>VHE254 (VHE254Q) /CSM/VH/VHE2-C/VHE254Q.Seq.d/
CATAATATAATCCAATATTTCTTTTTTTTTGAAATACAGAAAGTTTGTCACACAAAAATG
ACATTAAACCAAATAATTTTATCATTGATTTTGGGTTTGAGTTTAATGACTACAATCATT
AAATCAGTTCCAATGGACCCAACAATTTATATTGAAGGTACAATTTTTGATCAATGGCCA
GAATATAATTATAATTTTGAAAAAACCAACCCAGGAAGTAATGTTAAAACCCTTGGAATG
GTAAAAGCAAAATTGAACTCAACAACAAAAGTTCCAGAACTTTCTTCAGATGATACTAAA
ACTTCACCAAATACATTAGGTACTATGCAATATCCAGAGTTATTTAAATATCACTTTACA
TCAAATGTTGATGCACCAGCATCCAAAAACTCTGGTAAAAATTTACCAATGAAAATACAA
TTCAAATTAGATCAAGACAATAGTGGTTTTTATACTTTTTTCAATCAAACTTTTTTCCCA
ATTGATAACATGGGCTACGATGTAGATCCATCATTTAGAATTTATAAACAAAGTGGTAAC
TATCATAATTTCCATTTTTGTGTAAAGATGAATAGTCGTTTTACTTATAATGGAGGGTGA
AGTTTTTAATTTTAAAGGTGATGATGATTTATGGGTATATATTGATGATAAXXXXXXXXX
XCTCCAGTTAAAGTTGGTGCAGGTGTTGGTGAATGTGTAACTACCAATACAACATGTGCA
CCAACCACAAACGTTGATAAATGTAAAAACTACCTTTGCGATTCTGAAAAAGGTTGTTAT
GAAACCGATGTAGTTTGTCCAGATAGTGGAAATTGTAAAGTTGGTCATTGTGATTCCAAG
GTAGGTTGCTCTTTGAAGCCACGTGTCTGTAAATCACCAGCTTTTTGCATCGTAGCAGAA
TGTGATGAAACTGTTGGTTGCATTGCATTTGAGAGACGTTGTGCATCAAGTAATCCAAAA
TGTAAAGCAGGTATTTGTGTAAATGGTACAACACCAGAAGAAGGTGAATGTGAATCTGTA
AATTATGATCCAAAACCATTTATTTGTCAAACTGGTGCTATAGTTTCTACTGCTGTAATT
GCTGGTGTAACTGTTGCTGGTGCTGTTGCTCTTGGTATTTTCATTTATGGTGGTAAACGT
GGTTATGATTATTGGAAAGAAAGTAGAAGCATTTCTATGAGTTCTGCTGTTTCAAGTCCA
ATGTATGTTCCTTCTGCAAATGCTTCNAAAATCCACTT

ii*snisfflkyrkfvtqk*h*tk*fyh*fwv*v**lqslnqfqwtqqfilkvqflingq
niiiilkkptqevmlkplew*kqn*tqqqkfqnflqmilklhqih*vlcniqsylnitlh
qmlmhqhpktlvkiyq*kynsn*iktivvfilfsiklfsqlitwatm*ihhlefinkvvt
iiisifv*r*IVVLLIMEGEVFNFKGDDDLWVYIDD---

---PVKVGAGVGECVTTNTTCAPTTNVDKCKNYLCDSEKGCYETDVVCPDSGNCKVGHCD
SKVGCSLKPRVCKSPAFCIVAECDETVGCIAFERRCASSNPKCKAGICVNGTTPEEGECE
SVNYDPKPFICQTGAIVSTAVIAGVTVAGAVALGIFIYGGKRGYDYWKESRSISMSSAVS
SPMYVPSANASKIH

