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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE234
Library VH
(Link to library)
Clone ID VHE234
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHE234P
(Link to Original site)
Representative DNA sequence 
>VHE234 (VHE234Q) /CSM/VH/VHE2-B/VHE234Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACX
XXXXXXXXXGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGT
GCTTTCCAATGGGCCACCAAANAAGGTGTTGTTTGTGATGAAAACATGANAGGTATCCGT
TTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACANAGGTGGTGGTCAAATC
ATCCCAACTGCTNGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTTTNTTA
GAACCAATTTACTTAGTAGAAATTACTGCCCCANAAAACGCTATTGGTGGTATTTACTCT
GTACTCAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTNGTATCGGTTCCCCATTA
TTNTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGT
TCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTTTTCGATCATTGGGCTTCAATTGGT
GTTGTCAACAAANATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAANAGAAAAGGT
TTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACNATTTAAGTC
GCNCTTTGAGTTTTCAAAAATAGTAATCAAATCTAAAAAAAATTTTAAAAAAAAAAATA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGN---

---VTKGVQYLNEIKDSFVGAFQWATKXGVVCDENMXGIRFNLYDVTLHTDAIHXGGGQI
IPTAXRVLYAAELTASPTXLEPIYLVEITAPXNAIGGIYSVLNRRXGIVIGEERXIGSPL
XSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKXKKATEVALATRXRKG
LAPEIPDLDKFHEKL*ti*val*vfknsnqi*kkf*kkk
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllv---

---VTKGVQYLNEIKDSFVGAFQWATKXGVVCDENMXGIRFNLYDVTLHTDAIHXGGGQI
IPTAXRVLYAAELTASPTXLEPIYLVEITAPXNAIGGIYSVLNRRXGIVIGEERXIGSPL
XSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKXKKATEVALATRXRKG
LAPEIPDLDKFHEKL*ti*val*vfknsnqi*kkf*kkk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*---

---spkvfni*mklkilslvlsngppxkvlfvmkt*xvsvsifmmspstlmpstxvvvks
sqllvvysmlln*lppqlx*nqft**kllpxktllvvftlysivvxvlllvknvvsvphy
xlskliyqy*npsvspliyvptlpvklshnvfsiiglqlvlstxirkplksl*ppexekv
*pqksqiltnstknskxfksxfefskivikskknfkkkn

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGN---

---hqrcsife*n*rffrwcfpmghqxrccl**khxrypfqsl*chpph*chpxrwwsnh
pncxsctlcc*inclpnfxrtnllsrnycpxkrywwyllctqssxrycyw*rtxyrfpii
xcqssftsirilrfhr*ftfphcrssfptmcfrslgfnwccqqx*esh*srfshqkxkrf
sprnprs*qiprktlnxlsrxlsfqk**snlkkilkkki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE234 (VHE234Q) /CSM/VH/VHE2-B/VHE234Q.Seq.d/                       2270   0.0
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/            1148   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                       1148   0.0
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/            1148   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                       1148   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                       1148   0.0
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                       1148   0.0
VHQ469 (VHQ469Q) /CSM/VH/VHQ4-C/VHQ469Q.Seq.d/                       1148   0.0
VHQ463 (VHQ463Q) /CSM/VH/VHQ4-C/VHQ463Q.Seq.d/                       1148   0.0
VHQ453 (VHQ453Q) /CSM/VH/VHQ4-C/VHQ453Q.Seq.d/                       1148   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.  1146   0.0    1
Y09664|Y09664.1 C.albicans EFT2 gene.                              78   1e-61  8
AF107286|AF107286.1 Candida albicans elongation factor 2 (EFT2) gene, partial cds.    78   4e-61  8
AF107291|AF107291.1 Candida parapsilosis elongation factor 2 (EFT2) gene, partial cds.    66   3e-57  11
AF107289|AF107289.1 Candida tropicalis elongation factor 2 (EFT2) gene, partial cds.    64   6e-56  9
EH015431|EH015431.1 USDA-FP_188032 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt081_G08 5', mRNA sequence.    76   1e-39  4
EH012247|EH012247.1 USDA-FP_185167 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt044_H07 5', mRNA sequence.    76   2e-39  4
AF107290|AF107290.1 Candida lusitaniae elongation factor 2 (EFT2) gene, partial cds.    62   2e-37  7
AR550596|AR550596.1 Sequence 5727 from patent US 6747137.          72   9e-37  5
AY144999|AY144999.1 Saccharomyces kluyveri clone Contig1706 EFT2 gene, complete cds.    78   2e-36  6
dna update 2007. 6.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   263   8e-69
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   258   2e-67
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   256   9e-67
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   256   9e-67
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   256   1e-66
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   256   2e-66
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   256   2e-66
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   254   6e-66
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   253   1e-65
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   253   1e-65
protein update 2009. 6.30
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall

>> prediction for VHE234 is nuc
5' end seq. ID VHE234F
5' end seq. 
>VHE234F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACN
NNNNNNNNN
Length of 5' end seq. 609
3' end seq. ID VHE234Z
3' end seq. 
>VHE234Z.Seq
NNNNNNNNNNGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGG
TGCTTTCCAATGGGCCACCAAANAAGGTGTTGTTTGTGATGAAAACATGANAGGTATCCG
TTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACANAGGTGGTGGTCAAAT
CATCCCAACTGCTNGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTTTNTT
AGAACCAATTTACTTAGTAGAAATTACTGCCCCANAAAACGCTATTGGTGGTATTTACTC
TGTACTCAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTNGTATCGGTTCCCCATT
ATTNTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACG
TTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTTTTCGATCATTGGGCTTCAATTGG
TGTTGTCAACAAANATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAANAGAAAAGG
TTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACNATTTAAGT
CGCNCTTTGAGTTTTCAAAAATAGTAATCAAATCTAAAAAAAATTTTAAAAAAAAAAATA
Length of 3' end seq. 660
Connected seq. ID VHE234P
Connected seq. 
>VHE234P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAAC-
---------GTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGT
GCTTTCCAATGGGCCACCAAANAAGGTGTTGTTTGTGATGAAAACATGANAGGTATCCGT
TTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACANAGGTGGTGGTCAAATC
ATCCCAACTGCTNGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTTTNTTA
GAACCAATTTACTTAGTAGAAATTACTGCCCCANAAAACGCTATTGGTGGTATTTACTCT
GTACTCAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTNGTATCGGTTCCCCATTA
TTNTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGT
TCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTTTTCGATCATTGGGCTTCAATTGGT
GTTGTCAACAAANATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAANAGAAAAGGT
TTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACNATTTAAGTC
GCNCTTTGAGTTTTCAAAAATAGTAATCAAATCTAAAAAAAATTTTAAAAAAAAAAATA
Length of connected seq. 1249
Full length Seq ID -
Full length Seq. -
Length of full length seq. -