>VHE244 (VHE244Q) /CSM/VH/VHE2-B/VHE244Q.Seq.d/
GTTGGCAAAGTGCTGATTTACTTTGTGGAGCATGGTTAAAGGCCAGATTTAACTATCTGT
CAGCTAACAATGTAACATTTGATGAATCTTTTTAATTGGTAATAAAAGTGACGGGATCTA
ATCCTGTAATTTTGTGACCTCTTCAAAGGGAAACATTAACCAAACAATCAAAACAAAAAA
TAAGTTTTGTAATTAATATGTAATAGTGATTAATATCATGATACGTGTTATCTACATGCT
TAACTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCT
AAGTATAAATTCTTGTACGATGAAACTGCAGACGGCTCATTACAACAGTGATAAACTAAT
AGACTTTCGGGTTTTACCTTTTGGATAACCGCAGTAAATCGGGGCTAATACATACAAGCG
ATGGGTGACTGGCAACGGAAGCTCAGCGATTATTAGCATTCTACCAATGCCTTCGGGTTT
TGGGTGATACCGAATAATATTGCAGATCGAGGATTTATCTTCGACAAGTCTACTGTGTCA
CTGCCCTATCAACTTTCGATGGTACGGTATTGGCCTACCATGGTTGTAACGGGTAXXXXX
XXXXXCTTAGTTGGTGGAGCGATTTGTCTGGTCAATTCCGATAACGGACGAGACCTCGAC
CTGCTAACTAGTAGTATTTATTAGTCGATATAGACGATAGCTTTTCTGGGGTTTGGAATG
ATTTCGGTCATCTCCTGCTTCAAGGAGTGTGTAGTCTGACTCGATAGGTACGAATTAAAA
CTTCTTAGAGGGACTACCTGCCTCAAGCAGGCGGAAGTCCGAGGCAATAACAGGTCTGTG
ATGCCCTTAGATACCTTGGGCCGCACGCGCGCTACAATGTAGGAAACAAAAAGGCTCCTG
GTCCGGAAGGATTGGGTAATCATTTGAATTTCCTACGTAACTGGGCTTGATCTTTGTAAT
TATTGATCATAAACGAGGAATTCCTTGTAAGCGTAAGTCATTACCTTATGCTGAATATGT
CCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATCGAATGATACGGTAAAGTTAA
CGGATCGTTTTATCTGTGGCAACACTGATATAAATTAAAAGTTATTTAAATCTCATTGTT
TAGAGGAAGGAGAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGATGGATCATTTTT
TATCTTAATATCATAAACTTTATGTTTTTTTGGATAATGGAACTGCTTGCGTATAAAGAA
ATTAGGCTTTTTAATTAAGGTTTAAGNAGTAAGTGGNCTGTCAGATTTA

vgkvliyfvehg*rpdlticqltm*hlmnlfnw**k*rdlil*fcdlfkgkh*pnnqnkk
*vl*licnsd*yhdtcylha*lvdpasshmlvskikpcmskykflydetadgslqq**tn
rlsgftfwitavnrg*yiqamgdwqrklsdy*HSTNAFGFWVIPNNIADRGFIFDKSTVS
LPYQLSMVRYWPTMVVTG---

---LVGGAICLVNSDNGRDLDLLTSSIY*si*tiaflgfgmisviscfkecvv*ldryel
kllrgttclkqaevrgnnrsvmpldtlgrtratm*etkrllvrkdwvii*isyvtgldlc
ny*s*trnsl*a*vitlc*icpcplytppvaptdrmir*s*rivlsvatli*iksylnli
v*rkeks*qgirr*tcgwiifylniinfmffwimellaykeirlfn*glxskwxvrf

Frame A:
vgkvliyfvehg*rpdlticqltm*hlmnlfnw**k*rdlil*fcdlfkgkh*pnnqnkk
*vl*licnsd*yhdtcylha*lvdpasshmlvskikpcmskykflydetadgslqq**tn
rlsgftfwitavnrg*yiqamgdwqrklsdy*HSTNAFGFWVIPNNIADRGFIFDKSTVS
LPYQLSMVRYWPTMVVTG---

