>VHE264 (VHE264Q) /CSM/VH/VHE2-C/VHE264Q.Seq.d/
AAAAGTACACCAGAGAATCAAGTTTTCACATCAGTACTACGTGCAATGTATTGTACTCCA
GATGGTTTCAACTTTGAGCAAGGTGATGACCCAATTCAAGATGACCCAAATTTATTCGAT
AATAACGTAGACTCAAGAGCTGAACAGTTCACTCAAGTGGCACTTGAATATGCAACTCAT
TATCGTACCGATAATGTACTAATTCCATTTGGTTGTGATTTCGCTTACCTTAACGCTCAA
ATGTATTACAAAAACATTGACAAACTAATCGCACATATCAATTCAAATCCGGATAAATAT
GGTTTAAATTTGTTATATTCAACTCCATCAATTTATATAGATGCTGTAAATGATGCCAAT
CTAGTATGGGAAGTGAAAACTGATGATCTCTTCCCATATGCTGATAATGAATTTAGTTAT
TGGACTGGTTATTTCGTTAGTCGTCCAGCATTGAAAGGCTACGTTCGTCAAAATAATGCT
CTATTACATGTAGTTGAACAAATGTTGGTAACTAGTAGTAATCTTATGCCAAGTAGTAGA
TCTGAACAATTGGTTGATGACATTGTTATAATGAGAGAGGTAATGGGTATTGCTCAACAT
CATGATGCCGTATCAXXXXXXXXXXTCCATCATGGAATTTANCCGTGGTTCAACCAACCA
GTGGTAATTATGTACCTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAATCATTAC
AATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGG
ATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGA
ATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTATG
CTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATGTTTA
CAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCACCAT
TGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATA
ATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGATT
CACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAATCA
CTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAA
AATGGAAAACTGTTGATGGTAAAAACTATGATCATAAATCATCATCATCAACTAAAGAAG
ATTCTTCAAAGGTTTTGTTTTTACTTTTTCTCCAATGCAAATCAGAACTTTTATAATTAC
TAC

KSTPENQVFTSVLRAMYCTPDGFNFEQGDDPIQDDPNLFDNNVDSRAEQFTQVALEYATH
YRTDNVLIPFGCDFAYLNAQMYYKNIDKLIAHINSNPDKYGLNLLYSTPSIYIDAVNDAN
LVWEVKTDDLFPYADNEFSYWTGYFVSRPALKGYVRQNNALLHVVEQMLVTSSNLMPSSR
SEQLVDDIVIMREVMGIAQHHDAVS---

---PSWNLXVVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHR
RTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQS
SNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQT
ITLDLSTIFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKEDSSKVL
FLLFLQCKSELL*ll

Frame A:
KSTPENQVFTSVLRAMYCTPDGFNFEQGDDPIQDDPNLFDNNVDSRAEQFTQVALEYATH
YRTDNVLIPFGCDFAYLNAQMYYKNIDKLIAHINSNPDKYGLNLLYSTPSIYIDAVNDAN
LVWEVKTDDLFPYADNEFSYWTGYFVSRPALKGYVRQNNALLHVVEQMLVTSSNLMPSSR
SEQLVDDIVIMREVMGIAQHHDAVS---

---simefxrgstnqw*lctcecyclhsrs*siitihhch**kswmcivkrwsigyddas
syfer*wswcwstne*vnsnryn**idfp*yfklcsislsscctlfitsitpnvynhsti
fkrlefsisrclltidfsltftkwfkdsnstmvr*sr*yhtfty*kylsn*wsrftrstn
yhfrsfnylfnnynhfcn*nefnwcskiikfikikmenc*w*kl*s*iiiin*rrffkgf
vftfspmqirtfiit

Frame B:
kvhqrikfshqyyvqcivlqmvstlskvmtqfkmtqiysiit*tqelnsslkwhlnmqli
ivpimy*fhlvvisltltlkcitktltn*shisiqirinmv*icyiqlhqfi*ml*mmpi
*ygk*klmisshmlimnlviglvislvvqh*katfvkimlyym*lnkcw*lvvilcqvvd
lnnwlmtll**er*wvllnimmpy---

---PSWNLXVVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHR
RTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQS
SNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQT
ITLDLSTIFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKEDSSKVL
FLLFLQCKSELL*ll

