NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE310
Library VH
(Link to library)
Clone ID VHE310
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15307-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VHE310 (VHE310Q) /CSM/VH/VHE3-A/VHE310Q.Seq.d/
XXXXXXXXXXCTTTTCAAAGTTTCAGGTGCCACCTTTTTGGTATTCTCAGACTATCTTCG
TCCAGCCATCCGTTTGGCTGCTCTCTCACATTTACCAGTAGTTTACATTTTCACTCATGA
CTCTGTTGGTGTTGGTGAAGATGGTCCAACTCATCAACCAGTTGAAACCGTTTCAGGTCT
TCGTATGATTCCAAACTTGGATGTCATTCGTCCAGCCGATCCAGAAGAAACAGCTGCTGC
TTTCTCTTTGGCTTATGCTCGTGCCGATGGTCCAACCCTTTTATCATTAACTCGTCAAAA
TCTTCCATTCCTCCCAGGTACCGCTCAAAAGAAGAGAGAAGGTACTCTCAGAGGTGGTTA
CATTGTCGTTAGTGAAACCGCTCCATTAAGAATGATCTTAATTGCCACTGGTTCAGAAGT
TCAACATTGTGTTGAAGCCGCTAAATTATTAGGTGATGATATTCGTGTCGTCTCTATGCC
ATGCACTGAACTTTTCGATCGTCAATCAAATGAATACAAACAATCCGTACTTCCAAGTGG
TTGTAGAAATCGTATCGCTATGGAAGCTGGTGTCACTTCATTCTGGTATAAATATGTTGG
TCTTGATGGTAAAGTCATTGGTATTGATAGATTTGGTATGTCTGCACCAGGTAATGCCGT
TAGAAACAATTAGGTAGACTTCNAAAATTTAGTAAACATTTCAAAACA
sequence update 2002. 9.10
Translated Amino Acid sequence 
---FKVSGATFLVFSDYLRPAIRLAALSHLPVVYIFTHDSVGVGEDGPTHQPVETVSGLR
MIPNLDVIRPADPEETAAAFSLAYARADGPTLLSLTRQNLPFLPGTAQKKREGTLRGGYI
VVSETAPLRMILIATGSEVQHCVEAAKLLGDDIRVVSMPCTELFDRQSNEYKQSVLPSGC
RNRIAMEAGVTSFWYKYVGLDGKVIGIDRFGMSAPGNAVRNN*vdfxnlvnisk
Translated Amino Acid sequence (All Frames) 
Frame A:
---fskfqvppfwysqtifvqpsvwllshiyq*ftfslmtllvlvkmvqlinqlkpfqvf
v*fqtwmsfvqpiqkkqlllslwlmlvpmvqpfyh*lvkifhssqvplkrrekvlsevvt
lslvkplh*e*s*lplvqkfnivlkplny*vmifvsslchalnfsivnqmntnnpyfqvv
veivslwklvslhsginmlvlmvkslvlidlvclhqvmpletir*tski**tfqn


Frame B:
---fqsfrchlfgilrlsssshpfgcsltftsslhfhs*lcwcw*rwsnssts*nrfrss
ydsklgchsssrsrrnsccflfglcscrwsnpfiinsskssippryrskeerrysqrwlh
cr**nrsikndlnchwfrsstlc*sr*iir**yscrlyamh*tfrssik*iqtirtskwl
*ksyrygswchfilv*icws*w*shwy**iwyvctr*cr*kqlgrlxkfskhfkt