Frame A:
hniiqyffffeiqkvchtkmtlnqiilslilglslmttiiksvpmdptiyiegtifdqwp
eynynfektnpgsnvktlgmvkaklnsttkvpelssddtktspntlgtmqypelfkyhft
snvdapasknsgknlpmkiqfkldqdnsgfytffnqtffpidnmgydvdpsfriykqsgn
yhnfhfcvkmnsrftyngg*sf*f*r***fmgiy***---

---lqlklvqvlvnv*lpiqhvhqpqtlinvkttfailkkvvmkpm*fvqiveivklviv
ipr*val*shvsvnhqlfas*qnvmkllvalhlrdvvhqviqnvkqvfv*mvqhqkkvnv
nl*imiqnhlfvklvl*flll*llv*lllvllllvfsfmvvnvvmiigkkveafl*vllf
qvqcmfllqmlxkst

Frame B:
ii*snisfflkyrkfvtqk*h*tk*fyh*fwv*v**lqslnqfqwtqqfilkvqflingq
niiiilkkptqevmlkplew*kqn*tqqqkfqnflqmilklhqih*vlcniqsylnitlh
qmlmhqhpktlvkiyq*kynsn*iktivvfilfsiklfsqlitwatm*ihhlefinkvvt
iiisifv*r*IVVLLIMEGEVFNFKGDDDLWVYIDD---

---ss*swcrcw*mcnyqynmctnhkr**m*klplrf*krll*nrcslsr*wkl*swsl*
fqgrllfeatcl*itsflhrsrm**ncwlhci*etlcik*skm*srylckwyntrrr*m*
ickl*sktiylsnwcysfyccncwcnccwcccswyfhlww*twl*llerk*khfyefccf
ksnvcsfckcfxnpl

Frame C:
*ynpiflff*nteslshkndikpnnfiidfgfefndynh*issngpnnly*rynf*smar
i*l*f*knqprk*c*npwngkskielnnkssrtffr*y*nftkyiryyaisrvi*islyi
kc*ctsiqklw*kftnentiqirsrq*wflyffqsnffpn**hglrcrsii*nl*tkw*l
s*fpflckde*sfyl*wrvkflilkvmmiygyilmi---

---PVKVGAGVGECVTTNTTCAPTTNVDKCKNYLCDSEKGCYETDVVCPDSGNCKVGHCD
SKVGCSLKPRVCKSPAFCIVAECDETVGCIAFERRCASSNPKCKAGICVNGTTPEEGECE
SVNYDPKPFICQTGAIVSTAVIAGVTVAGAVALGIFIYGGKRGYDYWKESRSISMSSAVS
SPMYVPSANASKIH

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE254 (VHE254Q) /CSM/VH/VHE2-C/VHE254Q.Seq.d/                       1445   0.0
VHE263 (VHE263Q) /CSM/VH/VHE2-C/VHE263Q.Seq.d/                       1138   0.0
VHO887 (VHO887Q) /CSM/VH/VHO8-D/VHO887Q.Seq.d/                       1124   0.0
VHO838 (VHO838Q) /CSM/VH/VHO8-B/VHO838Q.Seq.d/                       1124   0.0
VHN167 (VHN167Q) /CSM/VH/VHN1-C/VHN167Q.Seq.d/                       1124   0.0
VHN157 (VHN157Q) /CSM/VH/VHN1-C/VHN157Q.Seq.d/                       1124   0.0
VHD260 (VHD260Q) /CSM/VH/VHD2-C/VHD260Q.Seq.d/                       1124   0.0
VHB861 (VHB861Q) /CSM/VH/VHB8-C/VHB861Q.Seq.d/                       1124   0.0
VHA729 (VHA729Q) /CSM/VH/VHA7-B/VHA729Q.Seq.d/                       1124   0.0
VFC520 (VFC520Q) /CSM/VF/VFC5-A/VFC520Q.Seq.d/                       1124   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   920   0.0    8
U67940|U67940.1 Dictyostelium discoideum ORFveg106 mRNA, complete cds.   161   1e-44  5
AB053203|AB053203.1 Dictyostelium discoideum psiA mRNA for Psi facor, complete cds.    38   6e-04  4
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.    46   0.080  4
AC137804|AC137804.1 Homo sapiens chromosome 16 clone RP11-78G5, WORKING DRAFT SEQUENCE, 1 unordered piece.    50   0.16   1
AC137784|AC137784.1 Homo sapiens chromosome 16 clone RP11-1407F4, WORKING DRAFT SEQUENCE, 49 unordered pieces.    50   0.16   1
AC106014|AC106014.1 Homo sapiens chromosome 8 clone CTD-2064I9 map 8, LOW-PASS SEQUENCE SAMPLING.    50   0.16   1
AC021420|AC021420.3 Homo sapiens chromosome 4 clone RP11-92K5 map 4, WORKING DRAFT SEQUENCE, 17 unordered pieces.    50   0.16   1
AC093783|AC093783.2 Homo sapiens BAC clone RP11-209D20 from 4, complete sequence.    50   0.16   1
AM285321|AM285321.1 Spiroplasma citri GII3-3X chromosome, contig Contig20.    42   0.24   6