---lswwsdlsgqfr*rtrprpan**yllvdiddsfsgvwndfghlllqgvcsltr*vri
kts*rdylpqaggsprq*qvcdalrylgpharynvgnkkapgpeglgnhlnflrnwa*sl
*lliineeflvsvshylmlnmslpfvhtarrsyrsndtvkltdrficgntdin*klfksh
cleegevvtryp*vnlrmdhfls*yhklyvfldngtacv*rn*af*lrfkx*vxcqi

Frame B:
lakc*ftlwsmvkgqi*lsvs*qcni**iflignksdgi*scnfvtsskgninqtiktkn
kfcn*yvivinimirviymlnwlilpvviclsqrlshaclsinsctmklqtahynsdkli
dfrvlpfg*pq*igantykrwvtgngssaiisilpmpsgfg*yriilqiedlsstsllch
cpinfrwygiglpwl*rv---

---LVGGAICLVNSDNGRDLDLLTSSIY*si*tiaflgfgmisviscfkecvv*ldryel
kllrgttclkqaevrgnnrsvmpldtlgrtratm*etkrllvrkdwvii*isyvtgldlc
ny*s*trnsl*a*vitlc*icpcplytppvaptdrmir*s*rivlsvatli*iksylnli
v*rkeks*qgirr*tcgwiifylniinfmffwimellaykeirlfn*glxskwxvrf

Frame C:
wqsadllcgawlkarfnylsannvtfdesf*lvikvtgsnpvil*plqretltkqskqki
sfvinm***lis*yvlstcltg*scq*syaclkd*amhv*v*ilvr*ncrrlittvin**
tfgfylldnrsksglihtsdg*lateaqrllafyqclrvlgdte*ycrsriylrqvycvt
alstfdgtvlayhgcng---