Frame C:
kytressfhisttcnvlysrwfql*ar**pnsr*pkfir**rrlks*tvhssgt*icnsl
syr*ctnsiwl*frlp*rsnvlqkh*qtnrtyqfksg*iwfkfvifnsinlyrcck*cqs
smgsen**slpic***i*lldwlfr*sssierlrssk*csitcs*tnvgn***syak**i
*tig**hcynergngycsts*cri---

---hhgixpwfnqpvvimyl*mllptfkilinhynsplslievvdvhr*emvnwi**civ
vl*kmmvvvlvnq*msqlkslqlvn*fsmifqvmlnliivllhslyhihysqclqplnnl
qtigilnikvfthh*lqphlyqmv*rfklyng*iikiipyfyvlkifiklmvkihkihkl
sl*ifqlsfqqlqsllqlk*i*lvfknyqiyqd*ngkllmvktmiinhhhqlkkilqrfc
fyffsnanqnfynyy

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE264 (VHE264Q) /CSM/VH/VHE2-C/VHE264Q.Seq.d/                       2676   0.0
VHI312 (VHI312Q) /CSM/VH/VHI3-A/VHI312Q.Seq.d/                       1497   0.0
AHF510 (AHF510Q) /CSM/AH/AHF5-A/AHF510Q.Seq.d/                       1497   0.0
AHE154 (AHE154Q) /CSM/AH/AHE1-C/AHE154Q.Seq.d/                       1495   0.0
AHC524 (AHC524Q) /CSM/AH/AHC5-A/AHC524Q.Seq.d/                       1495   0.0
AHD662 (AHD662Q) /CSM/AH/AHD6-C/AHD662Q.Seq.d/                       1487   0.0
VHQ167 (VHQ167Q) /CSM/VH/VHQ1-C/VHQ167Q.Seq.d/                       1481   0.0
VHK686 (VHK686Q) /CSM/VH/VHK6-D/VHK686Q.Seq.d/                       1481   0.0
VHE461 (VHE461Q) /CSM/VH/VHE4-C/VHE461Q.Seq.d/                       1481   0.0
VHC764 (VHC764Q) /CSM/VH/VHC7-C/VHC764Q.Seq.d/                       1481   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1360   0.0    3
BQ999895|BQ999895.1 QGG23F15.yg.ab1 QG_EFGHJ lettuce serriola Lactuca sativa cDNA clone QGG23F15, mRNA sequence.    44   3e-04  2
BQ793980|BQ793980.1 EST 2918 Veraison Grape berries Lambda Zap II Library Vitis vinifera cDNA clone TT251C04 3', mRNA sequence.    40   0.047  2
EL473838|EL473838.1 CHUL1765.b1_I10.ab1 CHU(LMS) puzzle sunflower Helianthus paradoxus cDNA clone CHUL1765, mRNA sequence.    44   0.049  2
BQ793878|BQ793878.1 EST 2816 Veraison Grape berries Lambda Zap II Library Vitis vinifera cDNA clone TT251B04 3', mRNA sequence.    40   0.050  2
AM569193|AM569193.1 Paracentrotus lividus EST, clone MPMGp1173O11114Q, 5' end.    50   0.17   1
DY809519|DY809519.1 CTOX18042.b1_D24.ab1 CTO(XYZ) dandelion Taraxacum officinale cDNA clone CTOX18042, mRNA sequence.    38   0.22   2
AL672129|AL672129.4 Zebrafish DNA sequence from clone BUSM1-104F18 in linkage group 9  Contains a novel gene for a protein similar to KIAA1548, a novel  gene and a CpG island.    46   0.34   3
AA382162|AA382162.1 EST95325 Activated T-cells II Homo sapiens cDNA 5' end, mRNA sequence.    40   0.35   3
CE529546|CE529546.1 tigr-gss-dog-17000365859317 Dog Library Canis familiaris genomic, genomic survey sequence.    48   0.69   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   421   e-116
(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   421   e-116
BC091843_1(BC091843|pid:none) Danio rerio zgc:110815, mRNA (cDNA...   178   5e-43
AE014134_1973(AE014134|pid:none) Drosophila melanogaster chromos...   168   5e-40
AC148217_12(AC148217|pid:none) Medicago truncatula clone mth2-20...   167   1e-39
BC121882_1(BC121882|pid:none) Xenopus tropicalis lysosomal alpha...   166   1e-39
AY119107_1(AY119107|pid:none) Drosophila melanogaster RE08556 fu...   166   2e-39
AE014134_1633(AE014134|pid:none) Drosophila melanogaster chromos...   166   2e-39
AB026648_12(AB026648|pid:none) Arabidopsis thaliana genomic DNA,...   156   1e-36
AB011474_23(AB011474|pid:none) Arabidopsis thaliana genomic DNA,...   152   3e-35

 psg: 0.80  gvh: 0.35  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for VHE264 is nuc

