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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE271
Library VH
(Link to library)
Clone ID VHE271
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11249-1|Contig-U12842-1
Original site URL
Representative seq. ID VHE271P
(Link to Original site)
Representative DNA sequence 
>VHE271 (VHE271Q) /CSM/VH/VHE2-C/VHE271Q.Seq.d/
AAAATGCCCCAAAATAAAAGTCAAACAATAAAACTATCTCATTTTTTAAGAGATAATCTT
CTTAACTATCCAGAATCAAAAATATTTAGAGAATTGATTCAAAATGCAGAAGATGCTCGA
GCTGATACAGTCATAATAAAGGTTGATGAAGGCTCATATCCAAATAATGGGTTGTTAATT
TCAAATGATAACCAAGAAATTGAAAGTTCAAAAGAATTATTAGGTCCATCAATTTTAATT
TATAATAATGCAACTTTTCAAGAACATGATTGGGAAGGAATTTGTCAAATTAGTCAAGGT
AGTAAAAAAGAAAATTTAAAAAGTGTTGGCAATTTCGGTTTAGGTTGGAACAGTGTTTAT
CATATAACAGATAATCCAATTGTTTATAGTGGTAATAATGTTTGGTTCTCAGATCCAAAC
GAAAGAATGAGTGGTGGTTTATTCTTTGATTTAGATGACGAAGATGATTATAAGAAATGT
TTTAGTTTTTTAGAGCCATTCATTCAATTTAAAGAATCAAATTGTAACCCAAAAGAATAT
TTTAATGGTACAATCATTCGTCTACCATTGAGAATXXXXXXXXXXGAAAAAGAAGTTTAT
AGATTTTATTTAATTCAAAATATTAGTTCCTTTGAATTAAACCACTCAATTGAAATTATT
AAAAACTTTTTAACTCTTTATAGTAATCATTATACCAATGATAGTGAATTAAGAAAATTA
CAATTTATTCCATCTGAAAATGGTGTTTATTCAACTCCTGATTCATTTTATGTTTTAGAT
CCACTTGAAAAAGAATTAATTAGAAAAATCGAACCATTCAAGATTTTAAATTCATCTTTG
GATGTTTTTATTGGTTATTTAAGAATATTTGGATTGGTTAATAGACCTTCAGATAGTCAT
ATATGTTCCAAGATTTCAAGTTTACTTTCCAATCAAGATTCAATAGAAACTTATCAAACT
TTTATTAATTATTTAAATCAAAGAGGTGAGAATATTACTGTTTCAATAATTAATTCTTTC
AAAGATATTCCATTTATTCCACCCAGTGAATTTGATACCTATGACGGATTTGCTCCATAT
AAATATAAAATTTCATTATCAGAGTGCGCATTATCAAGTTATAAAGATTGTTTATTCACT
GTAAAAACTCCAATTAAAGTAATTTTCAATTACAACAACCCCATTTACAAATACCTATCA
TCAAATTTAAATGATAACATTATTCAGCATTTGATTAATTTAAATGCTATGGAATTCAAT
GATGAAACATCATTCAACCCCAATGAAGTTTTCGACAATACCTATAAAAGAATGATAGTT
NATTTTTACAAATTCCATACTAAATG
sequence update 2002.10.25
Translated Amino Acid sequence 
KMPQNKSQTIKLSHFLRDNLLNYPESKIFRELIQNAEDARADTVIIKVDEGSYPNNGLLI
SNDNQEIESSKELLGPSILIYNNATFQEHDWEGICQISQGSKKENLKSVGNFGLGWNSVY
HITDNPIVYSGNNVWFSDPNERMSGGLFFDLDDEDDYKKCFSFLEPFIQFKESNCNPKEY
FNGTIIRLPLR---

