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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFB623
Library AF
(Link to library)
Clone ID AFB623
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11996-1
Original site URL
Representative seq. ID AFB623P
(Link to Original site)
Representative DNA sequence 
>AFB623 (AFB623Q) /CSM/AF/AFB6-A/AFB623Q.Seq.d/
AAGATATAAAATTACAGACATTTAGCAATAAATATAAAAGTACCAGTACATTTACTTACA
ACCCTATTTAATAGTAGGGATAGTTAATAATATAAATATAAATAACAACAATAAGAACAA
TAAAAAAAAAAXXXXXXXXXXTGATTCACATCTTACTCTTGTTATGGAATTGGTAACTGG
TGGTGAATTATTTTATAAAATTGTTGAAAGAGGTTCATTTACAGAAAAAGGTGCACGTAA
TGTTGTAAGACAAGTATGTGCTGGTGTTGAATATCTCCATTCACAAGGTATTGCTCATAG
AGATTTGAAACCAGAGAATTTATTATGTAATGGTGAAGGTGAAGATATGACAATCAAAAT
TGCAGATTTTGGTCTTAGTAAAATATTTGGAACTGGTGAAGCATTGGAAACAAGTTGTGG
TACACCAGATTATGTTGCACCAGAAGTTTTAACTGGTGGTTCATATGATAATGCAGTCGA
TATGTGGTCAATTGGTGTCATTACCTATATTTTATTATGTGGTTTCCCACCATTCTATGC
TTCATCTCAAAACTTATTATTTGAAAAAATTTCAACCGCTGATTATGATTTCCCAGAACC
AGAATGGACTCATGTCTCTGAACATGCCAAAGCTTNCATTAGAAACTTAANTGTCAAAGA
NCCAGATCAACGTCATACNGCCAAACAATGTCTTGATGACCTTTGGCTCTCTGGTAGTGA
TCAATCAANT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
rykitdi*q*i*KYQYIYLQPYLIVGIVNNININNNNKNNKKK---

---DSHLTLVMELVTGGELFYKIVERGSFTEKGARNVVRQVCAGVEYLHSQGIAHRDLKP
ENLLCNGEGEDMTIKIADFGLSKIFGTGEALETSCGTPDYVAPEVLTGGSYDNAVDMWSI
GVITYILLCGFPPFYASSQNLLFEKISTADYDFPEPEWTHVSEHAKAXIRNLXVKXPDQR
HTAKQCLDDLWLSGSDQSX
Translated Amino Acid sequence (All Frames) 
Frame A:
ki*nyrhlainikvpvhllttlfnsrds**ykyk*qq*eq*kk---

---*ftsyscygignww*iil*nc*krfiyrkrct*ccktsmcwc*ispftrycs*rfet
refim*w*r*rydnqncrfws**niwnw*signklwytrlcctrsfnwwfi**csryvvn
wchylyfimwfptilcfisklii*knfnr*l*fprtrmdscl*tcqsxh*klxcqrxrst
syxqtms**plalw**six

Frame B:
rykitdi*q*i*KYQYIYLQPYLIVGIVNNININNNNKNNKKK---

---DSHLTLVMELVTGGELFYKIVERGSFTEKGARNVVRQVCAGVEYLHSQGIAHRDLKP
ENLLCNGEGEDMTIKIADFGLSKIFGTGEALETSCGTPDYVAPEVLTGGSYDNAVDMWSI
GVITYILLCGFPPFYASSQNLLFEKISTADYDFPEPEWTHVSEHAKAXIRNLXVKXPDQR
HTAKQCLDDLWLSGSDQSX