>VHE264F.Seq
AAAAGTACACCAGAGAATCAAGTTTTCACATCAGTACTACGTGCAATGTATTGTACTCCA
GATGGTTTCAACTTTGAGCAAGGTGATGACCCAATTCAAGATGACCCAAATTTATTCGAT
AATAACGTAGACTCAAGAGCTGAACAGTTCACTCAAGTGGCACTTGAATATGCAACTCAT
TATCGTACCGATAATGTACTAATTCCATTTGGTTGTGATTTCGCTTACCTTAACGCTCAA
ATGTATTACAAAAACATTGACAAACTAATCGCACATATCAATTCAAATCCGGATAAATAT
GGTTTAAATTTGTTATATTCAACTCCATCAATTTATATAGATGCTGTAAATGATGCCAAT
CTAGTATGGGAAGTGAAAACTGATGATCTCTTCCCATATGCTGATAATGAATTTAGTTAT
TGGACTGGTTATTTCGTTAGTCGTCCAGCATTGAAAGGCTACGTTCGTCAAAATAATGCT
CTATTACATGTAGTTGAACAAATGTTGGTAACTAGTAGTAATCTTATGCCAAGTAGTAGA
TCTGAACAATTGGTTGATGACATTGTTATAATGAGAGAGGTAATGGGTATTGCTCAACAT
CATGATGCCGTATCANNNNNNNNNN

>VHE264Z.Seq
NNNNNNNNNNTCCATCATGGAATTTANCCGTGGTTCAACCAACCAGTGGTAATTATGTAC
CTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAATCATTACAATTCACCATTGTCA
CTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGCATC
GTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAA
TCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATC
GTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAACAAT
CTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCAC
CTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATACCA
TACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAA
CTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAA
TGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAAAATGGAAAACTGTTG
ATGGTAAAAACTATGATCATAAATCATCATCATCAACTAAAGAAGATTCTTCAAAGGTTT
TGTTTTTACTTTTTCTCCAATGCAAATCAGAACTTTTATAATTACTAC

>VHE264P.Seq
AAAAGTACACCAGAGAATCAAGTTTTCACATCAGTACTACGTGCAATGTATTGTACTCCA
GATGGTTTCAACTTTGAGCAAGGTGATGACCCAATTCAAGATGACCCAAATTTATTCGAT
AATAACGTAGACTCAAGAGCTGAACAGTTCACTCAAGTGGCACTTGAATATGCAACTCAT
TATCGTACCGATAATGTACTAATTCCATTTGGTTGTGATTTCGCTTACCTTAACGCTCAA
ATGTATTACAAAAACATTGACAAACTAATCGCACATATCAATTCAAATCCGGATAAATAT
GGTTTAAATTTGTTATATTCAACTCCATCAATTTATATAGATGCTGTAAATGATGCCAAT
CTAGTATGGGAAGTGAAAACTGATGATCTCTTCCCATATGCTGATAATGAATTTAGTTAT
TGGACTGGTTATTTCGTTAGTCGTCCAGCATTGAAAGGCTACGTTCGTCAAAATAATGCT
CTATTACATGTAGTTGAACAAATGTTGGTAACTAGTAGTAATCTTATGCCAAGTAGTAGA
TCTGAACAATTGGTTGATGACATTGTTATAATGAGAGAGGTAATGGGTATTGCTCAACAT
CATGATGCCGTATCA----------TCCATCATGGAATTTANCCGTGGTTCAACCAACCA
GTGGTAATTATGTACCTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAATCATTAC
AATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGG
ATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGA
ATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTATG
CTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATGTTTA
CAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCACCAT
TGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATA
ATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGATT
CACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAATCA
CTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAA
AATGGAAAACTGTTGATGGTAAAAACTATGATCATAAATCATCATCATCAACTAAAGAAG
ATTCTTCAAAGGTTTTGTTTTTACTTTTTCTCCAATGCAAATCAGAACTTTTATAATTAC
TAC