---EKEVYRFYLIQNISSFELNHSIEIIKNFLTLYSNHYTNDSELRKLQFIPSENGVYST
PDSFYVLDPLEKELIRKIEPFKILNSSLDVFIGYLRIFGLVNRPSDSHICSKISSLLSNQ
DSIETYQTFINYLNQRGENITVSIINSFKDIPFIPPSEFDTYDGFAPYKYKISLSECALS
SYKDCLFTVKTPIKVIFNYNNPIYKYLSSNLNDNIIQHLINLNAMEFNDETSFNPNEVFD
NTYKRMIVXFYKFHTK
Translated Amino Acid sequence (All Frames) 
Frame A:
KMPQNKSQTIKLSHFLRDNLLNYPESKIFRELIQNAEDARADTVIIKVDEGSYPNNGLLI
SNDNQEIESSKELLGPSILIYNNATFQEHDWEGICQISQGSKKENLKSVGNFGLGWNSVY
HITDNPIVYSGNNVWFSDPNERMSGGLFFDLDDEDDYKKCFSFLEPFIQFKESNCNPKEY
FNGTIIRLPLR---

---EKEVYRFYLIQNISSFELNHSIEIIKNFLTLYSNHYTNDSELRKLQFIPSENGVYST
PDSFYVLDPLEKELIRKIEPFKILNSSLDVFIGYLRIFGLVNRPSDSHICSKISSLLSNQ
DSIETYQTFINYLNQRGENITVSIINSFKDIPFIPPSEFDTYDGFAPYKYKISLSECALS
SYKDCLFTVKTPIKVIFNYNNPIYKYLSSNLNDNIIQHLINLNAMEFNDETSFNPNEVFD
NTYKRMIVXFYKFHTK

Frame B:
kcpkikvkq*nylif*eiifltiqnqkylen*fkmqkmleliqs**rlmkahiqimgc*f
qmitkklkvqkny*vhqf*fiimqlfknmigkefvklvkvvkkki*kvlaisv*vgtvfi
i*qiiqlfivvimfgsqiqtke*vvvysli*mtkmiirnvlvf*shsfnlknqivtqkni
lmvqsfvyh*e---

---kkkfidfi*fkilvpln*ttqlkllktf*lfiviiipmivn*enynlfhlkmvfiql
lihfmf*ihlkkn*leksnhsrf*ihlwmfllvi*eyldwlidlqiviyvprfqvyfpik
iq*kliklllii*ikevrillfq*lilskifhlfhpvnlipmtdllhinikfhyqsahyq
vikivysl*klqlk*fsitttpftntyhqi*mitlfsi*li*mlwnsmmkhhstpmkfst
ipike**xiftnsiln

Frame C:
napk*ksnnktisffkr*ss*lsrikni*ridskcrrcss*yshnkg**rlisk*wvvnf
k**prn*kfkriirsinfnl**cnfsrt*lgrnlsn*sr**krkfkkcwqfrfrleqcls
ynr*sncl*w**clvlrskrknewwfil*fr*rr*l*emf*ffraihsi*rikl*pkrif
*wynhsstien---