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86HT1) RecName: Full=Protein psiL; Flags: Precursor;  &AC11695...   343   6e-93
(Q94494) RecName: Full=Protein psiH; Flags: Precursor;  &U67940_...   165   3e-39
(Q54VS2) RecName: Full=Protein psiP; Flags: Precursor;                155   3e-36
(Q54P69) RecName: Full=Protein psiM; Flags: Precursor;                149   2e-34
(Q557E9) RecName: Full=Protein psiG; Flags: Precursor;  &AC11568...   137   6e-31
(Q55FQ4) RecName: Full=Protein psiB; Flags: Precursor;                123   1e-26
(Q968Z6) RecName: Full=Protein psiA; AltName: Full=Prespore-cell...   114   9e-24
(Q55FQ5) RecName: Full=Protein psiS; Flags: Precursor;                109   2e-22
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                100   2e-19
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                 93   2e-17

 psg: 0.75  gvh: 0.42  alm: 0.21  top: 0.93  tms: 0.07  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VHE254 is cyt

>VHE254F.Seq
CATAATATAATCCAATATTTCTTTTTTTTTGAAATACAGAAAGTTTGTCACACAAAAATG
ACATTAAACCAAATAATTTTATCATTGATTTTGGGTTTGAGTTTAATGACTACAATCATT
AAATCAGTTCCAATGGACCCAACAATTTATATTGAAGGTACAATTTTTGATCAATGGCCA
GAATATAATTATAATTTTGAAAAAACCAACCCAGGAAGTAATGTTAAAACCCTTGGAATG
GTAAAAGCAAAATTGAACTCAACAACAAAAGTTCCAGAACTTTCTTCAGATGATACTAAA
ACTTCACCAAATACATTAGGTACTATGCAATATCCAGAGTTATTTAAATATCACTTTACA
TCAAATGTTGATGCACCAGCATCCAAAAACTCTGGTAAAAATTTACCAATGAAAATACAA
TTCAAATTAGATCAAGACAATAGTGGTTTTTATACTTTTTTCAATCAAACTTTTTTCCCA
ATTGATAACATGGGCTACGATGTAGATCCATCATTTAGAATTTATAAACAAAGTGGTAAC
TATCATAATTTCCATTTTTGTGTAAAGATGAATAGTCGTTTTACTTATAATGGAGGGTGA
AGTTTTTAATTTTAAAGGTGATGATGATTTATGGGTATATATTGATGATAANNNNNNNNN
N

