   2e-38
BC067949_1(BC067949|pid:none) Xenopus tropicalis threonyl-tRNA s...   160   3e-38
Z69902_11(Z69902|pid:none) Caenorhabditis elegans Cosmid C47D12,...   159   1e-37
(P52709) RecName: Full=Threonyl-tRNA synthetase, cytoplasmic;   ...   159   1e-37
(P26639) RecName: Full=Threonyl-tRNA synthetase, cytoplasmic;   ...   157   3e-37
protein update 2009. 6.30
PSORT 

 psg: 0.56  gvh: 0.28  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VHE311 is cyt
5' end seq. ID VHE311F
5' end seq. 
>VHE311F.Seq
ATTTTTTTTAATTTGTATTAATCAATTTATTTATATATAAAATTAATAAAATGTCAGATT
CACAAGAAAATAAACCAGTTGAAACTCCAACTGAAGTTAAACCAGTTGCTGAAAAGAAAC
CAGCTGCTGAAAAGAAAGAAAAGAAACCAGCTGTAAAGAAAGTTGGTGTCATGGAAACTC
TCCAAACTCCAGATTACGTCAAAGAAAGAATTGAAATTTGGGAAAGTTTAAAAGCAAAAC
ACTTAGAATCACTTAAAGATATGAAAGAAGAACCAATTAATGTTACTTTACCAGATGGTA
AAGTTGTAGCAGGTATTAAAAATAAAACCACCCCATATGATATTGCAAAGGGTATTTCAA
GAGGTCTTGCTGATTCAATTGTTTCATCAAAAGTTAATGGTGAACAAATTTGGGATATCT
CTAGACCACTCGAAGCTGATTGTAATTTACAATTATGTAAATTTGATTCTGAAGAAGGTA
AAAAAACTTTTTGGCATTCATCTGCTCATATTTTAGGTCAAGCTATGGAAAGAATTTATG
GTGGTCAATTATGTATTGGTCCAGCCACTTCTGAAGGTTTTTATTATGATATGGCTATGG
GTGATANNNNNNNNNN
Length of 5' end seq. 616
3' end seq. ID VHE311Z
3' end seq. 
>VHE311Z.Seq
NNNNNNNNNNAATTTTAANAAAANNAATTTAAAATTTTTCCATTTTTTTTNTTTTTTTTT
TTTTACCAATCAATAATAATAATNAAAAAAAAAAATAAAATAAAATAAACATAATAATAT
TTTTA
Length of 3' end seq. 125
Connected seq. ID VHE311P
Connected seq. 
>VHE311P.Seq
ATTTTTTTTAATTTGTATTAATCAATTTATTTATATATAAAATTAATAAAATGTCAGATT
CACAAGAAAATAAACCAGTTGAAACTCCAACTGAAGTTAAACCAGTTGCTGAAAAGAAAC
CAGCTGCTGAAAAGAAAGAAAAGAAACCAGCTGTAAAGAAAGTTGGTGTCATGGAAACTC
TCCAAACTCCAGATTACGTCAAAGAAAGAATTGAAATTTGGGAAAGTTTAAAAGCAAAAC
ACTTAGAATCACTTAAAGATATGAAAGAAGAACCAATTAATGTTACTTTACCAGATGGTA
AAGTTGTAGCAGGTATTAAAAATAAAACCACCCCATATGATATTGCAAAGGGTATTTCAA
GAGGTCTTGCTGATTCAATTGTTTCATCAAAAGTTAATGGTGAACAAATTTGGGATATCT
CTAGACCACTCGAAGCTGATTGTAATTTACAATTATGTAAATTTGATTCTGAAGAAGGTA
AAAAAACTTTTTGGCATTCATCTGCTCATATTTTAGGTCAAGCTATGGAAAGAATTTATG
GTGGTCAATTATGTATTGGTCCAGCCACTTCTGAAGGTTTTTATTATGATATGGCTATGG
GTGATA----------AATTTTAANAAAANNAATTTAAAATTTTTCCATTTTTTTTNTTT
TTTTTTTTTTACCAATCAATAATAATAATNAAAAAAAAAAATAAAATAAAATAAACATAA
TAATATTTTTA
Length of connected seq. 721
Full length Seq ID -
Full length Seq. -
Length of full length seq. -