---krsl*ilfnsky*fl*ikpln*ny*klfnsl**slyq***ikkitiysi*kwclfns
*filcfrst*krin*knrtiqdfkfifgcfywlfkniwig**tfr*symfqdfkftfqsr
fnrnlsnfy*lfkskr*eyycfnn*ffqrysiystq*i*yl*ricsi*i*nfiirvriik
l*rlfihcknsn*snfqlqqphlqipiikfk**hysafd*fkcygiq**niiqpq*sfrq
yl*kndsxflqipy*m
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE271 (VHE271Q) /CSM/VH/VHE2-C/VHE271Q.Seq.d/                       1965   0.0
AHQ220 (AHQ220Q) /CSM/AH/AHQ2-A/AHQ220Q.Seq.d/                       1413   0.0
VHN647 (VHN647Q) /CSM/VH/VHN6-B/VHN647Q.Seq.d/                       1376   0.0
VHC102 (VHC102Q) /CSM/VH/VHC1-A/VHC102Q.Seq.d/                       1168   0.0
VHK810 (VHK810Q) /CSM/VH/VHK8-A/VHK810Q.Seq.d/                        601   e-170
VHD239 (VHD239Q) /CSM/VH/VHD2-B/VHD239Q.Seq.d/                        601   e-170
VHB767 (VHB767Q) /CSM/VH/VHB7-C/VHB767Q.Seq.d/                        601   e-170
VHO809 (VHO809Q) /CSM/VH/VHO8-A/VHO809Q.Seq.d/                        593   e-168
VHE123 (VHE123Q) /CSM/VH/VHE1-A/VHE123Q.Seq.d/                        593   e-168
VHK791 (VHK791Q) /CSM/VH/VHK7-D/VHK791Q.Seq.d/                        581   e-164
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BA000026|BA000026.2 Mycoplasma penetrans HF-2 DNA, complete genome.    32   8e-05  20
BA000021|BA000021.3 Wigglesworthia glossinidia endosymbiont of Glossina brevipalpis DNA, complete genome.    36   0.002  16
AC005308|AC005308.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    36   0.003  12
BX293980|BX293980.2 Mycoplasma mycoides subsp. mycoides SC str. PG1, complete genome.    42   0.004  19
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    36   0.007  18
CP000413|CP000413.1 Lactobacillus gasseri ATCC 33323, complete genome.    42   0.013  26
AX344551|AX344551.1 Sequence 2 from Patent WO0200932.              34   0.014  13
AM180355|AM180355.1 Clostridium difficile 630 complete genome.     36   0.016  20
BX005112|BX005112.19 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    38   0.019  8
AC115599|AC115599.2 Dictyostelium discoideum chromosome 2 map 4229098-4354721 strain AX4, complete sequence.    38   0.023  10
dna update 2007. 7.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC142916_1(BC142916|pid:none) Danio rerio hypothetical LOC555303...   111   7e-23
CP001577_292(CP001577|pid:none) Micromonas sp. RCC299 chromosome...   105   3e-21
(Q9NZJ4) RecName: Full=Sacsin;  &AL157766_4(AL157766|pid:none)        105   3e-21
BC171956_1(BC171956|pid:none) Mus musculus sacsin, mRNA (cDNA cl...   103   1e-20
BC138482_1(BC138482|pid:none) Mus musculus sacsin, mRNA (cDNA cl...   103   1e-20
(Q9JLC8) RecName: Full=Sacsin;                                        103   1e-20
AB006708_10(AB006708|pid:none) Arabidopsis thaliana genomic DNA,...   100   9e-20
AL732351_6(AL732351|pid:none) Oryza sativa genomic DNA, chromoso...    92   6e-17
AL606457_18(AL606457|pid:none) Oryza sativa genomic DNA, chromos...    90   2e-16
CR749427_1(CR749427|pid:none) Homo sapiens mRNA; cDNA DKFZp686B1...    76   3e-12
protein update 2009. 6.30
PSORT 

 psg: 0.55  gvh: 0.39  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.37  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: mitochondrial
	 12.0 %: nuclear
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VHE271 is cyt
5' end seq. ID VHE271F
5' end seq. 
>VHE271F.Seq
AAAATGCCCCAAAATAAAAGTCAAACAATAAAACTATCTCATTTTTTAAGAGATAATCTT
CTTAACTATCCAGAATCAAAAATATTTAGAGAATTGATTCAAAATGCAGAAGATGCTCGA
GCTGATACAGTCATAATAAAGGTTGATGAAGGCTCATATCCAAATAATGGGTTGTTAATT
TCAAATGATAACCAAGAAATTGAAAGTTCAAAAGAATTATTAGGTCCATCAATTTTAATT
TATAATAATGCAACTTTTCAAGAACATGATTGGGAAGGAATTTGTCAAATTAGTCAAGGT
AGTAAAAAAGAAAATTTAAAAAGTGTTGGCAATTTCGGTTTAGGTTGGAACAGTGTTTAT
CATATAACAGATAATCCAATTGTTTATAGTGGTAATAATGTTTGGTTCTCAGATCCAAAC
GAAAGAATGAGTGGTGGTTTATTCTTTGATTTAGATGACGAAGATGATTATAAGAAATGT
TTTAGTTTTTTAGAGCCATTCATTCAATTTAAAGAATCAAATTGTAACCCAAAAGAATAT
TTTAATGGTACAATCATTCGTCTACCATTGAGAATNNNNNNNNNN
Length of 5' end seq. 585
3' end seq. ID VHE271Z
3' end seq. 
>VHE271Z.Seq
NNNNNNNNNNGAAAAAGAAGTTTATAGATTTTATTTAATTCAAAATATTAGTTCCTTTGA
ATTAAACCACTCAATTGAAATTATTAAAAACTTTTTAACTCTTTATAGTAATCATTATAC
CAATGATAGTGAATTAAGAAAATTACAATTTATTCCATCTGAAAATGGTGTTTATTCAAC
TCCTGATTCATTTTATGTTTTAGATCCACTTGAAAAAGAATTAATTAGAAAAATCGAACC
ATTCAAGATTTTAAATTCATCTTTGGATGTTTTTATTGGTTATTTAAGAATATTTGGATT
GGTTAATAGACCTTCAGATAGTCATATATGTTCCAAGATTTCAAGTTTACTTTCCAATCA
AGATTCAATAGAAACTTATCAAACTTTTATTAATTATTTAAATCAAAGAGGTGAGAATAT
TACTGTTTCAATAATTAATTCTTTCAAAGATATTCCATTTATTCCACCCAGTGAATTTGA
TACCTATGACGGATTTGCTCCATATAAATATAAAATTTCATTATCAGAGTGCGCATTATC
AAGTTATAAAGATTGTTTATTCACTGTAAAAACTCCAATTAAAGTAATTTTCAATTACAA
CAACCCCATTTACAAATACCTATCATCAAATTTAAATGATAACATTATTCAGCATTTGAT
TAATTTAAATGCTATGGAATTCAATGATGAAACATCATTCAACCCCAATGAAGTTTTCGA
CAATACCTATAAAAGAATGATAGTTNATTTTTACAAATTCCATACTAAATG
Length of 3' end seq. 771
Connected seq. ID VHE271P
Connected seq. 
>VHE271P.Seq
AAAATGCCCCAAAATAAAAGTCAAACAATAAAACTATCTCATTTTTTAAGAGATAATCTT
CTTAACTATCCAGAATCAAAAATATTTAGAGAATTGATTCAAAATGCAGAAGATGCTCGA
GCTGATACAGTCATAATAAAGGTTGATGAAGGCTCATATCCAAATAATGGGTTGTTAATT
TCAAATGATAACCAAGAAATTGAAAGTTCAAAAGAATTATTAGGTCCATCAATTTTAATT
TATAATAATGCAACTTTTCAAGAACATGATTGGGAAGGAATTTGTCAAATTAGTCAAGGT
AGTAAAAAAGAAAATTTAAAAAGTGTTGGCAATTTCGGTTTAGGTTGGAACAGTGTTTAT
CATATAACAGATAATCCAATTGTTTATAGTGGTAATAATGTTTGGTTCTCAGATCCAAAC
GAAAGAATGAGTGGTGGTTTATTCTTTGATTTAGATGACGAAGATGATTATAAGAAATGT
TTTAGTTTTTTAGAGCCATTCATTCAATTTAAAGAATCAAATTGTAACCCAAAAGAATAT
TTTAATGGTACAATCATTCGTCTACCATTGAGAAT----------GAAAAAGAAGTTTAT
AGATTTTATTTAATTCAAAATATTAGTTCCTTTGAATTAAACCACTCAATTGAAATTATT
AAAAACTTTTTAACTCTTTATAGTAATCATTATACCAATGATAGTGAATTAAGAAAATTA
CAATTTATTCCATCTGAAAATGGTGTTTATTCAACTCCTGATTCATTTTATGTTTTAGAT
CCACTTGAAAAAGAATTAATTAGAAAAATCGAACCATTCAAGATTTTAAATTCATCTTTG
GATGTTTTTATTGGTTATTTAAGAATATTTGGATTGGTTAATAGACCTTCAGATAGTCAT
ATATGTTCCAAGATTTCAAGTTTACTTTCCAATCAAGATTCAATAGAAACTTATCAAACT
TTTATTAATTATTTAAATCAAAGAGGTGAGAATATTACTGTTTCAATAATTAATTCTTTC
AAAGATATTCCATTTATTCCACCCAGTGAATTTGATACCTATGACGGATTTGCTCCATAT
AAATATAAAATTTCATTATCAGAGTGCGCATTATCAAGTTATAAAGATTGTTTATTCACT
GTAAAAACTCCAATTAAAGTAATTTTCAATTACAACAACCCCATTTACAAATACCTATCA
TCAAATTTAAATGATAACATTATTCAGCATTTGATTAATTTAAATGCTATGGAATTCAAT
GATGAAACATCATTCAACCCCAATGAAGTTTTCGACAATACCTATAAAAGAATGATAGTT
NATTTTTACAAATTCCATACTAAATG
Length of connected seq. 1336
Full length Seq ID -
Full length Seq. -
Length of full length seq. -