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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE229
Library VH
(Link to library)
Clone ID VHE229
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHE229P
(Link to Original site)
Representative DNA sequence 
>VHE229 (VHE229Q) /CSM/VH/VHE2-B/VHE229Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCCAGXXXXXXXXXXGTCTTTCGGTC
CAGAAGGTAACGGTGCCAATCTCNTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATG
AAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTG
ATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGANGNTCACCCTCCACACTGAGATG
CCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTG
AATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAG
AAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTG
AAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAAT
CCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTG
TCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCG
CTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTGACAAATTCCA
CGAAAAACTCTAAACAATTTAAGTCGCACTTGAGTTTTCAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVTVSP---

---SFGPEGNGANLXVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYXX
HPPH*daihrgggqiiptarrvlyaaeltasptllepiylveitapenaiggiysvlnrr
rgivigeerrigsplfsvkahlpvlesfgftadlrshtagqafpqcvfdhwasigvvnkd
kkatevalatrkrkglapeipdltnstknskqfkshlsfq
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlkirnsvmslypq---

---vfrsrr*rcqsxrqchqrcsife*n*rffrwcfpmghqrrccl**kherypfqsl*x
spstlrchpqrwwsnhpncssctlcc*inclpnslrtnllsrnycprkrywwyllctqss
*rycyw*rtsyrfpiilcqssftsirilrfhr*ftfphcrssfptmclrslgfnwccqqr
*esh*srfshqkekrfsprnprsdkfhekl*ti*valefs

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r*gir*chcip---

---SFGPEGNGANLXVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYXX
HPPH*daihrgggqiiptarrvlyaaeltasptllepiylveitapenaiggiysvlnrr
rgivigeerrigsplfsvkahlpvlesfgftadlrshtagqafpqcvfdhwasigvvnkd
kkatevalatrkrkglapeipdltnstknskqfkshlsfq

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVTVSP---

---lsvqkvtvpisssmspkvfni*mklkilslvlsngppkkvlfvmkt*evsvsifmxx
tlhtempstevvvkssqllvvysmlln*lppqls*nqst**kllpqktllvvftlysivv
evlllvknvvsvphyslskliyqy*npsvspliyvptlpvklshnvssiiglqlvlstki
rkplksl*pperekv*pqksqi*qiprktlnnlsrt*vfk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE229 (VHE229Q) /CSM/VH/VHE2-B/VHE229Q.Seq.d/                       2464   0.0
VHO564 (VHO564Q) /CSM/VH/VHO5-C/VHO564Q.Seq.d/                       1245   0.0
VHK412 (VHK412Q) /CSM/VH/VHK4-A/VHK412Q.Seq.d/                       1245   0.0
VHC423 (VHC423Q) /CSM/VH/VHC4-A/VHC423Q.Seq.d/                       1245   0.0
VHB461 (VHB461Q) /CSM/VH/VHB4-C/VHB461Q.Seq.d/                       1245   0.0
VHA491 (VHA491Q) /CSM/VH/VHA4-D/VHA491Q.Seq.d/                       1245   0.0
VHN246 (VHN246Q) /CSM/VH/VHN2-B/VHN246Q.Seq.d/                       1239   0.0
VHM870 (VHM870Q) /CSM/VH/VHM8-C/VHM870Q.Seq.d/                       1239   0.0
VHM618 (VHM618Q) /CSM/VH/VHM6-A/VHM618Q.Seq.d/                       1239   0.0
VHM590 (VHM590Q) /CSM/VH/VHM5-D/VHM590Q.Seq.d/                       1239   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.  1223   0.0    1
AF107286|AF107286.1 Candida albicans elongation factor 2 (EFT2) gene, partial cds.    78   7e-69  9
AF107291|AF107291.1 Candida parapsilosis elongation factor 2 (EFT2) gene, partial cds.    68   1e-67  13
Y09664|Y09664.1 C.albicans EFT2 gene.                              78   2e-67  9
AF107289|AF107289.1 Candida tropicalis elongation factor 2 (EFT2) gene, partial cds.    70   4e-62  10
AF107290|AF107290.1 Candida lusitaniae elongation factor 2 (EFT2) gene, partial cds.    62   1e-45  8
AY144999|AY144999.1 Saccharomyces kluyveri clone Contig1706 EFT2 gene, complete cds.    78   2e-43  7
AR550596|AR550596.1 Sequence 5727 from patent US 6747137.          78   1e-42  6
AF534908|AF534908.2 Tetrahymena thermophila elongation factor 2 gene, complete cds.    84   1e-42  5
AY144923|AY144923.1 Saccharomyces castellii clone Contig2043 EFT gene, complete cds.    54   2e-40  8
dna update 2007. 6.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB171046_1(AB171046|pid:none) Macaca fascicularis brain cDNA clo...   401   e-110
AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   273   1e-71
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   268   4e-70
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   267   7e-70
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   266   2e-69
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   266   2e-69
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   266   2e-69
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   265   2e-69
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   265   3e-69
AB095183_1(AB095183|pid:none) Cyanidioschyzon merolae gene for e...   263   1e-68
protein update 2009. 6.30
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHE229 is cyt
5' end seq. ID VHE229F
5' end seq. 
>VHE229F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCCAGNNNNNNNNNN
Length of 5' end seq. 649
3' end seq. ID VHE229Z
3' end seq. 
>VHE229Z.Seq
NNNNNNNNNNGTCTTTCGGTCCAGAAGGTAACGGTGCCAATCTCNTCGTCAATGTCACCA
AAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCA
CCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGANG
NTCACCCTCCACACTGAGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTC
GTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACT
TAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTC
GTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAG
CTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCG
GTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAG
ATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAA
TCCCAGATCTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTGAGTTTTC
AAA
Length of 3' end seq. 663
Connected seq. ID VHE229P
Connected seq. 
>VHE229P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCCAG----------GTCTTTCGGTC
CAGAAGGTAACGGTGCCAATCTCNTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATG
AAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTG
ATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGANGNTCACCCTCCACACTGAGATG
CCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTG
AATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAG
AAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTG
AAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAAT
CCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTG
TCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCG
CTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTGACAAATTCCA
CGAAAAACTCTAAACAATTTAAGTCGCACTTGAGTTTTCAAA
Length of connected seq. 1292
Full length Seq ID -
Full length Seq. -
Length of full length seq. -