Frame C:
diklqtfsnkykststftynpi***g*lii*i*ittirtikkk---

---ihillllwnw*lvvnyfikllkevhlqkkvhvml*dkyvlvlnisihkvlliei*nq
riyyvmvkvki*qsklqilvlvkylelvkhwkqvvvhqimlhqkf*lvvhmimqsicgql
vslpifyyvvshhsmlhlktyylkkfqplimisqnqnglmslnmpklxlet*xskxqinv
ixpnnvlmtfgslvvinq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFB623 (AFB623Q) /CSM/AF/AFB6-A/AFB623Q.Seq.d/                       1140   0.0
AFL780 (AFL780Q) /CSM/AF/AFL7-D/AFL780Q.Seq.d/                       1132   0.0
SFK820 (SFK820Q) /CSM/SF/SFK8-A/SFK820Q.Seq.d/                       1065   0.0
AFL880 (AFL880Q) /CSM/AF/AFL8-D/AFL880Q.Seq.d/                       1059   0.0
SSI101 (SSI101Q) /CSM/SS/SSI1-A/SSI101Q.Seq.d/                       1023   0.0
SFK552 (SFK552Q) /CSM/SF/SFK5-C/SFK552Q.Seq.d/                        985   0.0
SFH150 (SFH150Q) /CSM/SF/SFH1-C/SFH150Q.Seq.d/                        971   0.0
SSC643 (SSC643Q) /CSM/SS/SSC6-B/SSC643Q.Seq.d/                        965   0.0
VFJ379 (VFJ379Q) /CSM/VF/VFJ3-D/VFJ379Q.Seq.d/                        957   0.0
CFB218 (CFB218Q) /CSM/CF/CFB2-A/CFB218Q.Seq.d/                        952   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.  1072  0.0  3
M64176|M64176.1 D.discoideum myosin light chain kinase (MLCK) mRNA, complete cds.    84  2e-39  4
AZ667950|AZ667950.1 ENTFN88TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52  8e-15  3
AZ545332|AZ545332.1 ENTGA35TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52  8e-11  3
BG809925|BG809925.1 mgct001xp07f Magnaporthe grisea Appressorium Stage cDNA Magnaporthe grisea cDNA clone mgct001xp07f 5', mRNA sequence.    50  5e-09  2
BI510179|BI510179.1 BB170031A10H07 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170031A10H07 5', mRNA sequence.    68  2e-07  1
AZ930835|AZ930835.1 474.dhz59b07.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomyces unisporus genomic clone 474.dhz59b07.s1, DNA sequence.    48  2e-07  3
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    50  2e-07  9
BH137166|BH137166.1 ENTNR83TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52  1e-06  2
AZ672839|AZ672839.1 ENTIX25TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52  1e-06  2
dna update 2003. 7.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q869W6) RecName: Full=Probable calcium/calmodulin-dependent ser...   394   e-108
AC116982_54(AC116982|pid:none) Dictyostelium discoideum chromoso...   238   1e-61
AB076903_1(AB076903|pid:none) Ciona intestinalis Ci-CaM-K mRNA f...   222   1e-56
BC074183_1(BC074183|pid:none) Xenopus laevis MGC82022 protein, m...   221   2e-56
AK052401_1(AK052401|pid:none) Mus musculus 13 days embryo lung c...   218   1e-55
(Q8IU85) RecName: Full=Calcium/calmodulin-dependent protein kina...   218   1e-55
AY335686_1(AY335686|pid:none) Synthetic construct Homo sapiens C...   218   1e-55
(Q8BW96) RecName: Full=Calcium/calmodulin-dependent protein kina...   218   1e-55
BC110966_1(BC110966|pid:none) Xenopus laevis calcium/calmodulin-...   217   3e-55
BC041721_1(BC041721|pid:none) Xenopus laevis calcium/calmodulin-...   215   1e-54
protein update 2009. 3.27
PSORT 

 psg: 0.90  gvh: 0.49  alm: 0.34  top: 0.57  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFB623 is cyt
5' end seq. ID AFB623F
5' end seq. 
>AFB623F.Seq
AAGATATAAAATTACAGACATTTAGCAATAAATATAAAAGTACCAGTACATTTACTTACA
ACCCTATTTAATAGTAGGGATAGTTAATAATATAAATATAAATAACAACAATAAGAACAA
TAAAAAAAAAA----------
Length of 5' end seq. 131
3' end seq. ID AFB623Z
3' end seq. 
>AFB623Z.Seq
----------TGATTCACATCTTACTCTTGTTATGGAATTGGTAACTGGTGGTGAATTAT
TTTATAAAATTGTTGAAAGAGGTTCATTTACAGAAAAAGGTGCACGTAATGTTGTAAGAC
AAGTATGTGCTGGTGTTGAATATCTCCATTCACAAGGTATTGCTCATAGAGATTTGAAAC
CAGAGAATTTATTATGTAATGGTGAAGGTGAAGATATGACAATCAAAATTGCAGATTTTG
GTCTTAGTAAAATATTTGGAACTGGTGAAGCATTGGAAACAAGTTGTGGTACACCAGATT
ATGTTGCACCAGAAGTTTTAACTGGTGGTTCATATGATAATGCAGTCGATATGTGGTCAA
TTGGTGTCATTACCTATATTTTATTATGTGGTTTCCCACCATTCTATGCTTCATCTCAAA
ACTTATTATTTGAAAAAATTTCAACCGCTGATTATGATTTCCCAGAACCAGAATGGACTC
ATGTCTCTGAACATGCCAAAGCTTNCATTAGAAACTTAANTGTCAAAGANCCAGATCAAC
GTCATACNGCCAAACAATGTCTTGATGACCTTTGGCTCTCTGGTAGTGATCAATCAANT
Length of 3' end seq. 589
Connected seq. ID AFB623P
Connected seq. 
>AFB623P.Seq
AAGATATAAAATTACAGACATTTAGCAATAAATATAAAAGTACCAGTACATTTACTTACA
ACCCTATTTAATAGTAGGGATAGTTAATAATATAAATATAAATAACAACAATAAGAACAA
TAAAAAAAAAA----------TGATTCACATCTTACTCTTGTTATGGAATTGGTAACTGG
TGGTGAATTATTTTATAAAATTGTTGAAAGAGGTTCATTTACAGAAAAAGGTGCACGTAA
TGTTGTAAGACAAGTATGTGCTGGTGTTGAATATCTCCATTCACAAGGTATTGCTCATAG
AGATTTGAAACCAGAGAATTTATTATGTAATGGTGAAGGTGAAGATATGACAATCAAAAT
TGCAGATTTTGGTCTTAGTAAAATATTTGGAACTGGTGAAGCATTGGAAACAAGTTGTGG
TACACCAGATTATGTTGCACCAGAAGTTTTAACTGGTGGTTCATATGATAATGCAGTCGA
TATGTGGTCAATTGGTGTCATTACCTATATTTTATTATGTGGTTTCCCACCATTCTATGC
TTCATCTCAAAACTTATTATTTGAAAAAATTTCAACCGCTGATTATGATTTCCCAGAACC
AGAATGGACTCATGTCTCTGAACATGCCAAAGCTTNCATTAGAAACTTAANTGTCAAAGA
NCCAGATCAACGTCATACNGCCAAACAATGTCTTGATGACCTTTGGCTCTCTGGTAGTGA
TCAATCAANT
Length of connected seq. 720
Full length Seq ID -
Full length Seq. -
Length of full length seq. -