Frame C:
---FKVSGATFLVFSDYLRPAIRLAALSHLPVVYIFTHDSVGVGEDGPTHQPVETVSGLR
MIPNLDVIRPADPEETAAAFSLAYARADGPTLLSLTRQNLPFLPGTAQKKREGTLRGGYI
VVSETAPLRMILIATGSEVQHCVEAAKLLGDDIRVVSMPCTELFDRQSNEYKQSVLPSGC
RNRIAMEAGVTSFWYKYVGLDGKVIGIDRFGMSAPGNAVRNN*vdfxnlvnisk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP323 (VHP323Q) /CSM/VH/VHP3-A/VHP323Q.Seq.d/                       1378   0.0
VHE310 (VHE310Q) /CSM/VH/VHE3-A/VHE310Q.Seq.d/                       1378   0.0
SFF764 (SFF764Q) /CSM/SF/SFF7-C/SFF764Q.Seq.d/                       1364   0.0
CHQ579 (CHQ579Q) /CSM/CH/CHQ5-D/CHQ579Q.Seq.d/                       1364   0.0
CHM832 (CHM832Q) /CSM/CH/CHM8-B/CHM832Q.Seq.d/                       1364   0.0
AFN107 (AFN107Q) /CSM/AF/AFN1-A/AFN107Q.Seq.d/                       1364   0.0
AFF302 (AFF302Q) /CSM/AF/AFF3-A/AFF302Q.Seq.d/                       1364   0.0
AFJ520 (AFJ520Q) /CSM/AF/AFJ5-A/AFJ520Q.Seq.d/                       1358   0.0
AFH190 (AFH190Q) /CSM/AF/AFH1-D/AFH190Q.Seq.d/                       1358   0.0
SFH288 (SFH288Q) /CSM/SF/SFH2-D/SFH288Q.Seq.d/                       1350   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.  1292   0.0    3
ER462265|ER462265.1 1092963900917 Global-Ocean-Sampling_GS-35-01-01-1P5KB marine metagenome genomic clone 1061002399063 5', genomic survey sequence.    84   6e-12  1
EF216683|EF216683.1 Karlodinium micrum transketolase mRNA, partial cds.    58   6e-12  3
BQ540728|BQ540728.1 PTAM1055 Phaeodactylum tricornutum Uni-Zap XR Phaeodactylum tricornutum cDNA 5', mRNA sequence.    68   3e-11  2
AE006393|AE006393.1 Lactococcus lactis subsp. lactis IL1403 section 155 of 218 of the complete genome.    42   8e-11  5
CD384397|CD384397.1 PTMM09570 Phaeodactylum tricornutum Uni-Zap XR Phaeodactylum tricornutum cDNA 5', mRNA sequence.    68   5e-10  2
CD379071|CD379071.1 PTMM04240 Phaeodactylum tricornutum Uni-Zap XR Phaeodactylum tricornutum cDNA 5', mRNA sequence.    68   5e-10  2
EK303511|EK303511.1 1095462372648 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061005791520 5', genomic survey sequence.    76   2e-09  1
EK248242|EK248242.1 1095460267104 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061005791520 3', genomic survey sequence.    76   2e-09  1
CD380856|CD380856.1 PTMM06025 Phaeodactylum tricornutum Uni-Zap XR Phaeodactylum tricornutum cDNA 5', mRNA sequence.    68   3e-08  2
dna update 2007. 7.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q556J0) RecName: Full=Transketolase;          Short=TK;        ...   438   e-122
(A6YB01) RecName: Full=Transketolase;          Short=TK;        ...   299   6e-80
AM746676_3991(AM746676|pid:none) Sorangium cellulosum 'So ce 56'...   296   5e-79
CP001032_3788(CP001032|pid:none) Opitutus terrae PB90-1, complet...   294   2e-78
EF216683_1(EF216683|pid:none) Karlodinium micrum transketolase m...   283   5e-75
AJ821796_1(AJ821796|pid:none) Guillardia theta partial mRNA for ...   279   7e-74
EF216682_1(EF216682|pid:none) Karlodinium micrum transketolase m...   277   3e-73
EF216685_1(EF216685|pid:none) Bigelowiella natans transketolase ...   276   3e-73
EF216681_1(EF216681|pid:none) Isochrysis galbana putative plasti...   245   1e-63
AP009484_999(AP009484|pid:none) Macrococcus caseolyticus JCSC540...   242   7e-63
protein update 2009. 6.30
PSORT 

 psg: 0.93  gvh: 0.46  alm: 0.37  top: 0.50  tms: 0.00  mit: 0.30  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHE310 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID VHE310Z
3' end seq. 
>VHE310Z.Seq
NNNNNNNNNNCTTTTCAAAGTTTCAGGTGCCACCTTTTTGGTATTCTCAGACTATCTTCG
TCCAGCCATCCGTTTGGCTGCTCTCTCACATTTACCAGTAGTTTACATTTTCACTCATGA
CTCTGTTGGTGTTGGTGAAGATGGTCCAACTCATCAACCAGTTGAAACCGTTTCAGGTCT
TCGTATGATTCCAAACTTGGATGTCATTCGTCCAGCCGATCCAGAAGAAACAGCTGCTGC
TTTCTCTTTGGCTTATGCTCGTGCCGATGGTCCAACCCTTTTATCATTAACTCGTCAAAA
TCTTCCATTCCTCCCAGGTACCGCTCAAAAGAAGAGAGAAGGTACTCTCAGAGGTGGTTA
CATTGTCGTTAGTGAAACCGCTCCATTAAGAATGATCTTAATTGCCACTGGTTCAGAAGT
TCAACATTGTGTTGAAGCCGCTAAATTATTAGGTGATGATATTCGTGTCGTCTCTATGCC
ATGCACTGAACTTTTCGATCGTCAATCAAATGAATACAAACAATCCGTACTTCCAAGTGG
TTGTAGAAATCGTATCGCTATGGAAGCTGGTGTCACTTCATTCTGGTATAAATATGTTGG
TCTTGATGGTAAAGTCATTGGTATTGATAGATTTGGTATGTCTGCACCAGGTAATGCCGT
TAGAAACAATTAGGTAGACTTCNAAAATTTAGTAAACATTTCAAAACA
Length of 3' end seq. 708
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -