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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE235
Library VH
(Link to library)
Clone ID VHE235
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHE235P
(Link to Original site)
Representative DNA sequence 
>VHE235 (VHE235Q) /CSM/VH/VHE2-B/VHE235Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACACAGTCGATCGTTTCTTATTAXXXXXXXXXXGANTTANGT
TNCCNCANTGCCGGTCAAGNTTTNCCNCAATGNGTTTTNGATCATTGGGNTTCAANTGGN
GTTGTCAACAAAAATAAAAAAGCCNCTGAAGTNGCTTTANCCNCCAAAAAAAAAAAANGT
TTAGCCCCANAAATCCCANATTTTGACAAATTCCNCGAAAAACTNTAAACNATTTAAGTC
GCCCTTTGAGTTTTC
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNTVDRFLL---

---XLXXXXAGQXXPQXVXDHWXSXGVVNKNKKAXEVALXXKKKKXLAPXIPXFDKFXEK
L*ti*val*vf
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstqsivsy---

---XLXXXXAGQXXPQXVXDHWXSXGVVNKNKKAXEVALXXKKKKXLAPXIPXFDKFXEK
L*ti*val*vf

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqhsrsfli---

---xxvxxxpvkxxxnxfxiigxqxxlstkikkplkxlxppkkkxv*pxksxiltnsxkn
xkxfkspfef

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNTVDRFLL---

---xxxpxcrsxfxxmxfxslgfxwxcqqk*ksx*sxfxxqkkkxfspxnpxf*qiprkt
xnxlsrplsf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE235 (VHE235Q) /CSM/VH/VHE2-B/VHE235Q.Seq.d/                       1031   0.0
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/             985   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                        985   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                        985   0.0
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/             985   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                        985   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                        985   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                        985   0.0
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                        985   0.0
VHQ469 (VHQ469Q) /CSM/VH/VHQ4-C/VHQ469Q.Seq.d/                        985   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.   983   0.0    4
DY889811|DY889811.1 CeleSEQ6761 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ6761, mRNA sequence.    84   1e-32  4
DY889772|DY889772.1 CeleSEQ11980 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ11980, mRNA sequence.    84   1e-32  4
DY890935|DY890935.1 CeleSEQ7425 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ7425, mRNA sequence.    84   2e-32  4
DY887441|DY887441.1 CeleSEQ3766 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ3766, mRNA sequence.    84   2e-32  4
DY888936|DY888936.1 CeleSEQ6366 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ6366, mRNA sequence.    84   3e-32  4
CX155337|CX155337.1 TfEST072F03 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    82   9e-32  4
CX154324|CX154324.1 TfEST033C12 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    82   2e-31  4
CX154971|CX154971.1 TfEST089A07 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    82   4e-31  4
CX154356|CX154356.1 TfEST029E10 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    82   5e-31  4
dna update 2007. 6.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   233   6e-60
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   230   4e-59
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   228   1e-58
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   226   6e-58
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   226   6e-58
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   226   6e-58
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   224   2e-57
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   224   2e-57
AY618550_1(AY618550|pid:none) Trypanosoma cruzi elongation facto...   224   2e-57
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   224   2e-57
protein update 2009. 6.30
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHE235 is cyt
5' end seq. ID VHE235F
5' end seq. 
>VHE235F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACACAGTCGATCGTTTCTTATTANNNNNNNNNN
Length of 5' end seq. 531
3' end seq. ID VHE235Z
3' end seq. 
>VHE235Z.Seq
NNNNNNNNNNGANTTANGTTNCCNCANTGCCGGTCAAGNTTTNCCNCAATGNGTTTTNGA
TCATTGGGNTTCAANTGGNGTTGTCAACAAAAATAAAAAAGCCNCTGAAGTNGCTTTANC
CNCCAAAAAAAAAAAANGTTTAGCCCCANAAATCCCANATTTTGACAAATTCCNCGAAAA
ACTNTAAACNATTTAAGTCGCCCTTTGAGTTTTC
Length of 3' end seq. 214
Connected seq. ID VHE235P
Connected seq. 
>VHE235P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACACAGTCGATCGTTTCTTATTA----------GANTTANGT
TNCCNCANTGCCGGTCAAGNTTTNCCNCAATGNGTTTTNGATCATTGGGNTTCAANTGGN
GTTGTCAACAAAAATAAAAAAGCCNCTGAAGTNGCTTTANCCNCCAAAAAAAAAAAANGT
TTAGCCCCANAAATCCCANATTTTGACAAATTCCNCGAAAAACTNTAAACNATTTAAGTC
GCCCTTTGAGTTTTC
Length of connected seq. 725
Full length Seq ID -
Full length Seq. -
Length of full length seq. -