---*lverfvwsipitdetstc*lvvfisryrr*lfwgle*frsspasrsv*sdsigtn*
nfleglpassrrkseaitgl*cp*ipwaaralqcrkqkgswsgrig*sfefpt*lglifv
iidhkrgipckrkslpyaeyvpalcthrpsllpie*ygkvngsfylwqh*yklkvi*isl
frgrrsrnkvsvgepadgsffilis*tlcffg*wnclrikklgflikv*xvsglsdl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE244 (VHE244Q) /CSM/VH/VHE2-B/VHE244Q.Seq.d/                       2482   0.0
AHO642 (AHO642Q) /CSM/AH/AHO6-B/AHO642Q.Seq.d/                       1340   0.0
VHK855 (VHK855Q) /CSM/VH/VHK8-C/VHK855Q.Seq.d/                       1326   0.0
VHK334 (VHK334Q) /CSM/VH/VHK3-B/VHK334Q.Seq.d/                       1326   0.0
VFM557 (VFM557Q) /CSM/VF/VFM5-C/VFM557Q.Seq.d/                       1326   0.0
AHL576 (AHL576Q) /CSM/AH/AHL5-D/AHL576Q.Seq.d/                       1322   0.0
VHJ647 (VHJ647Q) /CSM/VH/VHJ6-B/VHJ647Q.Seq.d/                       1314   0.0
VFD795 (VFD795Q) /CSM/VF/VFD7-D/VFD795Q.Seq.d/                       1312   0.0
AHK528 (AHK528Q) /CSM/AH/AHK5-B/AHK528Q.Seq.d/                       1312   0.0
FC-AG24 (FC-AG24Q) /CSM/FC/FC-AG/FC-AG24Q.Seq.d/                     1310   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY171066|AY171066.1 Dictyostelium discoideum extrachromosomal palindromic ribosomal RNA gene sequence, central segment.  1227   0.0    2
X00134|X00134.1 Dictyostelium discoideum 17S rRNA sequence.      1178   0.0    1
K02641|K02641.1 Slime mold (D.discoideum) 17S small subunit ribosomal RNA gene, complete coding sequence.  1178   0.0    1
DQ340385|DQ340385.1 Dictyostelium citrinum 17S ribosomal RNA, 5.8S ribosomal RNA, 26S ribosomal RNA, and 5S ribosomal RNA genes, complete sequence.  1164   0.0    2
AM168071|AM168071.1 Dictyostelium discoideum 18S rRNA gene, isolate NC4.  1154   0.0    1
AM168038|AM168038.1 Dictyostelium dimigraformum 18S rRNA gene, isolate AR5b.  1146   0.0    1
AM168039|AM168039.1 Dictyostelium discoideum 18S rRNA gene, isolate V34.  1130   0.0    1
AM168040|AM168040.1 Dictyostelium discoideum 18S rRNA gene, isolate 91HO-9.  1116   0.0    1
AM168033|AM168033.1 Dictyostelium citrinum 18S rRNA gene, isolate OH494.  1114   0.0    1
X00601|X00601.1 Dictyostelium discoideum ribosomal RNA gene.      979   0.0    2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF421549_1(AF421549|pid:none) Gallus gallus CDH1-D mRNA, partial...    77   1e-12
AM462492_6(AM462492|pid:none) Vitis vinifera contig VV78X177628....    60   1e-07
BA000023_1285(BA000023|pid:none) Sulfolobus tokodaii str. 7 DNA,...    42   8e-06
AY914930_1(AY914930|pid:none) Schistosoma japonicum SJCHGC01014 ...    53   3e-05
EU955514_1(EU955514|pid:none) Zea mays clone 1535262 hypothetica...    50   1e-04
AL844509_687(AL844509|pid:none) Plasmodium falciparum 3D7 chromo...    50   2e-04
AM085146_9(AM085146|pid:none) Magnetospirillum gryphiswaldense m...    42   0.049
CP000890_242(CP000890|pid:none) Coxiella burnetii RSA 331, compl...    39   0.32
CP000930_2283(CP000930|pid:none) Heliobacterium modesticaldum Ic...    39   0.54
AP009386_1894(AP009386|pid:none) Burkholderia multivorans ATCC 1...    37   2.0

 psg: 0.93  gvh: 0.60  alm: 0.33  top: 0.43  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: extracellular, including cell wall
	 16.0 %: mitochondrial
	  4.0 %: vacuolar

>> prediction for VHE244 is cyt

>VHE244F.Seq
GTTGGCAAAGTGCTGATTTACTTTGTGGAGCATGGTTAAAGGCCAGATTTAACTATCTGT
CAGCTAACAATGTAACATTTGATGAATCTTTTTAATTGGTAATAAAAGTGACGGGATCTA
ATCCTGTAATTTTGTGACCTCTTCAAAGGGAAACATTAACCAAACAATCAAAACAAAAAA
TAAGTTTTGTAATTAATATGTAATAGTGATTAATATCATGATACGTGTTATCTACATGCT
TAACTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCT
AAGTATAAATTCTTGTACGATGAAACTGCAGACGGCTCATTACAACAGTGATAAACTAAT
AGACTTTCGGGTTTTACCTTTTGGATAACCGCAGTAAATCGGGGCTAATACATACAAGCG
ATGGGTGACTGGCAACGGAAGCTCAGCGATTATTAGCATTCTACCAATGCCTTCGGGTTT
TGGGTGATACCGAATAATATTGCAGATCGAGGATTTATCTTCGACAAGTCTACTGTGTCA
CTGCCCTATCAACTTTCGATGGTACGGTATTGGCCTACCATGGTTGTAACGGGTANNNNN
NNNNN