>VHE254Z.Seq
NNNNNNNNNNCTCCAGTTAAAGTTGGTGCAGGTGTTGGTGAATGTGTAACTACCAATACA
ACATGTGCACCAACCACAAACGTTGATAAATGTAAAAACTACCTTTGCGATTCTGAAAAA
GGTTGTTATGAAACCGATGTAGTTTGTCCAGATAGTGGAAATTGTAAAGTTGGTCATTGT
GATTCCAAGGTAGGTTGCTCTTTGAAGCCACGTGTCTGTAAATCACCAGCTTTTTGCATC
GTAGCAGAATGTGATGAAACTGTTGGTTGCATTGCATTTGAGAGACGTTGTGCATCAAGT
AATCCAAAATGTAAAGCAGGTATTTGTGTAAATGGTACAACACCAGAAGAAGGTGAATGT
GAATCTGTAAATTATGATCCAAAACCATTTATTTGTCAAACTGGTGCTATAGTTTCTACT
GCTGTAATTGCTGGTGTAACTGTTGCTGGTGCTGTTGCTCTTGGTATTTTCATTTATGGT
GGTAAACGTGGTTATGATTATTGGAAAGAAAGTAGAAGCATTTCTATGAGTTCTGCTGTT
TCAAGTCCAATGTATGTTCCTTCTGCAAATGCTTCNAAAATCCACTT

>VHE254P.Seq
CATAATATAATCCAATATTTCTTTTTTTTTGAAATACAGAAAGTTTGTCACACAAAAATG
ACATTAAACCAAATAATTTTATCATTGATTTTGGGTTTGAGTTTAATGACTACAATCATT
AAATCAGTTCCAATGGACCCAACAATTTATATTGAAGGTACAATTTTTGATCAATGGCCA
GAATATAATTATAATTTTGAAAAAACCAACCCAGGAAGTAATGTTAAAACCCTTGGAATG
GTAAAAGCAAAATTGAACTCAACAACAAAAGTTCCAGAACTTTCTTCAGATGATACTAAA
ACTTCACCAAATACATTAGGTACTATGCAATATCCAGAGTTATTTAAATATCACTTTACA
TCAAATGTTGATGCACCAGCATCCAAAAACTCTGGTAAAAATTTACCAATGAAAATACAA
TTCAAATTAGATCAAGACAATAGTGGTTTTTATACTTTTTTCAATCAAACTTTTTTCCCA
ATTGATAACATGGGCTACGATGTAGATCCATCATTTAGAATTTATAAACAAAGTGGTAAC
TATCATAATTTCCATTTTTGTGTAAAGATGAATAGTCGTTTTACTTATAATGGAGGGTGA
AGTTTTTAATTTTAAAGGTGATGATGATTTATGGGTATATATTGATGATAA---------
-CTCCAGTTAAAGTTGGTGCAGGTGTTGGTGAATGTGTAACTACCAATACAACATGTGCA
CCAACCACAAACGTTGATAAATGTAAAAACTACCTTTGCGATTCTGAAAAAGGTTGTTAT
GAAACCGATGTAGTTTGTCCAGATAGTGGAAATTGTAAAGTTGGTCATTGTGATTCCAAG
GTAGGTTGCTCTTTGAAGCCACGTGTCTGTAAATCACCAGCTTTTTGCATCGTAGCAGAA
TGTGATGAAACTGTTGGTTGCATTGCATTTGAGAGACGTTGTGCATCAAGTAATCCAAAA
TGTAAAGCAGGTATTTGTGTAAATGGTACAACACCAGAAGAAGGTGAATGTGAATCTGTA
AATTATGATCCAAAACCATTTATTTGTCAAACTGGTGCTATAGTTTCTACTGCTGTAATT
GCTGGTGTAACTGTTGCTGGTGCTGTTGCTCTTGGTATTTTCATTTATGGTGGTAAACGT
GGTTATGATTATTGGAAAGAAAGTAGAAGCATTTCTATGAGTTCTGCTGTTTCAAGTCCA
ATGTATGTTCCTTCTGCAAATGCTTCNAAAATCCACTT