>VHE244Z.Seq
NNNNNNNNNNCTTAGTTGGTGGAGCGATTTGTCTGGTCAATTCCGATAACGGACGAGACC
TCGACCTGCTAACTAGTAGTATTTATTAGTCGATATAGACGATAGCTTTTCTGGGGTTTG
GAATGATTTCGGTCATCTCCTGCTTCAAGGAGTGTGTAGTCTGACTCGATAGGTACGAAT
TAAAACTTCTTAGAGGGACTACCTGCCTCAAGCAGGCGGAAGTCCGAGGCAATAACAGGT
CTGTGATGCCCTTAGATACCTTGGGCCGCACGCGCGCTACAATGTAGGAAACAAAAAGGC
TCCTGGTCCGGAAGGATTGGGTAATCATTTGAATTTCCTACGTAACTGGGCTTGATCTTT
GTAATTATTGATCATAAACGAGGAATTCCTTGTAAGCGTAAGTCATTACCTTATGCTGAA
TATGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATCGAATGATACGGTAAA
GTTAACGGATCGTTTTATCTGTGGCAACACTGATATAAATTAAAAGTTATTTAAATCTCA
TTGTTTAGAGGAAGGAGAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGATGGATCA
TTTTTTATCTTAATATCATAAACTTTATGTTTTTTTGGATAATGGAACTGCTTGCGTATA
AAGAAATTAGGCTTTTTAATTAAGGTTTAAGNAGTAAGTGGNCTGTCAGATTTA

>VHE244P.Seq
GTTGGCAAAGTGCTGATTTACTTTGTGGAGCATGGTTAAAGGCCAGATTTAACTATCTGT
CAGCTAACAATGTAACATTTGATGAATCTTTTTAATTGGTAATAAAAGTGACGGGATCTA
ATCCTGTAATTTTGTGACCTCTTCAAAGGGAAACATTAACCAAACAATCAAAACAAAAAA
TAAGTTTTGTAATTAATATGTAATAGTGATTAATATCATGATACGTGTTATCTACATGCT
TAACTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCT
AAGTATAAATTCTTGTACGATGAAACTGCAGACGGCTCATTACAACAGTGATAAACTAAT
AGACTTTCGGGTTTTACCTTTTGGATAACCGCAGTAAATCGGGGCTAATACATACAAGCG
ATGGGTGACTGGCAACGGAAGCTCAGCGATTATTAGCATTCTACCAATGCCTTCGGGTTT
TGGGTGATACCGAATAATATTGCAGATCGAGGATTTATCTTCGACAAGTCTACTGTGTCA
CTGCCCTATCAACTTTCGATGGTACGGTATTGGCCTACCATGGTTGTAACGGGTA-----
-----CTTAGTTGGTGGAGCGATTTGTCTGGTCAATTCCGATAACGGACGAGACCTCGAC
CTGCTAACTAGTAGTATTTATTAGTCGATATAGACGATAGCTTTTCTGGGGTTTGGAATG
ATTTCGGTCATCTCCTGCTTCAAGGAGTGTGTAGTCTGACTCGATAGGTACGAATTAAAA
CTTCTTAGAGGGACTACCTGCCTCAAGCAGGCGGAAGTCCGAGGCAATAACAGGTCTGTG
ATGCCCTTAGATACCTTGGGCCGCACGCGCGCTACAATGTAGGAAACAAAAAGGCTCCTG
GTCCGGAAGGATTGGGTAATCATTTGAATTTCCTACGTAACTGGGCTTGATCTTTGTAAT
TATTGATCATAAACGAGGAATTCCTTGTAAGCGTAAGTCATTACCTTATGCTGAATATGT
CCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATCGAATGATACGGTAAAGTTAA
CGGATCGTTTTATCTGTGGCAACACTGATATAAATTAAAAGTTATTTAAATCTCATTGTT
TAGAGGAAGGAGAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGATGGATCATTTTT
TATCTTAATATCATAAACTTTATGTTTTTTTGGATAATGGAACTGCTTGCGTATAAAGAA
ATTAGGCTTTTTAATTAAGGTTTAAGNAGTAAGTGGNCTGTCAGATTTA